UniProtKB - Q6RUV5 (RAC1_RAT)
Ras-related C3 botulinum toxin substrate 1
Rac1
Functioni
Catalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 60 | GTP; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 13 – 18 | GTPBy similarity | 6 | |
Nucleotide bindingi | 30 – 35 | GTPBy similarity | 6 | |
Nucleotide bindingi | 116 – 118 | GTPBy similarity | 3 | |
Nucleotide bindingi | 159 – 160 | GTPBy similarity | 2 |
GO - Molecular functioni
- ATPase binding Source: RGD
- enzyme binding Source: RGD
- GTPase activity Source: RGD
- GTP binding Source: RGD
- GTP-dependent protein binding Source: RGD
- histone deacetylase binding Source: RGD
- protein-containing complex binding Source: RGD
- protein kinase binding Source: BHF-UCL
- Rab GTPase binding Source: RGD
- Rho GDP-dissociation inhibitor binding Source: RGD
- thioesterase binding Source: RGD
GO - Biological processi
- actin cytoskeleton organization Source: RGD
- actin filament organization Source: RGD
- actin filament polymerization Source: RGD
- anatomical structure arrangement Source: RGD
- auditory receptor cell morphogenesis Source: RGD
- axon guidance Source: RGD
- bone resorption Source: RGD
- cell adhesion Source: RGD
- cell-cell junction organization Source: RGD
- cell migration Source: RGD
- cell motility Source: UniProtKB
- cell population proliferation Source: RGD
- cell projection assembly Source: GO_Central
- cellular response to mechanical stimulus Source: RGD
- cerebral cortex GABAergic interneuron development Source: RGD
- cerebral cortex radially oriented cell migration Source: RGD
- chemotaxis Source: RGD
- cochlea morphogenesis Source: RGD
- cortical cytoskeleton organization Source: GO_Central
- cytoskeleton organization Source: RGD
- dendrite development Source: RGD
- dendrite morphogenesis Source: RGD
- dopaminergic neuron differentiation Source: RGD
- embryonic olfactory bulb interneuron precursor migration Source: RGD
- endocytosis Source: RGD
- engulfment of apoptotic cell Source: RGD
- epithelial cell morphogenesis Source: RGD
- establishment or maintenance of cell polarity Source: GO_Central
- forebrain development Source: RGD
- G protein-coupled receptor signaling pathway Source: RGD
- hepatocyte growth factor receptor signaling pathway Source: RGD
- homeostasis of number of cells within a tissue Source: RGD
- hyperosmotic response Source: RGD
- lamellipodium assembly Source: RGD
- localization within membrane Source: RGD
- mast cell chemotaxis Source: RGD
- midbrain dopaminergic neuron differentiation Source: RGD
- motor neuron axon guidance Source: GO_Central
- negative regulation of interleukin-23 production Source: RGD
- neuron migration Source: UniProtKB
- neuron projection morphogenesis Source: RGD
- non-canonical Wnt signaling pathway Source: RGD
- phagocytosis, engulfment Source: RGD
- positive regulation of actin filament polymerization Source: RGD
- positive regulation of cell-substrate adhesion Source: RGD
- positive regulation of dendritic spine development Source: RGD
- positive regulation of DNA replication Source: RGD
- positive regulation of focal adhesion assembly Source: RGD
- positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: RGD
- positive regulation of lamellipodium assembly Source: RGD
- positive regulation of microtubule polymerization Source: RGD
- positive regulation of neutrophil chemotaxis Source: RGD
- positive regulation of ovarian follicle development Source: RGD
- positive regulation of phosphatidylinositol 3-kinase activity Source: RGD
- positive regulation of protein phosphorylation Source: UniProtKB
- positive regulation of stress fiber assembly Source: RGD
- positive regulation of substrate adhesion-dependent cell spreading Source: RGD
- protein localization to plasma membrane Source: RGD
- Rac protein signal transduction Source: SynGO
- regulation of actin cytoskeleton organization Source: GO_Central
- regulation of cell adhesion involved in heart morphogenesis Source: RGD
- regulation of cell migration Source: UniProtKB
- regulation of cell morphogenesis Source: RGD
- regulation of cell shape Source: GO_Central
- regulation of cell size Source: RGD
- regulation of fibroblast migration Source: RGD
- regulation of lamellipodium assembly Source: RGD
- regulation of neuronal synaptic plasticity Source: RGD
- regulation of neuron maturation Source: RGD
- regulation of neutrophil migration Source: GO_Central
- regulation of nitric oxide biosynthetic process Source: ARUK-UCL
- regulation of receptor signaling pathway via JAK-STAT Source: RGD
- regulation of respiratory burst Source: RGD
- regulation of stress fiber assembly Source: RGD
- ruffle assembly Source: RGD
- ruffle organization Source: RGD
- semaphorin-plexin signaling pathway Source: UniProtKB
- small GTPase mediated signal transduction Source: RGD
- substrate adhesion-dependent cell spreading Source: UniProtKB
- synaptic transmission, GABAergic Source: RGD
- Wnt signaling pathway, planar cell polarity pathway Source: RGD
Keywordsi
Molecular function | Hydrolase |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-114604, GPVI-mediated activation cascade R-RNO-1257604, PIP3 activates AKT signaling R-RNO-1433557, Signaling by SCF-KIT R-RNO-193648, NRAGE signals death through JNK R-RNO-194840, Rho GTPase cycle R-RNO-2029482, Regulation of actin dynamics for phagocytic cup formation R-RNO-2424491, DAP12 signaling R-RNO-2871796, FCERI mediated MAPK activation R-RNO-376172, DSCAM interactions R-RNO-389359, CD28 dependent Vav1 pathway R-RNO-3928662, EPHB-mediated forward signaling R-RNO-3928664, Ephrin signaling R-RNO-3928665, EPH-ephrin mediated repulsion of cells R-RNO-399954, Sema3A PAK dependent Axon repulsion R-RNO-4086400, PCP/CE pathway R-RNO-416550, Sema4D mediated inhibition of cell attachment and migration R-RNO-418885, DCC mediated attractive signaling R-RNO-4420097, VEGFA-VEGFR2 Pathway R-RNO-445144, Signal transduction by L1 R-RNO-5218920, VEGFR2 mediated vascular permeability R-RNO-5625740, RHO GTPases activate PKNs R-RNO-5625900, RHO GTPases activate CIT R-RNO-5625970, RHO GTPases activate KTN1 R-RNO-5626467, RHO GTPases activate IQGAPs R-RNO-5627123, RHO GTPases activate PAKs R-RNO-5663220, RHO GTPases Activate Formins R-RNO-5668599, RHO GTPases Activate NADPH Oxidases R-RNO-5687128, MAPK6/MAPK4 signaling R-RNO-6798695, Neutrophil degranulation R-RNO-6811558, PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling R-RNO-8849471, PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases R-RNO-8875555, MET activates RAP1 and RAC1 R-RNO-9032759, NTRK2 activates RAC1 R-RNO-983231, Factors involved in megakaryocyte development and platelet production |
Names & Taxonomyi
Protein namesi | Recommended name: Ras-related C3 botulinum toxin substrate 1Curated (EC:3.6.5.21 Publication)Alternative name(s): p21-Rac1 |
Gene namesi | Name:Rac1Imported |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619755, Rac1 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication
Other locations
- Melanosome By similarity
- Cytoplasm By similarity
- lamellipodium By similarity
Note: Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Localizes to the lamellipodium in a SH3RF1-dependent manner. In macrophages, cytoplasmic location increases upon CSF1 stimulation (By similarity).By similarity
Cytoskeleton
- actin filament Source: Ensembl
- cytoskeleton Source: GO_Central
- pericentriolar material Source: RGD
Cytosol
- cytosol Source: UniProtKB
Endosome
- early endosome membrane Source: RGD
- recycling endosome membrane Source: RGD
Golgi apparatus
- Golgi membrane Source: RGD
- trans-Golgi network Source: RGD
Nucleus
- nucleus Source: RGD
Plasma Membrane
- extrinsic component of plasma membrane Source: RGD
- phagocytic cup Source: RGD
- plasma membrane Source: RGD
- ruffle membrane Source: RGD
Other locations
- cell cortex Source: GO_Central
- cell projection Source: RGD
- cytoplasm Source: RGD
- cytoplasmic ribonucleoprotein granule Source: RGD
- cytoplasmic vesicle Source: RGD
- dendritic spine Source: SynGO
- glutamatergic synapse Source: RGD
- intracellular membrane-bounded organelle Source: GO_Central
- kinocilium Source: RGD
- lamellipodium Source: UniProtKB
- melanosome Source: UniProtKB-SubCell
- membrane Source: UniProtKB
- postsynapse Source: RGD
- vesicle Source: GO_Central
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 12 | G → V: Constitutively active. Increases PAK1 and LIMK1 phosphorylation and NR3C2 nuclear localization in podocytes. 1 Publication | 1 | |
Mutagenesisi | 17 | T → N: Dominant negatif. Reduces NMDA receptor-mediated synaptic currents. 1 Publication | 1 | |
Mutagenesisi | 61 | Q → L: Constitutively active. Interacts with PPP5C. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000042040 | 1 – 189 | Ras-related C3 botulinum toxin substrate 1Add BLAST | 189 | |
PropeptideiPRO_0000042041 | 190 – 192 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 147 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 189 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 189 | S-geranylgeranyl cysteineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Lipoprotein, Methylation, Prenylation, Ubl conjugationProteomic databases
jPOSTi | Q6RUV5 |
PaxDbi | Q6RUV5 |
PRIDEi | Q6RUV5 |
PTM databases
iPTMneti | Q6RUV5 |
PhosphoSitePlusi | Q6RUV5 |
SwissPalmi | Q6RUV5 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000001068, Expressed in cerebellum and 21 other tissues |
ExpressionAtlasi | Q6RUV5, baseline and differential |
Genevisiblei | Q6RUV5, RN |
Interactioni
Subunit structurei
Interacts with NISCH.
Interacts with PIP5K1A.
Interacts with the GTP-bound form of RAB7A.
Interacts with SRGAP2.
Interacts with CYFIP1/SRA-1.
Interacts with PLXNB3.
Interacts with ARHGDIA; the interaction is induced by SEMA5A, mediated through PLXNB3 and inactivates and stabilizes RAC1.
Interacts (GTP-bound form preferentially) with PKN2 (via the REM repeats); the interaction stimulates autophosphorylation and phosphorylation of PKN2.
Interacts with the GEF proteins PREX1, RASGRF2, FARP1, FARP2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it.
Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner.
Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization.
Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells.
Interacts with RALBP1 via its effector domain.
Interacts with PLXNB1. Probably found in a ternary complex composed of DSCAM, PAK1 and RAC1.
Interacts with DSCAM; the interaction requires PAK1.
Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6.
Interacts with BAIAP2, BAIAP2L1 and DEF6.
Interacts with Y.pseudotuberculosis YPKA and PLCB2.
Interacts with NOXA1.
Interacts with ARHGEF2.
Interacts with TBC1D2.
Interacts with UNKL.
Interacts with USP6.
Interacts with SPATA13.
Interacts with ARHGEF16; mediates activation of RAC1 by EPHA2.
Interacts with ITGB4.
Interacts with S100A8 and calprotectin (S100A8/9).
Interacts with PACSIN2.
Interacts with ITGB1BP1.
Interacts (when active) with PPP5C (via TPR repeats); activates PPP5C phosphatase activity and translocates PPP5C to the cell membrane.
Interacts with RAPH1 (via Ras associating and PH domains) (By similarity).
Interacts with MTSS2 (via IMD domain); this interaction may be important to potentiate PDGF-induced RAC1 activation.
Interacts with PAK2.
Interacts (GTP-bound form) with SH3RF1 and SH3RF3.
Found in a complex with SH3RF1, MAPK8IP1/JIP1, MAP3K11/MLK3, MAP2K7/MKK7 and MAPK8/JNK1 (By similarity).
Interacts (both active GTP- or inactive GDP-bound forms) with SH3RF2 (PubMed:22128169).
Interacts (GTP-bound form preferentially) with CYRIB (By similarity).
Interacts with DOCK4 (via DOCKER domain); functions as a guanine nucleotide exchange factor (GEF) for RAC1 (By similarity).
Interacts with GARRE1 (By similarity).
By similarity4 PublicationsGO - Molecular functioni
- ATPase binding Source: RGD
- enzyme binding Source: RGD
- GTP-dependent protein binding Source: RGD
- histone deacetylase binding Source: RGD
- protein kinase binding Source: BHF-UCL
- Rab GTPase binding Source: RGD
- Rho GDP-dissociation inhibitor binding Source: RGD
- thioesterase binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 264467, 9 interactors |
CORUMi | Q6RUV5 |
DIPi | DIP-37114N |
IntActi | Q6RUV5, 4 interactors |
STRINGi | 10116.ENSRNOP00000001417 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 32 – 40 | Effector regionSequence analysis | 9 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0393, Eukaryota |
GeneTreei | ENSGT00940000153500 |
HOGENOMi | CLU_041217_21_3_1 |
InParanoidi | Q6RUV5 |
OrthoDBi | 1091615at2759 |
PhylomeDBi | Q6RUV5 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR003578, Small_GTPase_Rho |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51420, RHO, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP
60 70 80 90 100
VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE
110 120 130 140 150
VRHHCPNTPI ILVGTKLDLR DDKDTIEKLK EKKLTPITYP QGLAMAKEIG
160 170 180 190
AVKYLECSAL TQRGLKTVFD EAIRAVLCPP PVKKRKRKCL LL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K0X4 | A0A0G2K0X4_RAT | Ras-related C3 botulinum toxin subs... | Rac1 | 217 | Annotation score: | ||
A0A0U1RS21 | A0A0U1RS21_RAT | Ras-related C3 botulinum toxin subs... | Rac1 | 115 | Annotation score: | ||
A0A0U1RS18 | A0A0U1RS18_RAT | Ras-related C3 botulinum toxin subs... | Rac1 | 22 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY491395 mRNA Translation: AAR84574.1 |
RefSeqi | NP_599193.1, NM_134366.1 |
Genome annotation databases
Ensembli | ENSRNOT00000001417; ENSRNOP00000001417; ENSRNOG00000001068 |
GeneIDi | 363875 |
KEGGi | rno:363875 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY491395 mRNA Translation: AAR84574.1 |
RefSeqi | NP_599193.1, NM_134366.1 |
3D structure databases
BMRBi | Q6RUV5 |
SMRi | Q6RUV5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 264467, 9 interactors |
CORUMi | Q6RUV5 |
DIPi | DIP-37114N |
IntActi | Q6RUV5, 4 interactors |
STRINGi | 10116.ENSRNOP00000001417 |
PTM databases
iPTMneti | Q6RUV5 |
PhosphoSitePlusi | Q6RUV5 |
SwissPalmi | Q6RUV5 |
Proteomic databases
jPOSTi | Q6RUV5 |
PaxDbi | Q6RUV5 |
PRIDEi | Q6RUV5 |
Genome annotation databases
Ensembli | ENSRNOT00000001417; ENSRNOP00000001417; ENSRNOG00000001068 |
GeneIDi | 363875 |
KEGGi | rno:363875 |
Organism-specific databases
CTDi | 5879 |
RGDi | 619755, Rac1 |
Phylogenomic databases
eggNOGi | KOG0393, Eukaryota |
GeneTreei | ENSGT00940000153500 |
HOGENOMi | CLU_041217_21_3_1 |
InParanoidi | Q6RUV5 |
OrthoDBi | 1091615at2759 |
PhylomeDBi | Q6RUV5 |
Enzyme and pathway databases
Reactomei | R-RNO-114604, GPVI-mediated activation cascade R-RNO-1257604, PIP3 activates AKT signaling R-RNO-1433557, Signaling by SCF-KIT R-RNO-193648, NRAGE signals death through JNK R-RNO-194840, Rho GTPase cycle R-RNO-2029482, Regulation of actin dynamics for phagocytic cup formation R-RNO-2424491, DAP12 signaling R-RNO-2871796, FCERI mediated MAPK activation R-RNO-376172, DSCAM interactions R-RNO-389359, CD28 dependent Vav1 pathway R-RNO-3928662, EPHB-mediated forward signaling R-RNO-3928664, Ephrin signaling R-RNO-3928665, EPH-ephrin mediated repulsion of cells R-RNO-399954, Sema3A PAK dependent Axon repulsion R-RNO-4086400, PCP/CE pathway R-RNO-416550, Sema4D mediated inhibition of cell attachment and migration R-RNO-418885, DCC mediated attractive signaling R-RNO-4420097, VEGFA-VEGFR2 Pathway R-RNO-445144, Signal transduction by L1 R-RNO-5218920, VEGFR2 mediated vascular permeability R-RNO-5625740, RHO GTPases activate PKNs R-RNO-5625900, RHO GTPases activate CIT R-RNO-5625970, RHO GTPases activate KTN1 R-RNO-5626467, RHO GTPases activate IQGAPs R-RNO-5627123, RHO GTPases activate PAKs R-RNO-5663220, RHO GTPases Activate Formins R-RNO-5668599, RHO GTPases Activate NADPH Oxidases R-RNO-5687128, MAPK6/MAPK4 signaling R-RNO-6798695, Neutrophil degranulation R-RNO-6811558, PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling R-RNO-8849471, PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases R-RNO-8875555, MET activates RAP1 and RAC1 R-RNO-9032759, NTRK2 activates RAC1 R-RNO-983231, Factors involved in megakaryocyte development and platelet production |
Miscellaneous databases
PROi | PR:Q6RUV5 |
Gene expression databases
Bgeei | ENSRNOG00000001068, Expressed in cerebellum and 21 other tissues |
ExpressionAtlasi | Q6RUV5, baseline and differential |
Genevisiblei | Q6RUV5, RN |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR003578, Small_GTPase_Rho |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51420, RHO, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAC1_RAT | |
Accessioni | Q6RUV5Primary (citable) accession number: Q6RUV5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 31, 2004 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families