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Protein

Ras-related C3 botulinum toxin substrate 1

Gene

Rac1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3.By similarity5 Publications

Activity regulationi

Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. GTP hydrolysis is stimulated by ARHGAP30 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 18GTPBy similarity6
Nucleotide bindingi30 – 35GTPBy similarity6
Nucleotide bindingi116 – 118GTPBy similarity3
Nucleotide bindingi159 – 160GTPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-114604 GPVI-mediated activation cascade
R-RNO-1433557 Signaling by SCF-KIT
R-RNO-193648 NRAGE signals death through JNK
R-RNO-194840 Rho GTPase cycle
R-RNO-2029482 Regulation of actin dynamics for phagocytic cup formation
R-RNO-2424491 DAP12 signaling
R-RNO-2871796 FCERI mediated MAPK activation
R-RNO-376172 DSCAM interactions
R-RNO-389359 CD28 dependent Vav1 pathway
R-RNO-3928662 EPHB-mediated forward signaling
R-RNO-3928664 Ephrin signaling
R-RNO-3928665 EPH-ephrin mediated repulsion of cells
R-RNO-399954 Sema3A PAK dependent Axon repulsion
R-RNO-4086400 PCP/CE pathway
R-RNO-416482 G alpha (12/13) signalling events
R-RNO-416550 Sema4D mediated inhibition of cell attachment and migration
R-RNO-418885 DCC mediated attractive signaling
R-RNO-4420097 VEGFA-VEGFR2 Pathway
R-RNO-445144 Signal transduction by L1
R-RNO-5218920 VEGFR2 mediated vascular permeability
R-RNO-5625740 RHO GTPases activate PKNs
R-RNO-5625900 RHO GTPases activate CIT
R-RNO-5625970 RHO GTPases activate KTN1
R-RNO-5626467 RHO GTPases activate IQGAPs
R-RNO-5627123 RHO GTPases activate PAKs
R-RNO-5663213 RHO GTPases Activate WASPs and WAVEs
R-RNO-5663220 RHO GTPases Activate Formins
R-RNO-5668599 RHO GTPases Activate NADPH Oxidases
R-RNO-5687128 MAPK6/MAPK4 signaling
R-RNO-6798695 Neutrophil degranulation
R-RNO-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases
R-RNO-8875555 MET activates RAP1 and RAC1
R-RNO-9032759 NTRK2 activates RAC1
R-RNO-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related C3 botulinum toxin substrate 1
Alternative name(s):
p21-Rac1
Gene namesi
Name:Rac1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi619755 Rac1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi12G → V: Constitutively active. Increases PAK1 and LIMK1 phosphorylation and NR3C2 nuclear localization in podocytes. 1 Publication1
Mutagenesisi17T → N: Dominant negatif. Reduces NMDA receptor-mediated synaptic currents. 1 Publication1
Mutagenesisi61Q → L: Constitutively active. Interacts with PPP5C. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000420401 – 189Ras-related C3 botulinum toxin substrate 1Add BLAST189
PropeptideiPRO_0000042041190 – 192Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki147Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei189Cysteine methyl esterBy similarity1
Lipidationi189S-geranylgeranyl cysteineBy similarity1

Post-translational modificationi

GTP-bound active form is ubiquitinated by HACE1, leading to its degradation by the proteasome.By similarity

Keywords - PTMi

Isopeptide bond, Lipoprotein, Methylation, Prenylation, Ubl conjugation

Proteomic databases

PaxDbiQ6RUV5
PRIDEiQ6RUV5

PTM databases

iPTMnetiQ6RUV5
PhosphoSitePlusiQ6RUV5
SwissPalmiQ6RUV5

Expressioni

Tissue specificityi

Osteoclasts.1 Publication

Gene expression databases

BgeeiENSRNOG00000001068 Expressed in 10 organ(s), highest expression level in lung
ExpressionAtlasiQ6RUV5 baseline and differential
GenevisibleiQ6RUV5 RN

Interactioni

Subunit structurei

Interacts with NISCH. Interacts with PIP5K1A. Interacts with the GTP-bound form of RAB7A. Interacts with SRGAP2. Interacts with CYFIP1/SRA-1. Interacts with PLXNB3. Interacts with ARHGDIA; the interaction is induced by SEMA5A, mediated through PLXNB3 and inactivates and stabilizes RAC1. Interacts (GTP-bound form preferentially) with PKN2 (via the REM repeats); the interaction stimulates autophosphorylation and phosphorylation of PKN2. Interacts with the GEF proteins PREX1, RASGRF2, FARP1, FARP2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Probably found in a ternary complex composed of DSCAM, PAK1 and RAC1. Interacts with DSCAM; the interaction requires PAK1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, BAIAP2L1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2. Interacts with NOXA1. Interacts with ARHGEF2. Interacts with TBC1D2. Interacts with UNKL. Interacts with USP6. Interacts with SPATA13. Interacts with ARHGEF16; mediates activation of RAC1 by EPHA2. Interacts with ITGB4. Interacts with S100A8 and calprotectin (S100A8/9). Interacts with PACSIN2. Interacts with ITGB1BP1. Interacts (when active) with PPP5C (via TPR repeats); activates PPP5C phosphatase activity and translocates PPP5C to the cell membrane. Interacts with RAPH1 (via Ras associating and PH domains) (By similarity). Interacts with MTSS1L (via IMD domain); this interaction may be important to potentiate PDGF-induced RAC1 activation. Interacts with PAK2. Interacts (GTP-bound form) with SH3RF1 and SH3RF3. Found in a complex with SH3RF1, MAPK8IP1/JIP1, MAP3K11/MLK3, MAP2K7/MKK7 and MAPK8/JNK1 (By similarity). Interacts (both active GTP- or inactive GDP-bound forms) with SH3RF2 (PubMed:22128169).By similarity4 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi264467, 6 interactors
CORUMiQ6RUV5
DIPiDIP-37114N
IntActiQ6RUV5, 4 interactors
STRINGi10116.ENSRNOP00000001417

Structurei

3D structure databases

ProteinModelPortaliQ6RUV5
SMRiQ6RUV5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector regionSequence analysis9

Domaini

The effector region mediates interaction with DEF6.By similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118978
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiQ6RUV5
KOiK04392
PhylomeDBiQ6RUV5

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q6RUV5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP
60 70 80 90 100
VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE
110 120 130 140 150
VRHHCPNTPI ILVGTKLDLR DDKDTIEKLK EKKLTPITYP QGLAMAKEIG
160 170 180 190
AVKYLECSAL TQRGLKTVFD EAIRAVLCPP PVKKRKRKCL LL
Length:192
Mass (Da):21,450
Last modified:July 5, 2004 - v1
Checksum:iACEDF83A45E5EA67
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2K0X4A0A0G2K0X4_RAT
Ras-related C3 botulinum toxin subs...
Rac1
217Annotation score:
A0A0U1RS21A0A0U1RS21_RAT
Ras-related C3 botulinum toxin subs...
Rac1
115Annotation score:
A0A0U1RS18A0A0U1RS18_RAT
Ras-related C3 botulinum toxin subs...
Rac1
22Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY491395 mRNA Translation: AAR84574.1
RefSeqiNP_599193.1, NM_134366.1
UniGeneiRn.228585
Rn.29157

Genome annotation databases

EnsembliENSRNOT00000001417; ENSRNOP00000001417; ENSRNOG00000001068
GeneIDi363875
KEGGirno:363875

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY491395 mRNA Translation: AAR84574.1
RefSeqiNP_599193.1, NM_134366.1
UniGeneiRn.228585
Rn.29157

3D structure databases

ProteinModelPortaliQ6RUV5
SMRiQ6RUV5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi264467, 6 interactors
CORUMiQ6RUV5
DIPiDIP-37114N
IntActiQ6RUV5, 4 interactors
STRINGi10116.ENSRNOP00000001417

PTM databases

iPTMnetiQ6RUV5
PhosphoSitePlusiQ6RUV5
SwissPalmiQ6RUV5

Proteomic databases

PaxDbiQ6RUV5
PRIDEiQ6RUV5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001417; ENSRNOP00000001417; ENSRNOG00000001068
GeneIDi363875
KEGGirno:363875

Organism-specific databases

CTDi5879
RGDi619755 Rac1

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118978
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiQ6RUV5
KOiK04392
PhylomeDBiQ6RUV5

Enzyme and pathway databases

ReactomeiR-RNO-114604 GPVI-mediated activation cascade
R-RNO-1433557 Signaling by SCF-KIT
R-RNO-193648 NRAGE signals death through JNK
R-RNO-194840 Rho GTPase cycle
R-RNO-2029482 Regulation of actin dynamics for phagocytic cup formation
R-RNO-2424491 DAP12 signaling
R-RNO-2871796 FCERI mediated MAPK activation
R-RNO-376172 DSCAM interactions
R-RNO-389359 CD28 dependent Vav1 pathway
R-RNO-3928662 EPHB-mediated forward signaling
R-RNO-3928664 Ephrin signaling
R-RNO-3928665 EPH-ephrin mediated repulsion of cells
R-RNO-399954 Sema3A PAK dependent Axon repulsion
R-RNO-4086400 PCP/CE pathway
R-RNO-416482 G alpha (12/13) signalling events
R-RNO-416550 Sema4D mediated inhibition of cell attachment and migration
R-RNO-418885 DCC mediated attractive signaling
R-RNO-4420097 VEGFA-VEGFR2 Pathway
R-RNO-445144 Signal transduction by L1
R-RNO-5218920 VEGFR2 mediated vascular permeability
R-RNO-5625740 RHO GTPases activate PKNs
R-RNO-5625900 RHO GTPases activate CIT
R-RNO-5625970 RHO GTPases activate KTN1
R-RNO-5626467 RHO GTPases activate IQGAPs
R-RNO-5627123 RHO GTPases activate PAKs
R-RNO-5663213 RHO GTPases Activate WASPs and WAVEs
R-RNO-5663220 RHO GTPases Activate Formins
R-RNO-5668599 RHO GTPases Activate NADPH Oxidases
R-RNO-5687128 MAPK6/MAPK4 signaling
R-RNO-6798695 Neutrophil degranulation
R-RNO-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases
R-RNO-8875555 MET activates RAP1 and RAC1
R-RNO-9032759 NTRK2 activates RAC1
R-RNO-983231 Factors involved in megakaryocyte development and platelet production

Miscellaneous databases

PROiPR:Q6RUV5

Gene expression databases

BgeeiENSRNOG00000001068 Expressed in 10 organ(s), highest expression level in lung
ExpressionAtlasiQ6RUV5 baseline and differential
GenevisibleiQ6RUV5 RN

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAC1_RAT
AccessioniPrimary (citable) accession number: Q6RUV5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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