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Protein

Leucine-rich repeat transmembrane protein FLRT1

Gene

Flrt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in fibroblast growth factor-mediated signaling cascades that lead to the activation of MAP kinases (PubMed:16872596, PubMed:20421966). Promotes neurite outgrowth via FGFR1-mediated activation of downstream MAP kinases. Promotes an increase both in neurite number and in neurite length (PubMed:20421966). May play a role in cell-cell adhesion and cell guidance via its interaction with ADGRL1/LPHN1 and ADGRL3 (PubMed:22405201).1 Publication2 Publications

GO - Molecular functioni

  • fibroblast growth factor receptor binding Source: UniProtKB

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • dendrite development Source: UniProtKB
  • fibroblast growth factor receptor signaling pathway Source: UniProtKB
  • neuron projection extension Source: UniProtKB
  • positive regulation of synapse assembly Source: MGI

Keywordsi

Molecular functionDevelopmental protein
Biological processCell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-5654687 Downstream signaling of activated FGFR1

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat transmembrane protein FLRT1
Alternative name(s):
Fibronectin leucine rich transmembrane protein 1Imported
Gene namesi
Name:Flrt1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:3026647 Flrt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 552ExtracellularCuratedAdd BLAST532
Transmembranei553 – 573HelicalSequence analysisAdd BLAST21
Topological domaini574 – 674CytoplasmicCuratedAdd BLAST101

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi600Y → F: Decreases phosphorylation. Nearly abolishes phosphorylation; when associated with F-633 and F-671. 1 Publication1
Mutagenesisi633Y → F: Decreases phosphorylation. Nearly abolishes phosphorylation; when associated with F-600 and F-671. 1 Publication1
Mutagenesisi671Y → F: Decreases phosphorylation. Nearly abolishes phosphorylation; when associated with F-600 and F-633. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000043452221 – 674Leucine-rich repeat transmembrane protein FLRT1Add BLAST654

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi54 ↔ 60By similarity
Disulfide bondi58 ↔ 67By similarity
Glycosylationi305N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi332 ↔ 357By similarity
Modified residuei600Phosphotyrosine1 Publication1
Modified residuei633Phosphotyrosine1 Publication1
Modified residuei671Phosphotyrosine1 Publication1

Post-translational modificationi

Phosphorylated in response to FGFR1 signaling, but is not a direct substrate of FGFR1 or SRC. A mutant where the Tyr phosphorylation sites have been replaced by Phe displays constitutive FGFR1-dependent activation of downstream MAP kinases.1 Publication
N-glycosylated.2 Publications
Proteolytic cleavage in the juxtamembrane region gives rise to a soluble ectodomain.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ6RKD8
PRIDEiQ6RKD8

PTM databases

iPTMnetiQ6RKD8
PhosphoSitePlusiQ6RKD8

Expressioni

Tissue specificityi

Detected in brain (at protein level).1 Publication

Developmental stagei

Detected at comparable levels in embryonic brain and in brain from ten day old animals (at protein level) (PubMed:16872596). Detected in neuroectoderm at 7.5 dpc. Detected in midbrain between 8.5 and 9 dpc (PubMed:18448090). Detected at 9.5 dpc in embryonic midbrain adjacent to the boundary between midbrain and forebrain (PubMed:16872596). At 10.5 dpc, expression is also detected in the eye, throughout the brain, the dorsal root ganglia and trigeminal ganglia, and in cells adjacent to the urogenital ridge in the torso (PubMed:16872596, PubMed:18448090). At 11 dpc, expression in midbrain is tightly restricted to the boundary between midbrain and hindbrain (PubMed:16872596).1 Publication

Inductioni

Up-regulated by FGF2.1 Publication

Gene expression databases

BgeeiENSMUSG00000047787 Expressed in 172 organ(s), highest expression level in olfactory epithelium
GenevisibleiQ6RKD8 MM

Interactioni

Subunit structurei

Interacts with FGFR1 (PubMed:16872596). Interacts (via extracellular domain) with ADGRL1/LPHN1 and ADGRL3 (via olfactomedin-like domain) (PubMed:22405201).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109010

Structurei

3D structure databases

ProteinModelPortaliQ6RKD8
SMRiQ6RKD8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 80LRRNTSequence analysisAdd BLAST27
Repeati81 – 105LRR 1Sequence analysisAdd BLAST25
Repeati106 – 126LRR 2Sequence analysisAdd BLAST21
Repeati127 – 149LRR 3Sequence analysisAdd BLAST23
Repeati151 – 175LRR 4Sequence analysisAdd BLAST25
Repeati176 – 197LRR 5Sequence analysisAdd BLAST22
Repeati198 – 220LRR 6Sequence analysisAdd BLAST23
Repeati222 – 246LRR 7Sequence analysisAdd BLAST25
Repeati247 – 269LRR 8Sequence analysisAdd BLAST23
Repeati270 – 292LRR 9Sequence analysisAdd BLAST23
Repeati293 – 316LRR 10Sequence analysisAdd BLAST24
Domaini328 – 379LRRCTSequence analysisAdd BLAST52
Domaini437 – 532Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST96

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIEA Eukaryota
COG4886 LUCA
GeneTreeiENSGT00920000148996
HOGENOMiHOG000290188
HOVERGENiHBG051629
InParanoidiQ6RKD8
KOiK16362
OMAiSWLRLGH
OrthoDBiEOG091G05YU
PhylomeDBiQ6RKD8
TreeFamiTF331598

Family and domain databases

CDDicd00063 FN3, 1 hit
Gene3Di2.60.40.10, 1 hit
3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR000483 Cys-rich_flank_reg_C
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR000372 LRRNT
PfamiView protein in Pfam
PF13855 LRR_8, 3 hits
PF01462 LRRNT, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 8 hits
SM00082 LRRCT, 1 hit
SM00013 LRRNT, 1 hit
SUPFAMiSSF49265 SSF49265, 1 hit
PROSITEiView protein in PROSITE
PS50853 FN3, 1 hit
PS51450 LRR, 8 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6RKD8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVVAHSAATA TTTPAATVTA TVVMTTATMD LRDWLFLCYG LIAFLTEVID
60 70 80 90 100
STTCPSVCRC DNGFIYCNDR GLTSIPSDIP DDATTLYLQN NQINNAGIPQ
110 120 130 140 150
DLKTKVKVQV IYLYENDLDE FPINLPRSLR ELHLQDNNVR TIARDSLARI
160 170 180 190 200
PLLEKLHLDD NSVSTVSIEE DAFADSKQLK LLFLSRNHLS SIPSGLPHTL
210 220 230 240 250
EELRLDDNRI STIPLHAFKG LNSLRRLVLD GNLLANQRIA DDTFSRLQNL
260 270 280 290 300
TELSLVRNSL AAPPLNLPSA HLQKLYLQDN AISHIPYNTL AKMRELERLD
310 320 330 340 350
LSNNNLTTLP RGLFDDLGNL AQLLLRNNPW FCGCNLMWLR DWVRARAAVV
360 370 380 390 400
NVRGLMCQGP EKVRGMAIKD ITSEMDECFE AGSQGGAANA AAKTTVSNHA
410 420 430 440 450
SATTPQGSLF TLKAKRPGLR LPDSNIDYPM ATGDGAKTLV IQVKPLTADS
460 470 480 490 500
IRITWKAMLP ASSFRLSWLR LGHSPAVGSI TETLVQGDKT EYLLTALEPK
510 520 530 540 550
STYIICMVTM ETGNTYVADE TPVCAKAETA DSYGPTTTLN QEQNAGPMAG
560 570 580 590 600
LPLAGIIGGA VALVFLFLVL GAICWYVHRA GELLTRERVY NRGSRRKDDY
610 620 630 640 650
MESGTKKDNS ILEIRGPGLQ MLPINPYRSK EEYVVHTIFP SNGSSLCKGA
660 670
HTIGYGTTRG YREAGIPDVD YSYT
Length:674
Mass (Da):74,151
Last modified:July 5, 2004 - v1
Checksum:i7C5FDC1E437CF8C5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti481T → I in AAI11880 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY495667 mRNA Translation: AAR92200.1
AY495668 mRNA Translation: AAR92201.1
AK031503 mRNA Translation: BAE20477.1
AC109619 Genomic DNA No translation available.
CH466612 Genomic DNA Translation: EDL33290.1
BC070403 mRNA Translation: AAH70403.1
BC111879 mRNA Translation: AAI11880.1
BC112383 mRNA Translation: AAI12384.1
BC138214 mRNA Translation: AAI38215.1
CCDSiCCDS29519.1
RefSeqiNP_958813.1, NM_201411.2
XP_006527232.1, XM_006527169.2
XP_006527233.1, XM_006527170.3
XP_006527234.1, XM_006527171.3
UniGeneiMm.386930

Genome annotation databases

EnsembliENSMUST00000113383; ENSMUSP00000109010; ENSMUSG00000047787
GeneIDi396184
KEGGimmu:396184
UCSCiuc008gkh.3 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY495667 mRNA Translation: AAR92200.1
AY495668 mRNA Translation: AAR92201.1
AK031503 mRNA Translation: BAE20477.1
AC109619 Genomic DNA No translation available.
CH466612 Genomic DNA Translation: EDL33290.1
BC070403 mRNA Translation: AAH70403.1
BC111879 mRNA Translation: AAI11880.1
BC112383 mRNA Translation: AAI12384.1
BC138214 mRNA Translation: AAI38215.1
CCDSiCCDS29519.1
RefSeqiNP_958813.1, NM_201411.2
XP_006527232.1, XM_006527169.2
XP_006527233.1, XM_006527170.3
XP_006527234.1, XM_006527171.3
UniGeneiMm.386930

3D structure databases

ProteinModelPortaliQ6RKD8
SMRiQ6RKD8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109010

PTM databases

iPTMnetiQ6RKD8
PhosphoSitePlusiQ6RKD8

Proteomic databases

PaxDbiQ6RKD8
PRIDEiQ6RKD8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113383; ENSMUSP00000109010; ENSMUSG00000047787
GeneIDi396184
KEGGimmu:396184
UCSCiuc008gkh.3 mouse

Organism-specific databases

CTDi23769
MGIiMGI:3026647 Flrt1

Phylogenomic databases

eggNOGiENOG410IIEA Eukaryota
COG4886 LUCA
GeneTreeiENSGT00920000148996
HOGENOMiHOG000290188
HOVERGENiHBG051629
InParanoidiQ6RKD8
KOiK16362
OMAiSWLRLGH
OrthoDBiEOG091G05YU
PhylomeDBiQ6RKD8
TreeFamiTF331598

Enzyme and pathway databases

ReactomeiR-MMU-5654687 Downstream signaling of activated FGFR1

Miscellaneous databases

PROiPR:Q6RKD8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047787 Expressed in 172 organ(s), highest expression level in olfactory epithelium
GenevisibleiQ6RKD8 MM

Family and domain databases

CDDicd00063 FN3, 1 hit
Gene3Di2.60.40.10, 1 hit
3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR000483 Cys-rich_flank_reg_C
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR000372 LRRNT
PfamiView protein in Pfam
PF13855 LRR_8, 3 hits
PF01462 LRRNT, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 8 hits
SM00082 LRRCT, 1 hit
SM00013 LRRNT, 1 hit
SUPFAMiSSF49265 SSF49265, 1 hit
PROSITEiView protein in PROSITE
PS50853 FN3, 1 hit
PS51450 LRR, 8 hits
ProtoNetiSearch...

Entry informationi

Entry nameiFLRT1_MOUSE
AccessioniPrimary (citable) accession number: Q6RKD8
Secondary accession number(s): Q14DT7, Q6RKD9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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