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Protein

Deoxyhypusine synthase

Gene

DHS

Organism
Brassica napus (Rape)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Also able to produce homospermidine from putrescine (By similarity).By similarity

Catalytic activityi

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactori

NAD+By similarity

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei136NADBy similarity1
Binding sitei237NADBy similarity1
Binding sitei242SpermidineBy similarity1
Binding sitei284NAD; via amide nitrogenBy similarity1
Binding sitei289SpermidineBy similarity1
Active sitei330NucleophileBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi104 – 108NADBy similarity5
Nucleotide bindingi130 – 132NADBy similarity3
Nucleotide bindingi309 – 310NADBy similarity2
Nucleotide bindingi343 – 344NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processHypusine biosynthesis
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00354

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine synthase (EC:2.5.1.46)
Gene namesi
Name:DHS
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001344741 – 368Deoxyhypusine synthaseAdd BLAST368

Proteomic databases

PRIDEiQ6RJS2

Structurei

3D structure databases

ProteinModelPortaliQ6RJS2
SMRiQ6RJS2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni135 – 136Spermidine bindingBy similarity2
Regioni315 – 317Spermidine bindingBy similarity3
Regioni324 – 330Spermidine bindingBy similarity7

Sequence similaritiesi

Belongs to the deoxyhypusine synthase family.Curated

Family and domain databases

Gene3Di3.40.910.10, 1 hit
InterProiView protein in InterPro
IPR002773 Deoxyhypusine_synthase
IPR036982 Deoxyhypusine_synthase_sf
IPR029035 DHS-like_NAD/FAD-binding_dom
PANTHERiPTHR11703 PTHR11703, 1 hit
PfamiView protein in Pfam
PF01916 DS, 1 hit
SUPFAMiSSF52467 SSF52467, 1 hit
TIGRFAMsiTIGR00321 dhys, 1 hit

Sequencei

Sequence statusi: Complete.

Q6RJS2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEDRVLSSV HSTVFKESES LEGKCDKIEG YDFNQGVNYP KLLRSMLTTG
60 70 80 90 100
FQASNLGDVI DVVNQMLEWR LSDETIAPED CSEEEKDPAY RESVKCKIFL
110 120 130 140 150
GFTSNLVSSG VRETIRYLVQ HHMVDVIVTT TGGVEEDLIK CLAPTFKGDF
160 170 180 190 200
SLPGAYLRSK GLNRIGNLLV PNDNYCKFED WIIPIFDQML KEQKEESVLW
210 220 230 240 250
TPSKLLARLG KEINNESSYL YWAYKMNIPV FCRGLTDGSL GDMLYFHSFR
260 270 280 290 300
TSGLVIDVVQ DIRAMNGEAV HATPRKTGMI ILGGGLPKHH ICNANMMRNG
310 320 330 340 350
ADYAVFINTG QEFDGSDSGA RPDEAVSWGK IRGSAKTVKV YCDATIAFPL
360
LVAETFASKR EQSCEHKT
Length:368
Mass (Da):41,162
Last modified:July 5, 2004 - v1
Checksum:iE8B62D427FF6C51F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY496434 mRNA Translation: AAR91928.1

Similar proteinsi

Entry informationi

Entry nameiDHYS_BRANA
AccessioniPrimary (citable) accession number: Q6RJS2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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