UniProtKB - Q6RFH5 (WDR74_HUMAN)
WD repeat-containing protein 74
WDR74
Functioni
GO - Biological processi
- blastocyst formation Source: UniProtKB
- ribosomal large subunit biogenesis Source: UniProtKB
- RNA metabolic process Source: UniProtKB
- rRNA processing Source: UniProtKB
Enzyme and pathway databases
PathwayCommonsi | Q6RFH5 |
SignaLinki | Q6RFH5 |
Names & Taxonomyi
Protein namesi | Recommended name: WD repeat-containing protein 74Alternative name(s): NOP seven-associated protein 1 |
Gene namesi | Name:WDR74 Synonyms:NSA1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25529, WDR74 |
MIMi | 617947, gene |
neXtProti | NX_Q6RFH5 |
VEuPathDBi | HostDB:ENSG00000133316.15 |
Subcellular locationi
Nucleus
- nucleolus Source: UniProtKB
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- preribosome, large subunit precursor Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 171 – 176 | KNVRND → GSGS: Reduces interaction with NVL. 1 Publication | 6 | |
Mutagenesisi | 185 | W → A: Reduces interaction with NVL. 1 Publication | 1 | |
Mutagenesisi | 191 | F → A: Reduces interaction with NVL. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 54663 |
OpenTargetsi | ENSG00000133316 |
PharmGKBi | PA142670578 |
Miscellaneous databases
Pharosi | Q6RFH5, Tbio |
Genetic variation databases
BioMutai | WDR74 |
DMDMi | 55976441 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051428 | 1 – 385 | WD repeat-containing protein 74Add BLAST | 385 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 214 | PhosphoserineCombined sources | 1 | |
Modified residuei | 311 | N6-methyllysineCombined sources | 1 | |
Modified residuei | 361 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | Q6RFH5 |
jPOSTi | Q6RFH5 |
MassIVEi | Q6RFH5 |
MaxQBi | Q6RFH5 |
PaxDbi | Q6RFH5 |
PeptideAtlasi | Q6RFH5 |
PRIDEi | Q6RFH5 |
ProteomicsDBi | 67316 [Q6RFH5-1] 67317 [Q6RFH5-2] |
2D gel databases
SWISS-2DPAGEi | Q6RFH5 |
PTM databases
iPTMneti | Q6RFH5 |
PhosphoSitePlusi | Q6RFH5 |
SwissPalmi | Q6RFH5 |
Expressioni
Gene expression databases
Bgeei | ENSG00000133316, Expressed in calcaneal tendon and 226 other tissues |
ExpressionAtlasi | Q6RFH5, baseline and differential |
Genevisiblei | Q6RFH5, HS |
Organism-specific databases
HPAi | ENSG00000133316, Tissue enhanced (bone) |
Interactioni
Subunit structurei
Isoform 1 interacts (through WDR repeats) with NVL; the interaction is independent of RNA or pre-60S ribosome particles. Isoform 2 does not interact with NVL (PubMed:28416111).
Interacts with MTREX; the interaction dissociation in a late stage of rRNA synthesis is required for appropriate maturation of pre-60S particles and depends on the ATPase activity of NVL (PubMed:26456651, PubMed:29107693).
3 PublicationsBinary interactionsi
Q6RFH5
With | #Exp. | IntAct |
---|---|---|
RCHY1 [Q96PM5] | 3 | EBI-722366,EBI-947779 |
Protein-protein interaction databases
BioGRIDi | 120092, 58 interactors |
IntActi | Q6RFH5, 29 interactors |
MINTi | Q6RFH5 |
STRINGi | 9606.ENSP00000432119 |
Miscellaneous databases
RNActi | Q6RFH5, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 40 – 80 | WD 1Add BLAST | 41 | |
Repeati | 83 – 122 | WD 2Add BLAST | 40 | |
Repeati | 128 – 168 | WD 3Add BLAST | 41 | |
Repeati | 179 – 220 | WD 4Add BLAST | 42 | |
Repeati | 224 – 266 | WD 5Add BLAST | 43 | |
Repeati | 267 – 306 | WD 6Add BLAST | 40 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 320 – 385 | Required for nucleolar and nuclear location1 PublicationAdd BLAST | 66 |
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG3881, Eukaryota |
GeneTreei | ENSGT00390000015119 |
HOGENOMi | CLU_033769_2_0_1 |
InParanoidi | Q6RFH5 |
OMAi | SRLCSVW |
OrthoDBi | 1439033at2759 |
PhylomeDBi | Q6RFH5 |
TreeFami | TF314666 |
Family and domain databases
Gene3Di | 2.130.10.10, 2 hits |
InterProi | View protein in InterPro IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR036322, WD40_repeat_dom_sf IPR037379, WDR74/Nsa1 |
PANTHERi | PTHR16038, PTHR16038, 1 hit |
Pfami | View protein in Pfam PF00400, WD40, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 3 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAAAARWNH VWVGTETGIL KGVNLQRKQA ANFTAGGQPR REEAVSALCW
60 70 80 90 100
GTGGETQMLV GCADRTVKHF STEDGIFQGQ RHCPGGEGMF RGLAQADGTL
110 120 130 140 150
ITCVDSGILR VWHDKDKDTS SDPLLELRVG PGVCRMRQDP AHPHVVATGG
160 170 180 190 200
KENALKIWDL QGSEEPVFRA KNVRNDWLDL RVPIWDQDIQ FLPGSQKLVT
210 220 230 240 250
CTGYHQVRVY DPASPQRRPV LETTYGEYPL TAMTLTPGGN SVIVGNTHGQ
260 270 280 290 300
LAEIDLRQGR LLGCLKGLAG SVRGLQCHPS KPLLASCGLD RVLRIHRIQN
310 320 330 340 350
PRGLEHKVYL KSQLNCLLLS GRDNWEDEPQ EPQEPNKVPL EDTETDELWA
360 370 380
SLEAAAKRKL SGLEQPQGAL QTRRRKKKRP GSTSP
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PS41 | E9PS41_HUMAN | WD repeat-containing protein 74 | WDR74 | 328 | Annotation score: | ||
H0YFP2 | H0YFP2_HUMAN | WD repeat-containing protein 74 | WDR74 | 38 | Annotation score: | ||
F5GYL6 | F5GYL6_HUMAN | WD repeat-containing protein 74 | WDR74 | 57 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 33 | F → L in BAA91610 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 229 | P → S in BAA91610 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 297 – 298 | RI → GT in BAA91610 (PubMed:14702039).Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011957 | 308 – 326 | Missing in isoform 2. 1 PublicationAdd BLAST | 19 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY500828 mRNA Translation: AAS48499.1 AK001301 mRNA Translation: BAA91610.1 AK025383 mRNA Translation: BAB15122.1 AK292330 mRNA Translation: BAF85019.1 BC006351 mRNA Translation: AAH06351.1 |
CCDSi | CCDS44630.1 [Q6RFH5-1] CCDS76421.1 [Q6RFH5-2] |
RefSeqi | NP_001294906.1, NM_001307977.1 [Q6RFH5-2] NP_060563.2, NM_018093.3 [Q6RFH5-1] XP_005274112.1, XM_005274055.2 |
Genome annotation databases
Ensembli | ENST00000278856; ENSP00000278856; ENSG00000133316 [Q6RFH5-1] ENST00000311713; ENSP00000308931; ENSG00000133316 [Q6RFH5-2] ENST00000525239; ENSP00000432119; ENSG00000133316 [Q6RFH5-1] ENST00000529106; ENSP00000435726; ENSG00000133316 [Q6RFH5-1] |
GeneIDi | 54663 |
KEGGi | hsa:54663 |
UCSCi | uc001nvl.3, human [Q6RFH5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY500828 mRNA Translation: AAS48499.1 AK001301 mRNA Translation: BAA91610.1 AK025383 mRNA Translation: BAB15122.1 AK292330 mRNA Translation: BAF85019.1 BC006351 mRNA Translation: AAH06351.1 |
CCDSi | CCDS44630.1 [Q6RFH5-1] CCDS76421.1 [Q6RFH5-2] |
RefSeqi | NP_001294906.1, NM_001307977.1 [Q6RFH5-2] NP_060563.2, NM_018093.3 [Q6RFH5-1] XP_005274112.1, XM_005274055.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 120092, 58 interactors |
IntActi | Q6RFH5, 29 interactors |
MINTi | Q6RFH5 |
STRINGi | 9606.ENSP00000432119 |
PTM databases
iPTMneti | Q6RFH5 |
PhosphoSitePlusi | Q6RFH5 |
SwissPalmi | Q6RFH5 |
Genetic variation databases
BioMutai | WDR74 |
DMDMi | 55976441 |
2D gel databases
SWISS-2DPAGEi | Q6RFH5 |
Proteomic databases
EPDi | Q6RFH5 |
jPOSTi | Q6RFH5 |
MassIVEi | Q6RFH5 |
MaxQBi | Q6RFH5 |
PaxDbi | Q6RFH5 |
PeptideAtlasi | Q6RFH5 |
PRIDEi | Q6RFH5 |
ProteomicsDBi | 67316 [Q6RFH5-1] 67317 [Q6RFH5-2] |
Protocols and materials databases
Antibodypediai | 28811, 187 antibodies |
DNASUi | 54663 |
Genome annotation databases
Ensembli | ENST00000278856; ENSP00000278856; ENSG00000133316 [Q6RFH5-1] ENST00000311713; ENSP00000308931; ENSG00000133316 [Q6RFH5-2] ENST00000525239; ENSP00000432119; ENSG00000133316 [Q6RFH5-1] ENST00000529106; ENSP00000435726; ENSG00000133316 [Q6RFH5-1] |
GeneIDi | 54663 |
KEGGi | hsa:54663 |
UCSCi | uc001nvl.3, human [Q6RFH5-1] |
Organism-specific databases
CTDi | 54663 |
DisGeNETi | 54663 |
GeneCardsi | WDR74 |
HGNCi | HGNC:25529, WDR74 |
HPAi | ENSG00000133316, Tissue enhanced (bone) |
MIMi | 617947, gene |
neXtProti | NX_Q6RFH5 |
OpenTargetsi | ENSG00000133316 |
PharmGKBi | PA142670578 |
VEuPathDBi | HostDB:ENSG00000133316.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3881, Eukaryota |
GeneTreei | ENSGT00390000015119 |
HOGENOMi | CLU_033769_2_0_1 |
InParanoidi | Q6RFH5 |
OMAi | SRLCSVW |
OrthoDBi | 1439033at2759 |
PhylomeDBi | Q6RFH5 |
TreeFami | TF314666 |
Enzyme and pathway databases
PathwayCommonsi | Q6RFH5 |
SignaLinki | Q6RFH5 |
Miscellaneous databases
BioGRID-ORCSi | 54663, 598 hits in 995 CRISPR screens |
ChiTaRSi | WDR74, human |
GenomeRNAii | 54663 |
Pharosi | Q6RFH5, Tbio |
PROi | PR:Q6RFH5 |
RNActi | Q6RFH5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000133316, Expressed in calcaneal tendon and 226 other tissues |
ExpressionAtlasi | Q6RFH5, baseline and differential |
Genevisiblei | Q6RFH5, HS |
Family and domain databases
Gene3Di | 2.130.10.10, 2 hits |
InterProi | View protein in InterPro IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR036322, WD40_repeat_dom_sf IPR037379, WDR74/Nsa1 |
PANTHERi | PTHR16038, PTHR16038, 1 hit |
Pfami | View protein in Pfam PF00400, WD40, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 3 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | WDR74_HUMAN | |
Accessioni | Q6RFH5Primary (citable) accession number: Q6RFH5 Secondary accession number(s): A8K8G5 Q9NVY2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 23, 2004 |
Last sequence update: | July 5, 2004 | |
Last modified: | April 7, 2021 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot