Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 76 (12 Aug 2020)
Sequence version 1 (05 Jul 2004)
Previous versions | rss
Help videoAdd a publicationFeedback
Protein

Non-structural polyprotein pORF1

Gene

ORF1

Organism
Avian hepatitis E virus (isolate Chicken/California/Meng) (AHEV)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. The enzymatic reaction involves a covalent link between 7-methyl-GMP and the methyltransferase, whereas eukaryotic capping enzymes form a covalent complex only with GMP. Methyltransferase catalyzes transfer of a methyl group from S-adenosylmethionine to GTP and GDP to yield m7GTP or m7GDP. GMP, GpppG, and GpppA were poor substrates for the methyltransferase. This enzyme also displays guanylyltransferase activity to form a covalent complex, methyltransferase-m7GMP, from which 7-methyl-GMP is transferred to the mRNA to create the cap structure. Cap analogs such as m7GTP, m7GDP, et2m7GMP, and m2et7GMP inhibit the methyltransferase reaction (By similarity).By similarity
RNA-directed RNA polymerase plays an essential role in the virus replication. Binds to the 3'-end of the genomic RNA, probably to initiate replication (By similarity).PROSITE-ProRule annotation
Helicase region exhibits NTPase and RNA unwinding activities.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi818 – 825ATPSequence analysis8

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHelicase, Hydrolase, Methyltransferase, Nucleotidyltransferase, Protease, RNA-binding, RNA-directed RNA polymerase, Thiol protease, Transferase
Biological processViral RNA replication
LigandATP-binding, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Non-structural polyprotein pORF1
Including the following 4 domains:
Methyltransferase (EC:2.1.1.-, EC:2.7.7.-)
Putative papain-like cysteine protease (EC:3.4.22.-)
Short name:
PLP
NTPase/helicase (EC:3.6.4.-)
RNA-directed RNA polymerase (EC:2.7.7.48)
Short name:
RdRp
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
ORF Names:ORF1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiAvian hepatitis E virus (isolate Chicken/California/Meng) (AHEV)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri516993 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiVirusesRiboviriaOrthornaviraeKitrinoviricotaAlsuviricetesHepeliviralesHepeviridaeOrthohepevirus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiGallus gallus (Chicken) [TaxID: 9031]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000007439 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Genome
  • UP000139094 Componenti: Genome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003345291 – 1531Non-structural polyprotein pORF1Add BLAST1531

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

It is not yet clear whether the ORF1-encoded polyprotein contains multiple biochemical activities, or undergoes cis or trans- processing to release biochemically distinct peptides.

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
Q6QLN1

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini56 – 239Alphavirus-like MTPROSITE-ProRule annotationAdd BLAST184
Domaini612 – 761MacroPROSITE-ProRule annotationAdd BLAST150
Domaini786 – 924(+)RNA virus helicase ATP-bindingAdd BLAST139
Domaini925 – 1058(+)RNA virus helicase C-terminalAdd BLAST134
Domaini1295 – 1406RdRp catalyticPROSITE-ProRule annotationAdd BLAST112

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni60 – 239MethyltransferaseBy similarityAdd BLAST180
Regioni238 – 434Y-domainBy similarityAdd BLAST197
Regioni435 – 621Putative papain-like cysteine proteaseBy similarityAdd BLAST187
Regioni622 – 786X-domainBy similarityAdd BLAST165
Regioni803 – 1046NTPase/helicaseBy similarityAdd BLAST244
Regioni1049 – 1531RNA-directed RNA polymeraseBy similarityAdd BLAST483

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi452 – 469Cys-richAdd BLAST18

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.220.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR027351, (+)RNA_virus_helicase_core_dom
IPR002588, Alphavirus-like_MT_dom
IPR043502, DNA/RNA_pol_sf
IPR022202, Hepatitis-E_hinge
IPR002589, Macro_dom
IPR043472, Macro_dom-like
IPR027417, P-loop_NTPase
IPR007094, RNA-dir_pol_PSvirus
IPR001788, Tymovirus_RNA-dep_RNA_pol

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF12526, DUF3729, 1 hit
PF00978, RdRP_2, 1 hit
PF01443, Viral_helicase1, 1 hit
PF01660, Vmethyltransf, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00506, A1pp, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF52540, SSF52540, 2 hits
SSF52949, SSF52949, 1 hit
SSF56672, SSF56672, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51743, ALPHAVIRUS_MT, 1 hit
PS51154, MACRO, 1 hit
PS51657, PSRV_HELICASE, 1 hit
PS50507, RDRP_SSRNA_POS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q6QLN1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDVSQFAESK GVKTALEAAA LAAANTALRN ARVVTPYLTQ QQTKNLLELF
60 70 80 90 100
RGAQLRFEPR DNWAHPVQRV VHDALEQYVR RAAGPNCLEV GAHPRSINRH
110 120 130 140 150
QASHRCFLPP VGRDEQRWQV APRRGLCNLI RRALLNGVKV AREFCQLGFG
160 170 180 190 200
ACSHQCEVGI ALYSLHDMRP ADVACAMARH NMRTMYVVLH LPEEAMLPPG
210 220 230 240 250
SYSNKFYNTV NTADKCIITY ADDSCAGYVH KREVLQDWIT TTGVSGRHPM
260 270 280 290 300
LIERVRAIGC HFVLLCTATQ PCPMPYTPYP SSNTVYVRNV YGPALGAGLF
310 320 330 340 350
TPKCCVDATF YPVPRRVWQR LMMFGTTLDD DAFCCSRLLT YLRGISTKVT
360 370 380 390 400
VGNIVANEGW QPEEQQLTAV AIAAYLTVCH QRWVRTQGIA RGVRRLQAEH
410 420 430 440 450
AQQFWFKVWE LFTNTGTVPG YSAGFYRQLA TWISGGLTID FERRVFDKRV
460 470 480 490 500
KCGCCCVCER RPADPGCLCI DDFPDGANGL VKLKKWPIRA GTKSAVSKWA
510 520 530 540 550
QVRVRADSTE DLIDLSVPKL LTLKELAAAA IRKQPSAPPS LHILDRRPVG
560 570 580 590 600
DPRRPVNCAP PAVSAGPVPA PPGNPVIESV QGSGAGGPEV SESQPGLTPT
610 620 630 640 650
REVTNMPLPP QRGQEEVLAV LPSGARVIVG NLLDVAADWL VNPANRDHQP
660 670 680 690 700
GGGLCGMFHR RWPHLWPVCG EVQDLPTGPV IFQQGPPKVI HAPGPDYRIK
710 720 730 740 750
PDPDGLRRVY AVVHQAHGTV ASPLISAGIY RAPARESFEA WAATARDGDL
760 770 780 790 800
LVVQRSMAQH IRDFVLNEGR HRPRELHVDR AMADMVNYGL ATEPEPYNEL
810 820 830 840 850
VKGVEVAPMT VKYALIAGVP GSGKSSSVDH RGAVVITPTK TLAREWSARG
860 870 880 890 900
ATAVTPHVAA SAAPEGRVIV DEAYAIPPHL LVASLRRARD VVMLGDPHQI
910 920 930 940 950
PALDFDGRCL TSAVDLGLQP TSWRTVSHRC PWDVCIFLRT DYPTITTTSR
960 970 980 990 1000
VLRSVVFTGE TIGQKIVFTQ VAKQSNPGSI TVHEAQGSTF DQTTIIATLD
1010 1020 1030 1040 1050
ARGLIASSRA HAIVALTRHR ERCSVIDVGG VLVEIGVTDA MFNNIEMQLV
1060 1070 1080 1090 1100
RPDAAAPAGV LRAPDDTVDG LLDIPPAHTD VAAVLTAEAI GHAPLELAAI
1110 1120 1130 1140 1150
NPPGPVLEQG LLYMPARLDG RDEVVKLQLS DTVHCRLAAP TSRLAVINTL
1160 1170 1180 1190 1200
VGRYGKATKL PEVEYDLMDT IAQFWHHIGP INPSTLEYAE MCEAMLSKGQ
1210 1220 1230 1240 1250
DGSLIVHLDL QDADCSRITF FQKDCAKFTL DDPVAHGKVG QGISAWPKTL
1260 1270 1280 1290 1300
CALFGPWFRA IEKHLVAGLP PGYYYGDLYT EADLHRSVLC APAGHLVFEN
1310 1320 1330 1340 1350
DFSEFDSTQN NVSLDLECEL MRRFGMPDWM VALYHLVRSY WLLVAPKEAL
1360 1370 1380 1390 1400
RGCWKKHSGE PGTLLWNTVW NMTVLHHVYE FDRPSVLCFK GDDSVVVCES
1410 1420 1430 1440 1450
VRARPEGVSL VADCGLKMKD KTGPCGAFSN LLIFPGAGVV CDLLRQWGRL
1460 1470 1480 1490 1500
TDKNWGPDIQ RMQDLEQACK DFVARVVTQG KEMLTIQLVA GYYGVEVGMV
1510 1520 1530
EVVWGALKAC AAARETLVTN RLPVLNLSKE D
Length:1,531
Mass (Da):168,040
Last modified:July 5, 2004 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i730F80BA135CEA95
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AY535004 Genomic RNA Translation: AAS45830.1
AY043166 Genomic RNA Translation: AAL13366.1

NCBI Reference Sequences

More...
RefSeqi
YP_009001465.1, NC_023425.1

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
18263429

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
vg:18263429

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY535004 Genomic RNA Translation: AAS45830.1
AY043166 Genomic RNA Translation: AAL13366.1
RefSeqiYP_009001465.1, NC_023425.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Proteomic databases

PRIDEiQ6QLN1

Genome annotation databases

GeneIDi18263429
KEGGivg:18263429

Family and domain databases

Gene3Di3.40.220.10, 1 hit
InterProiView protein in InterPro
IPR027351, (+)RNA_virus_helicase_core_dom
IPR002588, Alphavirus-like_MT_dom
IPR043502, DNA/RNA_pol_sf
IPR022202, Hepatitis-E_hinge
IPR002589, Macro_dom
IPR043472, Macro_dom-like
IPR027417, P-loop_NTPase
IPR007094, RNA-dir_pol_PSvirus
IPR001788, Tymovirus_RNA-dep_RNA_pol
PfamiView protein in Pfam
PF12526, DUF3729, 1 hit
PF00978, RdRP_2, 1 hit
PF01443, Viral_helicase1, 1 hit
PF01660, Vmethyltransf, 1 hit
SMARTiView protein in SMART
SM00506, A1pp, 1 hit
SUPFAMiSSF52540, SSF52540, 2 hits
SSF52949, SSF52949, 1 hit
SSF56672, SSF56672, 1 hit
PROSITEiView protein in PROSITE
PS51743, ALPHAVIRUS_MT, 1 hit
PS51154, MACRO, 1 hit
PS51657, PSRV_HELICASE, 1 hit
PS50507, RDRP_SSRNA_POS, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPOLN_AHEV
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q6QLN1
Secondary accession number(s): Q913Y9
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 5, 2004
Last modified: August 12, 2020
This is version 76 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again