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Protein

Cyclic AMP-responsive element-binding protein 3-like protein 2

Gene

Creb3l2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Transcription factor involved in unfolded protein response (UPR). In the absence of endoplasmic reticulum (ER) stress, inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. In response to ER stress, transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. Plays a critical role in chondrogenesis by activating the transcription of SEC23A, which promotes the transport and secretion of cartilage matrix proteins, and possibly that of ER biogenesis-related genes. In a neuroblastoma cell line, protects cells from ER stress-induced death. In vitro activates transcription of target genes via direct binding to the CRE site.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processTranscription, Transcription regulation, Unfolded protein response

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic AMP-responsive element-binding protein 3-like protein 2
Short name:
cAMP-responsive element-binding protein 3-like protein 2
Alternative name(s):
SCI-related protein Ra69
Cleaved into the following chain:
Gene namesi
Name:Creb3l2
Synonyms:Ra69
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi1306040 Creb3l2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 378CytoplasmicSequence analysisAdd BLAST378
Transmembranei379 – 399Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini400 – 521LumenalSequence analysisAdd BLAST122

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002880701 – 521Cyclic AMP-responsive element-binding protein 3-like protein 2Add BLAST521
ChainiPRO_00002962121 – ?Processed cyclic AMP-responsive element-binding protein 3-like protein 2

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei93PhosphoserineBy similarity1
Cross-linki178Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei191PhosphoserineBy similarity1
Glycosylationi505N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi518N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Upon ER stress, translocated to the Golgi apparatus, where it is processed by regulated intramembrane proteolysis (RIP) to release the cytosol-facing N-terminal transcription factor domain. The cleavage is performed sequentially by site-1 and site-2 proteases (S1P/MBTPS1 and S2P/MBTPS2).By similarity
N-glycosylated.By similarity
Ubiquitinated by HRD1/SYVN1; undergoes 'Lys-48'-linked ubiquitination, followed by rapid proteasomal degradation under normal conditions. Upon ER stress, SYVN1 E3 ubiquitin-protein ligase dissociates from its substrate, ubiquitination does not occur and CREB3L2 is stabilized.By similarity

Keywords - PTMi

Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6QDP7

Expressioni

Gene expression databases

BgeeiENSRNOG00000012826
GenevisibleiQ6QDP7 RN

Interactioni

Subunit structurei

Binds DNA as a dimer.By similarity

Protein-protein interaction databases

BioGridi263374, 2 interactors
STRINGi10116.ENSRNOP00000017338

Structurei

3D structure databases

ProteinModelPortaliQ6QDP7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini294 – 357bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni296 – 325Basic motifPROSITE-ProRule annotationAdd BLAST30
Regioni336 – 357Leucine-zipperPROSITE-ProRule annotationAdd BLAST22

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi427 – 430S1P recognitionBy similarity4

Sequence similaritiesi

Belongs to the bZIP family. ATF subfamily.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0709 Eukaryota
ENOG410ZZQM LUCA
GeneTreeiENSGT00520000055538
HOGENOMiHOG000060150
HOVERGENiHBG057480
InParanoidiQ6QDP7
KOiK09048
OMAiNLLIYEE
OrthoDBiEOG091G081Z
PhylomeDBiQ6QDP7
TreeFamiTF316079

Family and domain databases

InterProiView protein in InterPro
IPR029804 BBF2H7
IPR004827 bZIP
PANTHERiPTHR22952:SF96 PTHR22952:SF96, 1 hit
PfamiView protein in Pfam
PF00170 bZIP_1, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
PS00036 BZIP_BASIC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6QDP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVLESGEQS VLQWDRKLSE LSEPGETEAL MYHTHFSELL DEFSQNVLGQ
60 70 80 90 100
LLNDPFLSEK SVSMEVEPSP TSPAPLIQAE HSYSLSEEPR AQSPFTHVAA
110 120 130 140 150
SDGFNDEEVE SEKWYLSTEF PSATIKTEPI TEEQPPGLVP SVTLTITAIS
160 170 180 190 200
TPFEKEESPL DMSAGGDSSC QTLIPKIKLE PHEVDQFLNF SPKEASVDQL
210 220 230 240 250
HLPPTPPSSH SSDSEGSLSP NPRLHPFSLS QAHSPGRAMP RGPSALSTSP
260 270 280 290 300
LLTAPHKLQG SGPLVLTEEE KRTLIAEGYP IPTKLPLTKS EEKALKKIRR
310 320 330 340 350
KIKNKISAQE SRRKKKEYMD SLEKKVESCS TENLELRKKV EVLENTNRTL
360 370 380 390 400
LQQLQKLQTL VMGKVSRTCK LAGTQTGTCL MVVVLCFAVA FGSLFQGYGL
410 420 430 440 450
YPSATKMALP SQHPLSEPYT ASVVRSRNLL IYEEHSSLEE SSSPASAGEL
460 470 480 490 500
GGWDRGSSLL RASSGLEALP EVDLPHFIIS KETSLEKSVL LELQQHLVSS
510 520
KLEGNETLKV VELERRVNAT F
Length:521
Mass (Da):57,379
Last modified:July 5, 2004 - v1
Checksum:iE3949549518855E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY546001 mRNA Translation: AAS49162.1
RefSeqiNP_001012188.1, NM_001012188.1
UniGeneiRn.23031

Genome annotation databases

EnsembliENSRNOT00000017338; ENSRNOP00000017338; ENSRNOG00000012826
GeneIDi362339
KEGGirno:362339
UCSCiRGD:1306040 rat

Similar proteinsi

Entry informationi

Entry nameiCR3L2_RAT
AccessioniPrimary (citable) accession number: Q6QDP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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