UniProtKB - Q6QBQ4 (PLS3_RAT)
Protein
Phospholipid scramblase 3
Gene
Plscr3
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes calcium-induced ATP-independent rapid bidirectional and non-specific movement of the phospholipids (lipid scrambling or lipid flip-flop) between the inner and outer membrane of the mitochondria (PubMed:29171872). Plays an important role in mitochondrial respiratory function, morphology, and apoptotic response (By similarity). Mediates the translocation of cardiolipin from the mitochondrial inner membrane to outer membrane enhancing t-Bid induced cytochrome c release and apoptosis (By similarity). Enhances TNFSF10-induced apoptosis by regulating the distribution of cardiolipin in the mitochondrial membrane resulting in increased release of apoptogenic factors and consequent amplification of the activity of caspases (By similarity). Regulates cardiolipin de novo biosynthesis and its resynthesis (By similarity).By similarity1 Publication
Catalytic activityi
- This reaction proceeds in the forwardBy similarity direction.
- a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)1 PublicationThis reaction proceeds in the forward1 Publication direction.
- a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)By similarity
- a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol)(in) = 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol)(out)By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
pH dependencei
Optimum pH is 5.8. Activity decreases at higher pH values.1 Publication
GO - Molecular functioni
- calcium-dependent protein binding Source: RGD
- calcium ion binding Source: UniProtKB
- lead ion binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- mercury ion binding Source: UniProtKB
- phospholipid scramblase activity Source: UniProtKB
- protein self-association Source: UniProtKB
- SH3 domain binding Source: UniProtKB-KW
GO - Biological processi
- apoptotic process Source: RGD
- cardiolipin biosynthetic process Source: UniProtKB
- cellular response to lipopolysaccharide Source: RGD
- cholesterol homeostasis Source: RGD
- glucose homeostasis Source: RGD
- plasma membrane phospholipid scrambling Source: GO_Central
- regulation of apoptotic process Source: UniProtKB
- regulation of release of cytochrome c from mitochondria Source: UniProtKB
Keywordsi
Biological process | Apoptosis |
Ligand | Calcium |
Names & Taxonomyi
Protein namesi | Recommended name: Phospholipid scramblase 3Short name: PL scramblase 3 Alternative name(s): Ca(2+)-dependent phospholipid scramblase 3 |
Gene namesi | Name:Plscr3 Synonyms:Pls3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1307016, Plscr3 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Mitochondrion
- Mitochondrion inner membrane 1 Publication; Single-pass type II membrane protein 1 Publication
Note: Palmitoylation regulates its localization to the cell membrane or the nucleus; trafficking to the cell membrane is dependent upon palmitoylation whereas in the absence of palmitoylation, localizes to the nucleus.By similarity
Cytosol
- cytosol Source: RGD
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB
- mitochondrion Source: RGD
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: RGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 266 | Mitochondrial intermembrane1 PublicationAdd BLAST | 266 | |
Transmembranei | 267 – 283 | HelicalSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100791 | 1 – 296 | Phospholipid scramblase 3Add BLAST | 296 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 159 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 161 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 163 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 164 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 166 | S-palmitoyl cysteineBy similarity | 1 |
Post-translational modificationi
Palmitoylation regulates its localization to the cell membrane or the nucleus; trafficking to the cell membrane is dependent upon palmitoylation whereas in the absence of palmitoylation, localizes to the nucleus.By similarity
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
PaxDbi | Q6QBQ4 |
PRIDEi | Q6QBQ4 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000027914, Expressed in lung and 20 other tissues |
Genevisiblei | Q6QBQ4, RN |
Interactioni
Subunit structurei
Monomer (By similarity). Forms homooligomers upon binding to Ca2+, Pb2+ and Hg2+ ions (By similarity).
Interacts with PDCD6 in a calcium-dependent manner (By similarity).
Interacts with PRKCD; interaction is enhanced by UV irradiation (By similarity).
By similarityGO - Molecular functioni
- calcium-dependent protein binding Source: RGD
- protein self-association Source: UniProtKB
- SH3 domain binding Source: UniProtKB-KW
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000035566 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 58 | Proline-rich domain (PRD)By similarityAdd BLAST | 58 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 7 – 15 | SH3-binding 1Sequence analysis | 9 | |
Motifi | 15 – 18 | PPxY motifSequence analysis | 4 | |
Motifi | 21 – 27 | SH3-binding 2Sequence analysis | 7 | |
Motifi | 66 – 71 | SH3-binding 3Sequence analysis | 6 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 159 – 167 | Cys-rich | 9 |
Domaini
The Proline-rich domain is required for phospholipid scramblase activity.By similarity
Sequence similaritiesi
Belongs to the phospholipid scramblase family.Curated
Keywords - Domaini
Repeat, SH3-binding, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0621, Eukaryota |
GeneTreei | ENSGT00940000161755 |
HOGENOMi | CLU_053024_1_0_1 |
InParanoidi | Q6QBQ4 |
OMAi | ESGCCER |
OrthoDBi | 1015148at2759 |
PhylomeDBi | Q6QBQ4 |
TreeFami | TF314939 |
Family and domain databases
InterProi | View protein in InterPro IPR005552, Scramblase |
PANTHERi | PTHR23248, PTHR23248, 1 hit |
Pfami | View protein in Pfam PF03803, Scramblase, 1 hit |
i Sequence
Sequence statusi: Complete.
Q6QBQ4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGYLPPKGY APSPPPPYPV PAGYPEAAAL HPGPGQAPVP TQGPAPAPGF
60 70 80 90 100
SLFPSPGPVV PGPPGPFVPL PGVPSGLEFL VQIDQILIHQ KAERVETFLG
110 120 130 140 150
WETCNRYELR SGTGQQLGQA AEESNCCARL CCGARRPLRI RLADPGDREV
160 170 180 190 200
LRLLRPLHCG CSCCPCGLQE MEVQAPPGTT IGHVLQTWHP FIPKFSILDA
210 220 230 240 250
DRQPVLRVVG PCCTCGCGTD TNFEVKTKDE SRSVGRISKQ WGGLLREALT
260 270 280 290
DADDFGLQFP VDLDVRVKAV LLGATFLIDY MFFEKRGGAG PSAITS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY548831 mRNA Translation: AAS55061.1 BC098055 mRNA Translation: AAH98055.1 |
RefSeqi | NP_001012139.1, NM_001012139.2 |
Genome annotation databases
Ensembli | ENSRNOT00000031640; ENSRNOP00000035566; ENSRNOG00000027914 |
GeneIDi | 360549 |
KEGGi | rno:360549 |
UCSCi | RGD:1307016, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY548831 mRNA Translation: AAS55061.1 BC098055 mRNA Translation: AAH98055.1 |
RefSeqi | NP_001012139.1, NM_001012139.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000035566 |
Proteomic databases
PaxDbi | Q6QBQ4 |
PRIDEi | Q6QBQ4 |
Genome annotation databases
Ensembli | ENSRNOT00000031640; ENSRNOP00000035566; ENSRNOG00000027914 |
GeneIDi | 360549 |
KEGGi | rno:360549 |
UCSCi | RGD:1307016, rat |
Organism-specific databases
CTDi | 57048 |
RGDi | 1307016, Plscr3 |
Phylogenomic databases
eggNOGi | KOG0621, Eukaryota |
GeneTreei | ENSGT00940000161755 |
HOGENOMi | CLU_053024_1_0_1 |
InParanoidi | Q6QBQ4 |
OMAi | ESGCCER |
OrthoDBi | 1015148at2759 |
PhylomeDBi | Q6QBQ4 |
TreeFami | TF314939 |
Miscellaneous databases
ChiTaRSi | Plscr3, rat |
PROi | PR:Q6QBQ4 |
Gene expression databases
Bgeei | ENSRNOG00000027914, Expressed in lung and 20 other tissues |
Genevisiblei | Q6QBQ4, RN |
Family and domain databases
InterProi | View protein in InterPro IPR005552, Scramblase |
PANTHERi | PTHR23248, PTHR23248, 1 hit |
Pfami | View protein in Pfam PF03803, Scramblase, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PLS3_RAT | |
Accessioni | Q6QBQ4Primary (citable) accession number: Q6QBQ4 Secondary accession number(s): Q4V7A3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2005 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 98 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families