UniProtKB - Q6Q0N1 (CNDP2_RAT)
Cytosolic non-specific dipeptidase
Cndp2
Functioni
Catalyzes the peptide bond hydrolysis in dipeptides, displaying a non-redundant activity toward threonyl dipeptides. Mediates threonyl dipeptide catabolism in a tissue-specific way (By similarity).
Has high dipeptidase activity toward cysteinylglycine, an intermediate metabolite in glutathione metabolism. Metabolizes N-lactoyl-amino acids, both through hydrolysis to form lactic acid and amino acids, as well as through their formation by reverse proteolysis. Plays a role in the regulation of cell cycle arrest and apoptosis (By similarity).
By similarityCatalytic activityi
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- Hydrolysis of dipeptides, preferentially hydrophobic dipeptides including prolyl amino acids.By similarity EC:3.4.13.18
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 99 | Manganese 2; via tele nitrogenBy similarity | 1 | |
Active sitei | 101 | By similarity | 1 | |
Metal bindingi | 132 | Manganese 1By similarity | 1 | |
Metal bindingi | 132 | Manganese 2By similarity | 1 | |
Active sitei | 166 | Proton acceptorBy similarity | 1 | |
Metal bindingi | 167 | Manganese 1By similarity | 1 | |
Metal bindingi | 195 | Manganese 2By similarity | 1 | |
Binding sitei | 195 | Substrate; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 228 | Substrate; shared with homodimeric partnerBy similarity | 1 | |
Sitei | 228 | Important for catalytic activityBy similarity | 1 | |
Binding sitei | 330 | Substrate; shared with homodimeric partnerBy similarity | 1 | |
Binding sitei | 343 | SubstrateBy similarity | 1 | |
Binding sitei | 417 | Substrate; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 445 | Manganese 1; via tele nitrogenBy similarity | 1 | |
Binding sitei | 445 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- alanylglutamate dipeptidase activity Source: UniProtKB-EC
- carboxypeptidase activity Source: UniProtKB-KW
- dipeptidase activity Source: RGD
- metal ion binding Source: UniProtKB-KW
- metallodipeptidase activity Source: InterPro
- peptidase activity Source: RGD
GO - Biological processi
- proteolysis Source: RGD
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Metalloprotease, Protease |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
Reactomei | R-RNO-174403, Glutathione synthesis and recycling |
Protein family/group databases
MEROPSi | M20.005 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosolic non-specific dipeptidase (EC:3.4.13.18By similarity)Alternative name(s): CNDP dipeptidase 2 Threonyl dipeptidaseBy similarity |
Gene namesi | Name:Cndp2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1305827, Cndp2 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytosol
- cytosol Source: GO_Central
Nucleus
- nucleoplasm Source: Ensembl
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000288496 | 1 – 475 | Cytosolic non-specific dipeptidaseAdd BLAST | 475 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 58 | PhosphoserineCombined sources | 1 | |
Modified residuei | 299 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q6Q0N1 |
PaxDbi | Q6Q0N1 |
PRIDEi | Q6Q0N1 |
PTM databases
iPTMneti | Q6Q0N1 |
PhosphoSitePlusi | Q6Q0N1 |
SwissPalmi | Q6Q0N1 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000015591, Expressed in adult mammalian kidney and 20 other tissues |
Genevisiblei | Q6Q0N1, RN |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 253502, 1 interactor |
IntActi | Q6Q0N1, 1 interactor |
MINTi | Q6Q0N1 |
STRINGi | 10116.ENSRNOP00000021400 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 166 – 167 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2276, Eukaryota |
GeneTreei | ENSGT00940000156500 |
HOGENOMi | CLU_029469_3_1_1 |
InParanoidi | Q6Q0N1 |
OMAi | HITIPGF |
OrthoDBi | 733473at2759 |
PhylomeDBi | Q6Q0N1 |
TreeFami | TF300633 |
Family and domain databases
InterProi | View protein in InterPro IPR001261, ArgE/DapE_CS IPR017153, CNDP/DUG1 IPR002933, Peptidase_M20 IPR011650, Peptidase_M20_dimer |
Pfami | View protein in Pfam PF07687, M20_dimer, 1 hit PF01546, Peptidase_M20, 1 hit |
PIRSFi | PIRSF037242, CNDP_dipeptidase, 1 hit |
PROSITEi | View protein in PROSITE PS00759, ARGE_DAPE_CPG2_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSALKAVFQY IDENQDRFVK KLAEWVAIQS VSAWPEKRGE IRRMTEAAAA
60 70 80 90 100
DVQRLGGSVE LVDIGKQKLP DGSEIPLPPI LLGKLGSDPQ KKTVCIYGHL
110 120 130 140 150
DVQPAALEDG WDSEPFTLVE REGKLYGRGS TDDKGPVAGW MNALEAYQKT
160 170 180 190 200
GQEIPVNLRF CLEGMEESGS EGLDELIFAQ KDKFFKDVDY VCISDNYWLG
210 220 230 240 250
KNKPCITYGL RGICYFFIEV ECSDKDLHSG VYGGSVHEAM TDLISLMGCL
260 270 280 290 300
IDKKGKILIP GINDAVAPVT DEEHELYDHI DFDMEEFAKD VGAGTLLHSC
310 320 330 340 350
KKDILMHRWR YPSLSLHGIE GAFSGSGAKT VIPRKVVGKF SIRLVPDMIP
360 370 380 390 400
EVVSEQVSSY LSKKFAELQS PNKFKVYMGH GGKPWVSDFN HPHYQAGRRA
410 420 430 440 450
LKTVFGVEPD LTREGGSIPV TLTFQEATGK NVMLLPVGSA DDGAHSQNEK
460 470
LNRLNYIEGT KMLAAYLYEV SQLKN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY569013 mRNA Translation: AAS75316.1 BC095904 mRNA Translation: AAH95904.1 |
RefSeqi | NP_001010920.1, NM_001010920.1 XP_006255061.1, XM_006254999.3 |
Genome annotation databases
Ensembli | ENSRNOT00000021400; ENSRNOP00000021400; ENSRNOG00000015591 |
GeneIDi | 291394 |
KEGGi | rno:291394 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY569013 mRNA Translation: AAS75316.1 BC095904 mRNA Translation: AAH95904.1 |
RefSeqi | NP_001010920.1, NM_001010920.1 XP_006255061.1, XM_006254999.3 |
3D structure databases
AlphaFoldDBi | Q6Q0N1 |
SMRi | Q6Q0N1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 253502, 1 interactor |
IntActi | Q6Q0N1, 1 interactor |
MINTi | Q6Q0N1 |
STRINGi | 10116.ENSRNOP00000021400 |
Protein family/group databases
MEROPSi | M20.005 |
PTM databases
iPTMneti | Q6Q0N1 |
PhosphoSitePlusi | Q6Q0N1 |
SwissPalmi | Q6Q0N1 |
Proteomic databases
jPOSTi | Q6Q0N1 |
PaxDbi | Q6Q0N1 |
PRIDEi | Q6Q0N1 |
Genome annotation databases
Ensembli | ENSRNOT00000021400; ENSRNOP00000021400; ENSRNOG00000015591 |
GeneIDi | 291394 |
KEGGi | rno:291394 |
Organism-specific databases
CTDi | 55748 |
RGDi | 1305827, Cndp2 |
Phylogenomic databases
eggNOGi | KOG2276, Eukaryota |
GeneTreei | ENSGT00940000156500 |
HOGENOMi | CLU_029469_3_1_1 |
InParanoidi | Q6Q0N1 |
OMAi | HITIPGF |
OrthoDBi | 733473at2759 |
PhylomeDBi | Q6Q0N1 |
TreeFami | TF300633 |
Enzyme and pathway databases
Reactomei | R-RNO-174403, Glutathione synthesis and recycling |
Miscellaneous databases
PROi | PR:Q6Q0N1 |
Gene expression databases
Bgeei | ENSRNOG00000015591, Expressed in adult mammalian kidney and 20 other tissues |
Genevisiblei | Q6Q0N1, RN |
Family and domain databases
InterProi | View protein in InterPro IPR001261, ArgE/DapE_CS IPR017153, CNDP/DUG1 IPR002933, Peptidase_M20 IPR011650, Peptidase_M20_dimer |
Pfami | View protein in Pfam PF07687, M20_dimer, 1 hit PF01546, Peptidase_M20, 1 hit |
PIRSFi | PIRSF037242, CNDP_dipeptidase, 1 hit |
PROSITEi | View protein in PROSITE PS00759, ARGE_DAPE_CPG2_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CNDP2_RAT | |
Accessioni | Q6Q0N1Primary (citable) accession number: Q6Q0N1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2007 |
Last sequence update: | July 5, 2004 | |
Last modified: | May 25, 2022 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families