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Protein

Ras-related protein Rab-35

Gene

Rab35

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.3 Publications

Activity regulationi

Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP) (By similarity). That Rab is activated by the guanine exchange factors DENND1A, DENND1B and DENND1C (PubMed:20159556, PubMed:26774822).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 23GTPBy similarity9
Nucleotide bindingi33 – 40GTPBy similarity8
Nucleotide bindingi63 – 67GTPBy similarity5
Nucleotide bindingi120 – 123GTPBy similarity4
Nucleotide bindingi150 – 152GTPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-8854214 TBC/RABGAPs
R-MMU-8873719 RAB geranylgeranylation
R-MMU-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-35
Gene namesi
Name:Rab35
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1924657 Rab35

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Coated pit, Cytoplasmic vesicle, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi22S → N: Loss of interaction with MICALL1. 2 Publications1
Mutagenesisi67Q → L: Loss of GTPase activity. Increased fast recycling. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001212461 – 201Ras-related protein Rab-35Add BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei72PhosphothreonineBy similarity1
Modified residuei75O-(2-cholinephosphoryl)serineBy similarity1
Lipidationi200S-geranylgeranyl cysteineBy similarity1
Lipidationi201S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

EPDiQ6PHN9
MaxQBiQ6PHN9
PaxDbiQ6PHN9
PRIDEiQ6PHN9

PTM databases

iPTMnetiQ6PHN9
PhosphoSitePlusiQ6PHN9
SwissPalmiQ6PHN9

Expressioni

Gene expression databases

BgeeiENSMUSG00000029518 Expressed in 241 organ(s), highest expression level in superior cervical ganglion
CleanExiMM_RAB35
ExpressionAtlasiQ6PHN9 baseline and differential
GenevisibleiQ6PHN9 MM

Interactioni

Subunit structurei

Interacts with DENND1A and DENND1B; in a nucleotide-dependent manner (PubMed:20159556, PubMed:26774822). Interacts with DENND1C; weak interaction which is nucleotide-independent (By similarity). Interacts (GTP-bound form) with ACAP2 and MICALL1; the interaction is direct and probably recruits ACAP2 and MICALL1 to membranes (PubMed:23572513). Interacts with EHD1; the interaction is indirect through MICALL1 and probably recruits EHD1 to membranes (PubMed:23572513).By similarity1 Publication

Protein-protein interaction databases

BioGridi218674, 2 interactors
DIPiDIP-38238N
IntActiQ6PHN9, 20 interactors
MINTiQ6PHN9
STRINGi10090.ENSMUSP00000031492

Structurei

3D structure databases

ProteinModelPortaliQ6PHN9
SMRiQ6PHN9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0079 Eukaryota
ENOG4111IWZ LUCA
GeneTreeiENSGT00900000140786
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiQ6PHN9
KOiK07876
OMAiLRVKLQI
OrthoDBiEOG091G0LA6
PhylomeDBiQ6PHN9
TreeFamiTF105954

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q6PHN9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MARDYDHLFK LLIIGDSGVG KSSLLLRFAD NTFSGSYITT IGVDFKIRTV
60 70 80 90 100
EINGEKVKLQ IWDTAGQERF RTITSTYYRG THGVIVVYDV TSAESFVNVK
110 120 130 140 150
RWLHEINQNC DDVCRILVGN KNDDPERKVV ETEDAYKFAG QMGIQLFETS
160 170 180 190 200
AKENVNVEEM FNCITELVLR AKKDNLAKQQ QQQQNDVVKL TKNSKRKKRC

C
Length:201
Mass (Da):23,025
Last modified:July 5, 2004 - v1
Checksum:i31EB15D6D42E076E
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
S4R1W7S4R1W7_MOUSE
Ras-related protein Rab-35
Rab35
55Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC056466 mRNA Translation: AAH56466.1
CCDSiCCDS19596.1
RefSeqiNP_937806.1, NM_198163.1
UniGeneiMm.27553

Genome annotation databases

EnsembliENSMUST00000031492; ENSMUSP00000031492; ENSMUSG00000029518
GeneIDi77407
KEGGimmu:77407
UCSCiuc008zei.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC056466 mRNA Translation: AAH56466.1
CCDSiCCDS19596.1
RefSeqiNP_937806.1, NM_198163.1
UniGeneiMm.27553

3D structure databases

ProteinModelPortaliQ6PHN9
SMRiQ6PHN9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218674, 2 interactors
DIPiDIP-38238N
IntActiQ6PHN9, 20 interactors
MINTiQ6PHN9
STRINGi10090.ENSMUSP00000031492

PTM databases

iPTMnetiQ6PHN9
PhosphoSitePlusiQ6PHN9
SwissPalmiQ6PHN9

Proteomic databases

EPDiQ6PHN9
MaxQBiQ6PHN9
PaxDbiQ6PHN9
PRIDEiQ6PHN9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031492; ENSMUSP00000031492; ENSMUSG00000029518
GeneIDi77407
KEGGimmu:77407
UCSCiuc008zei.1 mouse

Organism-specific databases

CTDi11021
MGIiMGI:1924657 Rab35

Phylogenomic databases

eggNOGiKOG0079 Eukaryota
ENOG4111IWZ LUCA
GeneTreeiENSGT00900000140786
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiQ6PHN9
KOiK07876
OMAiLRVKLQI
OrthoDBiEOG091G0LA6
PhylomeDBiQ6PHN9
TreeFamiTF105954

Enzyme and pathway databases

ReactomeiR-MMU-8854214 TBC/RABGAPs
R-MMU-8873719 RAB geranylgeranylation
R-MMU-8876198 RAB GEFs exchange GTP for GDP on RABs

Miscellaneous databases

ChiTaRSiRab35 mouse
PROiPR:Q6PHN9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029518 Expressed in 241 organ(s), highest expression level in superior cervical ganglion
CleanExiMM_RAB35
ExpressionAtlasiQ6PHN9 baseline and differential
GenevisibleiQ6PHN9 MM

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAB35_MOUSE
AccessioniPrimary (citable) accession number: Q6PHN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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