UniProtKB - Q6PGC1 (DHX29_MOUSE)
Protein
ATP-dependent RNA helicase DHX29
Gene
Dhx29
Organism
Mus musculus (Mouse)
Status
Functioni
ATP-binding RNA helicase involved in translation initiation. Part of the 43S pre-initiation complex that is required for efficient initiation on mRNAs of higher eukaryotes with structured 5'-UTRs by promoting efficient NTPase-dependent 48S complex formation. Specifically binds to the 40S ribosome near the mRNA entrance. Does not possess a processive helicase activity.UniRule annotation
Catalytic activityi
- EC:3.6.4.13UniRule annotation
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 594 – 601 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- nucleoside-triphosphatase activity Source: MGI
- ribosomal small subunit binding Source: UniProtKB
- RNA binding Source: GO_Central
- RNA helicase activity Source: UniProtKB-UniRule
- translation activator activity Source: MGI
- translation initiation factor activity Source: UniProtKB-KW
GO - Biological processi
- formation of translation preinitiation complex Source: MGI
- positive regulation of translational initiation Source: UniProtKB-UniRule
- ribosome assembly Source: MGI
Keywordsi
Molecular function | Helicase, Hydrolase, Initiation factor |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent RNA helicase DHX29UniRule annotation (EC:3.6.4.13UniRule annotation)Alternative name(s): DEAH box protein 29UniRule annotation |
Gene namesi | Name:Dhx29UniRule annotation |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2145374, Dhx29 |
Subcellular locationi
Other locations
- Cytoplasm UniRule annotation
Cytosol
- cytosolic small ribosomal subunit Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Other locations
- eukaryotic 43S preinitiation complex Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000245536 | 1 – 1365 | ATP-dependent RNA helicase DHX29Add BLAST | 1365 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 69 | PhosphoserineBy similarity | 1 | |
Modified residuei | 190 | PhosphoserineBy similarity | 1 | |
Modified residuei | 198 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q6PGC1 |
jPOSTi | Q6PGC1 |
MaxQBi | Q6PGC1 |
PaxDbi | Q6PGC1 |
PeptideAtlasi | Q6PGC1 |
PRIDEi | Q6PGC1 |
PTM databases
iPTMneti | Q6PGC1 |
PhosphoSitePlusi | Q6PGC1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000042426, Expressed in dorsal root ganglion and 261 other tissues |
Genevisiblei | Q6PGC1, MM |
Interactioni
Subunit structurei
Part of the 43S pre-initiation complex (PIC) that contains at least Met-tRNA, EIF1, EIF1A (EIF1AX or EIF1AY), EIF2S1, EIF2S2, EIF2S3, EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L, EIF3M, DHX29 and the 40S ribosomal subunit.
UniRule annotationProtein-protein interaction databases
BioGRIDi | 230051, 39 interactors |
IntActi | Q6PGC1, 1 interactor |
MINTi | Q6PGC1 |
STRINGi | 10090.ENSMUSP00000035244 |
Miscellaneous databases
RNActi | Q6PGC1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 581 – 754 | Helicase ATP-bindingUniRule annotationAdd BLAST | 174 | |
Domaini | 848 – 1025 | Helicase C-terminalUniRule annotationAdd BLAST | 178 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 281 – 308 | UniRule annotationAdd BLAST | 28 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 701 – 704 | DEAH boxUniRule annotation | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 10 – 53 | Ala-richAdd BLAST | 44 | |
Compositional biasi | 499 – 507 | Poly-Gln | 9 | |
Compositional biasi | 788 – 792 | Poly-Glu | 5 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0920, Eukaryota |
GeneTreei | ENSGT00940000157286 |
HOGENOMi | CLU_001832_1_4_1 |
InParanoidi | Q6PGC1 |
OMAi | WGASKCN |
OrthoDBi | 278674at2759 |
PhylomeDBi | Q6PGC1 |
TreeFami | TF324744 |
Family and domain databases
HAMAPi | MF_03068, DHX29, 1 hit |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR034730, DHX29 IPR002464, DNA/RNA_helicase_DEAH_CS IPR011709, DUF1605 IPR007502, Helicase-assoc_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF04408, HA2, 1 hit PF00271, Helicase_C, 1 hit PF07717, OB_NTP_bind, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00847, HA2, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00690, DEAH_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q6PGC1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGGKNKKHKA PGAAAMRAAV SASRARSAEA GAVGEAQSKK PVARPAPAVP
60 70 80 90 100
TGAREPRVKQ GPKIYSFNSA NDSGGSANLD KSILKVVINN KLEQRIIGVI
110 120 130 140 150
NEHKKQNSDR GAISGRLSAK KLQDLYMALQ AFSFKTKDIE DAMTNTLLHG
160 170 180 190 200
GDLHSALDWL CLNLSDDALP EGFSQEFEEQ QPKSRPKFQS VQIQATLSPP
210 220 230 240 250
QQTKTKRQEE DPKIKPKKEE TTVEVNMKEW ILRYAEQQDE EEKGEGSKGL
260 270 280 290 300
EEEEKFDPNQ RYLNLAARLL DAKEQAAAFK LEKNKQGQKE AQEKIRKFQR
310 320 330 340 350
EMETLEDHPI FNPAIKISHQ QNEKKKPAPA TEAESALNLN LFEKSAAATE
360 370 380 390 400
EEKGKKKEPH DVRNFDYTAR SWTGKSPKQF LIDWVRKNLP KSPNPSFEKV
410 420 430 440 450
AVGRYWKCRV RVVRSEDDVL VVCPTILTED GMQAQHLGAT LALYRLVKGQ
460 470 480 490 500
SVHQLLPPTY RDVWLEWSDE EKKREELNKM ETNKPRDLFI AKLLNKLKQQ
510 520 530 540 550
QQQQQQQRPE SEKGGSEDPE ESWENLVSDE DLAALSLEPT SAEDLAPVRS
560 570 580 590 600
LFRRLQSTPK YQRLLKERQQ LPVFKHRDSI VETLKRHRVV VVAGETGSGK
610 620 630 640 650
STQVPHFLLE DLLLDECGAR KCNIVCTQPR RISAVSLATR VCEELGCESG
660 670 680 690 700
PGGRNSLCGY QIRMESRASE STRLLYCTTG VLLRKLQEDG LLADVSHVIV
710 720 730 740 750
DEVHERSVQS DFLLVILKEI LQKRSDLHLI LMSATVDSDK FSTYFTHCPI
760 770 780 790 800
LRISGRSYPV EVFHLEDIVE ETGFVLEKDS EYCQKFLEEE EEITINVTSK
810 820 830 840 850
AGGVKKYQEY IPVQSGASPE LNPFYQKYSS RTQHAILYMN PHKINLDLIL
860 870 880 890 900
ELLVYLDKSP QFRNIEGAVL IFLPGLAHIQ QLYDLLSSDR RFYSERYQVI
910 920 930 940 950
ALHSVLSTQD QAAAFMFPPP GVRKIVLATN IAETGITIPD VVFVIDTGRT
960 970 980 990 1000
KENKYHESSQ MSSLVETFVS KASALQRQGR AGRVRDGFCF RLYTRERFEG
1010 1020 1030 1040 1050
FLDYSVPEIL RVPLEELCLH IMKCDLGSPE DFLSKALDPP QLQVISNAMN
1060 1070 1080 1090 1100
LLRKIGACEP NEPKLTPLGQ HLAALPVNVK IGKMLIFGAI FGCLEPVATL
1110 1120 1130 1140 1150
AAVMTEKSPF ITPIGRKDEA DLAKSSLAVA DSDHLTIYNA YLGWKKAQQE
1160 1170 1180 1190 1200
GGFRSEISYC QRNFLNRTSL LTLEDVKQEL MKLVKAAGFS SSPSWEGRKG
1210 1220 1230 1240 1250
PQTLSFQDIA LLKAVLAAGL YDSVGKIMCT KSVDVTEKLA CMVETAQGKA
1260 1270 1280 1290 1300
QVHPSSVNRD LQTYGWLLYQ EKVRYTRVYL RETTLITPFP VLLFGGDIEV
1310 1320 1330 1340 1350
QHRERLLSVD GWIYFQAPVK IAVIFKQLRV LIDSVLRKKL ENPKMSLEND
1360
KILQIITELI KTENN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ8BN72 | Q8BN72_MOUSE | ATP-dependent RNA helicase DHX29 | Dhx29 | 272 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC057112 mRNA Translation: AAH57112.1 BC082319 mRNA Translation: AAH82319.1 AK028342 mRNA Translation: BAC25894.1 AK042066 mRNA Translation: BAC31148.2 AK042497 mRNA Translation: BAC31274.2 AK049530 mRNA Translation: BAC33796.1 |
CCDSi | CCDS26779.1 |
RefSeqi | NP_766182.2, NM_172594.2 |
Genome annotation databases
Ensembli | ENSMUST00000038574; ENSMUSP00000035244; ENSMUSG00000042426 |
GeneIDi | 218629 |
KEGGi | mmu:218629 |
UCSCi | uc007rwx.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC057112 mRNA Translation: AAH57112.1 BC082319 mRNA Translation: AAH82319.1 AK028342 mRNA Translation: BAC25894.1 AK042066 mRNA Translation: BAC31148.2 AK042497 mRNA Translation: BAC31274.2 AK049530 mRNA Translation: BAC33796.1 |
CCDSi | CCDS26779.1 |
RefSeqi | NP_766182.2, NM_172594.2 |
3D structure databases
SMRi | Q6PGC1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230051, 39 interactors |
IntActi | Q6PGC1, 1 interactor |
MINTi | Q6PGC1 |
STRINGi | 10090.ENSMUSP00000035244 |
PTM databases
iPTMneti | Q6PGC1 |
PhosphoSitePlusi | Q6PGC1 |
Proteomic databases
EPDi | Q6PGC1 |
jPOSTi | Q6PGC1 |
MaxQBi | Q6PGC1 |
PaxDbi | Q6PGC1 |
PeptideAtlasi | Q6PGC1 |
PRIDEi | Q6PGC1 |
Protocols and materials databases
Antibodypediai | 11081, 90 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000038574; ENSMUSP00000035244; ENSMUSG00000042426 |
GeneIDi | 218629 |
KEGGi | mmu:218629 |
UCSCi | uc007rwx.1, mouse |
Organism-specific databases
CTDi | 54505 |
MGIi | MGI:2145374, Dhx29 |
Phylogenomic databases
eggNOGi | KOG0920, Eukaryota |
GeneTreei | ENSGT00940000157286 |
HOGENOMi | CLU_001832_1_4_1 |
InParanoidi | Q6PGC1 |
OMAi | WGASKCN |
OrthoDBi | 278674at2759 |
PhylomeDBi | Q6PGC1 |
TreeFami | TF324744 |
Miscellaneous databases
BioGRID-ORCSi | 218629, 11 hits in 20 CRISPR screens |
ChiTaRSi | Dhx29, mouse |
PROi | PR:Q6PGC1 |
RNActi | Q6PGC1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000042426, Expressed in dorsal root ganglion and 261 other tissues |
Genevisiblei | Q6PGC1, MM |
Family and domain databases
HAMAPi | MF_03068, DHX29, 1 hit |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR034730, DHX29 IPR002464, DNA/RNA_helicase_DEAH_CS IPR011709, DUF1605 IPR007502, Helicase-assoc_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF04408, HA2, 1 hit PF00271, Helicase_C, 1 hit PF07717, OB_NTP_bind, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00847, HA2, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00690, DEAH_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DHX29_MOUSE | |
Accessioni | Q6PGC1Primary (citable) accession number: Q6PGC1 Secondary accession number(s): Q8BQJ4 Q8C9H9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2006 |
Last sequence update: | July 5, 2004 | |
Last modified: | February 10, 2021 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families