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Protein

N-alpha-acetyltransferase 50

Gene

Naa50

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

N-alpha-acetyltransferase that acetylates the N-terminus of proteins that retain their initiating methionine. Has a broad substrate specificity: able to acetylate the initiator methionine of most peptides, except for those with a proline in second position. Also displays N-epsilon-acetyltransferase activity by mediating acetylation of the side chain of specific lysines on proteins. Autoacetylates in vivo. The relevance of N-epsilon-acetyltransferase activity is however unclear: able to acetylate H4 in vitro, but this result has not been confirmed in vivo. Component of a N-alpha-acetyltransferase complex containing NAA10 and NAA15, but NAA50 does not influence the acetyltransferase activity of NAA10: this multiprotein complex probably constitutes the major contributor for N-terminal acetylation at the ribosome exit tunnel, with NAA10 acetylating all amino termini that are devoid of methionine and NAA50 acetylating other peptides. Required for sister chromatid cohesion during mitosis by promoting binding of CDCA5/sororin to cohesin: may act by counteracting the function of NAA10.By similarity

Catalytic activityi

Acetyl-CoA + an N-terminal-L-methionyl-L-alanyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-alanyl-[protein] + CoA.By similarity
Acetyl-CoA + an N-terminal-L-methionyl-L-seryl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-seryl-[protein] + CoA.By similarity
Acetyl-CoA + an N-terminal-L-methionyl-L-valyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-valyl-[protein] + CoA.By similarity
Acetyl-CoA + an N-terminal-L-methionyl-L-threonyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-threonyl-[protein] + CoA.By similarity
Acetyl-CoA + an N-terminal-L-methionyl-L-lysyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-lysyl-[protein] + CoA.By similarity
Acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-leucyl-[protein] + CoA.By similarity
Acetyl-CoA + an N-terminal-L-methionyl-L-phenylalanyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-phenylalanyl-[protein] + CoA.By similarity
Acetyl-CoA + an N-terminal-L-methionyl-L-tyrosyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-tyrosyl-[protein] + CoA.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31SubstrateBy similarity1
Active sitei73By similarity1
Binding sitei75SubstrateBy similarity1
Active sitei112By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
N-alpha-acetyltransferase 50 (EC:2.3.1.258By similarity)
Alternative name(s):
N-acetyltransferase NAT13
N-epsilon-acetyltransferase 50By similarity (EC:2.3.1.-By similarity)
NatE catalytic subunit
Gene namesi
Name:Naa50Imported
Synonyms:Mak3, Nat13
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1919367 Naa50

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002849031 – 169N-alpha-acetyltransferase 50Add BLAST169

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphothreonineBy similarity1
Modified residuei34N6-acetyllysineCombined sources1
Modified residuei37N6-acetyllysineCombined sources1
Modified residuei110PhosphotyrosineBy similarity1
Modified residuei140N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ6PGB6
PaxDbiQ6PGB6
PeptideAtlasiQ6PGB6
PRIDEiQ6PGB6

PTM databases

iPTMnetiQ6PGB6
PhosphoSitePlusiQ6PGB6

Expressioni

Gene expression databases

BgeeiENSMUSG00000022698
CleanExiMM_NAT13
GenevisibleiQ6PGB6 MM

Interactioni

Subunit structurei

Component of a complex composed of NAA50, NAA15 and NAA10 (By similarity). Interacts with NAA35 (PubMed:16484612).By similarity1 Publication

Protein-protein interaction databases

BioGridi215162, 31 interactors
IntActiQ6PGB6, 32 interactors
MINTiQ6PGB6
STRINGi10090.ENSMUSP00000070140

Structurei

3D structure databases

ProteinModelPortaliQ6PGB6
SMRiQ6PGB6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 155N-acetyltransferasePROSITE-ProRule annotationAdd BLAST150

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni77 – 90Acetyl-CoA bindingBy similarityAdd BLAST14
Regioni117 – 126Coenzyme A bindingBy similarity10
Regioni138 – 141SubstrateBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3138 Eukaryota
COG0456 LUCA
GeneTreeiENSGT00390000009110
HOGENOMiHOG000238056
HOVERGENiHBG060820
InParanoidiQ6PGB6
KOiK20793
OMAiIVETKEH
OrthoDBiEOG091G0PAG
PhylomeDBiQ6PGB6
TreeFamiTF314841

Family and domain databases

InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR000182 GNAT_dom
PfamiView protein in Pfam
PF00583 Acetyltransf_1, 1 hit
SUPFAMiSSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS51186 GNAT, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6PGB6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKGSRIELGD VTPHNIKQLK RLNQVIFPVS YNDKFYKDVL EVGELAKLAY
60 70 80 90 100
FNDIAVGAVC CRVDHSQNQK RLYIMTLGCL APYRRLGIGT KMLNHVLNIC
110 120 130 140 150
EKDGTFDNIY LHVQISNESA IDFYRKFGFE IIETKKNYYK RIEPADAHVL
160
QKNLKVPSGQ NAETQKTDN
Length:169
Mass (Da):19,414
Last modified:July 5, 2004 - v1
Checksum:i153A8021B745347C
GO
Isoform 2 (identifier: Q6PGB6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     4-4: Missing.

Show »
Length:168
Mass (Da):19,327
Checksum:i3E0CE8B79B6DF970
GO
Isoform 3 (identifier: Q6PGB6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     49-88: Missing.

Note: No experimental confirmation available.
Show »
Length:129
Mass (Da):14,902
Checksum:iD36C0EE34082F3D8
GO
Isoform 4 (identifier: Q6PGB6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: MKG → MPGTVGHEH

Note: No experimental confirmation available.
Show »
Length:175
Mass (Da):20,044
Checksum:i49203461C605930C
GO
Isoform 5 (identifier: Q6PGB6-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     4-4: Missing.
     101-169: EKDGTFDNIY...QNAETQKTDN → GWMDGWMDGW...RALAKRRGHK

Note: No experimental confirmation available.
Show »
Length:162
Mass (Da):18,594
Checksum:iF8741E3E3447FB73
GO

Sequence cautioni

The sequence BAE40319 differs from that shown. Reason: Erroneous termination at position 170. Translated as stop.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0247481 – 3MKG → MPGTVGHEH in isoform 4. 1 Publication3
Alternative sequenceiVSP_0247494Missing in isoform 2 and isoform 5. 2 Publications1
Alternative sequenceiVSP_02475049 – 88Missing in isoform 3. 1 PublicationAdd BLAST40
Alternative sequenceiVSP_024751101 – 169EKDGT…QKTDN → GWMDGWMDGWMDGRASEIVE IPCLFLGYQFPKQSKSKLRS LVLSTFTLMDKLQRALAKRR GHK in isoform 5. 1 PublicationAdd BLAST69

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011160 mRNA Translation: BAB27439.1
AK135658 mRNA Translation: BAE22602.1
AK145345 mRNA Translation: BAE26378.1
AK156791 mRNA Translation: BAE33858.1
AK167141 mRNA Translation: BAE39286.1
AK168394 mRNA Translation: BAE40319.1 Sequence problems.
BC046283 mRNA Translation: AAH46283.1
BC055846 mRNA Translation: AAH55846.1
BC057117 mRNA Translation: AAH57117.1
CCDSiCCDS28183.1 [Q6PGB6-2]
CCDS84236.1 [Q6PGB6-1]
RefSeqiNP_001334168.1, NM_001347239.1 [Q6PGB6-1]
NP_082384.1, NM_028108.3 [Q6PGB6-2]
UniGeneiMm.278726

Genome annotation databases

EnsembliENSMUST00000063520; ENSMUSP00000070140; ENSMUSG00000022698 [Q6PGB6-2]
ENSMUST00000063542; ENSMUSP00000067361; ENSMUSG00000022698 [Q6PGB6-3]
ENSMUST00000159514; ENSMUSP00000125517; ENSMUSG00000022698 [Q6PGB6-4]
ENSMUST00000161326; ENSMUSP00000124291; ENSMUSG00000022698 [Q6PGB6-1]
GeneIDi72117
KEGGimmu:72117
UCSCiuc007zgy.1 mouse [Q6PGB6-2]
uc007zgz.1 mouse [Q6PGB6-4]
uc007zha.1 mouse [Q6PGB6-1]
uc012afx.1 mouse [Q6PGB6-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNAA50_MOUSE
AccessioniPrimary (citable) accession number: Q6PGB6
Secondary accession number(s): Q3TH79
, Q3TK59, Q7TML2, Q80VE3, Q9D0Q8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: July 5, 2004
Last modified: February 28, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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