UniProtKB - Q6PFH3 (DCA15_MOUSE)
Protein
DDB1- and CUL4-associated factor 15
Gene
Dcaf15
Organism
Mus musculus (Mouse)
Status
Functioni
Substrate-recognition component of the DCX(DCAF15) complex, a cullin-4-RING E3 ubiquitin-protein ligase complex that mediates ubiquitination and degradation of target proteins. The DCX(DCAF15) complex acts as a regulator of the natural killer (NK) cells effector functions, possibly by mediating ubiquitination and degradation of cohesin subunits SMC1A and SMC3. May play a role in the activation of antigen-presenting cells (APC) and their interaction with NK cells.By similarity
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 193 | ZincBy similarity | 1 | |
Metal bindingi | 196 | ZincBy similarity | 1 | |
Metal bindingi | 211 | ZincBy similarity | 1 | |
Metal bindingi | 214 | ZincBy similarity | 1 |
GO - Molecular functioni
- drug binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- small molecule binding Source: Ensembl
GO - Biological processi
- immune system process Source: UniProtKB-KW
- protein polyubiquitination Source: UniProtKB
- protein ubiquitination Source: GO_Central
- regulation of natural killer cell activation Source: UniProtKB
Keywordsi
Biological process | Immunity, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: DDB1- and CUL4-associated factor 15Curated |
Gene namesi | Name:Dcaf15Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2684420, Dcaf15 |
Subcellular locationi
Other locations
- Cul4-RING E3 ubiquitin ligase complex Source: UniProtKB
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000314486 | 1 – 600 | DDB1- and CUL4-associated factor 15Add BLAST | 600 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 50 | PhosphoserineBy similarity | 1 | |
Modified residuei | 314 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q6PFH3 |
PeptideAtlasi | Q6PFH3 |
PRIDEi | Q6PFH3 |
PTM databases
iPTMneti | Q6PFH3 |
PhosphoSitePlusi | Q6PFH3 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000037103, Expressed in ear vesicle and 283 other tissues |
ExpressionAtlasi | Q6PFH3, baseline and differential |
Genevisiblei | Q6PFH3, MM |
Interactioni
Subunit structurei
Component of the DCX(DCAF15) complex, also named CLR4(DCAF15) complex, composed of DCAF15, DDB1, cullin-4 (CUL4A or CUL4B), DDA1 and RBX1.
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000038568 |
Miscellaneous databases
RNActi | Q6PFH3, protein |
Family & Domainsi
Phylogenomic databases
eggNOGi | ENOG502QQCQ, Eukaryota |
GeneTreei | ENSGT00390000011987 |
HOGENOMi | CLU_031970_0_0_1 |
InParanoidi | Q6PFH3 |
OMAi | ACGISLC |
OrthoDBi | 471103at2759 |
PhylomeDBi | Q6PFH3 |
TreeFami | TF329680 |
Family and domain databases
InterProi | View protein in InterPro IPR038914, DCAF15 IPR032734, DCAF15_WD40 |
PANTHERi | PTHR28541, PTHR28541, 1 hit |
Pfami | View protein in Pfam PF14939, DCAF15_WD40, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q6PFH3-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAPSSKSERN SGAGSAGGGP GGTGGKRAVG RRREHVLKQL ERVKISGQLS
60 70 80 90 100
PRLFRKLPPR VCVSLKNIVD EDFLYAGHIF LGFSKCGRYV LSYTSSSGDD
110 120 130 140 150
DFSFYIYHLY WWEFNVHSKL KLVRQVRLFQ DEEIYSDLYL TVCEWPSDAS
160 170 180 190 200
KVIVFGFNTR SANGMLMNMM MMSDENHRDI YISTVAVPPR GRCAACQDAS
210 220 230 240 250
RAHPGDPSAQ CLRHGFMLHT KYQVVYPFPT FQPAFQLKKD QVVLLNTSYS
260 270 280 290 300
LVACAVSVHS AGDSSFCQIL YDHTALPPAP PSSPGPWSPE AAPAFPSLGV
310 320 330 340 350
EVVPAQPSGA PEPSPAIAKA KEFVADIFRR AKEAKGSPLE ETRLPSSLGP
360 370 380 390 400
SSSRCRPSLE PQAPSGEVVP RDSPPAAETT APEPGYINYT KLHYVLQSGE
410 420 430 440 450
GTEPEDEFED DKISLPFVVT DLRGRNLRPM RERTDMQGQY LTVEQLTLDF
460 470 480 490 500
EYVINEVIRH DATWGHQFCS FSDYDIVILE VCPETNQVLI NIGLLLLAFP
510 520 530 540 550
APTEEGQLRP KTYHTSLKVA WDLNTGIFET VSVGDLTEVK GQTSGSVWSS
560 570 580 590 600
YRKSCVDMVM KWLVPESSGR YVNRMTNEAL HKGCSLKVLA DSERYTWIVL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1B0GRT7 | A0A1B0GRT7_MOUSE | DDB1- and CUL4-associated factor 15 | Dcaf15 | 51 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 203 | H → N in BAC28110 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_030290 | 583 | G → GRKCPSWAGHLILPWIPGML DVRSVLGSEWHPAMAGEAG in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC057552 mRNA Translation: AAH57552.1 AK032982 mRNA Translation: BAC28110.1 AK153305 mRNA Translation: BAE31886.1 AK155000 mRNA Translation: BAE32985.1 |
CCDSi | CCDS40406.1 [Q6PFH3-2] CCDS90424.1 [Q6PFH3-1] |
RefSeqi | NP_766090.2, NM_172502.3 [Q6PFH3-2] XP_006530871.1, XM_006530808.1 |
Genome annotation databases
Ensembli | ENSMUST00000041367; ENSMUSP00000038568; ENSMUSG00000037103 [Q6PFH3-2] ENSMUST00000210279; ENSMUSP00000147690; ENSMUSG00000037103 [Q6PFH3-1] |
GeneIDi | 212123 |
KEGGi | mmu:212123 |
UCSCi | uc009mlw.1, mouse [Q6PFH3-2] uc009mlx.1, mouse [Q6PFH3-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC057552 mRNA Translation: AAH57552.1 AK032982 mRNA Translation: BAC28110.1 AK153305 mRNA Translation: BAE31886.1 AK155000 mRNA Translation: BAE32985.1 |
CCDSi | CCDS40406.1 [Q6PFH3-2] CCDS90424.1 [Q6PFH3-1] |
RefSeqi | NP_766090.2, NM_172502.3 [Q6PFH3-2] XP_006530871.1, XM_006530808.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000038568 |
PTM databases
iPTMneti | Q6PFH3 |
PhosphoSitePlusi | Q6PFH3 |
Proteomic databases
EPDi | Q6PFH3 |
PeptideAtlasi | Q6PFH3 |
PRIDEi | Q6PFH3 |
Protocols and materials databases
Antibodypediai | 77450, 2 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000041367; ENSMUSP00000038568; ENSMUSG00000037103 [Q6PFH3-2] ENSMUST00000210279; ENSMUSP00000147690; ENSMUSG00000037103 [Q6PFH3-1] |
GeneIDi | 212123 |
KEGGi | mmu:212123 |
UCSCi | uc009mlw.1, mouse [Q6PFH3-2] uc009mlx.1, mouse [Q6PFH3-1] |
Organism-specific databases
CTDi | 90379 |
MGIi | MGI:2684420, Dcaf15 |
Phylogenomic databases
eggNOGi | ENOG502QQCQ, Eukaryota |
GeneTreei | ENSGT00390000011987 |
HOGENOMi | CLU_031970_0_0_1 |
InParanoidi | Q6PFH3 |
OMAi | ACGISLC |
OrthoDBi | 471103at2759 |
PhylomeDBi | Q6PFH3 |
TreeFami | TF329680 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
BioGRID-ORCSi | 212123, 2 hits in 17 CRISPR screens |
PROi | PR:Q6PFH3 |
RNActi | Q6PFH3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037103, Expressed in ear vesicle and 283 other tissues |
ExpressionAtlasi | Q6PFH3, baseline and differential |
Genevisiblei | Q6PFH3, MM |
Family and domain databases
InterProi | View protein in InterPro IPR038914, DCAF15 IPR032734, DCAF15_WD40 |
PANTHERi | PTHR28541, PTHR28541, 1 hit |
Pfami | View protein in Pfam PF14939, DCAF15_WD40, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DCA15_MOUSE | |
Accessioni | Q6PFH3Primary (citable) accession number: Q6PFH3 Secondary accession number(s): Q3U305, Q8BMB2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 108 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways