UniProtKB - Q6PF39 (RUNX1_XENLA)
Protein
Runt-related transcription factor 1
Gene
runx1
Organism
Xenopus laevis (African clawed frog)
Status
Functioni
Involved in primitive hematopoiesis in the embryo.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 112 | Chloride 1PROSITE-ProRule annotationBy similarity | 1 | |
Binding sitei | 116 | Chloride 1; via amide nitrogenPROSITE-ProRule annotationBy similarity | 1 | |
Binding sitei | 139 | Chloride 2PROSITE-ProRule annotationBy similarity | 1 | |
Binding sitei | 170 | Chloride 2; via amide nitrogenPROSITE-ProRule annotationBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: InterPro
- calcium ion binding Source: UniProtKB
- DNA binding Source: UniProtKB
- DNA-binding transcription factor activity Source: UniProtKB
- protein heterodimerization activity Source: UniProtKB
GO - Biological processi
- multicellular organism development Source: UniProtKB-KW
- positive regulation of transcription, DNA-templated Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Chloride |
Names & Taxonomyi
Protein namesi | Recommended name: Runt-related transcription factor 1By similarityAlternative name(s): Acute myeloid leukemia 1 protein1 Publication Short name: XAMLImported Core-binding factor subunit alpha-21 Publication Short name: CBF-alpha-22 Publications |
Gene namesi | Name:runx1By similarity Synonyms:amlImported, cbfa21 Publication |
Organismi | Xenopus laevis (African clawed frog) |
Taxonomic identifieri | 8355 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Organism-specific databases
Xenbasei | XB-GENE-864949, runx1.L |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotationBy similarity
Nucleus
- nucleus Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000351207 | 1 – 462 | Runt-related transcription factor 1Add BLAST | 462 |
Expressioni
Tissue specificityi
Shows a complex and dynamic expression pattern. In stage 14-24 embryos, expressed in a subset of neuroblasts in the lateral stripe of the neural plate. In late neurula stages, expression begins in the olfactory placodes. Also expressed in structures that play a role in blood formation: at stage 14, expressed on the anterior ventral side of the embryo in the anterior endomesoderm. As the embryo elongates, expression shifts gradually to a V-shaped expression pattern in the presumptive ventral blood island.1 Publication
Developmental stagei
Expressed both maternally and zygotically. Shows a small amount of maternal expression. Zygotic expression begins at stage 8 and steadily increases after this point.1 Publication
Inductioni
By low levels of the dorsalizing factor LiCl. Inhibited by high doses of LiCl. In order to temporally control hematopoiesis, fgf-signaling inhibits expression in posterior regions of the embryo by antagonizing bmp-signaling.2 Publications
Interactioni
Subunit structurei
Heterodimer with cbfb. runx1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization (By similarity).
By similarityGO - Molecular functioni
- protein heterodimerization activity Source: UniProtKB
Protein-protein interaction databases
ELMi | Q6PF39 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 50 – 178 | RuntPROSITE-ProRule annotationAdd BLAST | 129 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 80 – 84 | Interaction with DNABy similarity | 5 | |
Regioni | 135 – 143 | Interaction with DNABy similarity | 9 | |
Regioni | 168 – 177 | Interaction with DNABy similarity | 10 |
Phylogenomic databases
OMAi | GRSGRXT |
OrthoDBi | 562214at2759 |
Family and domain databases
Gene3Di | 2.60.40.720, 1 hit 4.10.770.10, 1 hit |
InterProi | View protein in InterPro IPR000040, AML1_Runt IPR008967, p53-like_TF_DNA-bd IPR012346, p53/RUNT-type_TF_DNA-bd_sf IPR013524, Runt_dom IPR027384, Runx_central_dom_sf IPR013711, RunxI_C_dom IPR016554, TF_Runt-rel_RUNX |
PANTHERi | PTHR11950, PTHR11950, 1 hit |
Pfami | View protein in Pfam PF00853, Runt, 1 hit PF08504, RunxI, 1 hit |
PIRSFi | PIRSF009374, TF_Runt-rel_RUNX, 1 hit |
PRINTSi | PR00967, ONCOGENEAML1 |
SUPFAMi | SSF49417, SSF49417, 1 hit |
PROSITEi | View protein in PROSITE PS51062, RUNT, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 11 Publication (identifier: Q6PF39-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRIPVDTSTS RRFTPPSTTL SPGKMSEPIP LNIADSSAAL VGKLRSTDRN
60 70 80 90 100
MVEVLSDHPG ELVRTDSPNF LCSVLPTHWR CNKTLPIAFK VVALGEVPDG
110 120 130 140 150
TLVTVMAGND ENYSAELRNA TAAMKSQVAR FNDLRFVGRS GRGKSFTLTI
160 170 180 190 200
TVFTNPPQVA TYHRAIKITV DGPREPRRHR QKLDEQTKPG NLSFSERLSE
210 220 230 240 250
LEHFRRTAMR VSPHHPNPMP NPRATLNHSA AFNPQPQGQI QVADTRQVQA
260 270 280 290 300
SPPWSYDQSY QYLGSIATQS VHPATPISPG RASSMTSLSA ELSSRLSGAS
310 320 330 340 350
DLTAFSDPRV GIDRQFSTLP SISDPRMHYP GAFTYTPTPV TSGIGIGMSA
360 370 380 390 400
MTSATRYHTY LPPPYPGSSQ AQSNPFQTSS PSYHLYYGTS AGSYHQFSMM
410 420 430 440 450
SGGERSPPRI LPPCTNASTG STLLNPNLPN QSDVVEAEGS HSNSPTNMGS
460
TPRLEEAVWR PY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 74 | V → L in AAC41269 (PubMed:9502719).Curated | 1 | |
Sequence conflicti | 195 | S → P in AAC41269 (PubMed:9502719).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_052947 | 1 – 5 | MRIPV → MASHSAFQSFPLYPPCFFR in isoform 2. 1 Publication | 5 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF035446 mRNA Translation: AAC41269.1 BC057739 mRNA Translation: AAH57739.1 |
RefSeqi | NP_001079966.1, NM_001086497.1 [Q6PF39-2] XP_018100278.1, XM_018244789.1 [Q6PF39-2] |
Genome annotation databases
GeneIDi | 379657 |
KEGGi | xla:379657 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF035446 mRNA Translation: AAC41269.1 BC057739 mRNA Translation: AAH57739.1 |
RefSeqi | NP_001079966.1, NM_001086497.1 [Q6PF39-2] XP_018100278.1, XM_018244789.1 [Q6PF39-2] |
3D structure databases
SMRi | Q6PF39 |
ModBasei | Search... |
Protein-protein interaction databases
ELMi | Q6PF39 |
Genome annotation databases
GeneIDi | 379657 |
KEGGi | xla:379657 |
Organism-specific databases
CTDi | 379657 |
Xenbasei | XB-GENE-864949, runx1.L |
Phylogenomic databases
OMAi | GRSGRXT |
OrthoDBi | 562214at2759 |
Family and domain databases
Gene3Di | 2.60.40.720, 1 hit 4.10.770.10, 1 hit |
InterProi | View protein in InterPro IPR000040, AML1_Runt IPR008967, p53-like_TF_DNA-bd IPR012346, p53/RUNT-type_TF_DNA-bd_sf IPR013524, Runt_dom IPR027384, Runx_central_dom_sf IPR013711, RunxI_C_dom IPR016554, TF_Runt-rel_RUNX |
PANTHERi | PTHR11950, PTHR11950, 1 hit |
Pfami | View protein in Pfam PF00853, Runt, 1 hit PF08504, RunxI, 1 hit |
PIRSFi | PIRSF009374, TF_Runt-rel_RUNX, 1 hit |
PRINTSi | PR00967, ONCOGENEAML1 |
SUPFAMi | SSF49417, SSF49417, 1 hit |
PROSITEi | View protein in PROSITE PS51062, RUNT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RUNX1_XENLA | |
Accessioni | Q6PF39Primary (citable) accession number: Q6PF39 Secondary accession number(s): O73725 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 23, 2008 |
Last sequence update: | September 23, 2008 | |
Last modified: | December 2, 2020 | |
This is version 80 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |