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Protein

Presequence protease, mitochondrial

Gene

pitrm1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Metalloendopeptidase of the mitochondrial matrix that functions in peptide cleavage and degradation rather than in protein processing. Has an ATP-independent activity. Specifically cleaves peptides in the range of 5 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. Degrades the transit peptides of mitochondrial proteins after their cleavage. Also degrades other unstructured peptides.By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Mainly exists in a closed and catalytically competent conformation but a closed-to-open switch allows substrate entry into the catalytic chamber. Substrate binding induces closure and dimerization. A disulfide bond may lock the enzyme in a closed conformation preventing substrate entry into the catalytic chamber, participating in redox regulation of the enzyme. Inhibited by metal-chelating agents. Inhibited by nickel and zinc excess, and slightly activated by manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi98Zinc; via tele nitrogen; catalyticBy similarity1
Active sitei101Proton acceptorBy similarity1
Metal bindingi102Zinc; via tele nitrogen; catalyticBy similarity1
Metal bindingi199Zinc; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Presequence protease, mitochondrialCurated (EC:3.4.24.-By similarity)
Alternative name(s):
Pitrilysin metalloproteinase 1By similarity
Gene namesi
Name:pitrm1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-952663 pitrm1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 22MitochondrionSequence analysisAdd BLAST22
ChainiPRO_000024993523 – 1027Presequence protease, mitochondrialAdd BLAST1005

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi113 ↔ 550By similarity

Post-translational modificationi

A disulfide bond locks the enzyme in the closed conformation preventing substrate entry into the catalytic chamber.By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ6PF24

Interactioni

Subunit structurei

Monomer and homodimer; homodimerization is induced by binding of the substrate.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6PF24
SMRiQ6PF24
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M16 family. PreP subfamily.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOVERGENiHBG082167
KOiK06972

Family and domain databases

InterProiView protein in InterPro
IPR011249 Metalloenz_LuxS/M16
IPR011765 Pept_M16_N
IPR007863 Peptidase_M16_C
IPR013578 Peptidase_M16C_assoc
PfamiView protein in Pfam
PF08367 M16C_assoc, 1 hit
PF00675 Peptidase_M16, 1 hit
PF05193 Peptidase_M16_C, 2 hits
SMARTiView protein in SMART
SM01264 M16C_associated, 1 hit
SUPFAMiSSF63411 SSF63411, 4 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6PF24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRQCWAGLR LCRALYQTSY RWHGKSACER ALRYSPGESI HGFTVNEVTP
60 70 80 90 100
VPELFLTAVK LSHDNTGAKY LHVAREDSNN LFSVQFRTTP LDSTGVPHIL
110 120 130 140 150
EHTVLCGSQK YPCRDPFFKM LNRSLSTFMN AFTASDYTMY PFSTQNAKDF
160 170 180 190 200
QNLLSVYLDA VFFPCLRELD FWQEGWRLEH ENPEDPNSPL IFKGIVFNEM
210 220 230 240 250
KGAFTDNEKV FSQHLQNKLL PDHTYSVVSG GEPLNIPDLT WEQLKEFHAT
260 270 280 290 300
HYHPSNARFF TYGNLPLEMH LKQIHEDALS KFGRIDPKTS VPPQERWQSP
310 320 330 340 350
REYSISCGVD SFASDPEKQT MVSVNFLLSE ITDSFEAFTL SLLSSLMVDG
360 370 380 390 400
PNSPFYKALI EANLGTDFSP DTGFNNYTRE TYFSIGLQGI NKEDSEKVKH
410 420 430 440 450
IINRTINEIA EQGIEPERIE ALLHKLEIQM KHQSTSFGLT LASYIASCWN
460 470 480 490 500
HEGDPVDLLK IGDKISRFRQ CLKENPKFLQ DKVKQYFQVN QHRMMLSMSP
510 520 530 540 550
DEQHYDKEEQ LEEEKLTQKV KALSEEERKQ IYEKGLELIS LQSKPQDFSC
560 570 580 590 600
LPALKVSDIE PQIPLTDLEI AYAGDVPVQY CTQPTNGMVY FRAVSSLNTL
610 620 630 640 650
PEELKPYVPL FCSVITKLGC GVYNYREQAQ QMELTTGGMS VCPHIISDDS
660 670 680 690 700
SLDTYEQGIL FSSLCLDRNM PDMMHLWSEI FNSPHFDDEE RLRVLVRMSA
710 720 730 740 750
QEMSNGIPDS GHVYASIRAS RTLTPTGELQ ELFSGMDQVK MIKRIAEMPD
760 770 780 790 800
MGSILRKLSR IRKYVLLSDN MRCSINAAPQ QMETASKEME HFLTGITRSK
810 820 830 840 850
KERKAIRPHV VEKSSNPSPS GSEISRTATR KLVGDPTFKP CQMKTHFCLS
860 870 880 890 900
FPVNYIGECV RTVPYTHPDY ASLRILARIM TAKFLHGEIR EKGGAYGGGA
910 920 930 940 950
KLSFDGIFGF YSYRDPNSLS TLSTFQKATD WAKSGQFSQQ DVDEAKLSVF
960 970 980 990 1000
SAVDSPIAPS DKGMNHFLHG ISDEMKQRHR EELFAVTHSD LTNASNKYLT
1010 1020
AGQCTRGTAI LGPENKNIAK DPSWIIR
Length:1,027
Mass (Da):116,680
Last modified:July 5, 2004 - v1
Checksum:i64174B1B6ED59822
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC057754 mRNA Translation: AAH57754.1
RefSeqiNP_001079981.1, NM_001086512.1
UniGeneiXl.18714

Genome annotation databases

GeneIDi379672
KEGGixla:379672

Similar proteinsi

Entry informationi

Entry nameiPREP_XENLA
AccessioniPrimary (citable) accession number: Q6PF24
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: July 5, 2004
Last modified: June 20, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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