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Protein

Microtubule-associated protein RP/EB family member 3

Gene

Mapre3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (By similarity). May play a role in cell migration (By similarity).By similarity

GO - Molecular functioni

  • identical protein binding Source: MGI
  • microtubule binding Source: BHF-UCL
  • microtubule plus-end binding Source: GO_Central
  • protein C-terminus binding Source: MGI
  • protein kinase binding Source: MGI

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated protein RP/EB family member 3
Alternative name(s):
EB1 protein family member 3
Short name:
EBF3
End-binding protein 3
Short name:
EB3
RP3
Gene namesi
Name:Mapre3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2140967 Mapre3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002134291 – 281Microtubule-associated protein RP/EB family member 3Add BLAST281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei162PhosphoserineCombined sources1
Modified residuei176PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6PER3
PaxDbiQ6PER3
PRIDEiQ6PER3

PTM databases

iPTMnetiQ6PER3
PhosphoSitePlusiQ6PER3

Expressioni

Gene expression databases

BgeeiENSMUSG00000029166 Expressed in 214 organ(s), highest expression level in brain
ExpressionAtlasiQ6PER3 baseline and differential
GenevisibleiQ6PER3 MM

Interactioni

Subunit structurei

Homodimer (By similarity). Heterodimer with MAPRE1 (By similarity). Interacts with DCTN1 and SRCIN1 (By similarity). Binds to the C-terminal domain of APC (By similarity). Binds monomeric and polymerized tubulin. Interacts (via C-terminus) with CLIP1 (By similarity). Interacts with SLAIN2 (By similarity). Interacts with SLAIN1 (PubMed:21646404). Interacts with APC2 (PubMed:17310996). Interacts with AKAP9 (By similarity). Interacts with PDE4DIP isoform 2/MMG8/SMYLE; this interaction is required for its recruitment to the Golgi apparatus (By similarity).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi221519, 2 interactors
IntActiQ6PER3, 2 interactors
MINTiQ6PER3
STRINGi10090.ENSMUSP00000031058

Structurei

3D structure databases

ProteinModelPortaliQ6PER3
SMRiQ6PER3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 116Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST103
Domaini194 – 264EB1 C-terminalPROSITE-ProRule annotationAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni217 – 281DCTN1-bindingBy similarityAdd BLAST65
Regioni217 – 260APC-bindingBy similarityAdd BLAST44

Domaini

Composed of two functionally independent domains. The N-terminal domain forms a hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.By similarity

Sequence similaritiesi

Belongs to the MAPRE family.Curated

Phylogenomic databases

eggNOGiKOG3000 Eukaryota
COG5217 LUCA
GeneTreeiENSGT00490000043329
HOGENOMiHOG000198048
HOVERGENiHBG052410
InParanoidiQ6PER3
KOiK10436
OMAiTPQRVQH
OrthoDBiEOG091G0FT4
PhylomeDBiQ6PER3
TreeFamiTF313620

Family and domain databases

Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR004953 EB1_C
IPR036133 EB1_C_sf
IPR027738 EB3
IPR027328 MAPRE
PANTHERiPTHR10623 PTHR10623, 1 hit
PTHR10623:SF10 PTHR10623:SF10, 1 hit
PfamiView protein in Pfam
PF00307 CH, 1 hit
PF03271 EB1, 1 hit
SUPFAMiSSF140612 SSF140612, 1 hit
SSF47576 SSF47576, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit
PS51230 EB1_C, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q6PER3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAVNVYSTSV TSENLSRHDM LAWVNDSLHL NYTKIEQLCS GAAYCQFMDM
60 70 80 90 100
LFPGCVHLRK VKFQAKLEHE YIHNFKVLQA AFKKMGVDKI IPVEKLVKGK
110 120 130 140 150
FQDNFEFIQW FKKFFDANYD GKDYNPLLAR QGQDVAPPPN PGDQIFNKSK
160 170 180 190 200
KLIGTAVPQR TSPTGPKNMQ TSGRLSNVAP PCILRKNPPS ARNGGHEADA
210 220 230 240 250
QILELNQQLL DLKLTVDGLE KERDFYFSKL RDIELICQEH ESENSPVISG
260 270 280
IIGILYATEE GFAPPEDDEI EEHQQEDQDE Y
Length:281
Mass (Da):31,966
Last modified:July 5, 2004 - v1
Checksum:i6713427C480838DC
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z6G3D3Z6G3_MOUSE
Microtubule-associated protein RP/E...
Mapre3
266Annotation score:
A0A0J9YVB9A0A0J9YVB9_MOUSE
Microtubule-associated protein RP/E...
Mapre3
140Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC057918 mRNA Translation: AAH57918.1
U51204 mRNA Translation: AAA96321.1
CCDSiCCDS19163.1
RefSeqiNP_579928.1, NM_133350.2
XP_006503686.1, XM_006503623.3
UniGeneiMm.332295
Mm.471154

Genome annotation databases

EnsembliENSMUST00000031058; ENSMUSP00000031058; ENSMUSG00000029166
GeneIDi100732
KEGGimmu:100732
UCSCiuc008wvz.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC057918 mRNA Translation: AAH57918.1
U51204 mRNA Translation: AAA96321.1
CCDSiCCDS19163.1
RefSeqiNP_579928.1, NM_133350.2
XP_006503686.1, XM_006503623.3
UniGeneiMm.332295
Mm.471154

3D structure databases

ProteinModelPortaliQ6PER3
SMRiQ6PER3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221519, 2 interactors
IntActiQ6PER3, 2 interactors
MINTiQ6PER3
STRINGi10090.ENSMUSP00000031058

PTM databases

iPTMnetiQ6PER3
PhosphoSitePlusiQ6PER3

Proteomic databases

MaxQBiQ6PER3
PaxDbiQ6PER3
PRIDEiQ6PER3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031058; ENSMUSP00000031058; ENSMUSG00000029166
GeneIDi100732
KEGGimmu:100732
UCSCiuc008wvz.1 mouse

Organism-specific databases

CTDi22924
MGIiMGI:2140967 Mapre3

Phylogenomic databases

eggNOGiKOG3000 Eukaryota
COG5217 LUCA
GeneTreeiENSGT00490000043329
HOGENOMiHOG000198048
HOVERGENiHBG052410
InParanoidiQ6PER3
KOiK10436
OMAiTPQRVQH
OrthoDBiEOG091G0FT4
PhylomeDBiQ6PER3
TreeFamiTF313620

Miscellaneous databases

ChiTaRSiMapre3 mouse
PROiPR:Q6PER3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029166 Expressed in 214 organ(s), highest expression level in brain
ExpressionAtlasiQ6PER3 baseline and differential
GenevisibleiQ6PER3 MM

Family and domain databases

Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR004953 EB1_C
IPR036133 EB1_C_sf
IPR027738 EB3
IPR027328 MAPRE
PANTHERiPTHR10623 PTHR10623, 1 hit
PTHR10623:SF10 PTHR10623:SF10, 1 hit
PfamiView protein in Pfam
PF00307 CH, 1 hit
PF03271 EB1, 1 hit
SUPFAMiSSF140612 SSF140612, 1 hit
SSF47576 SSF47576, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit
PS51230 EB1_C, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMARE3_MOUSE
AccessioniPrimary (citable) accession number: Q6PER3
Secondary accession number(s): Q61167
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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