UniProtKB - Q6PER3 (MARE3_MOUSE)
Microtubule-associated protein RP/EB family member 3
Mapre3
Functioni
Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (By similarity).
May play a role in cell migration (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
- microtubule binding Source: BHF-UCL
- microtubule plus-end binding Source: MGI
- protein C-terminus binding Source: MGI
- protein kinase binding Source: MGI
GO - Biological processi
- cell division Source: UniProtKB-KW
- positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: BHF-UCL
- positive regulation of microtubule plus-end binding Source: MGI
- positive regulation of protein kinase activity Source: MGI
- positive regulation of transcription, DNA-templated Source: BHF-UCL
- protein localization to microtubule plus-end Source: GO_Central
- regulation of microtubule polymerization Source: MGI
- regulation of microtubule polymerization or depolymerization Source: GO_Central
- spindle assembly Source: GO_Central
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis |
Names & Taxonomyi
Protein namesi | Recommended name: Microtubule-associated protein RP/EB family member 3Alternative name(s): EB1 protein family member 3 Short name: EBF3 End-binding protein 3 Short name: EB3 RP3 |
Gene namesi | Name:Mapre3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2140967, Mapre3 |
VEuPathDBi | HostDB:ENSMUSG00000029166 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Note: Associated with the microtubule network. Detected at the plus end of microtubules.1 Publication
Cytoskeleton
- cytoplasmic microtubule Source: GO_Central
- microtubule cytoskeleton Source: BHF-UCL
- microtubule organizing center Source: GO_Central
- microtubule plus-end Source: MGI
- mitotic spindle astral microtubule end Source: MGI
- spindle midzone Source: GO_Central
Other locations
- cytoplasm Source: BHF-UCL
- midbody Source: BHF-UCL
- perinuclear region of cytoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, MicrotubulePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213429 | 1 – 281 | Microtubule-associated protein RP/EB family member 3Add BLAST | 281 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 162 | PhosphoserineCombined sources | 1 | |
Modified residuei | 176 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q6PER3 |
PaxDbi | Q6PER3 |
PRIDEi | Q6PER3 |
ProteomicsDBi | 252731 |
PTM databases
iPTMneti | Q6PER3 |
PhosphoSitePlusi | Q6PER3 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000029166, Expressed in brain and 241 other tissues |
Genevisiblei | Q6PER3, MM |
Interactioni
Subunit structurei
Homodimer (By similarity). Heterodimer with MAPRE1 (By similarity).
Interacts with DCTN1 and SRCIN1 (By similarity). Binds to the C-terminal domain of APC (By similarity). Binds monomeric and polymerized tubulin.
Interacts (via C-terminus) with CLIP1 (By similarity).
Interacts with SLAIN2 (By similarity).
Interacts with SLAIN1 (PubMed:21646404).
Interacts with APC2 (PubMed:17310996).
Interacts with AKAP9 (By similarity).
Interacts with PDE4DIP isoform 2/MMG8/SMYLE; this interaction is required for its recruitment to the Golgi apparatus (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- identical protein binding Source: MGI
- microtubule binding Source: BHF-UCL
- microtubule plus-end binding Source: MGI
- protein C-terminus binding Source: MGI
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 221519, 9 interactors |
IntActi | Q6PER3, 1 interactor |
MINTi | Q6PER3 |
STRINGi | 10090.ENSMUSP00000031058 |
Miscellaneous databases
RNActi | Q6PER3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 14 – 116 | Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST | 103 | |
Domaini | 194 – 264 | EB1 C-terminalPROSITE-ProRule annotationAdd BLAST | 71 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 157 – 181 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 217 – 281 | DCTN1-bindingBy similarityAdd BLAST | 65 | |
Regioni | 217 – 260 | APC-bindingBy similarityAdd BLAST | 44 | |
Regioni | 260 – 281 | DisorderedSequence analysisAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 157 – 177 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 262 – 281 | Acidic residuesSequence analysisAdd BLAST | 20 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3000, Eukaryota |
GeneTreei | ENSGT00490000043329 |
InParanoidi | Q6PER3 |
OMAi | TFAKHQI |
OrthoDBi | 1237523at2759 |
PhylomeDBi | Q6PER3 |
TreeFami | TF313620 |
Family and domain databases
Gene3Di | 1.10.418.10, 1 hit |
InterProi | View protein in InterPro IPR001715, CH-domain IPR036872, CH_dom_sf IPR004953, EB1_C IPR036133, EB1_C_sf IPR027738, EB3 IPR027328, MAPRE |
PANTHERi | PTHR10623, PTHR10623, 1 hit PTHR10623:SF10, PTHR10623:SF10, 1 hit |
Pfami | View protein in Pfam PF00307, CH, 1 hit PF03271, EB1, 1 hit |
SUPFAMi | SSF140612, SSF140612, 1 hit SSF47576, SSF47576, 1 hit |
PROSITEi | View protein in PROSITE PS50021, CH, 1 hit PS51230, EB1_C, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAVNVYSTSV TSENLSRHDM LAWVNDSLHL NYTKIEQLCS GAAYCQFMDM
60 70 80 90 100
LFPGCVHLRK VKFQAKLEHE YIHNFKVLQA AFKKMGVDKI IPVEKLVKGK
110 120 130 140 150
FQDNFEFIQW FKKFFDANYD GKDYNPLLAR QGQDVAPPPN PGDQIFNKSK
160 170 180 190 200
KLIGTAVPQR TSPTGPKNMQ TSGRLSNVAP PCILRKNPPS ARNGGHEADA
210 220 230 240 250
QILELNQQLL DLKLTVDGLE KERDFYFSKL RDIELICQEH ESENSPVISG
260 270 280
IIGILYATEE GFAPPEDDEI EEHQQEDQDE Y
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3Z6G3 | D3Z6G3_MOUSE | Microtubule-associated protein RP/E... | Mapre3 | 266 | Annotation score: | ||
A0A0J9YVB9 | A0A0J9YVB9_MOUSE | Microtubule-associated protein RP/E... | Mapre3 | 140 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC057918 mRNA Translation: AAH57918.1 U51204 mRNA Translation: AAA96321.1 |
CCDSi | CCDS19163.1 |
RefSeqi | NP_579928.1, NM_133350.2 XP_006503686.1, XM_006503623.3 |
Genome annotation databases
Ensembli | ENSMUST00000031058; ENSMUSP00000031058; ENSMUSG00000029166 |
GeneIDi | 100732 |
KEGGi | mmu:100732 |
UCSCi | uc008wvz.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC057918 mRNA Translation: AAH57918.1 U51204 mRNA Translation: AAA96321.1 |
CCDSi | CCDS19163.1 |
RefSeqi | NP_579928.1, NM_133350.2 XP_006503686.1, XM_006503623.3 |
3D structure databases
BMRBi | Q6PER3 |
SMRi | Q6PER3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 221519, 9 interactors |
IntActi | Q6PER3, 1 interactor |
MINTi | Q6PER3 |
STRINGi | 10090.ENSMUSP00000031058 |
PTM databases
iPTMneti | Q6PER3 |
PhosphoSitePlusi | Q6PER3 |
Proteomic databases
MaxQBi | Q6PER3 |
PaxDbi | Q6PER3 |
PRIDEi | Q6PER3 |
ProteomicsDBi | 252731 |
Protocols and materials databases
Antibodypediai | 1949, 262 antibodies from 38 providers |
DNASUi | 100732 |
Genome annotation databases
Ensembli | ENSMUST00000031058; ENSMUSP00000031058; ENSMUSG00000029166 |
GeneIDi | 100732 |
KEGGi | mmu:100732 |
UCSCi | uc008wvz.1, mouse |
Organism-specific databases
CTDi | 22924 |
MGIi | MGI:2140967, Mapre3 |
VEuPathDBi | HostDB:ENSMUSG00000029166 |
Phylogenomic databases
eggNOGi | KOG3000, Eukaryota |
GeneTreei | ENSGT00490000043329 |
InParanoidi | Q6PER3 |
OMAi | TFAKHQI |
OrthoDBi | 1237523at2759 |
PhylomeDBi | Q6PER3 |
TreeFami | TF313620 |
Miscellaneous databases
BioGRID-ORCSi | 100732, 2 hits in 65 CRISPR screens |
ChiTaRSi | Mapre3, mouse |
PROi | PR:Q6PER3 |
RNActi | Q6PER3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029166, Expressed in brain and 241 other tissues |
Genevisiblei | Q6PER3, MM |
Family and domain databases
Gene3Di | 1.10.418.10, 1 hit |
InterProi | View protein in InterPro IPR001715, CH-domain IPR036872, CH_dom_sf IPR004953, EB1_C IPR036133, EB1_C_sf IPR027738, EB3 IPR027328, MAPRE |
PANTHERi | PTHR10623, PTHR10623, 1 hit PTHR10623:SF10, PTHR10623:SF10, 1 hit |
Pfami | View protein in Pfam PF00307, CH, 1 hit PF03271, EB1, 1 hit |
SUPFAMi | SSF140612, SSF140612, 1 hit SSF47576, SSF47576, 1 hit |
PROSITEi | View protein in PROSITE PS50021, CH, 1 hit PS51230, EB1_C, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MARE3_MOUSE | |
Accessioni | Q6PER3Primary (citable) accession number: Q6PER3 Secondary accession number(s): Q61167 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2005 |
Last sequence update: | July 5, 2004 | |
Last modified: | February 23, 2022 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families