Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

S-phase kinase-associated protein 1

Gene

Skp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) direct ubiquitination of BCL2.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-1169091 Activation of NF-kappaB in B cells
R-RNO-1170546 Prolactin receptor signaling
R-RNO-174113 SCF-beta-TrCP mediated degradation of Emi1
R-RNO-187577 SCF(Skp2)-mediated degradation of p27/p21
R-RNO-195253 Degradation of beta-catenin by the destruction complex
R-RNO-202424 Downstream TCR signaling
R-RNO-2565942 Regulation of PLK1 Activity at G2/M Transition
R-RNO-2871837 FCERI mediated NF-kB activation
R-RNO-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-RNO-5607764 CLEC7A (Dectin-1) signaling
R-RNO-5610780 Degradation of GLI1 by the proteasome
R-RNO-5610785 GLI3 is processed to GLI3R by the proteasome
R-RNO-5676590 NIK-->noncanonical NF-kB signaling
R-RNO-5684264 MAP3K8 (TPL2)-dependent MAPK1/3 activation
R-RNO-68949 Orc1 removal from chromatin
R-RNO-69231 Cyclin D associated events in G1
R-RNO-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-RNO-8939902 Regulation of RUNX2 expression and activity
R-RNO-8951664 Neddylation
R-RNO-9020702 Interleukin-1 signaling
R-RNO-917937 Iron uptake and transport
R-RNO-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
S-phase kinase-associated protein 1
Alternative name(s):
Cyclin-A/CDK2-associated protein p19
S-phase kinase-associated protein 1A
p19A
p19skp1
Gene namesi
Name:Skp1
Synonyms:Skp1a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1359648 Skp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872531 – 163S-phase kinase-associated protein 1Add BLAST163

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei131PhosphothreonineCombined sources1
Cross-linki142Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6PEC4
PRIDEiQ6PEC4

PTM databases

iPTMnetiQ6PEC4
PhosphoSitePlusiQ6PEC4
SwissPalmiQ6PEC4

Expressioni

Gene expression databases

BgeeiENSRNOG00000005828 Expressed in 10 organ(s), highest expression level in adult mammalian kidney
ExpressionAtlasiQ6PEC4 baseline and differential
GenevisibleiQ6PEC4 RN

Interactioni

Subunit structurei

Component of multiple SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complexes formed of CUL1, SKP1, RBX1 and a variable F-box domain-containing protein as substrate-specific subunit. Component of the SCF(FBXW11) complex containing FBXW11. Component of the SCF(SKP2) complex containing SKP2, in which it interacts directly with SKP1, SKP2 and RBX1. Component of the SCF(FBXW2) complex containing FBXw2. Component of the SCF(FBXO32) complex containing FBXO32. Component of the probable SCF(FBXO7) complex containing FBXO7. Component of the SCF(FBXO10) complex containing FBXO10. Component of the SCF(FBXO11) complex containing FBXO11. Component of the SCF(FBXO25) complex containing FBXO25. Component of the SCF(FBXO33) complex containing FBXO33. Component of the probable SCF(FBXO4) complex containing FBXO4. Component of the SCF(FBXO44) complex, composed of SKP1, CUL1 and FBXO44. Component of the SCF(BTRC) complex, composed of SKP1, CUL1 and BTRC. This complex binds phosphorylated NFKBIA. Part of a SCF complex consisting of CUL1, RBX1, SKP1 and FBXO2. Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Component of the SCF(FBXO17) complex, composed of SKP1, CUL1 and FBXO17. Component of the SCF(FBXO27) complex, composed of SKP1, CUL1 and FBXO27. Component of the SCF(CCNF) complex consisting of CUL1, RBX1, SKP1 and CCNF. Component of the SCF(FBXL3) complex composed of CUL1, SKP1, RBX1 and FBXL3. Component of the SCF(FBXL21) complex composed of CUL1, SKP1, RBX1 and FBXL21. Component of the SCF(FBXO9) composed of CUL1, SKP1, RBX1 and FBXO9. Component of the SCF(FBXW7) composed of CUL1, SKP1, RBX1 and FBXW7. Interacts with CEP68. Interacts with NOTCH2 (By similarity).By similarity

Binary interactionsi

Protein-protein interaction databases

BioGridi252084, 2 interactors
DIPiDIP-29863N
IntActiQ6PEC4, 7 interactors
STRINGi10116.ENSRNOP00000007676

Structurei

3D structure databases

ProteinModelPortaliQ6PEC4
SMRiQ6PEC4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni104 – 163Interaction with the F-box domain of F-box proteinsBy similarityAdd BLAST60

Sequence similaritiesi

Belongs to the SKP1 family.Curated

Phylogenomic databases

eggNOGiKOG1724 Eukaryota
COG5201 LUCA
GeneTreeiENSGT00390000012652
HOGENOMiHOG000172184
HOVERGENiHBG057008
InParanoidiQ6PEC4
KOiK03094
PhylomeDBiQ6PEC4
TreeFamiTF354233

Family and domain databases

InterProiView protein in InterPro
IPR016897 SKP1
IPR001232 SKP1-like
IPR036296 SKP1-like_dim_sf
IPR011333 SKP1/BTB/POZ_sf
IPR016072 Skp1_comp_dimer
IPR016073 Skp1_comp_POZ
PfamiView protein in Pfam
PF01466 Skp1, 1 hit
PF03931 Skp1_POZ, 1 hit
PIRSFiPIRSF028729 E3_ubiquit_lig_SCF_Skp, 1 hit
SMARTiView protein in SMART
SM00512 Skp1, 1 hit
SUPFAMiSSF54695 SSF54695, 1 hit
SSF81382 SSF81382, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q6PEC4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPTIKLQSSD GEIFEVDVEI AKQSVTIKTM LEDLGMDDEG DDDPVPLPNV
60 70 80 90 100
NAAILKKVIQ WCTHHKDDPP PPEDDENKEK RTDDIPVWDQ EFLKVDQGTL
110 120 130 140 150
FELILAANYL DIKGLLDVTC KTVANMIKGK TPEEIRKTFN IKNDFTEEEE
160
AQVRKENQWC EEK
Length:163
Mass (Da):18,672
Last modified:January 23, 2007 - v3
Checksum:iAE184C00BE6556DB
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2K4X8A0A0G2K4X8_RAT
S-phase kinase-associated protein 1
Skp1
165Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC058152 mRNA Translation: AAH58152.1
RefSeqiNP_001007609.1, NM_001007608.2
UniGeneiRn.3477

Genome annotation databases

EnsembliENSRNOT00000007676; ENSRNOP00000007676; ENSRNOG00000005828
GeneIDi287280
KEGGirno:287280
UCSCiRGD:1359648 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC058152 mRNA Translation: AAH58152.1
RefSeqiNP_001007609.1, NM_001007608.2
UniGeneiRn.3477

3D structure databases

ProteinModelPortaliQ6PEC4
SMRiQ6PEC4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi252084, 2 interactors
DIPiDIP-29863N
IntActiQ6PEC4, 7 interactors
STRINGi10116.ENSRNOP00000007676

PTM databases

iPTMnetiQ6PEC4
PhosphoSitePlusiQ6PEC4
SwissPalmiQ6PEC4

Proteomic databases

PaxDbiQ6PEC4
PRIDEiQ6PEC4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007676; ENSRNOP00000007676; ENSRNOG00000005828
GeneIDi287280
KEGGirno:287280
UCSCiRGD:1359648 rat

Organism-specific databases

CTDi6500
RGDi1359648 Skp1

Phylogenomic databases

eggNOGiKOG1724 Eukaryota
COG5201 LUCA
GeneTreeiENSGT00390000012652
HOGENOMiHOG000172184
HOVERGENiHBG057008
InParanoidiQ6PEC4
KOiK03094
PhylomeDBiQ6PEC4
TreeFamiTF354233

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-RNO-1169091 Activation of NF-kappaB in B cells
R-RNO-1170546 Prolactin receptor signaling
R-RNO-174113 SCF-beta-TrCP mediated degradation of Emi1
R-RNO-187577 SCF(Skp2)-mediated degradation of p27/p21
R-RNO-195253 Degradation of beta-catenin by the destruction complex
R-RNO-202424 Downstream TCR signaling
R-RNO-2565942 Regulation of PLK1 Activity at G2/M Transition
R-RNO-2871837 FCERI mediated NF-kB activation
R-RNO-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-RNO-5607764 CLEC7A (Dectin-1) signaling
R-RNO-5610780 Degradation of GLI1 by the proteasome
R-RNO-5610785 GLI3 is processed to GLI3R by the proteasome
R-RNO-5676590 NIK-->noncanonical NF-kB signaling
R-RNO-5684264 MAP3K8 (TPL2)-dependent MAPK1/3 activation
R-RNO-68949 Orc1 removal from chromatin
R-RNO-69231 Cyclin D associated events in G1
R-RNO-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-RNO-8939902 Regulation of RUNX2 expression and activity
R-RNO-8951664 Neddylation
R-RNO-9020702 Interleukin-1 signaling
R-RNO-917937 Iron uptake and transport
R-RNO-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

PROiPR:Q6PEC4

Gene expression databases

BgeeiENSRNOG00000005828 Expressed in 10 organ(s), highest expression level in adult mammalian kidney
ExpressionAtlasiQ6PEC4 baseline and differential
GenevisibleiQ6PEC4 RN

Family and domain databases

InterProiView protein in InterPro
IPR016897 SKP1
IPR001232 SKP1-like
IPR036296 SKP1-like_dim_sf
IPR011333 SKP1/BTB/POZ_sf
IPR016072 Skp1_comp_dimer
IPR016073 Skp1_comp_POZ
PfamiView protein in Pfam
PF01466 Skp1, 1 hit
PF03931 Skp1_POZ, 1 hit
PIRSFiPIRSF028729 E3_ubiquit_lig_SCF_Skp, 1 hit
SMARTiView protein in SMART
SM00512 Skp1, 1 hit
SUPFAMiSSF54695 SSF54695, 1 hit
SSF81382 SSF81382, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSKP1_RAT
AccessioniPrimary (citable) accession number: Q6PEC4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 106 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again