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Protein

Proteasome adapter and scaffold protein ECM29

Gene

Ecpas

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter/scaffolding protein that binds to the 26S proteasome, motor proteins and other compartment specific proteins. May couple the proteasome to different compartments including endosome, endoplasmic reticulum and centrosome. May play a role in ERAD and other enhanced proteolyis (By similarity). Promotes proteasome dissociation under oxidative stress (PubMed:26802743).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome adapter and scaffold protein ECM29By similarity
Alternative name(s):
Proteasome-associated protein ECM29 homolog
Gene namesi
Name:Ecpas
Synonyms:Ecm29, Kiaa0368
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2140220 Ecpas

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endoplasmic reticulum, Endosome, Nucleus, Proteasome

Pathology & Biotechi

Disruption phenotypei

Deficient mice are viable, fertile, and do not show any histological abnormalities. Under stressed condition, 26S proteasome dissociates in wild-type cells, but not in cells from deficient mice.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002125601 – 1840Proteasome adapter and scaffold protein ECM29Add BLAST1840

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei825PhosphoserineBy similarity1
Modified residuei831PhosphothreonineBy similarity1
Cross-linki1034Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6PDI5
PeptideAtlasiQ6PDI5
PRIDEiQ6PDI5

PTM databases

iPTMnetiQ6PDI5
PhosphoSitePlusiQ6PDI5

Expressioni

Tissue specificityi

Widely expressed (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000050812 Expressed in 286 organ(s), highest expression level in quadriceps femoris
CleanExiMM_AI314180
ExpressionAtlasiQ6PDI5 baseline and differential
GenevisibleiQ6PDI5 MM

Interactioni

Subunit structurei

Non-stoichiometric component of the proteasome; associates with the 26S proteasome. Interacts (via N-terminus) with VPS11, VPS26A, VPS36, RAB11FIP4 and RABEP1. Interacts (via C-terminus) with DCTN1, DCTN2, KIF5B, MYH7, MYH10, MYO10 and ARF6.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230950, 7 interactors
IntActiQ6PDI5, 6 interactors
STRINGi10090.ENSMUSP00000099953

Structurei

3D structure databases

ProteinModelPortaliQ6PDI5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati28 – 65HEAT 1Add BLAST38
Repeati107 – 144HEAT 2Add BLAST38
Repeati162 – 199HEAT 3Add BLAST38
Repeati327 – 363HEAT 4Add BLAST37
Repeati388 – 427HEAT 5Add BLAST40
Repeati430 – 467HEAT 6Add BLAST38
Repeati470 – 508HEAT 7Add BLAST39
Repeati722 – 760HEAT 8Add BLAST39
Repeati773 – 810HEAT 9Add BLAST38
Repeati830 – 869HEAT 10Add BLAST40
Repeati871 – 908HEAT 11Add BLAST38
Repeati932 – 970HEAT 12Add BLAST39
Repeati976 – 1013HEAT 13Add BLAST38
Repeati1014 – 1051HEAT 14Add BLAST38
Repeati1113 – 1150HEAT 15Add BLAST38
Repeati1153 – 1190HEAT 16Add BLAST38
Repeati1195 – 1232HEAT 17Add BLAST38
Repeati1244 – 1282HEAT 18Add BLAST39
Repeati1286 – 1324HEAT 19Add BLAST39
Repeati1349 – 1387HEAT 20Add BLAST39
Repeati1391 – 1428HEAT 21Add BLAST38
Repeati1518 – 1555HEAT 22Add BLAST38
Repeati1559 – 1596HEAT 23Add BLAST38
Repeati1659 – 1696HEAT 24Add BLAST38
Repeati1780 – 1823HEAT 25Add BLAST44

Sequence similaritiesi

Belongs to the ECM29 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0915 Eukaryota
ENOG410XPYT LUCA
GeneTreeiENSGT00390000007729
HOGENOMiHOG000231642
HOVERGENiHBG051438
InParanoidiQ6PDI5
KOiK11886
OMAiMKTPAKY
OrthoDBiEOG091G00LP
TreeFamiTF314213

Family and domain databases

Gene3Di1.25.10.10, 2 hits
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR024372 Ecm29
PANTHERiPTHR23346:SF19 PTHR23346:SF19, 1 hit
PfamiView protein in Pfam
PF13001 Ecm29, 1 hit
SUPFAMiSSF48371 SSF48371, 3 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q6PDI5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
METGSDSDQL ERVFLRLGHA ETDEQLQNII SKFLPPVLLK LSSTQEGVRK
60 70 80 90 100
KVMELLVHLN KRIKSRPKIQ LPVETLLVQY QDPAAVSFVT NFTIIYVKMG
110 120 130 140 150
YPRLPVEKQC ELAPTLLTAM EGKPQPQQDS LMHLLIPTLF HMKYPAESSK
160 170 180 190 200
SASPFNLAEK PKTVQLLLDF MLDVLLMPYG YVLNESQSRQ NSSSSSQGSS
210 220 230 240 250
SNSGGGSGIP QPPPGMSFYA AKRVIGDNPW TPEQLEQCKL GIVKFIEAEQ
260 270 280 290 300
VPELEAVLHL VIASSDTRHS VATAADLELK SKQSLIDWNN PAIINKMYKV
310 320 330 340 350
YLGDIPLKTK EGAVLKPELK RDPVSTRVKL KIVPHLLRSR QAAETFPANI
360 370 380 390 400
QVVYDGLFGT NTNSKLRTLS LQFVHHICLT CPEIKIKPLG PMLLNGLTKL
410 420 430 440 450
INEYKEDPKL LSMAYSAVGK LSSRMPHLFT KDIALVQQLF EALCKEEPET
460 470 480 490 500
RLAIQEALSM MVGAYSTLEG AQRTLMEALV ASYLIKPEVQ VRQVAVKFAS
510 520 530 540 550
TVFPSDHIPS RYLLLLAAGD PREEVHGEAQ RVLRCLPGRN KKESASKQMP
560 570 580 590 600
SFPEMVYYIQ EKASHRMKTP VKYMTGTTVL PFNPAAFGEI VLYLRMCLAH
610 620 630 640 650
SAGVVPTSQS LADMQDHAPA IGRYIRALMS SSQATASSSN KSGETNPVQI
660 670 680 690 700
YTGLLQQLLA GVGGLPVMYC LLEAVSVYPE KLATKFVDKT EWIKSLMSSS
710 720 730 740 750
KEEMRELAAL FYSVVVSTVS GIELKSMIEQ LIKATKDNHS PEVQHGSLLA
760 770 780 790 800
LGFTVGRYLA KKRVRMAEQH DLETDADLLP EQEEIIRSAT ETIGSFLDST
810 820 830 840 850
SPLLAIAACT ALGEIGRNGP LPIPSEGSGF TKLHLVESLL NRIPSSKETN
860 870 880 890 900
KMKERAIQTL GYFPVGDGVF PHQKLLLQGL MDSVEAKQIE LQFTIGEAIT
910 920 930 940 950
SAAIGTNSVA ARDAWLVTEE EYIPPAGAKV NDVVPWVLDV ILNKHIISPN
960 970 980 990 1000
PHVRQAACIW LLSLVRKLST HREVKSHLKE IQSAFVSVLS ENDELSQDVA
1010 1020 1030 1040 1050
SKGLGLVYEL GNEQDQQELV STLVETLMTG KRVKHEVSGE TVVFQGGGLG
1060 1070 1080 1090 1100
KTPDGQGLST YKELCSLASD LSQPDLVYKF MNLANHHAMW NSRKGAAFGF
1110 1120 1130 1140 1150
NVIATRAGEQ LAPFLPQLVP RLYRYQFDPN LGIRQAMTSI WNALVTDKSM
1160 1170 1180 1190 1200
VDKYLKEILQ DLIKNLTSNM WRVRESSCLA LNDLLRGRPL DDVIDKLPEM
1210 1220 1230 1240 1250
WETLFRVQDD IKESVRKAAE LALKTLSKVC VKMCDPAKGA AGQRTIAVLL
1260 1270 1280 1290 1300
PCLLDKGMMS PVTEVRALSI NTLVKISKSA GAMLKPHAPK LIPALLESLS
1310 1320 1330 1340 1350
VLEPQVLNYL SLRATEQEKD VMDSARLSAA KSSPMMETIN MCLQYLDVSV
1360 1370 1380 1390 1400
LGELVPRLCE LIRSGVGLGT KGGCASVIVS LTTQCPQDLT PYSGKLMSAL
1410 1420 1430 1440 1450
LSGLTDRNSV IQKSCAFAMG HLVRTSRDSS TEKLLQKLNG WYMEKDEPVY
1460 1470 1480 1490 1500
KTSCALTIHA IGRYSPDVLK NHAKEVLPLA FLGMHEIADE EKSEKEECNM
1510 1520 1530 1540 1550
WTEVWQENVP GSFGGIRLYL QELITITQKA LQSQSWKMKA QGAIAMASIS
1560 1570 1580 1590 1600
KQTSSLVPPY LGMILSALMQ GLAGRTWAGK EELLKAIACV VTACSTELEK
1610 1620 1630 1640 1650
SVPNQPTTNE ILQAVLKECC KENLKYKIVA ISCAADVLKA TKEDRFQEFS
1660 1670 1680 1690 1700
DIVIPLIKKN SLESMGVRTT KAEDENEKER ELQLESLLGA FESLGKAWPR
1710 1720 1730 1740 1750
NPDTQRCYRQ ELCKLMCERL RLSTWKVQLG VLQSMNAFFQ GLMLLEEEHA
1760 1770 1780 1790 1800
DPEALAEILL ETCKSITYSL ENKTYSSVRT EALSVVELLL KKLEEAKQWE
1810 1820 1830 1840
SLTAECRGLL IESLATMETD NRPELQEKAS VLKKTLESLE
Length:1,840
Mass (Da):203,703
Last modified:July 27, 2011 - v3
Checksum:i7FCB7FFDBFD1539D
GO
Isoform 2 (identifier: Q6PDI5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: METGSDS → MYHIDCR

Show »
Length:1,840
Mass (Da):203,915
Checksum:i447D913AA8B0C19E
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2ALV6A2ALV6_MOUSE
Proteasome adapter and scaffold pro...
Ecpas
942Annotation score:
A2ALV7A2ALV7_MOUSE
Proteasome adapter and scaffold pro...
Ecpas
1,490Annotation score:
A2ALW1A2ALW1_MOUSE
Proteasome adapter and scaffold pro...
Ecpas
361Annotation score:
A2ALV9A2ALV9_MOUSE
Proteasome adapter and scaffold pro...
Ecpas
246Annotation score:
A2ALV8A2ALV8_MOUSE
Proteasome adapter and scaffold pro...
Ecpas
538Annotation score:

Sequence cautioni

The sequence BAC30129 differs from that shown. Intron retention.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1446D → E in AAH25035 (PubMed:15489334).Curated1
Sequence conflicti1446D → E in BAC97937 (PubMed:14621295).Curated1
Sequence conflicti1449V → F in BAC26926 (PubMed:14621295).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0131781 – 7METGSDS → MYHIDCR in isoform 2. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL805972, AL806535, AL929394 Genomic DNA Translation: CAM17649.1
AL929394, AL805972, AL806535 Genomic DNA Translation: CAM22037.1
AL806535, AL805972, AL929394 Genomic DNA Translation: CAM24807.1
BC025035 mRNA Translation: AAH25035.1
BC058684 mRNA Translation: AAH58684.1
AK030369 mRNA Translation: BAC26926.1
AK038775 mRNA Translation: BAC30129.1 Sequence problems.
AK129127 mRNA Translation: BAC97937.1
CCDSiCCDS18214.1 [Q6PDI5-1]
RefSeqiNP_759013.2, NM_172381.2 [Q6PDI5-1]
XP_006537911.1, XM_006537848.3 [Q6PDI5-2]
XP_006537912.1, XM_006537849.3
UniGeneiMm.234801
Mm.441740

Genome annotation databases

EnsembliENSMUST00000102889; ENSMUSP00000099953; ENSMUSG00000050812 [Q6PDI5-1]
GeneIDi230249
KEGGimmu:230249
UCSCiuc008sze.1 mouse [Q6PDI5-1]
uc008szf.1 mouse [Q6PDI5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL805972, AL806535, AL929394 Genomic DNA Translation: CAM17649.1
AL929394, AL805972, AL806535 Genomic DNA Translation: CAM22037.1
AL806535, AL805972, AL929394 Genomic DNA Translation: CAM24807.1
BC025035 mRNA Translation: AAH25035.1
BC058684 mRNA Translation: AAH58684.1
AK030369 mRNA Translation: BAC26926.1
AK038775 mRNA Translation: BAC30129.1 Sequence problems.
AK129127 mRNA Translation: BAC97937.1
CCDSiCCDS18214.1 [Q6PDI5-1]
RefSeqiNP_759013.2, NM_172381.2 [Q6PDI5-1]
XP_006537911.1, XM_006537848.3 [Q6PDI5-2]
XP_006537912.1, XM_006537849.3
UniGeneiMm.234801
Mm.441740

3D structure databases

ProteinModelPortaliQ6PDI5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230950, 7 interactors
IntActiQ6PDI5, 6 interactors
STRINGi10090.ENSMUSP00000099953

PTM databases

iPTMnetiQ6PDI5
PhosphoSitePlusiQ6PDI5

Proteomic databases

PaxDbiQ6PDI5
PeptideAtlasiQ6PDI5
PRIDEiQ6PDI5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102889; ENSMUSP00000099953; ENSMUSG00000050812 [Q6PDI5-1]
GeneIDi230249
KEGGimmu:230249
UCSCiuc008sze.1 mouse [Q6PDI5-1]
uc008szf.1 mouse [Q6PDI5-2]

Organism-specific databases

CTDi23392
MGIiMGI:2140220 Ecpas
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0915 Eukaryota
ENOG410XPYT LUCA
GeneTreeiENSGT00390000007729
HOGENOMiHOG000231642
HOVERGENiHBG051438
InParanoidiQ6PDI5
KOiK11886
OMAiMKTPAKY
OrthoDBiEOG091G00LP
TreeFamiTF314213

Miscellaneous databases

PROiPR:Q6PDI5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000050812 Expressed in 286 organ(s), highest expression level in quadriceps femoris
CleanExiMM_AI314180
ExpressionAtlasiQ6PDI5 baseline and differential
GenevisibleiQ6PDI5 MM

Family and domain databases

Gene3Di1.25.10.10, 2 hits
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR024372 Ecm29
PANTHERiPTHR23346:SF19 PTHR23346:SF19, 1 hit
PfamiView protein in Pfam
PF13001 Ecm29, 1 hit
SUPFAMiSSF48371 SSF48371, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiECM29_MOUSE
AccessioniPrimary (citable) accession number: Q6PDI5
Secondary accession number(s): A2ALW0
, Q6ZQC9, Q8BSW7, Q8CAH0, Q8R3M6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 126 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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