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Protein

Trafficking kinesin-binding protein 1

Gene

Trak1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the regulation of endosome-to-lysosome trafficking, including endocytic trafficking of EGF-EGFR complexes and GABA-A receptors (By similarity). Involved in mitochondrial motility (PubMed:24995978). When O-glycosylated, abolishes mitochondrial motility. Crucial for recruiting OGT to the mitochondrial surface of neuronal processes (By similarity). TRAK1 and RHOT form an essential protein complex that links KIF5 to mitochondria for light chain-independent, anterograde transport of mitochondria (By similarity).By similarity1 Publication

GO - Molecular functioni

  • GABA receptor binding Source: MGI
  • myosin binding Source: GO_Central
  • signaling receptor binding Source: GO_Central
  • TPR domain binding Source: MGI

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Trafficking kinesin-binding protein 1
Alternative name(s):
Protein Milton1 Publication
Gene namesi
Name:Trak1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1914345 Trak1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane, Mitochondrion, Nucleus

Pathology & Biotechi

Involvement in diseasei

A spontaneous mutation (hyrt mice) causes a recessively transmitted form of hypertonia neurological dysfunction characterized by postural abnormalities, jerky movements and tremormutant. Hyrt mice have much lower levels of GABA(A) receptors in the CNS, particularly the lower motor neurons, than do wild-type mice, indicating that the hypertonicity of the mutants is likely to be caused by deficits in GABA-mediated motor neuron inhibition.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003945061 – 939Trafficking kinesin-binding protein 1Add BLAST939

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi444O-linked (GlcNAc) serineBy similarity1
Modified residuei534PhosphoserineCombined sources1
Glycosylationi677O-linked (GlcNAc) serineBy similarity1
Modified residuei716PhosphoserineCombined sources1
Glycosylationi716O-linked (GlcNAc) serineBy similarity1
Modified residuei905PhosphoserineCombined sources1

Post-translational modificationi

O-glycosylated (PubMed:24995978). Glycosylated by OGT; glycosylation in response to increased extracellular glucose levels is required for and leads to regulation of mitochondrial motility by OGT (By similarity).By similarity1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ6PD31
PRIDEiQ6PD31

PTM databases

iPTMnetiQ6PD31
PhosphoSitePlusiQ6PD31

Expressioni

Tissue specificityi

Widely expressed with the greatest expression in brain, liver and kidney. Detected throughout the CNS, including the cortex, hippocamps, thalamus and various subcortical nuclei of the forebrain and midbrain, the granule of Purkinje layers of the cerebellum and the gray matter of the spinal cord. High level detected in lower moter neurons (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000032536 Expressed in 286 organ(s), highest expression level in aortic valve
ExpressionAtlasiQ6PD31 baseline and differential
GenevisibleiQ6PD31 MM

Interactioni

Subunit structurei

Interacts with RHOT1 and RHOT2. Found in a complex with KIF5B, OGT, RHOT1 and RHOT2. Interacts with HGS (By similarity). Interacts with GABRA1 (PubMed:16380713). Interacts with KIF5C. Interacts with OGT; stable interaction is not required for glycosylation of this protein by OGT. Isoform 1 interacts with OGT (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044482

Structurei

3D structure databases

ProteinModelPortaliQ6PD31
SMRiQ6PD31
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 353HAP1 N-terminalAdd BLAST308

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni359 – 509Interaction with HGSBy similarityAdd BLAST151
Regioni655 – 669Interaction with OGTBy similarityAdd BLAST15

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili106 – 354Sequence analysisAdd BLAST249
Coiled coili490 – 524Sequence analysisAdd BLAST35

Sequence similaritiesi

Belongs to the milton family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4360 Eukaryota
ENOG410XSWD LUCA
GeneTreeiENSGT00390000003132
HOVERGENiHBG069248
InParanoidiQ6PD31
KOiK15369
OMAiQRKVNTL
OrthoDBiEOG091G0LLD
PhylomeDBiQ6PD31
TreeFamiTF323495

Family and domain databases

InterProiView protein in InterPro
IPR006933 HAP1_N
IPR022154 Traffickng_kinesin-bd_prot_dom
PfamiView protein in Pfam
PF04849 HAP1_N, 1 hit
PF12448 Milton, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q6PD31-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MALAIQLRQP SRAQPLPGLS HTLAGTDSCD VCNSTNLPEV EIISLLEEQL
60 70 80 90 100
PHYKLRADTI YGYDHDDWLH TPLISPDANI DLTTEQIEET LKYFLLCAER
110 120 130 140 150
VGQMTKTYND IDAVTRLLEE KERDLELAAR IGQSLLKKNK TLTERNELLE
160 170 180 190 200
EQVEHIREEV SQLRHELSMK DELLQFYTSA AEESEPESVC STPLKRNESS
210 220 230 240 250
SSVQNYFHLD SLQKKLKDLE EENVVLRSEA CQLKTETITY EEKEQQLVND
260 270 280 290 300
CVKELRDANV QIASISEELA KKTEDAARQQ EEITHLLSQI VDLQKKAKSC
310 320 330 340 350
AVENEELVQH LGAAKDAQRQ LTAELRELED KYAECMEMLH EAQEELKNLR
360 370 380 390 400
NKTMPTSRRY HSLGLFPMDS LAAEIEGTMR KELQLEELES PDITHQKRVF
410 420 430 440 450
ETVRNVNQVV KQRSLTPSPM NIPGSNQSSA MNSLLSSCVS TPRSSFYGSD
460 470 480 490 500
VSNVVLDNKT NSILLETEAA DLGNEDHNKK PGTPGTPGSH DLETALRRLS
510 520 530 540 550
LRRENYLSER RFFEEEQERK LRELAEKGEL HSGSLTPTES IMSLGTHSRF
560 570 580 590 600
SEFTGFSGMS FSSRSYLPEK LQIVKPLEGS ATLHHWQQLA QPHLGGILDP
610 620 630 640 650
RPGVVTKGFR TLDVDLDEVY CLNDFEEDDT GDHISLAGLA TSTPIQHPET
660 670 680 690 700
SAHHPGKCMS QTNSTFTFTT CRILHPSDEL TRVTPSLNSA PAPACSSTSH
710 720 730 740 750
LKSTPVATPC TPRRLSLAES FTNVRESTTT MSTSLGLVWL LKERGISAAV
760 770 780 790 800
YDPQSWDRAG RGSLLHSYTP RMAVIPSTPP NSPMQTPSAS PPSFEFKCTS
810 820 830 840 850
PPYNNFLASK PASSILREVR EKRPVRSSES QTDVSVSNLN LVDKVRRFGV
860 870 880 890 900
ARVVNSGRAR IPTLTEEQGP LLCGPTGPAQ ALVPGGLVPE GLPLGCPSGI
910 920 930
RRNRSFPTMV GSSVQMRAPV ILTSGILMGA KLPKQTSLR
Length:939
Mass (Da):104,467
Last modified:July 5, 2004 - v1
Checksum:i64B1D5D34DF36FCB
GO
Isoform 2 (identifier: Q6PD31-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-35: MALAIQLRQPSRAQPLPGLSHTLAGTDSCDVCNST → MLPATESTGMPAPSPTCATAP
     580-627: SATLHHWQQL...EVYCLNDFEE → DHQGLSVLLC...SHPRCWFEFL
     628-939: Missing.

Show »
Length:610
Mass (Da):69,613
Checksum:iF4098F77CEA7E335
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q80VE1Q80VE1_MOUSE
Trafficking kinesin-binding protein...
Trak1
256Annotation score:
A0A1B0GRQ7A0A1B0GRQ7_MOUSE
Trafficking kinesin-binding protein...
Trak1
731Annotation score:
A0A1B0GRL4A0A1B0GRL4_MOUSE
Trafficking kinesin-binding protein...
Trak1
241Annotation score:
A0A1B0GRP1A0A1B0GRP1_MOUSE
Trafficking kinesin-binding protein...
Trak1
560Annotation score:
A0A1B0GRS7A0A1B0GRS7_MOUSE
Trafficking kinesin-binding protein...
Trak1
606Annotation score:
A0A1B0GSQ2A0A1B0GSQ2_MOUSE
Trafficking kinesin-binding protein...
Trak1
524Annotation score:
A0A1B0GSP3A0A1B0GSP3_MOUSE
Trafficking kinesin-binding protein...
Trak1
234Annotation score:
A0A1B0GSC9A0A1B0GSC9_MOUSE
Trafficking kinesin-binding protein...
Trak1
24Annotation score:
A0A1B0GS60A0A1B0GS60_MOUSE
Trafficking kinesin-binding protein...
Trak1
54Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0392771 – 35MALAI…VCNST → MLPATESTGMPAPSPTCATA P in isoform 2. 1 PublicationAdd BLAST35
Alternative sequenceiVSP_039278580 – 627SATLH…NDFEE → DHQGLSVLLCDSLWALIHHR KASHQCHTYSFFFRDSHPRC WFEFL in isoform 2. 1 PublicationAdd BLAST48
Alternative sequenceiVSP_039279628 – 939Missing in isoform 2. 1 PublicationAdd BLAST312

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041436 mRNA Translation: BAC30946.1
BC058971 mRNA Translation: AAH58971.1
CH466587 Genomic DNA Translation: EDL09157.1
CCDSiCCDS40810.1 [Q6PD31-1]
RefSeqiNP_780323.2, NM_175114.3 [Q6PD31-1]
UniGeneiMm.491112

Genome annotation databases

EnsembliENSMUST00000045903; ENSMUSP00000044482; ENSMUSG00000032536 [Q6PD31-1]
GeneIDi67095
KEGGimmu:67095
UCSCiuc009sdd.1 mouse [Q6PD31-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041436 mRNA Translation: BAC30946.1
BC058971 mRNA Translation: AAH58971.1
CH466587 Genomic DNA Translation: EDL09157.1
CCDSiCCDS40810.1 [Q6PD31-1]
RefSeqiNP_780323.2, NM_175114.3 [Q6PD31-1]
UniGeneiMm.491112

3D structure databases

ProteinModelPortaliQ6PD31
SMRiQ6PD31
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044482

PTM databases

iPTMnetiQ6PD31
PhosphoSitePlusiQ6PD31

Proteomic databases

PaxDbiQ6PD31
PRIDEiQ6PD31

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045903; ENSMUSP00000044482; ENSMUSG00000032536 [Q6PD31-1]
GeneIDi67095
KEGGimmu:67095
UCSCiuc009sdd.1 mouse [Q6PD31-1]

Organism-specific databases

CTDi22906
MGIiMGI:1914345 Trak1

Phylogenomic databases

eggNOGiKOG4360 Eukaryota
ENOG410XSWD LUCA
GeneTreeiENSGT00390000003132
HOVERGENiHBG069248
InParanoidiQ6PD31
KOiK15369
OMAiQRKVNTL
OrthoDBiEOG091G0LLD
PhylomeDBiQ6PD31
TreeFamiTF323495

Miscellaneous databases

ChiTaRSiTrak1 mouse
PROiPR:Q6PD31
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032536 Expressed in 286 organ(s), highest expression level in aortic valve
ExpressionAtlasiQ6PD31 baseline and differential
GenevisibleiQ6PD31 MM

Family and domain databases

InterProiView protein in InterPro
IPR006933 HAP1_N
IPR022154 Traffickng_kinesin-bd_prot_dom
PfamiView protein in Pfam
PF04849 HAP1_N, 1 hit
PF12448 Milton, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRAK1_MOUSE
AccessioniPrimary (citable) accession number: Q6PD31
Secondary accession number(s): Q8BYA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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