UniProtKB - Q6PAK3 (ANM8_MOUSE)
Protein arginine N-methyltransferase 8
Prmt8
Functioni
S-adenosyl-L-methionine-dependent and membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA) in proteins such as NIFK, myelin basic protein, histone H4, H2A and H2A/H2B dimer. Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS.
By similarityCatalytic activityi
- L-arginyl-[protein] + S-adenosyl-L-methionine = H+ + Nω-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- L-arginyl-[protein] + 2 S-adenosyl-L-methionine = 2 H+ + Nω,Nω-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteineBy similarityEC:2.1.1.319By similarityThis reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 86 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 95 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 119 | S-adenosyl-L-methionine; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 141 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 170 | S-adenosyl-L-methionineBy similarity | 1 | |
Active sitei | 185 | By similarity | 1 | |
Active sitei | 194 | By similarity | 1 |
GO - Molecular functioni
- enzyme binding Source: HGNC-UCL
- histone-arginine N-methyltransferase activity Source: HGNC-UCL
- identical protein binding Source: UniProtKB
- protein-arginine omega-N asymmetric methyltransferase activity Source: UniProtKB
- protein-arginine omega-N monomethyltransferase activity Source: HGNC-UCL
- protein homodimerization activity Source: HGNC-UCL
- S-adenosyl-L-methionine binding Source: UniProtKB
- S-adenosylmethionine-dependent methyltransferase activity Source: HGNC-UCL
GO - Biological processi
- histone methylation Source: HGNC-UCL
- peptidyl-arginine methylation Source: HGNC-UCL
- peptidyl-arginine methylation, to asymmetrical-dimethyl arginine Source: HGNC-UCL
- protein homooligomerization Source: UniProtKB
- protein methylation Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: Protein arginine N-methyltransferase 8Curated (EC:2.1.1.319By similarity)Alternative name(s): Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3043083, Prmt8 |
VEuPathDBi | HostDB:ENSMUSG00000030350 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
Plasma Membrane
- anchored component of the cytoplasmic side of the plasma membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000212330 | 2 – 394 | Protein arginine N-methyltransferase 8Add BLAST | 393 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Modified residuei | 58 | Omega-N-methylarginine; by PRMT8By similarity | 1 | |
Modified residuei | 73 | Asymmetric dimethylarginine; by PRMT8By similarity | 1 |
Keywords - PTMi
Lipoprotein, Methylation, MyristateProteomic databases
MaxQBi | Q6PAK3 |
PaxDbi | Q6PAK3 |
PeptideAtlasi | Q6PAK3 |
PRIDEi | Q6PAK3 |
ProteomicsDBi | 296046 |
PTM databases
PhosphoSitePlusi | Q6PAK3 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000030350, Expressed in Ammon's horn and 127 other tissues |
Genevisiblei | Q6PAK3, MM |
Interactioni
Subunit structurei
Homodimer. Tetramer; individual homodimers associates to form a homotetramer. Homooctamer; individual homodimers associates to form a homooctamer and homooligomerization is required for proper localization to the cell membrane. Heterodimer with PRMT1; heterodimerization may recruit PRMT1 activity to the plasma membrane.
Interacts with PRMT2 (via the SH3 domain).
Interacts with FYN (via the SH3 domain).
Interacts with EWS; independently of EWS methylation status.
By similarityGO - Molecular functioni
- enzyme binding Source: HGNC-UCL
- identical protein binding Source: UniProtKB
- protein homodimerization activity Source: HGNC-UCL
Protein-protein interaction databases
BioGRIDi | 238106, 20 interactors |
IntActi | Q6PAK3, 2 interactors |
STRINGi | 10090.ENSMUSP00000032500 |
Miscellaneous databases
RNActi | Q6PAK3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 73 – 394 | SAM-dependent MTase PRMT-typePROSITE-ProRule annotationAdd BLAST | 322 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 21 – 40 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 119 – 122 | S-adenosyl-L-methionine bindingBy similarity | 4 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 29 – 42 | SH3-binding 1By similarityAdd BLAST | 14 | |
Motifi | 53 – 58 | SH3-binding 2By similarity | 6 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1499, Eukaryota |
GeneTreei | ENSGT00940000155867 |
HOGENOMi | CLU_017375_1_1_1 |
InParanoidi | Q6PAK3 |
OMAi | RNDFVHA |
OrthoDBi | 840669at2759 |
PhylomeDBi | Q6PAK3 |
TreeFami | TF300608 |
Family and domain databases
Gene3Di | 3.40.50.150, 1 hit |
InterProi | View protein in InterPro IPR025799, Arg_MeTrfase IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR11006, PTHR11006, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51678, SAM_MT_PRMT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGMKHSSRCL LLRRKMAENA VESTEVSSAP PQPPQPVIPA KPVQCVHHVS
60 70 80 90 100
TQPSCPGRGK MSKLLNPEEM TSRDYYFDSY AHFGIHEEML KDEVRTLTYR
110 120 130 140 150
NSMYHNKHVF KDKVVLDVGS GTGILSMFAA KAGAKKVFGI ECSSISDYSE
160 170 180 190 200
KIIKANHLDN VITIFKGKVE EVELPVEKVD IIISEWMGYC LFYESMLNTV
210 220 230 240 250
IFARDKWLKP GGLMFPDRAA LYVVAIEDRQ YKDFKIHWWE NVYGFDMTCI
260 270 280 290 300
RDVAMKEPLV DIVDPKQVVT NACLIKEVDI YTVKTEELSF TSAFCLQIQR
310 320 330 340 350
NDYVHALVTY FNIEFTKCHK KMGFSTAPDA PYTHWKQTVF YLEDYLTVRR
360 370 380 390
GEEIYGTISM KPNAKNVRDL DFTVDLDFKG QLCETSVSND YKMR
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC127373 Genomic DNA No translation available. BC060250 mRNA Translation: AAH60250.1 Different initiation. BK001349 mRNA Translation: DAA01382.1 |
CCDSi | CCDS57449.1 |
RefSeqi | NP_958759.2, NM_201371.2 |
Genome annotation databases
Ensembli | ENSMUST00000032500; ENSMUSP00000032500; ENSMUSG00000030350 |
GeneIDi | 381813 |
KEGGi | mmu:381813 |
UCSCi | uc009dwb.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC127373 Genomic DNA No translation available. BC060250 mRNA Translation: AAH60250.1 Different initiation. BK001349 mRNA Translation: DAA01382.1 |
CCDSi | CCDS57449.1 |
RefSeqi | NP_958759.2, NM_201371.2 |
3D structure databases
AlphaFoldDBi | Q6PAK3 |
SMRi | Q6PAK3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 238106, 20 interactors |
IntActi | Q6PAK3, 2 interactors |
STRINGi | 10090.ENSMUSP00000032500 |
PTM databases
PhosphoSitePlusi | Q6PAK3 |
Proteomic databases
MaxQBi | Q6PAK3 |
PaxDbi | Q6PAK3 |
PeptideAtlasi | Q6PAK3 |
PRIDEi | Q6PAK3 |
ProteomicsDBi | 296046 |
Protocols and materials databases
Antibodypediai | 10506, 169 antibodies from 27 providers |
DNASUi | 381813 |
Genome annotation databases
Ensembli | ENSMUST00000032500; ENSMUSP00000032500; ENSMUSG00000030350 |
GeneIDi | 381813 |
KEGGi | mmu:381813 |
UCSCi | uc009dwb.2, mouse |
Organism-specific databases
CTDi | 56341 |
MGIi | MGI:3043083, Prmt8 |
VEuPathDBi | HostDB:ENSMUSG00000030350 |
Phylogenomic databases
eggNOGi | KOG1499, Eukaryota |
GeneTreei | ENSGT00940000155867 |
HOGENOMi | CLU_017375_1_1_1 |
InParanoidi | Q6PAK3 |
OMAi | RNDFVHA |
OrthoDBi | 840669at2759 |
PhylomeDBi | Q6PAK3 |
TreeFami | TF300608 |
Miscellaneous databases
BioGRID-ORCSi | 381813, 1 hit in 77 CRISPR screens |
ChiTaRSi | Prmt8, mouse |
PROi | PR:Q6PAK3 |
RNActi | Q6PAK3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030350, Expressed in Ammon's horn and 127 other tissues |
Genevisiblei | Q6PAK3, MM |
Family and domain databases
Gene3Di | 3.40.50.150, 1 hit |
InterProi | View protein in InterPro IPR025799, Arg_MeTrfase IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR11006, PTHR11006, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51678, SAM_MT_PRMT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ANM8_MOUSE | |
Accessioni | Q6PAK3Primary (citable) accession number: Q6PAK3 Secondary accession number(s): Q7M6Z2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | May 25, 2022 | |
This is version 132 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families