UniProtKB - Q6P9S7 (GLT10_MOUSE)
Protein
Polypeptide N-acetylgalactosaminyltransferase 10
Gene
Galnt10
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward Muc5Ac and EA2 peptide substrates (By similarity).By similarity
Caution
According to experiments made in rat, this enzyme is unable to transfer GalNAc onto serine or threonine residue on the protein receptor, but instead requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties, thereby acting as a glycopeptide transferase.Curated
Catalytic activityi
- L-seryl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-seryl-[protein] + H+ + UDPEC:2.4.1.41
- L-threonyl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-threonyl-[protein] + H+ + UDPEC:2.4.1.41
Cofactori
Mn2+By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 185 | SubstrateBy similarity | 1 | |
Binding sitei | 214 | SubstrateBy similarity | 1 | |
Metal bindingi | 237 | ManganeseBy similarity | 1 | |
Binding sitei | 238 | SubstrateBy similarity | 1 | |
Metal bindingi | 239 | ManganeseBy similarity | 1 | |
Binding sitei | 342 | SubstrateBy similarity | 1 | |
Metal bindingi | 370 | ManganeseBy similarity | 1 | |
Binding sitei | 373 | SubstrateBy similarity | 1 | |
Binding sitei | 378 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- polypeptide N-acetylgalactosaminyltransferase activity Source: MGI
GO - Biological processi
- O-glycan processing Source: MGI
- protein O-linked glycosylation Source: MGI
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Lectin, Manganese, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-913709 O-linked glycosylation of mucins |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | CBM13 Carbohydrate-Binding Module Family 13 GT27 Glycosyltransferase Family 27 |
Names & Taxonomyi
Protein namesi | Recommended name: Polypeptide N-acetylgalactosaminyltransferase 10 (EC:2.4.1.41)Alternative name(s): Polypeptide GalNAc transferase 10 Short name: GalNAc-T10 Short name: pp-GaNTase 10 Protein-UDP acetylgalactosaminyltransferase 10 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10 |
Gene namesi | Name:Galnt10 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1890480 Galnt10 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity
Golgi apparatus
- Golgi apparatus Source: GO_Central
- Golgi membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 12 – 31 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 20 | |
Topological domaini | 32 – 603 | LumenalSequence analysisAdd BLAST | 572 |
Keywords - Cellular componenti
Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000059123 | 1 – 603 | Polypeptide N-acetylgalactosaminyltransferase 10Add BLAST | 603 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 124 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 135 ↔ 365 | PROSITE-ProRule annotation | ||
Glycosylationi | 146 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 356 ↔ 432 | PROSITE-ProRule annotation | ||
Disulfide bondi | 471 ↔ 488 | PROSITE-ProRule annotation | ||
Disulfide bondi | 523 ↔ 538 | PROSITE-ProRule annotation | ||
Disulfide bondi | 563 ↔ 578 | PROSITE-ProRule annotation | ||
Glycosylationi | 593 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | Q6P9S7 |
PaxDbi | Q6P9S7 |
PRIDEi | Q6P9S7 |
PTM databases
PhosphoSitePlusi | Q6P9S7 |
SwissPalmi | Q6P9S7 |
Expressioni
Tissue specificityi
Expressed at higher level than GALNT9. In the developing hindbrain region of E14.5 embryos it accumulates in the rapidly dividing, undifferentiated ventricular zone adjacent to the pons. It also accumulates in the regions immediately rostral and caudal to the dorsal rhombic lips differentiating into the cerebellum. Not expressed in the developing choroid plexus.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000020520 Expressed in 256 organ(s), highest expression level in entire extraembryonic component |
ExpressionAtlasi | Q6P9S7 baseline and differential |
Genevisiblei | Q6P9S7 MM |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 458 – 590 | Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST | 133 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 144 – 253 | Catalytic subdomain AAdd BLAST | 110 | |
Regioni | 311 – 373 | Catalytic subdomain BAdd BLAST | 63 | |
Regioni | 373 – 384 | Flexible loopBy similarityAdd BLAST | 12 |
Domaini
There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3736 Eukaryota ENOG410XPMK LUCA |
GeneTreei | ENSGT00940000156690 |
HOGENOMi | HOG000038227 |
InParanoidi | Q6P9S7 |
KOi | K00710 |
OMAi | MRSVHSL |
OrthoDBi | 276134at2759 |
PhylomeDBi | Q6P9S7 |
TreeFami | TF313267 |
Family and domain databases
CDDi | cd00161 RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR001173 Glyco_trans_2-like IPR029044 Nucleotide-diphossugar_trans IPR035992 Ricin_B-like_lectins IPR000772 Ricin_B_lectin |
Pfami | View protein in Pfam PF00535 Glycos_transf_2, 1 hit PF00652 Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458 RICIN, 1 hit |
SUPFAMi | SSF50370 SSF50370, 1 hit SSF53448 SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231 RICIN_B_LECTIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q6P9S7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRRKEKRLLQ AVALALAALV LLPNVGLWAL YRERQPDGSP GGLGAAVAPA
60 70 80 90 100
AVQELHSRQK KTFFLGAEQR LKDWHNKEAI RRDAQRVGYG EQGKPYPMTD
110 120 130 140 150
AERVDQAYRE NGFNIYVSDK ISLNRSLPDI RHPNCNSKLY LETLPNTSII
160 170 180 190 200
IPFHNEGWSS LLRTVHSVLN RSPPELVAEI VLVDDFSDRE HLKKPLEDYM
210 220 230 240 250
ALFPSVRILR TKKREGLIRT RMLGASAATG DVVTFLDSHC EANVNWLPPL
260 270 280 290 300
LDRIARNRKT IVCPMIDVID HDDFRYETQA GDAMRGAFDW EMYYKRIPIP
310 320 330 340 350
PELQKADPSD PFESPVMAGG LFAVDRKWFW ELGGYDPGLE IWGGEQYEIS
360 370 380 390 400
FKVWMCGGRM EDIPCSRVGH IYRKYVPYKV PAGVSLARNL KRVAEVWMDE
410 420 430 440 450
YAEYIYQRRP EYRHLSAGDV VAQKKLRVSL NCKSFKWFMT KIAWDLPKFY
460 470 480 490 500
PPVEPPAAAW GEIRNVGTGL CTDTKLGTLG SPLRLETCIR GRGEAAWNSM
510 520 530 540 550
QVFTFTWRED IRPGDPQHTK KFCFDAVSHT SPVTLYDCHS MKGNQLWKYR
560 570 580 590 600
KDKTLYHPVS GSCMDCSESD HRVFMNTCNP SSLTQQWLFE HTNSTVLENF
NKN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ5ST18 | Q5ST18_MOUSE | Polypeptide N-acetylgalactosaminylt... | Galnt10 | 134 | Annotation score: |
Sequence cautioni
The sequence AAH16585 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAD21405 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 233 | V → I in BAD21405 (PubMed:15449545).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK131155 mRNA Translation: BAD21405.1 Different initiation. BC016585 mRNA Translation: AAH16585.1 Different initiation. BC060617 mRNA Translation: AAH60617.1 AK033515 mRNA Translation: BAC28334.1 |
CCDSi | CCDS24719.1 |
RefSeqi | NP_598950.2, NM_134189.2 |
Genome annotation databases
Ensembli | ENSMUST00000066987; ENSMUSP00000065096; ENSMUSG00000020520 |
GeneIDi | 171212 |
KEGGi | mmu:171212 |
UCSCi | uc007jab.1 mouse |
Similar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase Polypeptide N-acetylgalactosaminyltransferase 10 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK131155 mRNA Translation: BAD21405.1 Different initiation. BC016585 mRNA Translation: AAH16585.1 Different initiation. BC060617 mRNA Translation: AAH60617.1 AK033515 mRNA Translation: BAC28334.1 |
CCDSi | CCDS24719.1 |
RefSeqi | NP_598950.2, NM_134189.2 |
3D structure databases
SMRi | Q6P9S7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000065096 |
Protein family/group databases
CAZyi | CBM13 Carbohydrate-Binding Module Family 13 GT27 Glycosyltransferase Family 27 |
PTM databases
PhosphoSitePlusi | Q6P9S7 |
SwissPalmi | Q6P9S7 |
Proteomic databases
MaxQBi | Q6P9S7 |
PaxDbi | Q6P9S7 |
PRIDEi | Q6P9S7 |
Genome annotation databases
Ensembli | ENSMUST00000066987; ENSMUSP00000065096; ENSMUSG00000020520 |
GeneIDi | 171212 |
KEGGi | mmu:171212 |
UCSCi | uc007jab.1 mouse |
Organism-specific databases
CTDi | 55568 |
MGIi | MGI:1890480 Galnt10 |
Phylogenomic databases
eggNOGi | KOG3736 Eukaryota ENOG410XPMK LUCA |
GeneTreei | ENSGT00940000156690 |
HOGENOMi | HOG000038227 |
InParanoidi | Q6P9S7 |
KOi | K00710 |
OMAi | MRSVHSL |
OrthoDBi | 276134at2759 |
PhylomeDBi | Q6P9S7 |
TreeFami | TF313267 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Reactomei | R-MMU-913709 O-linked glycosylation of mucins |
Miscellaneous databases
ChiTaRSi | Galnt10 mouse |
PROi | PR:Q6P9S7 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020520 Expressed in 256 organ(s), highest expression level in entire extraembryonic component |
ExpressionAtlasi | Q6P9S7 baseline and differential |
Genevisiblei | Q6P9S7 MM |
Family and domain databases
CDDi | cd00161 RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR001173 Glyco_trans_2-like IPR029044 Nucleotide-diphossugar_trans IPR035992 Ricin_B-like_lectins IPR000772 Ricin_B_lectin |
Pfami | View protein in Pfam PF00535 Glycos_transf_2, 1 hit PF00652 Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458 RICIN, 1 hit |
SUPFAMi | SSF50370 SSF50370, 1 hit SSF53448 SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231 RICIN_B_LECTIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLT10_MOUSE | |
Accessioni | Q6P9S7Primary (citable) accession number: Q6P9S7 Secondary accession number(s): Q6KAQ2, Q8BZU8, Q91YJ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | July 5, 2004 | |
Last modified: | October 16, 2019 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways