UniProtKB - Q6P9F7 (LRC8B_HUMAN)
Volume-regulated anion channel subunit LRRC8B
LRRC8B
Functioni
Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:24790029, PubMed:26824658, PubMed:28193731).
The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:24790029, PubMed:26824658, PubMed:28193731).
3 PublicationsCatalytic activityi
GO - Biological processi
- anion transmembrane transport Source: UniProtKB
- inorganic anion transport Source: GOC
Keywordsi
Molecular function | Ion channel |
Biological process | Ion transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q6P9F7 |
Reactomei | R-HSA-5223345, Miscellaneous transport and binding events |
SignaLinki | Q6P9F7 |
Protein family/group databases
TCDBi | 1.A.25.3.1, the gap junction-forming innexin (innexin) family |
Names & Taxonomyi
Protein namesi | Recommended name: Volume-regulated anion channel subunit LRRC8BCuratedAlternative name(s): Leucine-rich repeat-containing protein 8B1 Publication T-cell activation leucine repeat-rich protein1 Publication Short name: TA-LRRP1 Publication |
Gene namesi | ORF Names:UNQ6413/PRO212071 Publication |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:30692, LRRC8B |
MIMi | 612888, gene |
neXtProti | NX_Q6P9F7 |
VEuPathDBi | HostDB:ENSG00000197147 |
Subcellular locationi
Plasma membrane
- Cell membrane 3 Publications; Multi-pass membrane protein Curated
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication
Note: In the absence of LRRC8A, resides primarily in a cytoplasmic compartment, probably the endoplasmic reticulum. Requires LRRC8A for expression at the cell membrane.1 Publication
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- ion channel complex Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 25 | CytoplasmicSequence analysisAdd BLAST | 25 | |
Transmembranei | 26 – 46 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 47 – 119 | ExtracellularSequence analysisAdd BLAST | 73 | |
Transmembranei | 120 – 140 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 141 – 261 | CytoplasmicSequence analysisAdd BLAST | 121 | |
Transmembranei | 262 – 282 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 283 – 307 | ExtracellularSequence analysisAdd BLAST | 25 | |
Transmembranei | 308 – 328 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 329 – 803 | CytoplasmicSequence analysisAdd BLAST | 475 |
Keywords - Cellular componenti
Cell membrane, Endoplasmic reticulum, MembranePathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000197147 |
PharmGKBi | PA142671535 |
Miscellaneous databases
Pharosi | Q6P9F7, Tbio |
Genetic variation databases
BioMutai | LRRC8B |
DMDMi | 88911355 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076245 | 1 – 803 | Volume-regulated anion channel subunit LRRC8BAdd BLAST | 803 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 55 ↔ 304 | By similarity | ||
Glycosylationi | 78 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 109 ↔ 289 | By similarity | ||
Modified residuei | 186 | PhosphoserineBy similarity | 1 | |
Modified residuei | 196 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q6P9F7 |
jPOSTi | Q6P9F7 |
MassIVEi | Q6P9F7 |
MaxQBi | Q6P9F7 |
PaxDbi | Q6P9F7 |
PeptideAtlasi | Q6P9F7 |
PRIDEi | Q6P9F7 |
ProteomicsDBi | 67042 |
PTM databases
GlyGeni | Q6P9F7, 2 sites |
iPTMneti | Q6P9F7 |
PhosphoSitePlusi | Q6P9F7 |
Expressioni
Gene expression databases
Bgeei | ENSG00000197147, Expressed in primary visual cortex and 217 other tissues |
ExpressionAtlasi | Q6P9F7, baseline and differential |
Genevisiblei | Q6P9F7, HS |
Organism-specific databases
HPAi | ENSG00000197147, Low tissue specificity |
Interactioni
Subunit structurei
Heterohexamer (Probable). Oligomerizes with other LRRC8 proteins (LRRC8A, LRRC8C, LRRC8D and/or LRRC8E) to form a heterohexamer (PubMed:24790029, PubMed:26824658, PubMed:28193731). In vivo, the subunit composition may depend primarily on expression levels, and heterooligomeric channels containing various proportions of the different LRRC8 proteins may coexist (Probable).
Curated3 PublicationsBinary interactionsi
Q6P9F7
With | #Exp. | IntAct |
---|---|---|
LRRC8A [Q8IWT6] | 3 | EBI-9477617,EBI-10970086 |
Protein-protein interaction databases
BioGRIDi | 117054, 32 interactors |
CORUMi | Q6P9F7 |
DIPi | DIP-61361N |
IntActi | Q6P9F7, 21 interactors |
STRINGi | 9606.ENSP00000332674 |
Miscellaneous databases
RNActi | Q6P9F7, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 464 – 486 | LRR 1Add BLAST | 23 | |
Repeati | 488 – 509 | LRR 2Add BLAST | 22 | |
Repeati | 511 – 532 | LRR 3Add BLAST | 22 | |
Repeati | 539 – 559 | LRR 4Add BLAST | 21 | |
Repeati | 562 – 582 | LRR 5Add BLAST | 21 | |
Repeati | 586 – 607 | LRR 6Add BLAST | 22 | |
Repeati | 609 – 630 | LRR 7Add BLAST | 22 | |
Repeati | 634 – 655 | LRR 8Add BLAST | 22 | |
Repeati | 657 – 678 | LRR 9Add BLAST | 22 | |
Repeati | 680 – 701 | LRR 10Add BLAST | 22 | |
Repeati | 703 – 724 | LRR 11Add BLAST | 22 | |
Repeati | 726 – 747 | LRR 12Add BLAST | 22 | |
Repeati | 749 – 771 | LRR 13Add BLAST | 23 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Leucine-rich repeat, Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0619, Eukaryota |
GeneTreei | ENSGT00940000160703 |
HOGENOMi | CLU_019019_0_0_1 |
InParanoidi | Q6P9F7 |
OMAi | CSIDVQA |
OrthoDBi | 298712at2759 |
PhylomeDBi | Q6P9F7 |
TreeFami | TF331443 |
Family and domain databases
Gene3Di | 3.80.10.10, 3 hits |
InterProi | View protein in InterPro IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR026906, LRR_5 IPR032675, LRR_dom_sf IPR021040, LRRC8_Pannexin-like |
Pfami | View protein in Pfam PF13306, LRR_5, 1 hit PF13855, LRR_8, 1 hit PF12534, Pannexin_like, 1 hit |
SMARTi | View protein in SMART SM00369, LRR_TYP, 8 hits |
PROSITEi | View protein in PROSITE PS51450, LRR, 9 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MITLTELKCL ADAQSSYHIL KPWWDVFWYY ITLIMLLVAV LAGALQLTQS
60 70 80 90 100
RVLCCLPCKV EFDNHCAVPW DILKASMNTS SNPGTPLPLP LRIQNDLHRQ
110 120 130 140 150
QYSYIDAVCY EKQLHWFAKF FPYLVLLHTL IFAACSNFWL HYPSTSSRLE
160 170 180 190 200
HFVAILHKCF DSPWTTRALS ETVAEQSVRP LKLSKSKILL SSSGCSADID
210 220 230 240 250
SGKQSLPYPQ PGLESAGIES PTSSVLDKKE GEQAKAIFEK VKRFRMHVEQ
260 270 280 290 300
KDIIYRVYLK QIIVKVILFV LIITYVPYFL THITLEIDCS VDVQAFTGYK
310 320 330 340 350
RYQCVYSLAE IFKVLASFYV ILVILYGLTS SYSLWWMLRS SLKQYSFEAL
360 370 380 390 400
REKSNYSDIP DVKNDFAFIL HLADQYDPLY SKRFSIFLSE VSENKLKQIN
410 420 430 440 450
LNNEWTVEKL KSKLVKNAQD KIELHLFMLN GLPDNVFELT EMEVLSLELI
460 470 480 490 500
PEVKLPSAVS QLVNLKELRV YHSSLVVDHP ALAFLEENLK ILRLKFTEMG
510 520 530 540 550
KIPRWVFHLK NLKELYLSGC VLPEQLSTMQ LEGFQDLKNL RTLYLKSSLS
560 570 580 590 600
RIPQVVTDLL PSLQKLSLDN EGSKLVVLNN LKKMVNLKSL ELISCDLERI
610 620 630 640 650
PHSIFSLNNL HELDLRENNL KTVEEIISFQ HLQNLSCLKL WHNNIAYIPA
660 670 680 690 700
QIGALSNLEQ LSLDHNNIEN LPLQLFLCTK LHYLDLSYNH LTFIPEEIQY
710 720 730 740 750
LSNLQYFAVT NNNIEMLPDG LFQCKKLQCL LLGKNSLMNL SPHVGELSNL
760 770 780 790 800
THLELIGNYL ETLPPELEGC QSLKRNCLIV EENLLNTLPL PVTERLQTCL
DKC
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A7I2RK03 | A0A7I2RK03_HUMAN | Volume-regulated anion channel subu... | LRRC8B | 731 | Annotation score: | ||
C9JGJ7 | C9JGJ7_HUMAN | Volume-regulated anion channel subu... | LRRC8B | 103 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 225 | V → G in AAQ88479 (PubMed:12975309).Curated | 1 | |
Sequence conflicti | 626 | Missing in AAQ88479 (PubMed:12975309).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_051126 | 288 | D → N. Corresponds to variant dbSNP:rs17131746EnsemblClinVar. | 1 | |
Natural variantiVAR_025275 | 419 | Q → K1 PublicationCorresponds to variant dbSNP:rs17855025Ensembl. | 1 | |
Natural variantiVAR_051127 | 469 | R → H. Corresponds to variant dbSNP:rs3795832EnsemblClinVar. | 1 | |
Natural variantiVAR_051128 | 689 | N → S. Corresponds to variant dbSNP:rs12747447Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF385436 mRNA Translation: AAM43837.1 D86984 mRNA Translation: BAA13220.2 Different initiation. AY358112 mRNA Translation: AAQ88479.1 CH471097 Genomic DNA Translation: EAW73137.1 CH471097 Genomic DNA Translation: EAW73138.1 BC030607 mRNA Translation: AAH30607.1 BC060782 mRNA Translation: AAH60782.1 |
CCDSi | CCDS724.1 |
RefSeqi | NP_001127948.1, NM_001134476.1 NP_056165.1, NM_015350.2 XP_005270758.1, XM_005270701.4 XP_005270759.1, XM_005270702.4 XP_005270760.1, XM_005270703.4 XP_011539445.1, XM_011541143.2 XP_011539446.1, XM_011541144.2 XP_011539447.1, XM_011541145.2 XP_011539448.1, XM_011541146.2 XP_016856372.1, XM_017000883.1 XP_016856373.1, XM_017000884.1 XP_016856374.1, XM_017000885.1 |
Genome annotation databases
Ensembli | ENST00000330947; ENSP00000332674; ENSG00000197147 ENST00000639264; ENSP00000492151; ENSG00000197147 ENST00000640258; ENSP00000491377; ENSG00000197147 |
GeneIDi | 23507 |
KEGGi | hsa:23507 |
MANE-Selecti | ENST00000330947.7; ENSP00000332674.2; NM_001369817.2; NP_001356746.1 |
UCSCi | uc057ieo.1, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF385436 mRNA Translation: AAM43837.1 D86984 mRNA Translation: BAA13220.2 Different initiation. AY358112 mRNA Translation: AAQ88479.1 CH471097 Genomic DNA Translation: EAW73137.1 CH471097 Genomic DNA Translation: EAW73138.1 BC030607 mRNA Translation: AAH30607.1 BC060782 mRNA Translation: AAH60782.1 |
CCDSi | CCDS724.1 |
RefSeqi | NP_001127948.1, NM_001134476.1 NP_056165.1, NM_015350.2 XP_005270758.1, XM_005270701.4 XP_005270759.1, XM_005270702.4 XP_005270760.1, XM_005270703.4 XP_011539445.1, XM_011541143.2 XP_011539446.1, XM_011541144.2 XP_011539447.1, XM_011541145.2 XP_011539448.1, XM_011541146.2 XP_016856372.1, XM_017000883.1 XP_016856373.1, XM_017000884.1 XP_016856374.1, XM_017000885.1 |
3D structure databases
SMRi | Q6P9F7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 117054, 32 interactors |
CORUMi | Q6P9F7 |
DIPi | DIP-61361N |
IntActi | Q6P9F7, 21 interactors |
STRINGi | 9606.ENSP00000332674 |
Protein family/group databases
TCDBi | 1.A.25.3.1, the gap junction-forming innexin (innexin) family |
PTM databases
GlyGeni | Q6P9F7, 2 sites |
iPTMneti | Q6P9F7 |
PhosphoSitePlusi | Q6P9F7 |
Genetic variation databases
BioMutai | LRRC8B |
DMDMi | 88911355 |
Proteomic databases
EPDi | Q6P9F7 |
jPOSTi | Q6P9F7 |
MassIVEi | Q6P9F7 |
MaxQBi | Q6P9F7 |
PaxDbi | Q6P9F7 |
PeptideAtlasi | Q6P9F7 |
PRIDEi | Q6P9F7 |
ProteomicsDBi | 67042 |
Protocols and materials databases
Antibodypediai | 19852, 123 antibodies from 15 providers |
DNASUi | 23507 |
Genome annotation databases
Ensembli | ENST00000330947; ENSP00000332674; ENSG00000197147 ENST00000639264; ENSP00000492151; ENSG00000197147 ENST00000640258; ENSP00000491377; ENSG00000197147 |
GeneIDi | 23507 |
KEGGi | hsa:23507 |
MANE-Selecti | ENST00000330947.7; ENSP00000332674.2; NM_001369817.2; NP_001356746.1 |
UCSCi | uc057ieo.1, human |
Organism-specific databases
CTDi | 23507 |
GeneCardsi | LRRC8B |
HGNCi | HGNC:30692, LRRC8B |
HPAi | ENSG00000197147, Low tissue specificity |
MIMi | 612888, gene |
neXtProti | NX_Q6P9F7 |
OpenTargetsi | ENSG00000197147 |
PharmGKBi | PA142671535 |
VEuPathDBi | HostDB:ENSG00000197147 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0619, Eukaryota |
GeneTreei | ENSGT00940000160703 |
HOGENOMi | CLU_019019_0_0_1 |
InParanoidi | Q6P9F7 |
OMAi | CSIDVQA |
OrthoDBi | 298712at2759 |
PhylomeDBi | Q6P9F7 |
TreeFami | TF331443 |
Enzyme and pathway databases
PathwayCommonsi | Q6P9F7 |
Reactomei | R-HSA-5223345, Miscellaneous transport and binding events |
SignaLinki | Q6P9F7 |
Miscellaneous databases
BioGRID-ORCSi | 23507, 6 hits in 1042 CRISPR screens |
ChiTaRSi | LRRC8B, human |
GenomeRNAii | 23507 |
Pharosi | Q6P9F7, Tbio |
PROi | PR:Q6P9F7 |
RNActi | Q6P9F7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000197147, Expressed in primary visual cortex and 217 other tissues |
ExpressionAtlasi | Q6P9F7, baseline and differential |
Genevisiblei | Q6P9F7, HS |
Family and domain databases
Gene3Di | 3.80.10.10, 3 hits |
InterProi | View protein in InterPro IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR026906, LRR_5 IPR032675, LRR_dom_sf IPR021040, LRRC8_Pannexin-like |
Pfami | View protein in Pfam PF13306, LRR_5, 1 hit PF13855, LRR_8, 1 hit PF12534, Pannexin_like, 1 hit |
SMARTi | View protein in SMART SM00369, LRR_TYP, 8 hits |
PROSITEi | View protein in PROSITE PS51450, LRR, 9 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | LRC8B_HUMAN | |
Accessioni | Q6P9F7Primary (citable) accession number: Q6P9F7 Secondary accession number(s): D3DT28 Q92627 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 7, 2006 |
Last sequence update: | February 7, 2006 | |
Last modified: | February 23, 2022 | |
This is version 157 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families