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Protein

Volume-regulated anion channel subunit LRRC8B

Gene

LRRC8B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and may also conduct organic osmolytes like taurine. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition.2 Publications

GO - Molecular functioni

GO - Biological processi

  • anion transmembrane transport Source: UniProtKB
  • cell volume homeostasis Source: InterPro
  • signal transduction Source: GO_Central
  • transmembrane transport Source: Reactome

Keywordsi

Molecular functionIon channel
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-5223345 Miscellaneous transport and binding events

Protein family/group databases

TCDBi1.A.25.3.1 the gap junction-forming innexin (innexin) family

Names & Taxonomyi

Protein namesi
Recommended name:
Volume-regulated anion channel subunit LRRC8B
Alternative name(s):
Leucine-rich repeat-containing protein 8B
T-cell activation leucine repeat-rich protein
Short name:
TA-LRRP
Gene namesi
Name:LRRC8B
Synonyms:KIAA0231
ORF Names:UNQ6413/PRO21207
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000197147.12
HGNCiHGNC:30692 LRRC8B
MIMi612888 gene
neXtProtiNX_Q6P9F7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 25CytoplasmicSequence analysisAdd BLAST25
Transmembranei26 – 46HelicalSequence analysisAdd BLAST21
Topological domaini47 – 119ExtracellularSequence analysisAdd BLAST73
Transmembranei120 – 140HelicalSequence analysisAdd BLAST21
Topological domaini141 – 261CytoplasmicSequence analysisAdd BLAST121
Transmembranei262 – 282HelicalSequence analysisAdd BLAST21
Topological domaini283 – 307ExtracellularSequence analysisAdd BLAST25
Transmembranei308 – 328HelicalSequence analysisAdd BLAST21
Topological domaini329 – 803CytoplasmicSequence analysisAdd BLAST475

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi23507
OpenTargetsiENSG00000197147
PharmGKBiPA142671535

Polymorphism and mutation databases

BioMutaiLRRC8B
DMDMi88911355

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000762451 – 803Volume-regulated anion channel subunit LRRC8BAdd BLAST803

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi78N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei186PhosphoserineBy similarity1
Modified residuei196PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ6P9F7
MaxQBiQ6P9F7
PaxDbiQ6P9F7
PeptideAtlasiQ6P9F7
PRIDEiQ6P9F7
ProteomicsDBi67042

PTM databases

iPTMnetiQ6P9F7
PhosphoSitePlusiQ6P9F7

Expressioni

Gene expression databases

BgeeiENSG00000197147
CleanExiHS_LRRC8B
ExpressionAtlasiQ6P9F7 baseline and differential
GenevisibleiQ6P9F7 HS

Organism-specific databases

HPAiHPA017950

Interactioni

Subunit structurei

Heterooligomer; heterooligomerizes with other LRRC8 proteins (LRRC8A, LRRC8C, LRRC8D and/or LRRC8E), possibly to form a heterohexamer (PubMed:24790029, PubMed:26824658). In vivo, the subunit composition may depend primarily on expression levels, and heterooligomeric channels containing various proportions of the different LRRC8 proteins may coexist (Probable).Curated2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LRRC8AQ8IWT62EBI-9477617,EBI-10970086

Protein-protein interaction databases

BioGridi117054, 22 interactors
CORUMiQ6P9F7
DIPiDIP-61361N
IntActiQ6P9F7, 2 interactors
STRINGi9606.ENSP00000332674

Structurei

3D structure databases

ProteinModelPortaliQ6P9F7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati464 – 486LRR 1Add BLAST23
Repeati488 – 509LRR 2Add BLAST22
Repeati511 – 532LRR 3Add BLAST22
Repeati539 – 559LRR 4Add BLAST21
Repeati562 – 582LRR 5Add BLAST21
Repeati586 – 607LRR 6Add BLAST22
Repeati609 – 630LRR 7Add BLAST22
Repeati634 – 655LRR 8Add BLAST22
Repeati657 – 678LRR 9Add BLAST22
Repeati680 – 701LRR 10Add BLAST22
Repeati703 – 724LRR 11Add BLAST22
Repeati726 – 747LRR 12Add BLAST22
Repeati749 – 771LRR 13Add BLAST23

Sequence similaritiesi

Belongs to the LRRC8 family.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619 Eukaryota
COG4886 LUCA
GeneTreeiENSGT00920000148937
HOGENOMiHOG000231806
HOVERGENiHBG052360
InParanoidiQ6P9F7
KOiK22038
OMAiNSQDKVE
OrthoDBiEOG091G01ER
PhylomeDBiQ6P9F7
TreeFamiTF331443

Family and domain databases

Gene3Di3.80.10.10, 2 hits
InterProiView protein in InterPro
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR026906 LRR_5
IPR032675 LRR_dom_sf
IPR038960 LRRC8
IPR021040 LRRC8_Pannexin-like
PANTHERiPTHR43945 PTHR43945, 1 hit
PfamiView protein in Pfam
PF13306 LRR_5, 1 hit
PF13855 LRR_8, 1 hit
PF12534 Pannexin_like, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 8 hits
PROSITEiView protein in PROSITE
PS51450 LRR, 9 hits

Sequencei

Sequence statusi: Complete.

Q6P9F7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITLTELKCL ADAQSSYHIL KPWWDVFWYY ITLIMLLVAV LAGALQLTQS
60 70 80 90 100
RVLCCLPCKV EFDNHCAVPW DILKASMNTS SNPGTPLPLP LRIQNDLHRQ
110 120 130 140 150
QYSYIDAVCY EKQLHWFAKF FPYLVLLHTL IFAACSNFWL HYPSTSSRLE
160 170 180 190 200
HFVAILHKCF DSPWTTRALS ETVAEQSVRP LKLSKSKILL SSSGCSADID
210 220 230 240 250
SGKQSLPYPQ PGLESAGIES PTSSVLDKKE GEQAKAIFEK VKRFRMHVEQ
260 270 280 290 300
KDIIYRVYLK QIIVKVILFV LIITYVPYFL THITLEIDCS VDVQAFTGYK
310 320 330 340 350
RYQCVYSLAE IFKVLASFYV ILVILYGLTS SYSLWWMLRS SLKQYSFEAL
360 370 380 390 400
REKSNYSDIP DVKNDFAFIL HLADQYDPLY SKRFSIFLSE VSENKLKQIN
410 420 430 440 450
LNNEWTVEKL KSKLVKNAQD KIELHLFMLN GLPDNVFELT EMEVLSLELI
460 470 480 490 500
PEVKLPSAVS QLVNLKELRV YHSSLVVDHP ALAFLEENLK ILRLKFTEMG
510 520 530 540 550
KIPRWVFHLK NLKELYLSGC VLPEQLSTMQ LEGFQDLKNL RTLYLKSSLS
560 570 580 590 600
RIPQVVTDLL PSLQKLSLDN EGSKLVVLNN LKKMVNLKSL ELISCDLERI
610 620 630 640 650
PHSIFSLNNL HELDLRENNL KTVEEIISFQ HLQNLSCLKL WHNNIAYIPA
660 670 680 690 700
QIGALSNLEQ LSLDHNNIEN LPLQLFLCTK LHYLDLSYNH LTFIPEEIQY
710 720 730 740 750
LSNLQYFAVT NNNIEMLPDG LFQCKKLQCL LLGKNSLMNL SPHVGELSNL
760 770 780 790 800
THLELIGNYL ETLPPELEGC QSLKRNCLIV EENLLNTLPL PVTERLQTCL

DKC
Length:803
Mass (Da):92,390
Last modified:February 7, 2006 - v2
Checksum:i9006CE628F427EB9
GO

Sequence cautioni

The sequence BAA13220 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti225V → G in AAQ88479 (PubMed:12975309).Curated1
Sequence conflicti626Missing in AAQ88479 (PubMed:12975309).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051126288D → N. Corresponds to variant dbSNP:rs17131746Ensembl.1
Natural variantiVAR_025275419Q → K1 PublicationCorresponds to variant dbSNP:rs17855025Ensembl.1
Natural variantiVAR_051127469R → H. Corresponds to variant dbSNP:rs3795832Ensembl.1
Natural variantiVAR_051128689N → S. Corresponds to variant dbSNP:rs12747447Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF385436 mRNA Translation: AAM43837.1
D86984 mRNA Translation: BAA13220.2 Different initiation.
AY358112 mRNA Translation: AAQ88479.1
CH471097 Genomic DNA Translation: EAW73137.1
CH471097 Genomic DNA Translation: EAW73138.1
BC030607 mRNA Translation: AAH30607.1
BC060782 mRNA Translation: AAH60782.1
CCDSiCCDS724.1
RefSeqiNP_001127948.1, NM_001134476.1
NP_056165.1, NM_015350.2
XP_005270758.1, XM_005270701.4
XP_005270759.1, XM_005270702.4
XP_005270760.1, XM_005270703.4
XP_011539445.1, XM_011541143.2
XP_011539446.1, XM_011541144.2
XP_011539447.1, XM_011541145.2
XP_011539448.1, XM_011541146.2
XP_016856372.1, XM_017000883.1
XP_016856373.1, XM_017000884.1
XP_016856374.1, XM_017000885.1
UniGeneiHs.482017
Hs.741594

Genome annotation databases

EnsembliENST00000330947; ENSP00000332674; ENSG00000197147
ENST00000439853; ENSP00000400704; ENSG00000197147
ENST00000639264; ENSP00000492151; ENSG00000197147
ENST00000640258; ENSP00000491377; ENSG00000197147
GeneIDi23507
KEGGihsa:23507
UCSCiuc057ieo.1 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiLRC8B_HUMAN
AccessioniPrimary (citable) accession number: Q6P9F7
Secondary accession number(s): D3DT28
, Q6UY21, Q8N106, Q92627
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 7, 2006
Last modified: June 20, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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