Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polypeptide N-acetylgalactosaminyltransferase 18

Gene

GALNT18

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei194SubstrateBy similarity1
Metal bindingi251ManganeseBy similarity1
Metal bindingi253ManganeseBy similarity1
Metal bindingi382ManganeseBy similarity1
Binding sitei385SubstrateBy similarity1
Binding sitei390SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • protein O-linked glycosylation Source: UniProtKB

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandLectin, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-913709 O-linked glycosylation of mucins
UniPathwayiUPA00378

Protein family/group databases

CAZyiCBM13 Carbohydrate-Binding Module Family 13
GT27 Glycosyltransferase Family 27

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 18 (EC:2.4.1.41)
Alternative name(s):
Polypeptide GalNAc transferase 18
Short name:
GalNAc-T18
Polypeptide GalNAc transferase-like protein 4
Short name:
GalNAc-T-like protein 4
Short name:
pp-GaNTase-like protein 4
Polypeptide N-acetylgalactosaminyltransferase-like protein 4
Protein-UDP acetylgalactosaminyltransferase-like protein 4
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 4
Gene namesi
Name:GALNT18
Synonyms:GALNTL4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000110328.5
HGNCiHGNC:30488 GALNT18
MIMi615136 gene
neXtProtiNX_Q6P9A2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 35Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini36 – 607LumenalSequence analysisAdd BLAST572

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi374378
OpenTargetsiENSG00000110328
PharmGKBiPA134950929

Polymorphism and mutation databases

BioMutaiGALNT18
DMDMi116242498

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000591411 – 607Polypeptide N-acetylgalactosaminyltransferase 18Add BLAST607

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi144 ↔ 377PROSITE-ProRule annotation
Glycosylationi146N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi195N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi320N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi368 ↔ 447PROSITE-ProRule annotation
Disulfide bondi482 ↔ 498PROSITE-ProRule annotation
Disulfide bondi530 ↔ 543PROSITE-ProRule annotation
Disulfide bondi571 ↔ 591PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ6P9A2
PaxDbiQ6P9A2
PeptideAtlasiQ6P9A2
PRIDEiQ6P9A2
ProteomicsDBi67031
67032 [Q6P9A2-2]

PTM databases

iPTMnetiQ6P9A2
PhosphoSitePlusiQ6P9A2

Expressioni

Gene expression databases

BgeeiENSG00000110328
CleanExiHS_GALNTL4
ExpressionAtlasiQ6P9A2 baseline and differential
GenevisibleiQ6P9A2 HS

Organism-specific databases

HPAiHPA012955

Interactioni

Protein-protein interaction databases

BioGridi131894, 19 interactors
STRINGi9606.ENSP00000227756

Structurei

3D structure databases

ProteinModelPortaliQ6P9A2
SMRiQ6P9A2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini469 – 599Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST131

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni153 – 267Catalytic subdomain AAdd BLAST115
Regioni324 – 385Catalytic subdomain BAdd BLAST62

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3736 Eukaryota
ENOG410XPMK LUCA
GeneTreeiENSGT00900000140827
HOGENOMiHOG000038228
HOVERGENiHBG051699
InParanoidiQ6P9A2
KOiK00710
OMAiSLFAHWG
OrthoDBiEOG091G036M
PhylomeDBiQ6P9A2
TreeFamiTF313267

Family and domain databases

CDDicd00161 RICIN, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR001173 Glyco_trans_2-like
IPR029044 Nucleotide-diphossugar_trans
IPR035992 Ricin_B-like_lectins
IPR000772 Ricin_B_lectin
PfamiView protein in Pfam
PF00535 Glycos_transf_2, 1 hit
PF00652 Ricin_B_lectin, 1 hit
SMARTiView protein in SMART
SM00458 RICIN, 1 hit
SUPFAMiSSF50370 SSF50370, 1 hit
SSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS50231 RICIN_B_LECTIN, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P9A2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVCTRKTKTL VSTCVILSGM TNIICLLYVG WVTNYIASVY VRGQEPAPDK
60 70 80 90 100
KLEEDKGDTL KIIERLDHLE NVIKQHIQEA PAKPEEAEAE PFTDSSLFAH
110 120 130 140 150
WGQELSPEGR RVALKQFQYY GYNAYLSDRL PLDRPLPDLR PSGCRNLSFP
160 170 180 190 200
DSLPEVSIVF IFVNEALSVL LRSIHSAMER TPPHLLKEII LVDDNSSNEE
210 220 230 240 250
LKEKLTEYVD KVNSQKPGFI KVVRHSKQEG LIRSRVSGWR AATAPVVALF
260 270 280 290 300
DAHVEFNVGW AEPVLTRIKE NRKRIISPSF DNIKYDNFEI EEYPLAAQGF
310 320 330 340 350
DWELWCRYLN PPKAWWKLEN STAPIRSPAL IGCFIVDRQY FQEIGLLDEG
360 370 380 390 400
MEVYGGENVE LGIRVWQCGG SVEVLPCSRI AHIERAHKPY TEDLTAHVRR
410 420 430 440 450
NALRVAEVWM DEFKSHVYMA WNIPQEDSGI DIGDITARKA LRKQLQCKTF
460 470 480 490 500
RWYLVSVYPE MRMYSDIIAY GVLQNSLKTD LCLDQGPDTE NVPIMYICHG
510 520 530 540 550
MTPQNVYYTS SQQIHVGILS PTVDDDDNRC LVDVNSRPRL IECSYAKAKR
560 570 580 590 600
MKLHWQFSQG GPIQNRKSKR CLELQENSDL EFGFQLVLQK CSGQHWSITN

VLRSLAS
Length:607
Mass (Da):69,561
Last modified:October 17, 2006 - v2
Checksum:i8FFA7BCB5016FF0C
GO
Isoform 2 (identifier: Q6P9A2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     79-91: EAPAKPEEAEAEP → GYRRNFSLLNVSN
     92-607: Missing.

Note: No experimental confirmation available.
Show »
Length:91
Mass (Da):10,397
Checksum:i4EBD1A8E212F25C7
GO

Sequence cautioni

The sequence AAD20062 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti133D → G in AAH60864 (PubMed:15489334).Curated1
Sequence conflicti541I → T in AAH60864 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01123479 – 91EAPAK…AEAEP → GYRRNFSLLNVSN in isoform 2. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_01123592 – 607Missing in isoform 2. 1 PublicationAdd BLAST516

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF131852 mRNA Translation: AAD20062.1 Different initiation.
BC060864 mRNA Translation: AAH60864.1
BC037341 mRNA Translation: AAH37341.3
CCDSiCCDS7807.1 [Q6P9A2-1]
RefSeqiNP_940918.2, NM_198516.2 [Q6P9A2-1]
UniGeneiHs.655152
Hs.667788

Genome annotation databases

EnsembliENST00000227756; ENSP00000227756; ENSG00000110328 [Q6P9A2-1]
GeneIDi374378
KEGGihsa:374378
UCSCiuc001mjo.3 human [Q6P9A2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGLT18_HUMAN
AccessioniPrimary (citable) accession number: Q6P9A2
Secondary accession number(s): O95903, Q8NDY9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: October 17, 2006
Last modified: July 18, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health