UniProtKB - Q6P7W0 (SENP6_MOUSE)
Sentrin-specific protease 6
Senp6
Functioni
Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly-SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination.
: protein sumoylation Pathwayi
This protein is involved in the pathway protein sumoylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 785 | By similarity | 1 | |
Active sitei | 936 | By similarity | 1 | |
Active sitei | 1049 | By similarity | 1 |
GO - Molecular functioni
- SUMO-specific endopeptidase activity Source: UniProtKB
GO - Biological processi
- protein desumoylation Source: UniProtKB
- protein modification by small protein removal Source: UniProtKB
- protein sumoylation Source: UniProtKB-UniPathway
- regulation of kinetochore assembly Source: UniProtKB
- regulation of spindle assembly Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
BRENDAi | 3.4.22.B74, 3474 |
UniPathwayi | UPA00886 |
Protein family/group databases
MEROPSi | C48.004 |
Names & Taxonomyi
Protein namesi | Recommended name: Sentrin-specific protease 6 (EC:3.4.22.-)Alternative name(s): SUMO-1-specific protease 1 Sentrin/SUMO-specific protease SENP6 |
Gene namesi | Name:Senp6 Synonyms:Kiaa0797, Susp1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1922075, Senp6 |
VEuPathDBi | HostDB:ENSMUSG00000034252 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000267608 | 1 – 1132 | Sentrin-specific protease 6Add BLAST | 1132 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 41 | PhosphoserineBy similarity | 1 | |
Modified residuei | 355 | PhosphoserineCombined sources | 1 | |
Modified residuei | 356 | PhosphoserineCombined sources | 1 | |
Modified residuei | 371 | PhosphoserineBy similarity | 1 | |
Modified residuei | 373 | PhosphoserineBy similarity | 1 | |
Modified residuei | 436 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 648 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 938 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1131 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q6P7W0 |
MaxQBi | Q6P7W0 |
PaxDbi | Q6P7W0 |
PeptideAtlasi | Q6P7W0 |
PRIDEi | Q6P7W0 |
ProteomicsDBi | 256545 [Q6P7W0-1] 256546 [Q6P7W0-2] |
PTM databases
iPTMneti | Q6P7W0 |
PhosphoSitePlusi | Q6P7W0 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000034252, Expressed in primary oocyte and 307 other tissues |
ExpressionAtlasi | Q6P7W0, baseline and differential |
Genevisiblei | Q6P7W0, MM |
Interactioni
Subunit structurei
Interacts with RXRA.
Forms a complex with KAT5-TIP60 and UBE2I in response to UV irradiation.
Interacts with RPA1 to maintain it in hyposumoylated state during S phase preventing DNA repair initiation (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 229616, 6 interactors |
IntActi | Q6P7W0, 2 interactors |
MINTi | Q6P7W0 |
STRINGi | 10090.ENSMUSP00000047220 |
Miscellaneous databases
RNActi | Q6P7W0, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 23 – 51 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 327 – 388 | DisorderedSequence analysisAdd BLAST | 62 | |
Regioni | 686 – 1132 | ProteaseAdd BLAST | 447 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0779, Eukaryota |
GeneTreei | ENSGT00940000155724 |
InParanoidi | Q6P7W0 |
OMAi | DENYTSE |
OrthoDBi | 1241636at2759 |
TreeFami | TF350136 |
Family and domain databases
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR003653, Peptidase_C48_C |
Pfami | View protein in Pfam PF02902, Peptidase_C48, 2 hits |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50600, ULP_PROTEASE, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAGKSGGSA GALFLKALDR SESKRDGGFK NNWSFDHEEE SEGDADKDGA
60 70 80 90 100
NLLSVEDEDS EISKGKKLNR RSEIVATSSG DFILKTYVRR SKTDGFKTLK
110 120 130 140 150
GNPIGLNMLS NNKKLSESTA GTALCSGTVV HGRRFHHAHS QTPGIRTAAQ
160 170 180 190 200
RKEYPPYVHK AENSPVMLSH GQGGDHIMKK TEESESYVES EIKRKVQQKR
210 220 230 240 250
HCSTYQLSPL SPASKKCLTH LEVSEQREYC PKCGKEKENQ TKCQSCGIVF
260 270 280 290 300
HNDLQRNCRQ AVTLNEPTGP LLRTSIHQNS GGQKSQNTGL TAKKFYGNSV
310 320 330 340 350
DKIPIDILVT CDDSRHNYIQ TNGKVILPGG KIPKLTNPKE RKISVSDLND
360 370 380 390 400
PIILSSDDDD DDDDRTKRRE STSPKPADSA CSSPVPSTGK VEAALNADAC
410 420 430 440 450
RAEQEPRSSP AEPELNTIVI PRKARMKDQL GNSISTPLKR RKVNSHAAFI
460 470 480 490 500
HPMSLSCQNF ESVILNCRSI RVGTLFRLLV EPVIFSLESI TIHLDGPESD
510 520 530 540 550
PVDIILNTSD LTKCEWCNVR KLPVVFLQAI PAVYQKLSMQ LQMSKEDKVW
560 570 580 590 600
NDCKGINRIT SLEEQYIILI FQTGLDHQAE VVFESIITDI GIRNNVPNFF
610 620 630 640 650
AKILFDEANS RLVACTRSYE ESIKGNCAQK ENKVKTVSFE SKIQLRSKQE
660 670 680 690 700
LQFFDDDEEA GESHTIFIGP VEKLIVYPPP PAKGGISVTN EDLHCLSEGE
710 720 730 740 750
FLNDVIIDFY LKYLVLEKLK KEDADRIHIF SSFFYKRLNQ RERRNPETTN
760 770 780 790 800
LSIQQKRHGR VKTWTRHVDI FEKDFIFVPL NEAAHWFLAV VCFPGLEKPK
810 820 830 840 850
YEPNPHYHEN AVMQKTPSAE DSCVSSASEM GACSQNSAAK PVIKKMLNRK
860 870 880 890 900
HCLAVTDSSA AQEESEPCYR RNAYSVKCSM KKKNHAINEN EEPSNGESTC
910 920 930 940 950
QDICDRTQSE NGLRDECFSS VHHPDALSKI RLNYGDQSAD GGKLLEDELI
960 970 980 990 1000
DFSEDQDDPD DSSDDGLLAD ENYSSEIGQW HLKPTVCKQP CILLMDSLRG
1010 1020 1030 1040 1050
PSRSNVVKIL REYLEVEWEV KKGSKRSFSK DVMKGSNPKV PQQNNFSDCG
1060 1070 1080 1090 1100
VYVLQYVESF FENPVLNFEL PMNLMNWFPP PRMKTKREEI RNIILKLQES
1110 1120 1130
QSKDKKLLKD SLAETSLGDG AEQYASASGG SE
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6Z9A1 | F6Z9A1_MOUSE | Sentrin-specific protease 6 | Senp6 | 1,139 | Annotation score: | ||
H3BLA9 | H3BLA9_MOUSE | Sentrin-specific protease 6 | Senp6 | 204 | Annotation score: | ||
H3BJS8 | H3BJS8_MOUSE | Sentrin-specific protease 6 | Senp6 | 285 | Annotation score: | ||
H3BKR4 | H3BKR4_MOUSE | Sentrin-specific protease 6 | Senp6 | 131 | Annotation score: | ||
H3BK20 | H3BK20_MOUSE | Sentrin-specific protease 6 | Senp6 | 179 | Annotation score: | ||
H3BKI2 | H3BKI2_MOUSE | Sentrin-specific protease 6 | Senp6 | 48 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 121 | G → A in AAH61480 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 375 | K → Q in BAC98029 (PubMed:14621295).Curated | 1 | |
Sequence conflicti | 375 | K → Q in AAH61480 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 747 | E → D in BAC98029 (PubMed:14621295).Curated | 1 | |
Sequence conflicti | 747 | E → D in AAH61480 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1075 | M → V in BAC98029 (PubMed:14621295).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_021943 | 1 – 166 | Missing in isoform 2. 1 PublicationAdd BLAST | 166 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK129219 mRNA Translation: BAC98029.1 Different initiation. AC142111 Genomic DNA No translation available. BC061480 mRNA Translation: AAH61480.1 Frameshift. AK053904 mRNA Translation: BAC35583.1 Sequence problems. AK083377 mRNA Translation: BAC38892.2 |
CCDSi | CCDS40705.1 [Q6P7W0-1] |
RefSeqi | NP_001298039.1, NM_001311110.1 NP_666115.2, NM_146003.2 [Q6P7W0-1] |
Genome annotation databases
Ensembli | ENSMUST00000037484; ENSMUSP00000047220; ENSMUSG00000034252 [Q6P7W0-1] ENSMUST00000164859; ENSMUSP00000128918; ENSMUSG00000034252 [Q6P7W0-2] |
GeneIDi | 215351 |
KEGGi | mmu:215351 |
UCSCi | uc009qvb.1, mouse [Q6P7W0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK129219 mRNA Translation: BAC98029.1 Different initiation. AC142111 Genomic DNA No translation available. BC061480 mRNA Translation: AAH61480.1 Frameshift. AK053904 mRNA Translation: BAC35583.1 Sequence problems. AK083377 mRNA Translation: BAC38892.2 |
CCDSi | CCDS40705.1 [Q6P7W0-1] |
RefSeqi | NP_001298039.1, NM_001311110.1 NP_666115.2, NM_146003.2 [Q6P7W0-1] |
3D structure databases
SMRi | Q6P7W0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 229616, 6 interactors |
IntActi | Q6P7W0, 2 interactors |
MINTi | Q6P7W0 |
STRINGi | 10090.ENSMUSP00000047220 |
Protein family/group databases
MEROPSi | C48.004 |
PTM databases
iPTMneti | Q6P7W0 |
PhosphoSitePlusi | Q6P7W0 |
Proteomic databases
jPOSTi | Q6P7W0 |
MaxQBi | Q6P7W0 |
PaxDbi | Q6P7W0 |
PeptideAtlasi | Q6P7W0 |
PRIDEi | Q6P7W0 |
ProteomicsDBi | 256545 [Q6P7W0-1] 256546 [Q6P7W0-2] |
Protocols and materials databases
Antibodypediai | 7742, 421 antibodies from 32 providers |
DNASUi | 215351 |
Genome annotation databases
Ensembli | ENSMUST00000037484; ENSMUSP00000047220; ENSMUSG00000034252 [Q6P7W0-1] ENSMUST00000164859; ENSMUSP00000128918; ENSMUSG00000034252 [Q6P7W0-2] |
GeneIDi | 215351 |
KEGGi | mmu:215351 |
UCSCi | uc009qvb.1, mouse [Q6P7W0-1] |
Organism-specific databases
CTDi | 26054 |
MGIi | MGI:1922075, Senp6 |
VEuPathDBi | HostDB:ENSMUSG00000034252 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG0779, Eukaryota |
GeneTreei | ENSGT00940000155724 |
InParanoidi | Q6P7W0 |
OMAi | DENYTSE |
OrthoDBi | 1241636at2759 |
TreeFami | TF350136 |
Enzyme and pathway databases
UniPathwayi | UPA00886 |
BRENDAi | 3.4.22.B74, 3474 |
Miscellaneous databases
BioGRID-ORCSi | 215351, 9 hits in 64 CRISPR screens |
ChiTaRSi | Senp6, mouse |
PROi | PR:Q6P7W0 |
RNActi | Q6P7W0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000034252, Expressed in primary oocyte and 307 other tissues |
ExpressionAtlasi | Q6P7W0, baseline and differential |
Genevisiblei | Q6P7W0, MM |
Family and domain databases
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR003653, Peptidase_C48_C |
Pfami | View protein in Pfam PF02902, Peptidase_C48, 2 hits |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50600, ULP_PROTEASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SENP6_MOUSE | |
Accessioni | Q6P7W0Primary (citable) accession number: Q6P7W0 Secondary accession number(s): E9QN09 Q8BUL4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 12, 2006 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 119 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families