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UniProtKB - Q6P7A9 (LYAG_RAT)
Protein
Lysosomal alpha-glucosidase
Gene
Gaa
Organism
Rattus norvegicus (Rat)
Status
Functioni
Essential for the degradation of glycogen in lysosomes. Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans.
By similarityCatalytic activityi
- Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.By similarity EC:3.2.1.20
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 404 | SubstrateBy similarity | 1 | |
Active sitei | 518 | NucleophilePROSITE-ProRule annotation | 1 | |
Active sitei | 521 | By similarity | 1 | |
Binding sitei | 600 | SubstrateBy similarity | 1 | |
Binding sitei | 616 | SubstrateBy similarity | 1 | |
Binding sitei | 674 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- alpha-1,4-glucosidase activity Source: RGD
- alpha-glucosidase activity Source: RGD
- carbohydrate binding Source: RGD
- hydrolase activity, hydrolyzing O-glycosyl compounds Source: GO_Central
- maltose alpha-glucosidase activity Source: UniProtKB-EC
GO - Biological processi
- cardiac muscle contraction Source: RGD
- diaphragm contraction Source: RGD
- glycogen catabolic process Source: RGD
- glycogen metabolic process Source: RGD
- heart morphogenesis Source: RGD
- locomotory behavior Source: RGD
- lysosome organization Source: RGD
- muscle cell cellular homeostasis Source: RGD
- neuromuscular process controlling balance Source: RGD
- neuromuscular process controlling posture Source: RGD
- regulation of the force of heart contraction Source: RGD
- striated muscle contraction Source: RGD
- tissue development Source: RGD
- vacuolar sequestering Source: RGD
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Enzyme and pathway databases
Reactomei | R-RNO-6798695, Neutrophil degranulation R-RNO-70221, Glycogen breakdown (glycogenolysis) |
SABIO-RKi | Q6P7A9 |
Protein family/group databases
CAZyi | GH31, Glycoside Hydrolase Family 31 |
Names & Taxonomyi
Protein namesi | Recommended name: Lysosomal alpha-glucosidase (EC:3.2.1.20By similarity)Alternative name(s): Acid maltase |
Gene namesi | Name:Gaa |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 735227, Gaa |
Subcellular locationi
Lysosome
- Lysosome By similarity
- Lysosome membrane By similarity
Lysosome
- lysosomal membrane Source: UniProtKB-SubCell
- lysosome Source: RGD
Other locations
- membrane Source: RGD
Keywords - Cellular componenti
Lysosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | Sequence analysisAdd BLAST | 27 | |
PropeptideiPRO_0000260442 | 28 – 69 | By similarityAdd BLAST | 42 | |
ChainiPRO_0000260443 | 70 – 953 | Lysosomal alpha-glucosidaseAdd BLAST | 884 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 82 ↔ 109 | PROSITE-ProRule annotation | ||
Disulfide bondi | 92 ↔ 108 | PROSITE-ProRule annotation | ||
Disulfide bondi | 103 ↔ 127 | PROSITE-ProRule annotation | ||
Glycosylationi | 140 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 233 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 390 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 470 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 533 ↔ 558 | By similarity | ||
Disulfide bondi | 647 ↔ 658 | By similarity | ||
Glycosylationi | 652 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 883 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 926 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
jPOSTi | Q6P7A9 |
PaxDbi | Q6P7A9 |
PRIDEi | Q6P7A9 |
PTM databases
GlyGeni | Q6P7A9, 7 sites, 2 N-linked glycans (1 site) |
iPTMneti | Q6P7A9 |
PhosphoSitePlusi | Q6P7A9 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 266559, 1 interactor |
IntActi | Q6P7A9, 1 interactor |
STRINGi | 10116.ENSRNOP00000064500 |
Chemistry databases
BindingDBi | Q6P7A9 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 80 – 131 | P-typePROSITE-ProRule annotationAdd BLAST | 52 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 31 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1065, Eukaryota |
InParanoidi | Q6P7A9 |
OrthoDBi | 151244at2759 |
PhylomeDBi | Q6P7A9 |
Family and domain databases
CDDi | cd00111, Trefoil, 1 hit |
Gene3Di | 2.60.40.1180, 2 hits 4.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR031727, Gal_mutarotase_N IPR011013, Gal_mutarotase_sf_dom IPR000322, Glyco_hydro_31 IPR030458, Glyco_hydro_31_AS IPR030459, Glyco_hydro_31_CS IPR025887, Glyco_hydro_31_N_dom IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF IPR017957, P_trefoil_CS IPR000519, P_trefoil_dom IPR044913, P_trefoil_dom_sf |
Pfami | View protein in Pfam PF13802, Gal_mutarotas_2, 1 hit PF01055, Glyco_hydro_31, 1 hit PF16863, NtCtMGAM_N, 1 hit PF00088, Trefoil, 1 hit |
SMARTi | View protein in SMART SM00018, PD, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit SSF74650, SSF74650, 1 hit |
PROSITEi | View protein in PROSITE PS00129, GLYCOSYL_HYDROL_F31_1, 1 hit PS00707, GLYCOSYL_HYDROL_F31_2, 1 hit PS00025, P_TREFOIL_1, 1 hit PS51448, P_TREFOIL_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q6P7A9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNIRKPLCSN SVVGACTLVS LTTAVILGHL MLRELMLLPQ DLHESSSGLW
60 70 80 90 100
KTYRPHHQES YEPAPLHIQE HAEQLRAVPT QCDVTPNSRF DCAPDKGITQ
110 120 130 140 150
EQCEARGCCW VPAGQVLNGP VMGQPWCFFP PSYPSYRLEN LSSTESGYTA
160 170 180 190 200
TLTRTSPTFF PKDVLTLQLE VLMETDSRLH FMIKDPTSKR YEVPLETPRV
210 220 230 240 250
LSQAPSPLYS VEFSEEPFGV IVRRKLGGRV LLNTTVAPLF FADQFLQLST
260 270 280 290 300
SLPSQHIAGL GEHLSPLMLS TEWTRITLWN RDVAPSQGVN LYGSHPFYLA
310 320 330 340 350
LEDGGLAHGV FLLNSNAMDV VLQPSPALTW RSTGGILDVY VFLGPEPKSV
360 370 380 390 400
VQQYLDVVGY PFMPPYWGLG FHLCRWGYSS TAIVRQVVEN MTRTHFPLDV
410 420 430 440 450
QWNDLDYMDA RRDFTFNQDG FADFPDMVHE LHQGGRRYMM IVDPAISSSG
460 470 480 490 500
PAGSYRPYDE GLRRGVFITN ETGQPLIGKV WPGSTAFPDF TNPETLDWWQ
510 520 530 540 550
DMVSEFHAQV PFDGMWIDMN EPSNFIRGSQ QGCPDNELEN PPYVPGVVGG
560 570 580 590 600
ALQAATICAS SHQFLSTHYN LHNLYGLTEA IASSRALVKT RGTRPFVISR
610 620 630 640 650
STFAGHGRYA GHWTGDVWSS WEHLAYSVPE ILQFNLLGVP LVGADICGFQ
660 670 680 690 700
GNTTEELCVR WTQLGAFYPF MRNHNDLNSL PQEPYRFSET AQQAMRKAFT
710 720 730 740 750
LRYALLPYLY TLFHGAHVKG DTVARPLFLE FPEDPSTWSV DRQLLWGPAL
760 770 780 790 800
LITPVLEPGK TDVTGYFPKG MWYNLQMVPV ETLGSLPSSS PASSFRSIVH
810 820 830 840 850
SKGQWLTLEA PLDTINVHLR AGYIIPLQGP SLTTTESRKQ PMALAVALTE
860 870 880 890 900
SGEASGELFW DDGESLGVLE RGAYTLVTFS AKNNTIVNKL VHVTKEGGEL
910 920 930 940 950
QLREVTILGV TTAPTQVLSN GISVSNFTYS PDDKSLSIPV SLLMGERFQI
DWS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM0R544 | M0R544_RAT | Glucosidase, alpha, acid, isoform C... | Gaa rCG_33228 | 953 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC061753 mRNA Translation: AAH61753.1 |
RefSeqi | NP_954549.1, NM_199118.1 |
Genome annotation databases
GeneIDi | 367562 |
KEGGi | rno:367562 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC061753 mRNA Translation: AAH61753.1 |
RefSeqi | NP_954549.1, NM_199118.1 |
3D structure databases
AlphaFoldDBi | Q6P7A9 |
SMRi | Q6P7A9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 266559, 1 interactor |
IntActi | Q6P7A9, 1 interactor |
STRINGi | 10116.ENSRNOP00000064500 |
Chemistry databases
BindingDBi | Q6P7A9 |
ChEMBLi | CHEMBL3513 |
DrugCentrali | Q6P7A9 |
Protein family/group databases
CAZyi | GH31, Glycoside Hydrolase Family 31 |
PTM databases
GlyGeni | Q6P7A9, 7 sites, 2 N-linked glycans (1 site) |
iPTMneti | Q6P7A9 |
PhosphoSitePlusi | Q6P7A9 |
Proteomic databases
jPOSTi | Q6P7A9 |
PaxDbi | Q6P7A9 |
PRIDEi | Q6P7A9 |
Genome annotation databases
GeneIDi | 367562 |
KEGGi | rno:367562 |
Organism-specific databases
CTDi | 2548 |
RGDi | 735227, Gaa |
Phylogenomic databases
eggNOGi | KOG1065, Eukaryota |
InParanoidi | Q6P7A9 |
OrthoDBi | 151244at2759 |
PhylomeDBi | Q6P7A9 |
Enzyme and pathway databases
Reactomei | R-RNO-6798695, Neutrophil degranulation R-RNO-70221, Glycogen breakdown (glycogenolysis) |
SABIO-RKi | Q6P7A9 |
Miscellaneous databases
PROi | PR:Q6P7A9 |
Family and domain databases
CDDi | cd00111, Trefoil, 1 hit |
Gene3Di | 2.60.40.1180, 2 hits 4.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR031727, Gal_mutarotase_N IPR011013, Gal_mutarotase_sf_dom IPR000322, Glyco_hydro_31 IPR030458, Glyco_hydro_31_AS IPR030459, Glyco_hydro_31_CS IPR025887, Glyco_hydro_31_N_dom IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF IPR017957, P_trefoil_CS IPR000519, P_trefoil_dom IPR044913, P_trefoil_dom_sf |
Pfami | View protein in Pfam PF13802, Gal_mutarotas_2, 1 hit PF01055, Glyco_hydro_31, 1 hit PF16863, NtCtMGAM_N, 1 hit PF00088, Trefoil, 1 hit |
SMARTi | View protein in SMART SM00018, PD, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit SSF74650, SSF74650, 1 hit |
PROSITEi | View protein in PROSITE PS00129, GLYCOSYL_HYDROL_F31_1, 1 hit PS00707, GLYCOSYL_HYDROL_F31_2, 1 hit PS00025, P_TREFOIL_1, 1 hit PS51448, P_TREFOIL_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LYAG_RAT | |
Accessioni | Q6P7A9Primary (citable) accession number: Q6P7A9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2006 |
Last sequence update: | July 5, 2004 | |
Last modified: | May 25, 2022 | |
This is version 94 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families