UniProtKB - Q6P730 (DAB2P_RAT)
Disabled homolog 2-interacting protein
Dab2ip
Functioni
GO - Molecular functioni
- 14-3-3 protein binding Source: RGD
- death receptor binding Source: RGD
- GTPase activator activity Source: RGD
- identical protein binding Source: RGD
- kinase binding Source: RGD
- mitogen-activated protein kinase kinase binding Source: RGD
- mitogen-activated protein kinase kinase kinase binding Source: RGD
- phosphatidylinositol 3-kinase binding Source: UniProtKB
- phosphatidylinositol 3-kinase regulatory subunit binding Source: UniProtKB
- phosphatidylinositol-3-phosphate binding Source: UniProtKB
- phosphatidylinositol-4-phosphate binding Source: UniProtKB
- protein-containing complex binding Source: UniProtKB
- protein homodimerization activity Source: RGD
- protein kinase binding Source: RGD
- protein N-terminus binding Source: RGD
- protein phosphatase 2A binding Source: RGD
- SH3 domain binding Source: UniProtKB
- signaling adaptor activity Source: RGD
- vascular endothelial growth factor receptor 2 binding Source: RGD
GO - Biological processi
- activation of JUN kinase activity Source: RGD
- activation of MAPKKK activity Source: UniProtKB
- angiogenesis Source: UniProtKB-KW
- cell cycle Source: UniProtKB-KW
- cell motility involved in cerebral cortex radial glia guided migration Source: UniProtKB
- cellular protein catabolic process Source: UniProtKB
- cellular response to epidermal growth factor stimulus Source: BHF-UCL
- cellular response to interleukin-1 Source: UniProtKB
- cellular response to lipopolysaccharide Source: UniProtKB
- cellular response to tumor necrosis factor Source: UniProtKB
- cellular response to vascular endothelial growth factor stimulus Source: UniProtKB
- extrinsic apoptotic signaling pathway via death domain receptors Source: RGD
- I-kappaB phosphorylation Source: UniProtKB
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: RGD
- layer formation in cerebral cortex Source: UniProtKB
- negative regulation of angiogenesis Source: UniProtKB
- negative regulation of canonical Wnt signaling pathway Source: RGD
- negative regulation of cell growth Source: RGD
- negative regulation of cell population proliferation Source: UniProtKB
- negative regulation of cellular protein catabolic process Source: UniProtKB
- negative regulation of endothelial cell migration Source: UniProtKB
- negative regulation of epidermal growth factor receptor signaling pathway Source: BHF-UCL
- negative regulation of epithelial cell migration Source: UniProtKB
- negative regulation of epithelial cell proliferation Source: UniProtKB
- negative regulation of epithelial to mesenchymal transition Source: UniProtKB
- negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
- negative regulation of fibroblast proliferation Source: UniProtKB
- negative regulation of G0 to G1 transition Source: UniProtKB
- negative regulation of GTPase activity Source: UniProtKB
- negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- negative regulation of MAP kinase activity Source: UniProtKB
- negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
- negative regulation of phosphatidylinositol 3-kinase activity Source: UniProtKB
- negative regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
- negative regulation of protein phosphorylation Source: UniProtKB
- negative regulation of protein serine/threonine kinase activity Source: UniProtKB
- negative regulation of Ras protein signal transduction Source: RGD
- negative regulation of toll-like receptor 4 signaling pathway Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- negative regulation of vascular endothelial growth factor receptor signaling pathway Source: UniProtKB
- negative regulation of vascular endothelial growth factor signaling pathway Source: UniProtKB
- neuron projection morphogenesis Source: UniProtKB
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of apoptotic signaling pathway Source: UniProtKB
- positive regulation of cell cycle arrest Source: UniProtKB
- positive regulation of dendrite development Source: UniProtKB
- positive regulation of epithelial cell proliferation Source: UniProtKB
- positive regulation of JNK cascade Source: UniProtKB
- positive regulation of JUN kinase activity Source: RGD
- positive regulation of MAPK cascade Source: UniProtKB
- positive regulation of neuron migration Source: UniProtKB
- positive regulation of neuron projection development Source: UniProtKB
- positive regulation of proteasomal protein catabolic process Source: UniProtKB
- positive regulation of protein catabolic process Source: UniProtKB
- positive regulation of protein-containing complex assembly Source: RGD
- positive regulation of protein serine/threonine kinase activity Source: UniProtKB
- positive regulation of synapse maturation Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- reelin-mediated signaling pathway Source: InterPro
- regulation of GTPase activity Source: UniProtKB
- regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- regulation of p38MAPK cascade Source: UniProtKB
- regulation of protein-containing complex assembly Source: UniProtKB
- response to unfolded protein Source: UniProtKB-KW
- tube formation Source: UniProtKB
- vascular endothelial growth factor receptor-2 signaling pathway Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, GTPase activation |
Biological process | Angiogenesis, Apoptosis, Cell cycle, Growth regulation, Stress response, Unfolded protein response |
Enzyme and pathway databases
Reactomei | R-RNO-5658442, Regulation of RAS by GAPs |
Names & Taxonomyi
Protein namesi | Recommended name: Disabled homolog 2-interacting proteinShort name: DAB2-interacting protein Alternative name(s): ASK-interacting protein 1 Short name: AIP-1 DIP1/2 DOC-2/DAB2 interactive protein |
Gene namesi | Name:Dab2ip |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621686, Dab2ip |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
Other locations
Note: Localized in soma and dendrites of Purkinje cells as well as in scattered cell bodies in the molecular layer of the cerebellum. Colocalizes with TIRAP at the plasma membrane. Colocalizes with ARF6 at the plasma membrane and endocytic vesicles. Translocates from the plasma membrane to the cytoplasm in response to TNF-alpha. Phosphatidylinositol 4-phosphate (PtdIns4P) binding is essential for plasma membrane localization (By similarity).By similarity
Plasma Membrane
- neuronal cell body membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
Other locations
- AIP1-IRE1 complex Source: RGD
- axon Source: UniProtKB
- cerebellar mossy fiber Source: UniProtKB
- climbing fiber Source: UniProtKB
- cytoplasm Source: UniProtKB
- dendrite Source: UniProtKB-SubCell
- endocytic vesicle Source: UniProtKB
- neuronal cell body Source: UniProtKB
- parallel fiber Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 220 | R → L: Loss of GAP activity. 1 Publication | 1 |
Keywords - Diseasei
Tumor suppressorPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000252409 | 1 – 996 | Disabled homolog 2-interacting proteinAdd BLAST | 996 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 535 | Phosphoserine; by MAP3K5 and RIPK1By similarity | 1 | |
Modified residuei | 554 | PhosphoserineCombined sources | 1 | |
Modified residuei | 785 | PhosphoserineBy similarity | 1 | |
Modified residuei | 802 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q6P730 |
PaxDbi | Q6P730 |
PRIDEi | Q6P730 |
PTM databases
iPTMneti | Q6P730 |
PhosphoSitePlusi | Q6P730 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000055226, Expressed in skeletal muscle tissue and 20 other tissues |
ExpressionAtlasi | Q6P730, baseline and differential |
Genevisiblei | Q6P730, RN |
Interactioni
Subunit structurei
On plasma membrane, exists in an inactive form complexed with TNFR1; in response to TNF-alpha, dissociates from TNFR1 complex, translocates to cytoplasm and forms part of an intracellular signaling complex comprising TRADD, RALBP1, TRAF2 and MAP3K5.
Interacts (via NPXY motif) with DAB2 (via PID domain).
Interacts (via PH domain) with ERN1.
Part of a cytoplasmic complex made of HIPK1, DAB2IP and MAP3K5 in response to TNF-alpha; this complex formation promotes MAP3K5-JNK activation and subsequent apoptosis.
Interacts (via N-terminal domain) with JAK2; the interaction occurs in a IFNG/IFN-gamma-dependent manner and inhibits JAK2 autophosphorylation activity.
Interacts (via C2 domain) with GSK3B; the interaction stimulates GSK3B kinase activation.
Interacts (via C2 domain) with PPP2CA.
Interacts (via proline-rich motif) with a regulatory p85 subunit (via SH3 domain) of the PI3K complex; the interaction inhibits the PI3K-AKT complex activity in a TNF-alpha-dependent manner in prostate cancer (PCa) cells.
Interacts with AKT1; the interaction is increased in a TNF-alpha-induced manner.
Interacts (via C2 domain and active form preferentially) with KDR/VEGFR2 (tyrosine-phosphorylated active form preferentially); the interaction occurs at the late phase of VEGFA response and inhibits KDR/VEGFR2 activity.
Interacts (via N-terminus C2 domain) with MAP3K5 ('Ser-966' dephosphorylated form preferentially); the interaction occurs in a TNF-alpha-induced manner.
Interacts (via Ras-GAP domain) with the catalytic subunit of protein phosphatase PP2A; the interaction occurs in resting endothelial cells, is further enhanced by TNF-alpha stimulation and is required to bridge PP2A to MAP3K5.
Interacts (via C-terminus PER domain) with TRAF2 (via zinc fingers); the interaction occurs in a TNF-alpha-dependent manner.
Interacts with 14-3-3 proteins; the interaction occurs in a TNF-alpha-dependent manner.
Interacts (via Ras-GAP domain) with RIPK1 (via kinase domain); the interaction occurs in a TNF-alpha-dependent manner (By similarity).
Interacts with DAB1 and DAB2.
By similarity1 PublicationGO - Molecular functioni
- 14-3-3 protein binding Source: RGD
- death receptor binding Source: RGD
- identical protein binding Source: RGD
- kinase binding Source: RGD
- mitogen-activated protein kinase kinase binding Source: RGD
- mitogen-activated protein kinase kinase kinase binding Source: RGD
- phosphatidylinositol 3-kinase binding Source: UniProtKB
- phosphatidylinositol 3-kinase regulatory subunit binding Source: UniProtKB
- protein homodimerization activity Source: RGD
- protein kinase binding Source: RGD
- protein N-terminus binding Source: RGD
- protein phosphatase 2A binding Source: RGD
- SH3 domain binding Source: UniProtKB
- vascular endothelial growth factor receptor 2 binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 251329, 1 interactor |
STRINGi | 10116.ENSRNOP00000056740 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 118 | C2PROSITE-ProRule annotationAdd BLAST | 118 | |
Domaini | 178 – 370 | Ras-GAPPROSITE-ProRule annotationAdd BLAST | 193 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 453 – 750 | Necessary for interaction with AKT1By similarityAdd BLAST | 298 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 832 – 966 | Sequence analysisAdd BLAST | 135 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 674 – 677 | Poly-Ala | 4 | |
Compositional biasi | 710 – 755 | Pro-richAdd BLAST | 46 |
Domaini
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG3508, Eukaryota |
GeneTreei | ENSGT00940000155853 |
InParanoidi | Q6P730 |
OrthoDBi | 69536at2759 |
Family and domain databases
Gene3Di | 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR000008, C2_dom IPR035892, C2_domain_sf IPR030403, DAB2IP IPR021887, DUF3498 IPR039360, Ras_GTPase IPR023152, RasGAP_CS IPR001936, RasGAP_dom IPR008936, Rho_GTPase_activation_prot |
PANTHERi | PTHR10194, PTHR10194, 1 hit PTHR10194:SF26, PTHR10194:SF26, 1 hit |
Pfami | View protein in Pfam PF00168, C2, 1 hit PF12004, DUF3498, 1 hit PF00616, RasGAP, 2 hits |
SMARTi | View protein in SMART SM00239, C2, 1 hit SM00323, RasGAP, 1 hit |
SUPFAMi | SSF48350, SSF48350, 1 hit |
PROSITEi | View protein in PROSITE PS50004, C2, 1 hit PS00509, RAS_GTPASE_ACTIV_1, 1 hit PS50018, RAS_GTPASE_ACTIV_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MENLRRAVHP NKDNSRRVEH ILKLWVIEAK DLPAKKKYLC ELCLDDVLYA
60 70 80 90 100
RTTGKLKTDN VFWGEHFEFH NLPPLRTVTV HLYRETDKKK KKERNSYLGL
110 120 130 140 150
VSLPAASVAG RQFVEKWYPV VTPNPKGGKG PGPMIRIKAR YQTITILPME
160 170 180 190 200
MYKEFAEHIT NHYLGLCAAL EPILSAKTKE EMASALVHIL QSTGKVKDFL
210 220 230 240 250
TDLMMSEVDR CGDNEHLIFR ENTLATKAIE EYLKLVGQKY LQDALGEFIK
260 270 280 290 300
ALYESDENCE VDPSKCSAAD LPEHQGNLKM CCELAFCKII NSYCVFPREL
310 320 330 340 350
KEVFASWRQE CSSRGRPDIS ERLISASLFL RFLCPAIMSP SLFNLLQEYP
360 370 380 390 400
DDRTARTLTL IAKVTQNLAN FAKFGSKEEY MSFMNQFLEH EWTNMQRFLL
410 420 430 440 450
EISNPETLSN TAGFEGYIDL GRELSSLHSL LWEAVSQLDQ SIVSKLGPLP
460 470 480 490 500
RILRDVHTAL STPGSGQLPG TNDLASTPGS GSSSVSAGLQ KMVIENDLSG
510 520 530 540 550
LIDFTRLPSP TPENKDLFFV TRSSGVQPSP ARSSSYSEAN EPDLQMANGS
560 570 580 590 600
KSLSMVDLQD ARTLDGEAGS PVGPEALPAD GQVPATQLVA GWPARAAPVS
610 620 630 640 650
LAGLATVRRA VPTPTTPGTS EGAPGRPQLL APLSFQNPVY QMAAGLPLSP
660 670 680 690 700
RGLGDSGSEG HSSLSSHSNS EELAAAAKLG SFSTAAEELA RRPGELARRQ
710 720 730 740 750
MSLTEKGGQP TVPRQNSAGP QRRIDQPPPP PPPPPPAPRG RTPPTMLSTL
760 770 780 790 800
QYPRPSSGTL ASASPDWAGP GTRLRQQSSS SKGDSPELKP RALHKQGPSP
810 820 830 840 850
VSPNALDRTA AWLLTMNAQL LEDEGLGPDP PHRDRLRSKE ELSQAEKDLA
860 870 880 890 900
VLQDKLRIST KKLEEYETLF KCQEETTQKL VLEYQARLEE GEERLRRQQE
910 920 930 940 950
DKDVQMKGII SRLMSVEEEL KKDHAEMQAA VDSKQKIIDA QEKRIASLDA
960 970 980 990
ANARLMSALT QLKERYSMRA RNGVSPTNPT KLQITENGEF RNSSNC
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JTF2 | A0A0G2JTF2_RAT | Disabled homolog 2-interacting prot... | Dab2ip | 1,161 | Annotation score: | ||
A0A0G2JTS0 | A0A0G2JTS0_RAT | Disabled homolog 2-interacting prot... | Dab2ip | 1,064 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 228 | A → G in AAK93947 (PubMed:11812785).Curated | 1 | |
Sequence conflicti | 238 | Q → H in AAK93947 (PubMed:11812785).Curated | 1 | |
Sequence conflicti | 246 | G → C in AAK93947 (PubMed:11812785).Curated | 1 | |
Sequence conflicti | 487 | A → T in AAK93947 (PubMed:11812785).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC061865 mRNA Translation: AAH61865.1 AF236130 mRNA Translation: AAK93947.1 |
RefSeqi | NP_619724.3, NM_138710.3 |
Genome annotation databases
Ensembli | ENSRNOT00000083134; ENSRNOP00000072901; ENSRNOG00000055226 |
GeneIDi | 192126 |
KEGGi | rno:192126 |
UCSCi | RGD:621686, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC061865 mRNA Translation: AAH61865.1 AF236130 mRNA Translation: AAK93947.1 |
RefSeqi | NP_619724.3, NM_138710.3 |
3D structure databases
SMRi | Q6P730 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 251329, 1 interactor |
STRINGi | 10116.ENSRNOP00000056740 |
PTM databases
iPTMneti | Q6P730 |
PhosphoSitePlusi | Q6P730 |
Proteomic databases
jPOSTi | Q6P730 |
PaxDbi | Q6P730 |
PRIDEi | Q6P730 |
Genome annotation databases
Ensembli | ENSRNOT00000083134; ENSRNOP00000072901; ENSRNOG00000055226 |
GeneIDi | 192126 |
KEGGi | rno:192126 |
UCSCi | RGD:621686, rat |
Organism-specific databases
CTDi | 153090 |
RGDi | 621686, Dab2ip |
Phylogenomic databases
eggNOGi | KOG3508, Eukaryota |
GeneTreei | ENSGT00940000155853 |
InParanoidi | Q6P730 |
OrthoDBi | 69536at2759 |
Enzyme and pathway databases
Reactomei | R-RNO-5658442, Regulation of RAS by GAPs |
Miscellaneous databases
PROi | PR:Q6P730 |
Gene expression databases
Bgeei | ENSRNOG00000055226, Expressed in skeletal muscle tissue and 20 other tissues |
ExpressionAtlasi | Q6P730, baseline and differential |
Genevisiblei | Q6P730, RN |
Family and domain databases
Gene3Di | 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR000008, C2_dom IPR035892, C2_domain_sf IPR030403, DAB2IP IPR021887, DUF3498 IPR039360, Ras_GTPase IPR023152, RasGAP_CS IPR001936, RasGAP_dom IPR008936, Rho_GTPase_activation_prot |
PANTHERi | PTHR10194, PTHR10194, 1 hit PTHR10194:SF26, PTHR10194:SF26, 1 hit |
Pfami | View protein in Pfam PF00168, C2, 1 hit PF12004, DUF3498, 1 hit PF00616, RasGAP, 2 hits |
SMARTi | View protein in SMART SM00239, C2, 1 hit SM00323, RasGAP, 1 hit |
SUPFAMi | SSF48350, SSF48350, 1 hit |
PROSITEi | View protein in PROSITE PS50004, C2, 1 hit PS00509, RAS_GTPASE_ACTIV_1, 1 hit PS50018, RAS_GTPASE_ACTIV_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DAB2P_RAT | |
Accessioni | Q6P730Primary (citable) accession number: Q6P730 Secondary accession number(s): Q924M9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |