UniProtKB - Q6P5D4 (CP135_MOUSE)
Protein
Centrosomal protein of 135 kDa
Gene
Cep135
Organism
Mus musculus (Mouse)
Status
Functioni
Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner (By similarity).By similarity
GO - Molecular functioni
- protein C-terminus binding Source: MGI
GO - Biological processi
- centriole-centriole cohesion Source: MGI
- centriole replication Source: MGI
- positive regulation of establishment of protein localization Source: UniProtKB
- positive regulation of non-motile cilium assembly Source: UniProtKB
Enzyme and pathway databases
Reactomei | R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-380259, Loss of Nlp from mitotic centrosomes R-MMU-380270, Recruitment of mitotic centrosome proteins and complexes R-MMU-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-MMU-380320, Recruitment of NuMA to mitotic centrosomes R-MMU-5620912, Anchoring of the basal body to the plasma membrane R-MMU-8854518, AURKA Activation by TPX2 |
Names & Taxonomyi
Protein namesi | Recommended name: Centrosomal protein of 135 kDaShort name: Cep135 Alternative name(s): Centrosomal protein 4 |
Gene namesi | Name:Cep135 Synonyms:Cep4, Kiaa0635 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2681869, Cep135 |
Subcellular locationi
Cytoskeleton
- centriole 1 Publication
Note: During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.By similarity
Cytoskeleton
- centriole Source: UniProtKB
- centrosome Source: MGI
Other locations
- cytoplasm Source: UniProtKB-KW
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000089492 | 1 – 1140 | Centrosomal protein of 135 kDaAdd BLAST | 1140 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 439 | PhosphoserineBy similarity | 1 | |
Modified residuei | 688 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1121 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1130 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1134 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q6P5D4 |
jPOSTi | Q6P5D4 |
MaxQBi | Q6P5D4 |
PaxDbi | Q6P5D4 |
PRIDEi | Q6P5D4 |
PTM databases
iPTMneti | Q6P5D4 |
PhosphoSitePlusi | Q6P5D4 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000036403, Expressed in forelimb bud and 139 other tissues |
Genevisiblei | Q6P5D4, MM |
Interactioni
Subunit structurei
Interacts with CEP250 (By similarity).
Interacts with DCTN2.
By similarity1 PublicationGO - Molecular functioni
- protein C-terminus binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 238025, 73 interactors |
IntActi | Q6P5D4, 68 interactors |
STRINGi | 10090.ENSMUSP00000112602 |
Miscellaneous databases
RNActi | Q6P5D4, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 125 – 153 | Sequence analysisAdd BLAST | 29 | |
Coiled coili | 194 – 420 | Sequence analysisAdd BLAST | 227 | |
Coiled coili | 447 – 647 | Sequence analysisAdd BLAST | 201 | |
Coiled coili | 674 – 1117 | Sequence analysisAdd BLAST | 444 |
Sequence similaritiesi
Belongs to the CEP135/TSGA10 family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QT27, Eukaryota |
GeneTreei | ENSGT00940000159453 |
HOGENOMi | CLU_008607_0_0_1 |
InParanoidi | Q6P5D4 |
OMAi | RSLDDYQ |
OrthoDBi | 139407at2759 |
PhylomeDBi | Q6P5D4 |
TreeFami | TF326518 |
Family and domain databases
InterProi | View protein in InterPro IPR026732, Cep135 |
PANTHERi | PTHR23159:SF18, PTHR23159:SF18, 1 hit |
i Sequence
Sequence statusi: Complete.
Q6P5D4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTAAERKYI NIRKRLDQLG YRQTLSVDSL PLVEKLFSDL VHTTESLRQC
60 70 80 90 100
RLSSGKAEKE SANLDFVLEP YKLENTRLNK ENNELYLELM KLRECSDKHI
110 120 130 140 150
KDLKTTLKKC SRETADLKFL NNQYVHKVKV LEKESKAKDE KIQQLQEKNL
160 170 180 190 200
RAVVQTPGGR KRNIAFRRQR MQIDEPAPPS EVSAYPVPQP EDPYIADLLQ
210 220 230 240 250
VADNRIQELQ EEVQQLQEKL AQMEKGVLDY SKQIELRERE IQRLSLALDG
260 270 280 290 300
GCSPDVLSLE TRNKTNEKLI AHLNVQVDFL QQANKELEKH IQELMETKET
310 320 330 340 350
VTTEVVNLSN RNEKLCQELT EIDQLAQRLE RHKEQVLETA DKELGEAKKE
360 370 380 390 400
IKRNLCEMRN LEEKMSKLQW ELDLSHKEKE RLNSELLLKS DLETVVHQLE
410 420 430 440 450
QEKQRLSKKL QSFAVTEREL TLEVERMRLE HGIKRRDKSP SRLDTFLKGI
460 470 480 490 500
EEERDYYKKE LEKLQHLIQR RSCAINYSAR EKPPVVKCSE KGDCSTDVHL
510 520 530 540 550
ITRERDELQR MLERFEKYME DIQSNVKLLT AERDKLNVLY KEAKEELSTL
560 570 580 590 600
RKESTNSTSP NHLVSCVEKE KERALSELRR ITAEKEALRE KLKNIQERNA
610 620 630 640 650
VGKSDLEKTI EHLTYINHQL ENEKYELQSK MLMMKETVES LENKSKLQAQ
660 670 680 690 700
KLSHVTGDSS HQKTEMTSLR IVSEQLQRSL DDCQHRLSIK RGELESAQEQ
710 720 730 740 750
IKMLEQKLEN LSHRMTVQSE ETHAMKKTIG VMDKEKDFLQ ETVDEKTEKI
760 770 780 790 800
ANLQESLLSK EKVIAQLKVT VAEYETSLNQ LQETLTTRDR EINSLRRQLD
810 820 830 840 850
ASHKELDDVG KSREISFKEN RRLQDDLATM ARENQEISLE LEAAVQEKEE
860 870 880 890 900
MKSRVHKYIT EVSRWESLMA AKEKENKDLL DRFQMLHSRA EDWEVKAQQA
910 920 930 940 950
EGENSSVRLE LLSIDTERRH LRERVDLLEK EIQEHINAHH AYESQISSMA
960 970 980 990 1000
KAMSQLEEEL RRHESEKATM LGDVSSLREL CIKLDSGKDV MTQQLNSKSL
1010 1020 1030 1040 1050
ELERAVAELE NVKSESELLK KQLTNERQTI KNLESLLATN RDKEFQSHLT
1060 1070 1080 1090 1100
SHEKDTEIQL LKEKLNLSES KLTTQSRETS MLRTKVTQLQ TDYDNLKRQM
1110 1120 1130 1140
SNEKYERERA IQEMRRLGLP TSPLSSTLKS PVQTPDHINA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC062951 mRNA Translation: AAH62951.1 AK172988 mRNA Translation: BAD32266.1 |
CCDSi | CCDS19365.1 |
RefSeqi | NP_950197.1, NM_199032.2 XP_006535086.1, XM_006535023.3 |
Genome annotation databases
Ensembli | ENSMUST00000049060; ENSMUSP00000038674; ENSMUSG00000036403 ENSMUST00000121979; ENSMUSP00000112602; ENSMUSG00000036403 |
GeneIDi | 381644 |
KEGGi | mmu:381644 |
UCSCi | uc008xvc.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC062951 mRNA Translation: AAH62951.1 AK172988 mRNA Translation: BAD32266.1 |
CCDSi | CCDS19365.1 |
RefSeqi | NP_950197.1, NM_199032.2 XP_006535086.1, XM_006535023.3 |
3D structure databases
SMRi | Q6P5D4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 238025, 73 interactors |
IntActi | Q6P5D4, 68 interactors |
STRINGi | 10090.ENSMUSP00000112602 |
PTM databases
iPTMneti | Q6P5D4 |
PhosphoSitePlusi | Q6P5D4 |
Proteomic databases
EPDi | Q6P5D4 |
jPOSTi | Q6P5D4 |
MaxQBi | Q6P5D4 |
PaxDbi | Q6P5D4 |
PRIDEi | Q6P5D4 |
Protocols and materials databases
Antibodypediai | 23978, 134 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000049060; ENSMUSP00000038674; ENSMUSG00000036403 ENSMUST00000121979; ENSMUSP00000112602; ENSMUSG00000036403 |
GeneIDi | 381644 |
KEGGi | mmu:381644 |
UCSCi | uc008xvc.1, mouse |
Organism-specific databases
CTDi | 9662 |
MGIi | MGI:2681869, Cep135 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | ENOG502QT27, Eukaryota |
GeneTreei | ENSGT00940000159453 |
HOGENOMi | CLU_008607_0_0_1 |
InParanoidi | Q6P5D4 |
OMAi | RSLDDYQ |
OrthoDBi | 139407at2759 |
PhylomeDBi | Q6P5D4 |
TreeFami | TF326518 |
Enzyme and pathway databases
Reactomei | R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-380259, Loss of Nlp from mitotic centrosomes R-MMU-380270, Recruitment of mitotic centrosome proteins and complexes R-MMU-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-MMU-380320, Recruitment of NuMA to mitotic centrosomes R-MMU-5620912, Anchoring of the basal body to the plasma membrane R-MMU-8854518, AURKA Activation by TPX2 |
Miscellaneous databases
BioGRID-ORCSi | 381644, 3 hits in 17 CRISPR screens |
ChiTaRSi | Cep135, mouse |
PROi | PR:Q6P5D4 |
RNActi | Q6P5D4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036403, Expressed in forelimb bud and 139 other tissues |
Genevisiblei | Q6P5D4, MM |
Family and domain databases
InterProi | View protein in InterPro IPR026732, Cep135 |
PANTHERi | PTHR23159:SF18, PTHR23159:SF18, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CP135_MOUSE | |
Accessioni | Q6P5D4Primary (citable) accession number: Q6P5D4 Secondary accession number(s): Q6A030 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2005 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families