UniProtKB - Q6P4S6 (SIK3_MOUSE)
Serine/threonine-protein kinase SIK3
Sik3
Functioni
Catalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 37 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 130 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 14 – 22 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine kinase activity Source: UniProtKB-EC
- protein threonine kinase activity Source: UniProtKB-EC
- tau-protein kinase activity Source: GO_Central
GO - Biological processi
- cartilage development involved in endochondral bone morphogenesis Source: MGI
- endochondral ossification Source: MGI
- intracellular signal transduction Source: GO_Central
- limb morphogenesis Source: MGI
- microtubule cytoskeleton organization Source: GO_Central
- multicellular organism growth Source: MGI
- positive regulation of TORC1 signaling Source: UniProtKB
- positive regulation of TORC2 signaling Source: UniProtKB
- protein phosphorylation Source: UniProtKB
- regulation of protein localization Source: MGI
- skeletal system morphogenesis Source: MGI
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase SIK3 (EC:2.7.11.1)Alternative name(s): Salt-inducible kinase 3 Short name: SIK-3 Serine/threonine-protein kinase QSK |
Gene namesi | Name:Sik3 Synonyms:Kiaa0999, Qsk |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2446296, Sik3 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000252258 | 1 – 1311 | Serine/threonine-protein kinase SIK3Add BLAST | 1311 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 13 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 163 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 411 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 493 | PhosphoserineCombined sources | 1 | |
Modified residuei | 533 | PhosphoserineCombined sources | 1 | |
Modified residuei | 534 | PhosphoserineCombined sources | 1 | |
Modified residuei | 616 | PhosphoserineCombined sources | 1 | |
Modified residuei | 637 | PhosphoserineCombined sources | 1 | |
Modified residuei | 856 | PhosphoserineBy similarity | 1 | |
Modified residuei | 968 | PhosphoserineBy similarity | 1 | |
Modified residuei | 976 | Omega-N-methylarginineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | Q6P4S6 |
jPOSTi | Q6P4S6 |
PaxDbi | Q6P4S6 |
PeptideAtlasi | Q6P4S6 |
PRIDEi | Q6P4S6 |
PTM databases
iPTMneti | Q6P4S6 |
PhosphoSitePlusi | Q6P4S6 |
Expressioni
Tissue specificityi
Developmental stagei
Interactioni
Subunit structurei
Binds to and is activated by YWHAZ when phosphorylated on Thr-163 (By similarity).
Interacts with 14-3-3 proteins (By similarity).
Interacts with HDAC4; this interaction leads to HDAC4 retention in the cytoplasm (PubMed:22318228).
Interacts with DEPTOR, MLST8/GbetaL, RICTOR and RPTOR (By similarity).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 214193, 6 interactors |
IntActi | Q6P4S6, 5 interactors |
STRINGi | 10090.ENSMUSP00000121032 |
Miscellaneous databases
RNActi | Q6P4S6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 259 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 252 | |
Domaini | 286 – 326 | UBAPROSITE-ProRule annotationAdd BLAST | 41 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 640 – 1003 | Gln-richAdd BLAST | 364 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0586, Eukaryota |
InParanoidi | Q6P4S6 |
OrthoDBi | 1127668at2759 |
PhylomeDBi | Q6P4S6 |
Family and domain databases
CDDi | cd14071, STKc_SIK, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS IPR034672, SIK IPR015940, UBA |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50030, UBA, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAARIGYYEI DRTIGKGNFA VVKRATHLVT KAKVAIKIID KSQLDEENLK
60 70 80 90 100
KIFREVQIMK MLCHPHIIRL YQVMETERMI YLVTEYASGG EIFDHLVAHG
110 120 130 140 150
RMAEKEARRK FKQIVTAVYF CHCRNIVHRD LKAENLLLDA NLNIKIADFG
160 170 180 190 200
FSNLFTPGQL LKTWCGSPPY AAPELFEGKE YDGPKVDIWS LGVVLYVLVC
210 220 230 240 250
GALPFDGSTL QNLRARVLSG KFRIPFFMST ECEHLIRHML VLDPNKRLSM
260 270 280 290 300
EQICRHKWMK LGDADPNFDR LIAECQQLKE ERQSDPLNDD VLLAMEDMGL
310 320 330 340 350
DKERTLQSLR SDAYDHYSAI YSLLCDRHKK HKTLRPGALP SMPQAMTFQA
360 370 380 390 400
PVNLQAEQTG TAMNLSVPQV QLINPENQII EPDGAVNLDS DEGEEPSPEA
410 420 430 440 450
LVRYLSMRRH TVGVADPRTE VMEDLQKLLP GFPGVNPQGP FLQVAPNMNF
460 470 480 490 500
THNLLPMQSL QPTGQLEYKE QSLLQPPTLQ LLNGMGPLGR RASDGGANIQ
510 520 530 540 550
LHAQQLLKRP RGPSPLVTMT PAVPAVTPVD EESSDGEPDQ EAVQRYLANR
560 570 580 590 600
SKRHTLAMTS PTAEIPPDLQ RQLGQQSFRS RVWPPHLVPD QHRSTYKDSN
610 620 630 640 650
TLHLPTERFS PVRRFSDGAA SIQAFKAHLE KMGNSSSIKQ LQQECEQLQK
660 670 680 690 700
MYGGQVDERT LEKTQQQHML YQQEQHHQIL QQQIQDSICP PQPSPPLQVA
710 720 730 740 750
CENQPALLTH QLQRLRIQPS SPPPNHPSNH LFRQPSNSPP PVSSAMITSH
760 770 780 790 800
GATSPSQFQG LPSHGAIFQQ QPENCSPPPS VALTCLGLQQ ASQSQPVTIQ
810 820 830 840 850
LQEPVDMLSN MAGTAAGSAG RSIPISPSAS QIQIQHRASL MAPFSYGHRP
860 870 880 890 900
LSKQLSADSA EAHSLNMNRF SPANYDQAHL HPHLFSDQSR GSPSSYSPST
910 920 930 940 950
GVGFPPTQAL KVPPLDQFPT FPPSAQQQPP HYTTSALQQA LLSPTPPDYP
960 970 980 990 1000
RHQQVPHILQ GLLSPRHSLT GHSDIRLPPA EFAQLIKRQQ QHRQQQQQQQ
1010 1020 1030 1040 1050
QQQEYHELFR HMNQGDAVSL APSLGGQNMT EHQALSYQNA DSYHRHHTSP
1060 1070 1080 1090 1100
QHILQIRAQD CISQGPSPTP THGYAHQPPL MHSESMEEDC LCEGLKEGFP
1110 1120 1130 1140 1150
DKSSSTLTKG CHNSPLLLCT SGPGDPEPLL GTVSQARELG IHPYGHQPTA
1160 1170 1180 1190 1200
TTFSRNKVPS RESVLGNCLE RSSPGQAMEL PDHNGLGYPV RPLVSEHLRS
1210 1220 1230 1240 1250
RTLQRHHTIQ NSDDAYVQLD TLPGMSLVAG KALSSARMSD AVLSQSSLMG
1260 1270 1280 1290 1300
SQQFQDEEDE ECGVSLGHEH PGLGDGSQHL NSSRYPATCV TDIMLSHKHP
1310
EVSFSMEQAG V
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PU87 | E9PU87_MOUSE | Non-specific serine/threonine prote... | Sik3 | 1,369 | Annotation score: | ||
F6S7W6 | F6S7W6_MOUSE | Non-specific serine/threonine prote... | Sik3 | 1,319 | Annotation score: | ||
F6U6U5 | F6U6U5_MOUSE | Serine/threonine-protein kinase SIK... | Sik3 | 1,214 | Annotation score: | ||
F6U8X4 | F6U8X4_MOUSE | Serine/threonine-protein kinase SIK... | Sik3 | 506 | Annotation score: | ||
F6SBP3 | F6SBP3_MOUSE | Serine/threonine-protein kinase SIK... | Sik3 | 71 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1032 | H → Q in AAH82313 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1032 | H → Q in BAC98067 (PubMed:14621295).Curated | 1 | |
Sequence conflicti | 1277 | S → N in AAH63268 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1277 | S → N in AAH80688 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020894 | 1 – 58 | Missing in isoform 2. 1 PublicationAdd BLAST | 58 | |
Alternative sequenceiVSP_020895 | 546 – 1311 | Missing in isoform 2. 1 PublicationAdd BLAST | 766 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK017789 mRNA Translation: BAB30934.1 BC033915 mRNA Translation: AAH33915.1 BC063268 mRNA Translation: AAH63268.2 BC080688 mRNA Translation: AAH80688.1 BC082313 mRNA Translation: AAH82313.1 AK129257 mRNA Translation: BAC98067.1 |
RefSeqi | NP_081774.3, NM_027498.3 |
Genome annotation databases
GeneIDi | 70661 |
KEGGi | mmu:70661 |
UCSCi | uc009pha.1, mouse [Q6P4S6-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK017789 mRNA Translation: BAB30934.1 BC033915 mRNA Translation: AAH33915.1 BC063268 mRNA Translation: AAH63268.2 BC080688 mRNA Translation: AAH80688.1 BC082313 mRNA Translation: AAH82313.1 AK129257 mRNA Translation: BAC98067.1 |
RefSeqi | NP_081774.3, NM_027498.3 |
3D structure databases
SMRi | Q6P4S6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 214193, 6 interactors |
IntActi | Q6P4S6, 5 interactors |
STRINGi | 10090.ENSMUSP00000121032 |
PTM databases
iPTMneti | Q6P4S6 |
PhosphoSitePlusi | Q6P4S6 |
Proteomic databases
EPDi | Q6P4S6 |
jPOSTi | Q6P4S6 |
PaxDbi | Q6P4S6 |
PeptideAtlasi | Q6P4S6 |
PRIDEi | Q6P4S6 |
Genome annotation databases
GeneIDi | 70661 |
KEGGi | mmu:70661 |
UCSCi | uc009pha.1, mouse [Q6P4S6-2] |
Organism-specific databases
CTDi | 23387 |
MGIi | MGI:2446296, Sik3 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG0586, Eukaryota |
InParanoidi | Q6P4S6 |
OrthoDBi | 1127668at2759 |
PhylomeDBi | Q6P4S6 |
Miscellaneous databases
BioGRID-ORCSi | 70661, 3 hits in 18 CRISPR screens |
ChiTaRSi | Sik3, mouse |
PROi | PR:Q6P4S6 |
RNActi | Q6P4S6, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd14071, STKc_SIK, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS IPR034672, SIK IPR015940, UBA |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50030, UBA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SIK3_MOUSE | |
Accessioni | Q6P4S6Primary (citable) accession number: Q6P4S6 Secondary accession number(s): Q641L5 Q9CYD5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2006 |
Last sequence update: | October 3, 2006 | |
Last modified: | February 10, 2021 | |
This is version 134 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families