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Protein

Serine/threonine-protein kinase SIK3

Gene

Sik3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Activity regulationi

Activated by phosphorylation on Thr-163.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37ATPPROSITE-ProRule annotation1
Active sitei130Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 22ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB
  • tau-protein kinase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase SIK3 (EC:2.7.11.1)
Alternative name(s):
Salt-inducible kinase 3
Short name:
SIK-3
Serine/threonine-protein kinase QSK
Gene namesi
Name:Sik3
Synonyms:Kiaa0999, Qsk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2446296 Sik3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002522581 – 1311Serine/threonine-protein kinase SIK3Add BLAST1311

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphothreonineBy similarity1
Modified residuei163PhosphothreonineCombined sources1
Modified residuei411PhosphothreonineBy similarity1
Modified residuei493PhosphoserineCombined sources1
Modified residuei533PhosphoserineCombined sources1
Modified residuei534PhosphoserineCombined sources1
Modified residuei616PhosphoserineCombined sources1
Modified residuei637PhosphoserineCombined sources1
Modified residuei856PhosphoserineBy similarity1
Modified residuei968PhosphoserineBy similarity1
Modified residuei976Omega-N-methylarginineCombined sources1

Post-translational modificationi

Phosphorylated at Thr-163 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ6P4S6
PaxDbiQ6P4S6
PeptideAtlasiQ6P4S6
PRIDEiQ6P4S6

PTM databases

iPTMnetiQ6P4S6
PhosphoSitePlusiQ6P4S6

Expressioni

Gene expression databases

CleanExiMM_BC033915

Interactioni

Subunit structurei

Binds to and is activated by YWHAZ when phosphorylated on Thr-163.By similarity

Protein-protein interaction databases

BioGridi214193, 5 interactors
IntActiQ6P4S6, 5 interactors
STRINGi10090.ENSMUSP00000121032

Structurei

3D structure databases

ProteinModelPortaliQ6P4S6
SMRiQ6P4S6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 259Protein kinasePROSITE-ProRule annotationAdd BLAST252
Domaini286 – 326UBAPROSITE-ProRule annotationAdd BLAST41

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi640 – 1003Gln-richAdd BLAST364

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0586 Eukaryota
ENOG410XNQ0 LUCA
HOGENOMiHOG000154056
HOVERGENiHBG079682
InParanoidiQ6P4S6
KOiK19009
PhylomeDBiQ6P4S6

Family and domain databases

CDDicd14071 STKc_SIK, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
IPR034672 SIK
IPR015940 UBA
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50030 UBA, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q6P4S6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAARIGYYEI DRTIGKGNFA VVKRATHLVT KAKVAIKIID KSQLDEENLK
60 70 80 90 100
KIFREVQIMK MLCHPHIIRL YQVMETERMI YLVTEYASGG EIFDHLVAHG
110 120 130 140 150
RMAEKEARRK FKQIVTAVYF CHCRNIVHRD LKAENLLLDA NLNIKIADFG
160 170 180 190 200
FSNLFTPGQL LKTWCGSPPY AAPELFEGKE YDGPKVDIWS LGVVLYVLVC
210 220 230 240 250
GALPFDGSTL QNLRARVLSG KFRIPFFMST ECEHLIRHML VLDPNKRLSM
260 270 280 290 300
EQICRHKWMK LGDADPNFDR LIAECQQLKE ERQSDPLNDD VLLAMEDMGL
310 320 330 340 350
DKERTLQSLR SDAYDHYSAI YSLLCDRHKK HKTLRPGALP SMPQAMTFQA
360 370 380 390 400
PVNLQAEQTG TAMNLSVPQV QLINPENQII EPDGAVNLDS DEGEEPSPEA
410 420 430 440 450
LVRYLSMRRH TVGVADPRTE VMEDLQKLLP GFPGVNPQGP FLQVAPNMNF
460 470 480 490 500
THNLLPMQSL QPTGQLEYKE QSLLQPPTLQ LLNGMGPLGR RASDGGANIQ
510 520 530 540 550
LHAQQLLKRP RGPSPLVTMT PAVPAVTPVD EESSDGEPDQ EAVQRYLANR
560 570 580 590 600
SKRHTLAMTS PTAEIPPDLQ RQLGQQSFRS RVWPPHLVPD QHRSTYKDSN
610 620 630 640 650
TLHLPTERFS PVRRFSDGAA SIQAFKAHLE KMGNSSSIKQ LQQECEQLQK
660 670 680 690 700
MYGGQVDERT LEKTQQQHML YQQEQHHQIL QQQIQDSICP PQPSPPLQVA
710 720 730 740 750
CENQPALLTH QLQRLRIQPS SPPPNHPSNH LFRQPSNSPP PVSSAMITSH
760 770 780 790 800
GATSPSQFQG LPSHGAIFQQ QPENCSPPPS VALTCLGLQQ ASQSQPVTIQ
810 820 830 840 850
LQEPVDMLSN MAGTAAGSAG RSIPISPSAS QIQIQHRASL MAPFSYGHRP
860 870 880 890 900
LSKQLSADSA EAHSLNMNRF SPANYDQAHL HPHLFSDQSR GSPSSYSPST
910 920 930 940 950
GVGFPPTQAL KVPPLDQFPT FPPSAQQQPP HYTTSALQQA LLSPTPPDYP
960 970 980 990 1000
RHQQVPHILQ GLLSPRHSLT GHSDIRLPPA EFAQLIKRQQ QHRQQQQQQQ
1010 1020 1030 1040 1050
QQQEYHELFR HMNQGDAVSL APSLGGQNMT EHQALSYQNA DSYHRHHTSP
1060 1070 1080 1090 1100
QHILQIRAQD CISQGPSPTP THGYAHQPPL MHSESMEEDC LCEGLKEGFP
1110 1120 1130 1140 1150
DKSSSTLTKG CHNSPLLLCT SGPGDPEPLL GTVSQARELG IHPYGHQPTA
1160 1170 1180 1190 1200
TTFSRNKVPS RESVLGNCLE RSSPGQAMEL PDHNGLGYPV RPLVSEHLRS
1210 1220 1230 1240 1250
RTLQRHHTIQ NSDDAYVQLD TLPGMSLVAG KALSSARMSD AVLSQSSLMG
1260 1270 1280 1290 1300
SQQFQDEEDE ECGVSLGHEH PGLGDGSQHL NSSRYPATCV TDIMLSHKHP
1310
EVSFSMEQAG V
Length:1,311
Mass (Da):145,784
Last modified:October 3, 2006 - v3
Checksum:iCF110B679B4C3F2B
GO
Isoform 2 (identifier: Q6P4S6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-58: Missing.
     546-1311: Missing.

Note: No experimental confirmation available.
Show »
Length:487
Mass (Da):54,776
Checksum:i5FB269344262D4F2
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PU87E9PU87_MOUSE
Serine/threonine-protein kinase SIK...
Sik3
1,369Annotation score:
F6S7W6F6S7W6_MOUSE
Serine/threonine-protein kinase SIK...
Sik3
1,319Annotation score:
F6U6U5F6U6U5_MOUSE
Serine/threonine-protein kinase SIK...
Sik3
1,214Annotation score:
F6U8X4F6U8X4_MOUSE
Serine/threonine-protein kinase SIK...
Sik3
506Annotation score:
F6SBP3F6SBP3_MOUSE
Serine/threonine-protein kinase SIK...
Sik3
71Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1032H → Q in AAH82313 (PubMed:15489334).Curated1
Sequence conflicti1032H → Q in BAC98067 (PubMed:14621295).Curated1
Sequence conflicti1277S → N in AAH63268 (PubMed:15489334).Curated1
Sequence conflicti1277S → N in AAH80688 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0208941 – 58Missing in isoform 2. 1 PublicationAdd BLAST58
Alternative sequenceiVSP_020895546 – 1311Missing in isoform 2. 1 PublicationAdd BLAST766

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017789 mRNA Translation: BAB30934.1
BC033915 mRNA Translation: AAH33915.1
BC063268 mRNA Translation: AAH63268.2
BC080688 mRNA Translation: AAH80688.1
BC082313 mRNA Translation: AAH82313.1
AK129257 mRNA Translation: BAC98067.1
RefSeqiNP_081774.3, NM_027498.3
UniGeneiMm.219459

Genome annotation databases

GeneIDi70661
KEGGimmu:70661
UCSCiuc009pha.1 mouse [Q6P4S6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017789 mRNA Translation: BAB30934.1
BC033915 mRNA Translation: AAH33915.1
BC063268 mRNA Translation: AAH63268.2
BC080688 mRNA Translation: AAH80688.1
BC082313 mRNA Translation: AAH82313.1
AK129257 mRNA Translation: BAC98067.1
RefSeqiNP_081774.3, NM_027498.3
UniGeneiMm.219459

3D structure databases

ProteinModelPortaliQ6P4S6
SMRiQ6P4S6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214193, 5 interactors
IntActiQ6P4S6, 5 interactors
STRINGi10090.ENSMUSP00000121032

PTM databases

iPTMnetiQ6P4S6
PhosphoSitePlusiQ6P4S6

Proteomic databases

EPDiQ6P4S6
PaxDbiQ6P4S6
PeptideAtlasiQ6P4S6
PRIDEiQ6P4S6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi70661
KEGGimmu:70661
UCSCiuc009pha.1 mouse [Q6P4S6-2]

Organism-specific databases

CTDi23387
MGIiMGI:2446296 Sik3
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0586 Eukaryota
ENOG410XNQ0 LUCA
HOGENOMiHOG000154056
HOVERGENiHBG079682
InParanoidiQ6P4S6
KOiK19009
PhylomeDBiQ6P4S6

Miscellaneous databases

ChiTaRSiSik3 mouse
PROiPR:Q6P4S6
SOURCEiSearch...

Gene expression databases

CleanExiMM_BC033915

Family and domain databases

CDDicd14071 STKc_SIK, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
IPR034672 SIK
IPR015940 UBA
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50030 UBA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSIK3_MOUSE
AccessioniPrimary (citable) accession number: Q6P4S6
Secondary accession number(s): Q641L5
, Q66JZ5, Q6ZQ09, Q8K075, Q9CYD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 3, 2006
Last modified: November 7, 2018
This is version 121 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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