UniProtKB - Q6P4R8 (NFRKB_HUMAN)
Protein
Nuclear factor related to kappa-B-binding protein
Gene
NFRKB
Organism
Homo sapiens (Human)
Status
Functioni
Binds to the DNA consensus sequence 5'-GGGGAATCTCC-3'.1 Publication
Putative regulatory component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Modulates the deubiquitinase activity of UCHL5 in the INO80 complex.1 Publication
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- protease binding Source: UniProtKB
GO - Biological processi
- DNA recombination Source: UniProtKB-KW
- DNA repair Source: UniProtKB-KW
- protein deubiquitination Source: Reactome
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA damage, DNA recombination, DNA repair, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q6P4R8 |
Reactomei | R-HSA-5689603, UCH proteinases R-HSA-5696394, DNA Damage Recognition in GG-NER |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear factor related to kappa-B-binding proteinAlternative name(s): DNA-binding protein R kappa-B INO80 complex subunit G |
Gene namesi | Name:NFRKB Synonyms:INO80G |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7802, NFRKB |
MIMi | 164013, gene |
neXtProti | NX_Q6P4R8 |
VEuPathDBi | HostDB:ENSG00000170322.14 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- Ino80 complex Source: UniProtKB
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 4798 |
OpenTargetsi | ENSG00000170322 |
PharmGKBi | PA31606 |
Miscellaneous databases
Pharosi | Q6P4R8, Tbio |
Genetic variation databases
BioMutai | NFRKB |
DMDMi | 90101417 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000227807 | 1 – 1299 | Nuclear factor related to kappa-B-binding proteinAdd BLAST | 1299 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 228 | PhosphoserineCombined sources | 1 | |
Modified residuei | 298 | PhosphoserineCombined sources | 1 | |
Cross-linki | 327 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 351 | PhosphoserineCombined sources | 1 | |
Cross-linki | 469 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 488 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources | ||
Cross-linki | 488 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources | ||
Modified residuei | 1022 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1237 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 1291 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q6P4R8 |
jPOSTi | Q6P4R8 |
MassIVEi | Q6P4R8 |
MaxQBi | Q6P4R8 |
PaxDbi | Q6P4R8 |
PeptideAtlasi | Q6P4R8 |
PRIDEi | Q6P4R8 |
ProteomicsDBi | 66983 [Q6P4R8-1] 66984 [Q6P4R8-2] 66985 [Q6P4R8-3] |
PTM databases
CarbonylDBi | Q6P4R8 |
GlyConnecti | 2888, 1 O-Linked glycan (2 sites) |
GlyGeni | Q6P4R8, 5 sites, 1 O-linked glycan (5 sites) |
iPTMneti | Q6P4R8 |
MetOSitei | Q6P4R8 |
PhosphoSitePlusi | Q6P4R8 |
Expressioni
Tissue specificityi
Expressed in thymus, brain, testes, spleen and liver.1 Publication
Gene expression databases
Bgeei | ENSG00000170322, Expressed in buccal mucosa cell and 229 other tissues |
ExpressionAtlasi | Q6P4R8, baseline and differential |
Genevisiblei | Q6P4R8, HS |
Organism-specific databases
HPAi | ENSG00000170322, Low tissue specificity |
Interactioni
Subunit structurei
Component of the chromatin remodeling INO80 complex; specifically part of a complex module associated with the N-terminus of INO80.
Interacts with UCHL5; NFRKB competes with ADRM1 for interaction with UCHL5.
3 PublicationsBinary interactionsi
Hide detailsQ6P4R8
With | #Exp. | IntAct |
---|---|---|
UCHL5 [Q9Y5K5] | 12 | EBI-2511210,EBI-1051183 |
UCHL5 - isoform 3 [Q9Y5K5-3] | 3 | EBI-2511210,EBI-11749875 |
GO - Molecular functioni
- protease binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 110864, 57 interactors |
ComplexPortali | CPX-846, INO80 chromatin remodeling complex |
CORUMi | Q6P4R8 |
IntActi | Q6P4R8, 40 interactors |
MINTi | Q6P4R8 |
STRINGi | 9606.ENSP00000436926 |
Miscellaneous databases
RNActi | Q6P4R8, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q6P4R8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q6P4R8 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 156 | DEUBADPROSITE-ProRule annotationAdd BLAST | 118 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 370 – 495 | Winged-helix like domainAdd BLAST | 126 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 309 – 327 | Lys-richAdd BLAST | 19 | |
Compositional biasi | 665 – 672 | Poly-Ala | 8 | |
Compositional biasi | 684 – 803 | Ser-richAdd BLAST | 120 | |
Compositional biasi | 716 – 739 | Pro-richAdd BLAST | 24 | |
Compositional biasi | 1245 – 1271 | Gln-richAdd BLAST | 27 |
Domaini
NFRKB seems to be mostly disordered. The wing-helix like domain doesn't bind DNA.
Sequence similaritiesi
Belongs to the NFRKB family.Curated
Phylogenomic databases
eggNOGi | KOG1927, Eukaryota |
GeneTreei | ENSGT00390000016213 |
HOGENOMi | CLU_007638_0_0_1 |
InParanoidi | Q6P4R8 |
OMAi | VKCPPVP |
OrthoDBi | 140291at2759 |
PhylomeDBi | Q6P4R8 |
TreeFami | TF324944 |
Family and domain databases
Gene3Di | 1.10.10.2430, 1 hit |
InterProi | View protein in InterPro IPR024867, NFRKB IPR025220, NFRKB_winged_dom IPR038106, NFRKB_winged_sf |
PANTHERi | PTHR13052, PTHR13052, 1 hit |
Pfami | View protein in Pfam PF14465, NFRKB_winged, 1 hit |
PROSITEi | View protein in PROSITE PS51916, DEUBAD, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q6P4R8-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDSLDHMLTD PLELGPCGDG HGTRIMEDCL LGGTRVSLPE DLLEDPEIFF
60 70 80 90 100
DVVSLSTWQE VLSDSQREHL QQFLPQFPED SAEQQNELIL ALFSGENFRF
110 120 130 140 150
GNPLHIAQKL FRDGHFNPEV VKYRQLCFKS QYKRYLNSQQ QYFHRLLKQI
160 170 180 190 200
LASRSDLLEM ARRSGPALPF RQKRPSPSRT PEEREWRTQQ RYLKVLREVK
210 220 230 240 250
EECGDTALSS DEEDLSSWLP SSPARSPSPA VPLRVVPTLS TTDMKTADKV
260 270 280 290 300
ELGDSDLKIM LKKHHEKRKH QPDHPDLLTG DLTLNDIMTR VNAGRKGSLA
310 320 330 340 350
ALYDLAVLKK KVKEKEEKKK KKIKTIKSEA EDLAEPLSST EGVAPLSQAP
360 370 380 390 400
SPLAIPAIKE EPLEDLKPCL GINEISSSFF SLLLEILLLE SQASLPMLEE
410 420 430 440 450
RVLDWQSSPA SSLNSWFSAA PNWAELVLPA LQYLAGESRA VPSSFSPFVE
460 470 480 490 500
FKEKTQQWKL LGQSQDNEKE LAALFQLWLE TKDQAFCKQE NEDSSDATTP
510 520 530 540 550
VPRVRTDYVV RPSTGEEKRV FQEQERYRYS QPHKAFTFRM HGFESVVGPV
560 570 580 590 600
KGVFDKETSL NKAREHSLLR SDRPAYVTIL SLVRDAAARL PNGEGTRAEI
610 620 630 640 650
CELLKDSQFL APDVTSTQVN TVVSGALDRL HYEKDPCVKY DIGRKLWIYL
660 670 680 690 700
HRDRSEEEFE RIHQAQAAAA KARKALQQKP KPPSKVKSSS KESSIKVLSS
710 720 730 740 750
GPSEQSQMSL SDSSMPPTPV TPVTPTTPAL PAIPISPPPV SAVNKSGPST
760 770 780 790 800
VSEPAKSSSG VLLVSSPTMP HLGTMLSPAS SQTAPSSQAA ARVVSHSGSA
810 820 830 840 850
GLSQVRVVAQ PSLPAVPQQS GGPAQTLPQM PAGPQIRVPA TATQTKVVPQ
860 870 880 890 900
TVMATVPVKA QTTAATVQRP GPGQTGLTVT SLPATASPVS KPATSSPGTS
910 920 930 940 950
APSASTAAVI QNVTGQNIIK QVAITGQLGV KPQTGNSIPL TATNFRIQGK
960 970 980 990 1000
DVLRLPPSSI TTDAKGQTVL RITPDMMATL AKSQVTTVKL TQDLFGTGGN
1010 1020 1030 1040 1050
TTGKGISATL HVTSNPVHAA DSPAKASSAS APSSTPTGTT VVKVTPDLKP
1060 1070 1080 1090 1100
TEASSSAFRL MPALGVSVAD QKGKSTVASS EAKPAATIRI VQGLGVMPPK
1110 1120 1130 1140 1150
AGQTITVATH AKQGASVASG SGTVHTSAVS LPSMNAAVSK TVAVASGAAS
1160 1170 1180 1190 1200
TPISISTGAP TVRQVPVSTT VVSTSQAGKL PTRITVPLSV ISQPMKGKSV
1210 1220 1230 1240 1250
VTAPIIKGNL GANLSGLGRN IILTTMPAGT KLIAGNKPVS FLTAQQLQQL
1260 1270 1280 1290
QQQGQATQVR IQTVPASHLQ QGTASGSSKA VSTVVVTTAP SPKQAPEQQ
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PQ59 | E9PQ59_HUMAN | Nuclear factor-related to kappa-B-b... | NFRKB | 987 | Annotation score: | ||
E9PJG2 | E9PJG2_HUMAN | Nuclear factor-related to kappa-B-b... | NFRKB | 188 | Annotation score: | ||
E9PM88 | E9PM88_HUMAN | Nuclear factor-related to kappa-B-b... | NFRKB | 92 | Annotation score: | ||
E9PQB0 | E9PQB0_HUMAN | Nuclear factor-related to kappa-B-b... | NFRKB | 122 | Annotation score: | ||
E9PJU3 | E9PJU3_HUMAN | Nuclear factor-related to kappa-B-b... | NFRKB | 147 | Annotation score: | ||
E9PJE1 | E9PJE1_HUMAN | Nuclear factor-related to kappa-B-b... | NFRKB | 46 | Annotation score: |
Sequence cautioni
The sequence AAA17871 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1178 | G → E in AAH63280 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1213 | N → D in AAH63280 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_017592 | 1 | M → MHVFVQSTCGEETM in isoform 2. 2 Publications | 1 | |
Alternative sequenceiVSP_017593 | 213 | E → EATLDLQEQFSFE in isoform 2. 2 Publications | 1 | |
Alternative sequenceiVSP_017594 | 687 | Missing in isoform 3. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U08191 mRNA Translation: AAA17871.1 Sequence problems. X80878 mRNA Translation: CAA56846.1 BC063280 mRNA Translation: AAH63280.1 AL512730 mRNA Translation: CAC21663.1 |
CCDSi | CCDS44770.1 [Q6P4R8-1] CCDS8483.1 [Q6P4R8-2] |
PIRi | S52863 |
RefSeqi | NP_001137307.1, NM_001143835.1 [Q6P4R8-1] NP_006156.2, NM_006165.3 [Q6P4R8-2] XP_011541153.1, XM_011542851.2 [Q6P4R8-2] XP_011541154.1, XM_011542852.2 [Q6P4R8-2] XP_016873285.1, XM_017017796.1 [Q6P4R8-1] |
Genome annotation databases
Ensembli | ENST00000446488; ENSP00000400476; ENSG00000170322 [Q6P4R8-1] ENST00000524746; ENSP00000433572; ENSG00000170322 [Q6P4R8-1] ENST00000524794; ENSP00000436926; ENSG00000170322 [Q6P4R8-2] |
GeneIDi | 4798 |
KEGGi | hsa:4798 |
UCSCi | uc001qfg.4, human [Q6P4R8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U08191 mRNA Translation: AAA17871.1 Sequence problems. X80878 mRNA Translation: CAA56846.1 BC063280 mRNA Translation: AAH63280.1 AL512730 mRNA Translation: CAC21663.1 |
CCDSi | CCDS44770.1 [Q6P4R8-1] CCDS8483.1 [Q6P4R8-2] |
PIRi | S52863 |
RefSeqi | NP_001137307.1, NM_001143835.1 [Q6P4R8-1] NP_006156.2, NM_006165.3 [Q6P4R8-2] XP_011541153.1, XM_011542851.2 [Q6P4R8-2] XP_011541154.1, XM_011542852.2 [Q6P4R8-2] XP_016873285.1, XM_017017796.1 [Q6P4R8-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3U21 | X-ray | 2.18 | A/B | 370-495 | [»] | |
4UF5 | X-ray | 3.70 | B | 40-170 | [»] | |
4UF6 | X-ray | 3.69 | C/F/I/L | 40-101 | [»] | |
4WLP | X-ray | 3.10 | B | 40-153 | [»] | |
SMRi | Q6P4R8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110864, 57 interactors |
ComplexPortali | CPX-846, INO80 chromatin remodeling complex |
CORUMi | Q6P4R8 |
IntActi | Q6P4R8, 40 interactors |
MINTi | Q6P4R8 |
STRINGi | 9606.ENSP00000436926 |
PTM databases
CarbonylDBi | Q6P4R8 |
GlyConnecti | 2888, 1 O-Linked glycan (2 sites) |
GlyGeni | Q6P4R8, 5 sites, 1 O-linked glycan (5 sites) |
iPTMneti | Q6P4R8 |
MetOSitei | Q6P4R8 |
PhosphoSitePlusi | Q6P4R8 |
Genetic variation databases
BioMutai | NFRKB |
DMDMi | 90101417 |
Proteomic databases
EPDi | Q6P4R8 |
jPOSTi | Q6P4R8 |
MassIVEi | Q6P4R8 |
MaxQBi | Q6P4R8 |
PaxDbi | Q6P4R8 |
PeptideAtlasi | Q6P4R8 |
PRIDEi | Q6P4R8 |
ProteomicsDBi | 66983 [Q6P4R8-1] 66984 [Q6P4R8-2] 66985 [Q6P4R8-3] |
Protocols and materials databases
Antibodypediai | 1820, 117 antibodies |
Genome annotation databases
Ensembli | ENST00000446488; ENSP00000400476; ENSG00000170322 [Q6P4R8-1] ENST00000524746; ENSP00000433572; ENSG00000170322 [Q6P4R8-1] ENST00000524794; ENSP00000436926; ENSG00000170322 [Q6P4R8-2] |
GeneIDi | 4798 |
KEGGi | hsa:4798 |
UCSCi | uc001qfg.4, human [Q6P4R8-1] |
Organism-specific databases
CTDi | 4798 |
DisGeNETi | 4798 |
GeneCardsi | NFRKB |
HGNCi | HGNC:7802, NFRKB |
HPAi | ENSG00000170322, Low tissue specificity |
MIMi | 164013, gene |
neXtProti | NX_Q6P4R8 |
OpenTargetsi | ENSG00000170322 |
PharmGKBi | PA31606 |
VEuPathDBi | HostDB:ENSG00000170322.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1927, Eukaryota |
GeneTreei | ENSGT00390000016213 |
HOGENOMi | CLU_007638_0_0_1 |
InParanoidi | Q6P4R8 |
OMAi | VKCPPVP |
OrthoDBi | 140291at2759 |
PhylomeDBi | Q6P4R8 |
TreeFami | TF324944 |
Enzyme and pathway databases
PathwayCommonsi | Q6P4R8 |
Reactomei | R-HSA-5689603, UCH proteinases R-HSA-5696394, DNA Damage Recognition in GG-NER |
Miscellaneous databases
BioGRID-ORCSi | 4798, 311 hits in 1008 CRISPR screens |
ChiTaRSi | NFRKB, human |
EvolutionaryTracei | Q6P4R8 |
GeneWikii | NFRKB |
GenomeRNAii | 4798 |
Pharosi | Q6P4R8, Tbio |
PROi | PR:Q6P4R8 |
RNActi | Q6P4R8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000170322, Expressed in buccal mucosa cell and 229 other tissues |
ExpressionAtlasi | Q6P4R8, baseline and differential |
Genevisiblei | Q6P4R8, HS |
Family and domain databases
Gene3Di | 1.10.10.2430, 1 hit |
InterProi | View protein in InterPro IPR024867, NFRKB IPR025220, NFRKB_winged_dom IPR038106, NFRKB_winged_sf |
PANTHERi | PTHR13052, PTHR13052, 1 hit |
Pfami | View protein in Pfam PF14465, NFRKB_winged, 1 hit |
PROSITEi | View protein in PROSITE PS51916, DEUBAD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NFRKB_HUMAN | |
Accessioni | Q6P4R8Primary (citable) accession number: Q6P4R8 Secondary accession number(s): Q12869, Q15312, Q9H048 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 21, 2006 |
Last sequence update: | March 21, 2006 | |
Last modified: | April 7, 2021 | |
This is version 148 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families