UniProtKB - Q6P3N6 (CDK8_XENTR)
Protein
Cyclin-dependent kinase 8
Gene
cdk8
Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Functioni
Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex (By similarity).By similarity
Catalytic activityi
Cofactori
Mg2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 52 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 151 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 27 – 35 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
- protein kinase activity Source: UniProtKB
- RNA polymerase II CTD heptapeptide repeat kinase activity Source: GO_Central
GO - Biological processi
- protein phosphorylation Source: GO_Central
Keywordsi
Molecular function | Activator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase |
Biological process | Transcription, Transcription regulation |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-XTR-212436, Generic Transcription Pathway R-XTR-2173796, SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:cdk8 |
Organismi | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
Taxonomic identifieri | 8364 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana |
Proteomesi |
|
Organism-specific databases
Xenbasei | XB-GENE-962267, cdk8 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- mediator complex Source: GO_Central
- nucleus Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000312928 | 1 – 464 | Cyclin-dependent kinase 8Add BLAST | 464 |
Interactioni
Subunit structurei
Component of the Mediator complex.
Interacts with ccnc.
By similarityProtein-protein interaction databases
STRINGi | 8364.ENSXETP00000023365 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 335 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 315 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 15 | Interaction with CCNCBy similarityAdd BLAST | 15 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 373 – 377 | Poly-Gln | 5 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0666, Eukaryota |
InParanoidi | Q6P3N6 |
OrthoDBi | 642369at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All
Q6P3N6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDYDFKVKLT GERERVEDLF EYEGCKVGRG TYGHVYKAKR KDGRDDRDYA
60 70 80 90 100
LKQIEGTGIS MSACREIALL RELKHPNVIS LQKVFLSHAD RKVWLLFDFA
110 120 130 140 150
EHDLWHIIKF HRASKANKKP VQLPRGMVKS LLYQILDGIH YLHANWVLHR
160 170 180 190 200
DLKPANILVM GEGPERGRVK IADMGFARLF NSPLKPLADL DPVVVTFWYR
210 220 230 240 250
APELLLGARH YTKAIDIWAI GCIFAELLTS EPIFHCRQED IKTSNPYHHD
260 270 280 290 300
QLDRIFNVMG FPADKDWEDI KKMPEHSTLI KDFRRNTYTN CSLIKYMEKH
310 320 330 340 350
KVKPDSKTFH LLQKLLTMDP IKRISSEQAM QDPYFLEDPL PTSDVFAGCQ
360 370 380 390 400
IPYPKREFLT EEEPDDKGDK KNQQQQQGNN HTNGTGHPGN QDNSHAQGPP
410 420 430 440 450
LKKVRVVPPT TTSGGLIMTS DYQRSNPHAA YQNPGPSTSQ PQSSMGYSST
460
SQQPPQYSHQ THRY
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF7AFD0 | F7AFD0_XENTR | Cyclin-dependent kinase 8 | hoxa13 cdk8 | 421 | Annotation score: | ||
A0A6I8Q791 | A0A6I8Q791_XENTR | Cyclin-dependent kinase 8 | cdk8 | 495 | Annotation score: | ||
A0A6I8QW08 | A0A6I8QW08_XENTR | Cyclin-dependent kinase 8 | cdk8 | 360 | Annotation score: | ||
A0A6I8RT57 | A0A6I8RT57_XENTR | Cyclin-dependent kinase 8 | cdk8 | 423 | Annotation score: | ||
A0A6I8S0H7 | A0A6I8S0H7_XENTR | Cyclin-dependent kinase 8 | cdk8 | 411 | Annotation score: | ||
F7D567 | F7D567_XENTR | Homeobox A13 | hoxa13 | 295 | Annotation score: | ||
A0A6I8SWL6 | A0A6I8SWL6_XENTR | Cyclin-dependent kinase 8 | cdk8 | 512 | Annotation score: | ||
A0A6I8T0T0 | A0A6I8T0T0_XENTR | Cyclin-dependent kinase 8 | cdk8 | 394 | Annotation score: | ||
F7AK34 | F7AK34_XENTR | Cyclin-dependent kinase 8 | cdk8 | 350 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC063921 mRNA Translation: AAH63921.1 |
RefSeqi | NP_989246.1, NM_203915.1 |
Genome annotation databases
GeneIDi | 394856 |
KEGGi | xtr:394856 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC063921 mRNA Translation: AAH63921.1 |
RefSeqi | NP_989246.1, NM_203915.1 |
3D structure databases
SMRi | Q6P3N6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 8364.ENSXETP00000023365 |
Genome annotation databases
GeneIDi | 394856 |
KEGGi | xtr:394856 |
Organism-specific databases
CTDi | 1024 |
Xenbasei | XB-GENE-962267, cdk8 |
Phylogenomic databases
eggNOGi | KOG0666, Eukaryota |
InParanoidi | Q6P3N6 |
OrthoDBi | 642369at2759 |
Enzyme and pathway databases
Reactomei | R-XTR-212436, Generic Transcription Pathway R-XTR-2173796, SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDK8_XENTR | |
Accessioni | Q6P3N6Primary (citable) accession number: Q6P3N6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families