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Protein

Protein arginine N-methyltransferase 9

Gene

PRMT9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA). Specifically mediates the symmetrical dimethylation of SF3B2. Involved in the regulation of alternative splicing of pre-mRNA (PubMed:25737013, PubMed:25979344).2 Publications

Caution

This protein should not be confused with FBXO11 (AC Q86XK2) that was initially erroneously named PRMT9.Curated

Catalytic activityi

2 S-adenosyl-L-methionine + [protein]-L-arginine = 2 S-adenosyl-L-homocysteine + [protein]-N(omega),N(omega')-dimethyl-L-arginine.3 Publications

pH dependencei

Optimum pH is between is between 7.5 and 8.0.1 Publication

Temperature dependencei

Optimum temperature is 37 degrees Celsius.1 Publication

GO - Molecular functioni

  • histone-arginine N-methyltransferase activity Source: GO_Central
  • protein-arginine N-methyltransferase activity Source: UniProtKB
  • protein-arginine omega-N asymmetric methyltransferase activity Source: GO_Central
  • protein-arginine omega-N monomethyltransferase activity Source: GO_Central
  • protein-arginine omega-N symmetric methyltransferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein arginine N-methyltransferase 9
Alternative name(s):
Protein arginine N-methyltransferase 10 (EC:2.1.1.3203 Publications)
Gene namesi
Name:PRMT9Imported
Synonyms:PRMT10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000164169.12
HGNCiHGNC:25099 PRMT9
MIMi616125 gene
neXtProtiNX_Q6P2P2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi182 – 185LDIG → AAAA: Loss of interaction with SF3B2; Abolishes enzymatic activity. 1 Publication4
Mutagenesisi258D → G: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi260G → E: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi431C → H: 8-fold increase in MMA production and almost complete elimination of sDMA production. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000164169
PharmGKBiPA165664476

Polymorphism and mutation databases

BioMutaiPRMT10
DMDMi74758248

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003259291 – 845Protein arginine N-methyltransferase 9Add BLAST845

Proteomic databases

EPDiQ6P2P2
MaxQBiQ6P2P2
PaxDbiQ6P2P2
PeptideAtlasiQ6P2P2
PRIDEiQ6P2P2
ProteomicsDBi66914
66915 [Q6P2P2-2]

PTM databases

iPTMnetiQ6P2P2
PhosphoSitePlusiQ6P2P2

Expressioni

Gene expression databases

BgeeiENSG00000164169 Expressed in 188 organ(s), highest expression level in right ovary
ExpressionAtlasiQ6P2P2 baseline and differential
GenevisibleiQ6P2P2 HS

Organism-specific databases

HPAiHPA036844
HPA042537

Interactioni

Subunit structurei

Found in a complex with PRMT9, SF3B2 and SF3B4 (PubMed:25737013). Interacts with SF3B2 (PubMed:25737013).1 Publication

Protein-protein interaction databases

BioGridi124766, 13 interactors
IntActiQ6P2P2, 4 interactors
STRINGi9606.ENSP00000314396

Structurei

3D structure databases

ProteinModelPortaliQ6P2P2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati25 – 58TPR 1Add BLAST34
Repeati67 – 100TPR 2Add BLAST34
Repeati101 – 134TPR 3Add BLAST34
Domaini137 – 466SAM-dependent MTase PRMT-type 1PROSITE-ProRule annotationAdd BLAST330
Domaini530 – 845SAM-dependent MTase PRMT-type 2PROSITE-ProRule annotationAdd BLAST316

Sequence similaritiesi

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiENOG410INRY Eukaryota
ENOG410Y35C LUCA
GeneTreeiENSGT00870000136479
HOGENOMiHOG000285961
HOVERGENiHBG104968
InParanoidiQ6P2P2
KOiK19737
OMAiVEKDQHR
OrthoDBiEOG091G0FS4
PhylomeDBiQ6P2P2
TreeFamiTF315221

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR025799 Arg_MeTrfase
IPR029063 SAM-dependent_MTases
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
SUPFAMiSSF48452 SSF48452, 1 hit
SSF53335 SSF53335, 2 hits
PROSITEiView protein in PROSITE
PS51678 SAM_MT_PRMT, 2 hits
PS50005 TPR, 2 hits
PS50293 TPR_REGION, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q6P2P2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSNSRPRSRR DAGGGAGAAG RDELVSRSLQ SAEHCLGVQD FGTAYAHYLL
60 70 80 90 100
VLSLAPELKH DVKETFQYTL FRWAEELDAL SRIQDLLGCY EQALELFPDD
110 120 130 140 150
EVICNSMGEH LFRMGFRDEA AGYFHKAVKL NPDFSDAKEN FYRVANWLVE
160 170 180 190 200
RWHFIMLNDT KRNTIYNAAI QKAVCLGSKS VLDIGAGTGI LSMFAKKAGA
210 220 230 240 250
HSVYACELSK TMYELACDVV AANKMEAGIK LLHTKSLDIE IPKHIPERVS
260 270 280 290 300
LVVTETVDAG LFGEGIVESL IHAWEHLLLQ PKTKGESANC EKYGKVIPAS
310 320 330 340 350
AVIFGMAVEC AEIRRHHRVG IKDIAGIHLP TNVKFQSPAY SSVDTEETIE
360 370 380 390 400
PYTTEKMSRV PGGYLALTEC FEIMTVDFNN LQELKSLATK KPDKIGIPVI
410 420 430 440 450
KEGILDAIMV WFVLQLDDEH SLSTSPSEET CWEQAVYPVQ DLADYWIKPG
460 470 480 490 500
DHVMMEVSCQ DCYLRIQSIS VLGLECEMDV AKSFTQNKDL LSLGNEAELC
510 520 530 540 550
SALANLQTSK PDAVEQTCIL ESTEIALLNN IPYHEGFKMA MSKVLSSLTP
560 570 580 590 600
EKLYQTMDTH CQNEMSSGTG QSNTVQNILE PFYVLDVSEG FSVLPVIAGT
610 620 630 640 650
LGQVKPYSSV EKDQHRIALD LISEANHFPK ETLEFWLRHV EDESAMLQRP
660 670 680 690 700
KSDKLWSIII LDVIEPSGLI QQEIMEKAAI SRCLLQSGGK IFPQYVLMFG
710 720 730 740 750
LLVESQTLLE ENAVQGTERT LGLNIAPFIN QFQVPIRVFL DLSSLPCIPL
760 770 780 790 800
SKPVELLRLD LMTPYLNTSN REVKVYVCKS GRLTAIPFWY HMYLDEEIRL
810 820 830 840
DTSSEASHWK QAAVVLDNPI QVEMGEELVL SIQHHKSNVS ITVKQ
Length:845
Mass (Da):94,501
Last modified:July 5, 2004 - v1
Checksum:i96C509E190D05A53
GO
Isoform 2 (identifier: Q6P2P2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-113: Missing.

Show »
Length:732
Mass (Da):81,857
Checksum:iEF627AD71327807F
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6REN0D6REN0_HUMAN
Protein arginine N-methyltransferas...
PRMT9
66Annotation score:

Sequence cautioni

The sequence BAC87459 differs from that shown. Intron retention.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti504A → P in AAH21250 (PubMed:15489334).Curated1
Sequence conflicti805E → G in BAF85593 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_039954483S → G. Corresponds to variant dbSNP:rs17023638Ensembl.1
Natural variantiVAR_039955747C → Y. Corresponds to variant dbSNP:rs11557361Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0539721 – 113Missing in isoform 2. 1 PublicationAdd BLAST113

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK128483 mRNA Translation: BAC87459.1 Sequence problems.
AK096703 mRNA Translation: BAG53354.1
AK292904 mRNA Translation: BAF85593.1
AC093835 Genomic DNA No translation available.
CH471056 Genomic DNA Translation: EAX05013.1
BC004337 mRNA Translation: AAH04337.1
BC021250 mRNA Translation: AAH21250.2
BC064403 mRNA Translation: AAH64403.1
AL137452 mRNA Translation: CAB70744.1
CH471056 Genomic DNA Translation: EAX05011.1
CCDSiCCDS3771.1 [Q6P2P2-1]
PIRiT46267
RefSeqiNP_001291387.1, NM_001304458.1 [Q6P2P2-2]
NP_612373.2, NM_138364.3 [Q6P2P2-1]
UniGeneiHs.591692

Genome annotation databases

EnsembliENST00000322396; ENSP00000314396; ENSG00000164169 [Q6P2P2-1]
GeneIDi90826
KEGGihsa:90826
UCSCiuc003ilc.4 human [Q6P2P2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK128483 mRNA Translation: BAC87459.1 Sequence problems.
AK096703 mRNA Translation: BAG53354.1
AK292904 mRNA Translation: BAF85593.1
AC093835 Genomic DNA No translation available.
CH471056 Genomic DNA Translation: EAX05013.1
BC004337 mRNA Translation: AAH04337.1
BC021250 mRNA Translation: AAH21250.2
BC064403 mRNA Translation: AAH64403.1
AL137452 mRNA Translation: CAB70744.1
CH471056 Genomic DNA Translation: EAX05011.1
CCDSiCCDS3771.1 [Q6P2P2-1]
PIRiT46267
RefSeqiNP_001291387.1, NM_001304458.1 [Q6P2P2-2]
NP_612373.2, NM_138364.3 [Q6P2P2-1]
UniGeneiHs.591692

3D structure databases

ProteinModelPortaliQ6P2P2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124766, 13 interactors
IntActiQ6P2P2, 4 interactors
STRINGi9606.ENSP00000314396

PTM databases

iPTMnetiQ6P2P2
PhosphoSitePlusiQ6P2P2

Polymorphism and mutation databases

BioMutaiPRMT10
DMDMi74758248

Proteomic databases

EPDiQ6P2P2
MaxQBiQ6P2P2
PaxDbiQ6P2P2
PeptideAtlasiQ6P2P2
PRIDEiQ6P2P2
ProteomicsDBi66914
66915 [Q6P2P2-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000322396; ENSP00000314396; ENSG00000164169 [Q6P2P2-1]
GeneIDi90826
KEGGihsa:90826
UCSCiuc003ilc.4 human [Q6P2P2-1]

Organism-specific databases

CTDi90826
EuPathDBiHostDB:ENSG00000164169.12
GeneCardsiPRMT9
HGNCiHGNC:25099 PRMT9
HPAiHPA036844
HPA042537
MIMi616125 gene
neXtProtiNX_Q6P2P2
OpenTargetsiENSG00000164169
PharmGKBiPA165664476
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410INRY Eukaryota
ENOG410Y35C LUCA
GeneTreeiENSGT00870000136479
HOGENOMiHOG000285961
HOVERGENiHBG104968
InParanoidiQ6P2P2
KOiK19737
OMAiVEKDQHR
OrthoDBiEOG091G0FS4
PhylomeDBiQ6P2P2
TreeFamiTF315221

Miscellaneous databases

ChiTaRSiPRMT9 human
GenomeRNAii90826
PROiPR:Q6P2P2
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000164169 Expressed in 188 organ(s), highest expression level in right ovary
ExpressionAtlasiQ6P2P2 baseline and differential
GenevisibleiQ6P2P2 HS

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR025799 Arg_MeTrfase
IPR029063 SAM-dependent_MTases
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
SUPFAMiSSF48452 SSF48452, 1 hit
SSF53335 SSF53335, 2 hits
PROSITEiView protein in PROSITE
PS51678 SAM_MT_PRMT, 2 hits
PS50005 TPR, 2 hits
PS50293 TPR_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiANM9_HUMAN
AccessioniPrimary (citable) accession number: Q6P2P2
Secondary accession number(s): A8KA39
, B3KU92, Q6ZR58, Q8N383, Q9BT55, Q9NT98
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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