UniProtKB - Q6P2P2 (ANM9_HUMAN)
Protein
Protein arginine N-methyltransferase 9
Gene
PRMT9
Organism
Homo sapiens (Human)
Status
Functioni
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA). Specifically mediates the symmetrical dimethylation of SF3B2. Involved in the regulation of alternative splicing of pre-mRNA (PubMed:25737013, PubMed:25979344).2 Publications
Caution
This protein should not be confused with FBXO11 (AC Q86XK2) that was initially erroneously named PRMT9.Curated
Catalytic activityi
- L-arginyl-[protein] + 2 S-adenosyl-L-methionine = 2 H+ + Nω,Nω'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine3 PublicationsEC:2.1.1.3203 Publications
pH dependencei
Optimum pH is between is between 7.5 and 8.0.1 Publication
Temperature dependencei
Optimum temperature is 37 degrees Celsius.1 Publication
GO - Molecular functioni
- protein-arginine N-methyltransferase activity Source: UniProtKB
- protein-arginine omega-N monomethyltransferase activity Source: GO_Central
- protein-arginine omega-N symmetric methyltransferase activity Source: UniProtKB
GO - Biological processi
- mRNA processing Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000164169-MONOMER |
PathwayCommonsi | Q6P2P2 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein arginine N-methyltransferase 9Alternative name(s): Protein arginine N-methyltransferase 10 (EC:2.1.1.3203 Publications) |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25099, PRMT9 |
MIMi | 616125, gene |
neXtProti | NX_Q6P2P2 |
VEuPathDBi | HostDB:ENSG00000164169.12 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 182 – 185 | LDIG → AAAA: Loss of interaction with SF3B2; Abolishes enzymatic activity. 1 Publication | 4 | |
Mutagenesisi | 258 | D → G: Abolishes enzymatic activity. 1 Publication | 1 | |
Mutagenesisi | 260 | G → E: Abolishes enzymatic activity. 1 Publication | 1 | |
Mutagenesisi | 431 | C → H: 8-fold increase in MMA production and almost complete elimination of sDMA production. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 90826 |
OpenTargetsi | ENSG00000164169 |
PharmGKBi | PA165664476 |
Miscellaneous databases
Pharosi | Q6P2P2, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4105724 |
Genetic variation databases
BioMutai | PRMT9 |
DMDMi | 74758248 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000325929 | 1 – 845 | Protein arginine N-methyltransferase 9Add BLAST | 845 |
Proteomic databases
EPDi | Q6P2P2 |
jPOSTi | Q6P2P2 |
MassIVEi | Q6P2P2 |
MaxQBi | Q6P2P2 |
PaxDbi | Q6P2P2 |
PeptideAtlasi | Q6P2P2 |
PRIDEi | Q6P2P2 |
ProteomicsDBi | 3704 66914 [Q6P2P2-1] 66915 [Q6P2P2-2] |
PTM databases
iPTMneti | Q6P2P2 |
PhosphoSitePlusi | Q6P2P2 |
Expressioni
Gene expression databases
Bgeei | ENSG00000164169, Expressed in right ovary and 202 other tissues |
ExpressionAtlasi | Q6P2P2, baseline and differential |
Genevisiblei | Q6P2P2, HS |
Organism-specific databases
HPAi | ENSG00000164169, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 124766, 15 interactors |
IntActi | Q6P2P2, 12 interactors |
STRINGi | 9606.ENSP00000314396 |
Miscellaneous databases
RNActi | Q6P2P2, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q6P2P2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 25 – 58 | TPR 1Add BLAST | 34 | |
Repeati | 67 – 100 | TPR 2Add BLAST | 34 | |
Repeati | 101 – 134 | TPR 3Add BLAST | 34 | |
Domaini | 137 – 466 | SAM-dependent MTase PRMT-type 1PROSITE-ProRule annotationAdd BLAST | 330 | |
Domaini | 530 – 845 | SAM-dependent MTase PRMT-type 2PROSITE-ProRule annotationAdd BLAST | 316 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.PROSITE-ProRule annotation
Keywords - Domaini
Repeat, TPR repeatPhylogenomic databases
eggNOGi | KOG1501, Eukaryota |
GeneTreei | ENSGT00940000158472 |
HOGENOMi | CLU_017482_1_0_1 |
InParanoidi | Q6P2P2 |
OMAi | HICKSGQ |
OrthoDBi | 519653at2759 |
PhylomeDBi | Q6P2P2 |
TreeFami | TF315221 |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR025799, Arg_MeTrfase IPR029063, SAM-dependent_MTases IPR013026, TPR-contain_dom IPR011990, TPR-like_helical_dom_sf IPR019734, TPR_repeat |
SUPFAMi | SSF48452, SSF48452, 1 hit SSF53335, SSF53335, 2 hits |
PROSITEi | View protein in PROSITE PS51678, SAM_MT_PRMT, 2 hits PS50005, TPR, 2 hits PS50293, TPR_REGION, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q6P2P2-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSNSRPRSRR DAGGGAGAAG RDELVSRSLQ SAEHCLGVQD FGTAYAHYLL
60 70 80 90 100
VLSLAPELKH DVKETFQYTL FRWAEELDAL SRIQDLLGCY EQALELFPDD
110 120 130 140 150
EVICNSMGEH LFRMGFRDEA AGYFHKAVKL NPDFSDAKEN FYRVANWLVE
160 170 180 190 200
RWHFIMLNDT KRNTIYNAAI QKAVCLGSKS VLDIGAGTGI LSMFAKKAGA
210 220 230 240 250
HSVYACELSK TMYELACDVV AANKMEAGIK LLHTKSLDIE IPKHIPERVS
260 270 280 290 300
LVVTETVDAG LFGEGIVESL IHAWEHLLLQ PKTKGESANC EKYGKVIPAS
310 320 330 340 350
AVIFGMAVEC AEIRRHHRVG IKDIAGIHLP TNVKFQSPAY SSVDTEETIE
360 370 380 390 400
PYTTEKMSRV PGGYLALTEC FEIMTVDFNN LQELKSLATK KPDKIGIPVI
410 420 430 440 450
KEGILDAIMV WFVLQLDDEH SLSTSPSEET CWEQAVYPVQ DLADYWIKPG
460 470 480 490 500
DHVMMEVSCQ DCYLRIQSIS VLGLECEMDV AKSFTQNKDL LSLGNEAELC
510 520 530 540 550
SALANLQTSK PDAVEQTCIL ESTEIALLNN IPYHEGFKMA MSKVLSSLTP
560 570 580 590 600
EKLYQTMDTH CQNEMSSGTG QSNTVQNILE PFYVLDVSEG FSVLPVIAGT
610 620 630 640 650
LGQVKPYSSV EKDQHRIALD LISEANHFPK ETLEFWLRHV EDESAMLQRP
660 670 680 690 700
KSDKLWSIII LDVIEPSGLI QQEIMEKAAI SRCLLQSGGK IFPQYVLMFG
710 720 730 740 750
LLVESQTLLE ENAVQGTERT LGLNIAPFIN QFQVPIRVFL DLSSLPCIPL
760 770 780 790 800
SKPVELLRLD LMTPYLNTSN REVKVYVCKS GRLTAIPFWY HMYLDEEIRL
810 820 830 840
DTSSEASHWK QAAVVLDNPI QVEMGEELVL SIQHHKSNVS ITVKQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD6REN0 | D6REN0_HUMAN | Protein arginine N-methyltransferas... | PRMT9 | 66 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 504 | A → P in AAH21250 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 805 | E → G in BAF85593 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_039954 | 483 | S → G. Corresponds to variant dbSNP:rs17023638Ensembl. | 1 | |
Natural variantiVAR_039955 | 747 | C → Y. Corresponds to variant dbSNP:rs11557361Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_053972 | 1 – 113 | Missing in isoform 2. 1 PublicationAdd BLAST | 113 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK128483 mRNA Translation: BAC87459.1 Sequence problems. AK096703 mRNA Translation: BAG53354.1 AK292904 mRNA Translation: BAF85593.1 AC093835 Genomic DNA No translation available. CH471056 Genomic DNA Translation: EAX05013.1 BC004337 mRNA Translation: AAH04337.1 BC021250 mRNA Translation: AAH21250.2 BC064403 mRNA Translation: AAH64403.1 AL137452 mRNA Translation: CAB70744.1 CH471056 Genomic DNA Translation: EAX05011.1 |
CCDSi | CCDS3771.1 [Q6P2P2-1] |
PIRi | T46267 |
RefSeqi | NP_001291387.1, NM_001304458.1 [Q6P2P2-2] NP_612373.2, NM_138364.3 [Q6P2P2-1] |
Genome annotation databases
Ensembli | ENST00000322396; ENSP00000314396; ENSG00000164169 [Q6P2P2-1] |
GeneIDi | 90826 |
KEGGi | hsa:90826 |
UCSCi | uc003ilc.4, human [Q6P2P2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK128483 mRNA Translation: BAC87459.1 Sequence problems. AK096703 mRNA Translation: BAG53354.1 AK292904 mRNA Translation: BAF85593.1 AC093835 Genomic DNA No translation available. CH471056 Genomic DNA Translation: EAX05013.1 BC004337 mRNA Translation: AAH04337.1 BC021250 mRNA Translation: AAH21250.2 BC064403 mRNA Translation: AAH64403.1 AL137452 mRNA Translation: CAB70744.1 CH471056 Genomic DNA Translation: EAX05011.1 |
CCDSi | CCDS3771.1 [Q6P2P2-1] |
PIRi | T46267 |
RefSeqi | NP_001291387.1, NM_001304458.1 [Q6P2P2-2] NP_612373.2, NM_138364.3 [Q6P2P2-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6PDM | X-ray | 2.45 | A | 127-845 | [»] | |
SMRi | Q6P2P2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 124766, 15 interactors |
IntActi | Q6P2P2, 12 interactors |
STRINGi | 9606.ENSP00000314396 |
Chemistry databases
ChEMBLi | CHEMBL4105724 |
PTM databases
iPTMneti | Q6P2P2 |
PhosphoSitePlusi | Q6P2P2 |
Genetic variation databases
BioMutai | PRMT9 |
DMDMi | 74758248 |
Proteomic databases
EPDi | Q6P2P2 |
jPOSTi | Q6P2P2 |
MassIVEi | Q6P2P2 |
MaxQBi | Q6P2P2 |
PaxDbi | Q6P2P2 |
PeptideAtlasi | Q6P2P2 |
PRIDEi | Q6P2P2 |
ProteomicsDBi | 3704 66914 [Q6P2P2-1] 66915 [Q6P2P2-2] |
Protocols and materials databases
Antibodypediai | 49190, 127 antibodies |
Genome annotation databases
Ensembli | ENST00000322396; ENSP00000314396; ENSG00000164169 [Q6P2P2-1] |
GeneIDi | 90826 |
KEGGi | hsa:90826 |
UCSCi | uc003ilc.4, human [Q6P2P2-1] |
Organism-specific databases
CTDi | 90826 |
DisGeNETi | 90826 |
GeneCardsi | PRMT9 |
HGNCi | HGNC:25099, PRMT9 |
HPAi | ENSG00000164169, Low tissue specificity |
MIMi | 616125, gene |
neXtProti | NX_Q6P2P2 |
OpenTargetsi | ENSG00000164169 |
PharmGKBi | PA165664476 |
VEuPathDBi | HostDB:ENSG00000164169.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1501, Eukaryota |
GeneTreei | ENSGT00940000158472 |
HOGENOMi | CLU_017482_1_0_1 |
InParanoidi | Q6P2P2 |
OMAi | HICKSGQ |
OrthoDBi | 519653at2759 |
PhylomeDBi | Q6P2P2 |
TreeFami | TF315221 |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000164169-MONOMER |
PathwayCommonsi | Q6P2P2 |
Miscellaneous databases
BioGRID-ORCSi | 90826, 13 hits in 993 CRISPR screens |
ChiTaRSi | PRMT9, human |
GenomeRNAii | 90826 |
Pharosi | Q6P2P2, Tbio |
PROi | PR:Q6P2P2 |
RNActi | Q6P2P2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000164169, Expressed in right ovary and 202 other tissues |
ExpressionAtlasi | Q6P2P2, baseline and differential |
Genevisiblei | Q6P2P2, HS |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR025799, Arg_MeTrfase IPR029063, SAM-dependent_MTases IPR013026, TPR-contain_dom IPR011990, TPR-like_helical_dom_sf IPR019734, TPR_repeat |
SUPFAMi | SSF48452, SSF48452, 1 hit SSF53335, SSF53335, 2 hits |
PROSITEi | View protein in PROSITE PS51678, SAM_MT_PRMT, 2 hits PS50005, TPR, 2 hits PS50293, TPR_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ANM9_HUMAN | |
Accessioni | Q6P2P2Primary (citable) accession number: Q6P2P2 Secondary accession number(s): A8KA39 Q9NT98 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | July 5, 2004 | |
Last modified: | April 7, 2021 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families