Nsd3

Functionⁱ

Histone methyltransferase. Preferentially dimethylates 'Lys-4' and 'Lys-27' of histone H3 forming H3K2me2 and H3K27me2. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.By similarity

Catalytic activityⁱ

L-lysyl²⁷-[histone H3]
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
+
S-adenosyl-L-methionine
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Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
=
H⁺
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Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
N⁶-methyl-L-lysyl²⁷-[histone H3]
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Search chemical reactions in Rhea for this molecule.
+
S-adenosyl-L-homocysteine
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Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
By similarity
Manual assertion inferred from sequence similarity toⁱ
- UniProtKB:Q9BZ95 (NSD3_HUMAN)
Source:
Rhea
Search for this reaction in UniProtKB.
See the description of this reaction in Rhea.
. Show »« Hide
L-lysyl²⁷-[histone H3]
L-lysine residue
zoom
+
S-adenosyl-L-methionine
zoom
=
H⁺
+
N⁶-methyl-L-lysyl²⁷-[histone H3]
N⁶-methyl-L-lysine residue
zoom
+
S-adenosyl-L-homocysteine
zoom
N⁶-methyl-L-lysyl²⁷-[histone H3]
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Search chemical reactions in Rhea for this molecule.
+
S-adenosyl-L-methionine
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Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
=
H⁺
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Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
N⁶,N⁶-dimethyl-L-lysyl²⁷-[histone H3]
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Search chemical reactions in Rhea for this molecule.
+
S-adenosyl-L-homocysteine
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Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
By similarity
Manual assertion inferred from sequence similarity toⁱ
- UniProtKB:Q9BZ95 (NSD3_HUMAN)
Source:
Rhea
Search for this reaction in UniProtKB.
See the description of this reaction in Rhea.
. Show »« Hide
N⁶-methyl-L-lysyl²⁷-[histone H3]
N⁶-methyl-L-lysine residue
zoom
+
S-adenosyl-L-methionine
zoom
=
H⁺
+
N⁶,N⁶-dimethyl-L-lysyl²⁷-[histone H3]
N⁶,N⁶-dimethyl-L-lysine residue
zoom
+
S-adenosyl-L-homocysteine
zoom
L-lysyl⁴-[histone H3]
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Search chemical reactions in Rhea for this molecule.
+
S-adenosyl-L-methionine
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Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
=
H⁺
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
N⁶-methyl-L-lysyl⁴-[histone H3]
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Search chemical reactions in Rhea for this molecule.
+
S-adenosyl-L-homocysteine
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
By similarity
Manual assertion inferred from sequence similarity toⁱ
- UniProtKB:Q9BZ95 (NSD3_HUMAN)
Source:
Rhea
Search for this reaction in UniProtKB.
See the description of this reaction in Rhea.
. Show »« Hide
L-lysyl⁴-[histone H3]
L-lysine residue
zoom
+
S-adenosyl-L-methionine
zoom
=
H⁺
+
N⁶-methyl-L-lysyl⁴-[histone H3]
N⁶-methyl-L-lysine residue
zoom
+
S-adenosyl-L-homocysteine
zoom
N⁶-methyl-L-lysyl⁴-[histone H3]
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
+
S-adenosyl-L-methionine
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
=
H⁺
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
N⁶,N⁶-dimethyl-L-lysyl⁴-[histone H3]
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
+
S-adenosyl-L-homocysteine
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
By similarity
Manual assertion inferred from sequence similarity toⁱ
- UniProtKB:Q9BZ95 (NSD3_HUMAN)
Source:
Rhea
Search for this reaction in UniProtKB.
See the description of this reaction in Rhea.
. Show »« Hide
N⁶-methyl-L-lysyl⁴-[histone H3]
N⁶-methyl-L-lysine residue
zoom
+
S-adenosyl-L-methionine
zoom
=
H⁺
+
N⁶,N⁶-dimethyl-L-lysyl⁴-[histone H3]
N⁶,N⁶-dimethyl-L-lysine residue
zoom
+
S-adenosyl-L-homocysteine
zoom

Regions

Feature key	Position(s)	DescriptionActions	Length
Zinc fingerⁱ	701 – 748	PHD-type 1PROSITE-ProRule annotationAdd BLAST	48
Zinc fingerⁱ	749 – 805	PHD-type 2PROSITE-ProRule annotationAdd BLAST	57
Zinc fingerⁱ	862 – 955	PHD-type 3PROSITE-ProRule annotationAdd BLAST	94
Zinc fingerⁱ	1323 – 1370	PHD-type 4; atypicalPROSITE-ProRule annotationAdd BLAST	48

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Chromatin regulator, Methyltransferase, Transferase
Biological process	Transcription, Transcription regulation
Ligand	Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-MMU-3214841, PKMTs methylate histone lysines

Reactomeⁱ

R-MMU-3214841, PKMTs methylate histone lysines

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Histone-lysine N-methyltransferase NSD3 (EC:2.1.1.-By similarity) Alternative name(s): Nuclear SET domain-containing protein 3 Wolf-Hirschhorn syndrome candidate 1-like protein 1 homolog Short name: WHSC1-like protein 1
Gene namesⁱ	Name:Nsd3 Synonyms:Whsc1l1
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 8

Organism-specific databases

MGIⁱ	MGI:2142581, Nsd3

Keywords - Cellular componentⁱ

Chromosome, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000259522	1 – 1439	Histone-lysine N-methyltransferase NSD3Add BLAST		1439

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	150	PhosphoserineBy similarity	1
Cross-linkⁱ	218	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	245	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	413	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	457	PhosphoserineBy similarity	1
Cross-linkⁱ	502	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	532	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	585	PhosphoserineBy similarity	1
Modified residueⁱ	587	PhosphoserineBy similarity	1
Modified residueⁱ	590	PhosphoserineBy similarity	1
Cross-linkⁱ	628	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	655	PhosphoserineBy similarity	1
Modified residueⁱ	790	N6-acetyllysineCombined sources	1
Cross-linkⁱ	1154	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMⁱ

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

jPOSTⁱ	Q6P2L6
PaxDbⁱ	Q6P2L6
PeptideAtlas More...PeptideAtlasⁱ	Q6P2L6
PRIDEⁱ	Q6P2L6

PTM databases

iPTMnetⁱ	Q6P2L6
PhosphoSitePlusⁱ	Q6P2L6

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000054823, Expressed in bone marrow and 267 other tissues
ExpressionAtlasⁱ	Q6P2L6, baseline and differential
Genevisibleⁱ	Q6P2L6, MM

Interactionⁱ

Subunit structureⁱ

Interacts with BRD4.

By similarity

Protein-protein interaction databases

BioGRIDⁱ	231497, 1 interactor
IntActⁱ	Q6P2L6, 2 interactors
Molecular INTeraction database More...MINTⁱ	Q6P2L6
STRINGⁱ	10090.ENSMUSP00000115470

Miscellaneous databases

RNActⁱ	Q6P2L6, protein

RNActⁱ

Q6P2L6, protein

Structureⁱ

3D structure databases

SMRⁱ	Q6P2L6
ModBaseⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	270 – 333	PWWP 1PROSITE-ProRule annotationAdd BLAST	64
Domainⁱ	960 – 1025	PWWP 2PROSITE-ProRule annotationAdd BLAST	66
Domainⁱ	1096 – 1146	AWSPROSITE-ProRule annotationAdd BLAST	51
Domainⁱ	1148 – 1265	SETPROSITE-ProRule annotationAdd BLAST	118
Domainⁱ	1272 – 1288	Post-SETPROSITE-ProRule annotationAdd BLAST	17

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	1036 – 1065	Sequence analysisAdd BLAST		30

Sequence similaritiesⁱ

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.PROSITE-ProRule annotation

Zinc finger

Feature key	Position(s)	DescriptionActions	Length
Zinc fingerⁱ	701 – 748	PHD-type 1PROSITE-ProRule annotationAdd BLAST	48
Zinc fingerⁱ	749 – 805	PHD-type 2PROSITE-ProRule annotationAdd BLAST	57
Zinc fingerⁱ	862 – 955	PHD-type 3PROSITE-ProRule annotationAdd BLAST	94
Zinc fingerⁱ	1323 – 1370	PHD-type 4; atypicalPROSITE-ProRule annotationAdd BLAST	48

Keywords - Domainⁱ

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG1081, Eukaryota
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00940000155355
HOGENOMⁱ	CLU_488831_0_0_1
InParanoidⁱ	Q6P2L6
KEGG Orthology (KO) More...KOⁱ	K11425

Family and domain databases

Gene3Dⁱ	3.30.40.10, 4 hits
InterProⁱ	View protein in InterPro IPR006560, AWS_dom IPR041306, C5HCH IPR003616, Post-SET_dom IPR000313, PWWP_dom IPR001214, SET_dom IPR019786, Zinc_finger_PHD-type_CS IPR011011, Znf_FYVE_PHD IPR001965, Znf_PHD IPR019787, Znf_PHD-finger IPR013083, Znf_RING/FYVE/PHD
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF17907, AWS, 1 hit PF17982, C5HCH, 1 hit PF00855, PWWP, 2 hits PF00856, SET, 1 hit
SMARTⁱ	View protein in SMART SM00570, AWS, 1 hit SM00249, PHD, 5 hits SM00508, PostSET, 1 hit SM00293, PWWP, 2 hits SM00317, SET, 1 hit
SUPFAMⁱ	SSF57903, SSF57903, 3 hits
PROSITEⁱ	View protein in PROSITE PS51215, AWS, 1 hit PS50868, POST_SET, 1 hit PS50812, PWWP, 2 hits PS50280, SET, 1 hit PS01359, ZF_PHD_1, 1 hit PS50016, ZF_PHD_2, 2 hits

Sequences (4+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 4 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q6P2L6-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the canonicalⁱ sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MDFSFSFMQG IMGNTIQQPP QLIDSANIRQ EDAFDNHSDI VEDGGPTPFE 
        60         70         80         90        100
ATLQQGFQYP PTTEDLPPLT NGYPPSISLY ETQTKYPPYN QYPNGSANGF 
       110        120        130        140        150
GAVRNFSPTD YYHSEIPNTR PHEILEKPSP PQPPPPPSVP QTVIPKKTGS 
       160        170        180        190        200
PEIKLKITKT IQNGRELFES SLCGDLLNEV QASEHTKSKH ESRKEKRKKS 
       210        220        230        240        250
NRHESSRSEE RRSHKIPKLE PEGQNRPNER VDTAPEKPRE EPVLKEAIPV 
       260        270        280        290        300
QPILSSVPTT ETSTGVKFQV GDLVWSKVGT YPWWPCMVSS DPQLEVHSKI 
       310        320        330        340        350
NTRGAREYHV QFFSNQPERA WVHEKRVREY KGHEQYEELL AEAAKQASNH 
       360        370        380        390        400
SEKQKIRKPR PQRERAQWDI GIAHAEKALK MTREERVEQY TFIYIDKQPE 
       410        420        430        440        450
EASSQAKKNV TSKTEVKKPR RPRSVLNSQP EQTNAGEVAS SQSSTDLRRQ 
       460        470        480        490        500
SQRRHTSLEE EEPPPVKIAW KTAAARKSLP ASITMHKGSL DLQKCNMSPV 
       510        520        530        540        550
VKIEQVFALQ NATGDGKFID QFVYSTKGIG NKTEISVRGQ DRLIISSPSQ 
       560        570        580        590        600
RSEKPAQSAS SPEATSGSAG PVEKKQQRRS IRTRSESEKS AEVVPKKKIK 
       610        620        630        640        650
KEQVETAPQA SLKTGLQKGA SEISDSCKPL KKRSRASTDV ETASCTYRDT 
       660        670        680        690        700
SDSDSRGLSD GQVGFGKQVD SPSATADADA SDAQSVDSSL SRRGVGTSKK 
       710        720        730        740        750
DTVCQVCEKA GDCLVACEGE CCRHFHVECL GLTAVPEGHF TCEECETGQH 
       760        770        780        790        800
PCFSCKVSGK DVKRCSVSVC GKFYHEACVR KFPTAIFESK GFRCPQHCCS 
       810        820        830        840        850
SCSMEKDIHK ASKGRMMRCL RCPVAYHVGD ACVAAGSVSV SSHILICSNH 
       860        870        880        890        900
SKRSSQSAAI NVGFCFVCAR GLIVQDHSDP MFSSYAYKSH YLLSESNRAE 
       910        920        930        940        950
LMKLPMIPSS SASKKRCEKG GRLLCCESCP ASFHPECLSI DMPEGCWNCN 
       960        970        980        990       1000
DCKAGKKLHY KQIVWVKLGN YRQVLWWPAE ICSPRSVPLN IQGLKHDLGD 
      1010       1020       1030       1040       1050
FPVFFFGSHD YYWVHQGRVF PYVEGDKHFA EGQTSINKTF KKALEEAAKR 
      1060       1070       1080       1090       1100
FQELKAQRES KEALEMERTS RKPPPYKHIK ANKVIGKVQV QVADLSEIPR 
      1110       1120       1130       1140       1150
CNCKPGDENP CGLESQCLNR MSQYECHPQV CPAGDRCQNQ CFTKRLYPDA 
      1160       1170       1180       1190       1200
EVIKTERRGW GLRTKRSIKK GEFVNEYVGE LIDEEECRLR IKRAHENSVT 
      1210       1220       1230       1240       1250
NFYMLTVTKD RIIDAGPKGN YSRFMNHSCN PNCETQKWTV NGDVRVGLFA 
      1260       1270       1280       1290       1300
LCDIPAGMEL TFNYNLDCLG NGRTVCHCGA DNCSGFLGVR PKSACTSAVD 
      1310       1320       1330       1340       1350
EKTKNAKLKK RRKVKAEAKP IHEDYCFQCG DGGELVMCDK KDCPKAYHLL 
      1360       1370       1380       1390       1400
CLNLTQPPHG KWECPWHRCD ECGSVAVSFC EFCPHSFCKA HGKGALVPSA 
      1410       1420       1430 
LEGRLCCSSH DPASPVSPEY WSKIRCKWES QDSGEEVKE

Length:1,439

Mass (Da):161,002

Last modified:October 31, 2006 - v2

Checksum:ⁱ917C7ADCD1248525

GO

Isoform 2 (identifier: Q6P2L6-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
     135-135: P → PLPPPPPPPPP
     620-645: ASEISDSCKPLKKRSRASTDVETASC → SADRGAQGSVRFSDSSVSAAKEETVD
     646-1439: Missing.

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        10         20         30         40         50
MDFSFSFMQG IMGNTIQQPP QLIDSANIRQ EDAFDNHSDI VEDGGPTPFE 
        60         70         80         90        100
ATLQQGFQYP PTTEDLPPLT NGYPPSISLY ETQTKYPPYN QYPNGSANGF 
       110        120        130        140        150
GAVRNFSPTD YYHSEIPNTR PHEILEKPSP PQPPPLPPPP PPPPPPPSVP 
       160        170        180        190        200
QTVIPKKTGS PEIKLKITKT IQNGRELFES SLCGDLLNEV QASEHTKSKH 
       210        220        230        240        250
ESRKEKRKKS NRHESSRSEE RRSHKIPKLE PEGQNRPNER VDTAPEKPRE 
       260        270        280        290        300
EPVLKEAIPV QPILSSVPTT ETSTGVKFQV GDLVWSKVGT YPWWPCMVSS 
       310        320        330        340        350
DPQLEVHSKI NTRGAREYHV QFFSNQPERA WVHEKRVREY KGHEQYEELL 
       360        370        380        390        400
AEAAKQASNH SEKQKIRKPR PQRERAQWDI GIAHAEKALK MTREERVEQY 
       410        420        430        440        450
TFIYIDKQPE EASSQAKKNV TSKTEVKKPR RPRSVLNSQP EQTNAGEVAS 
       460        470        480        490        500
SQSSTDLRRQ SQRRHTSLEE EEPPPVKIAW KTAAARKSLP ASITMHKGSL 
       510        520        530        540        550
DLQKCNMSPV VKIEQVFALQ NATGDGKFID QFVYSTKGIG NKTEISVRGQ 
       560        570        580        590        600
DRLIISSPSQ RSEKPAQSAS SPEATSGSAG PVEKKQQRRS IRTRSESEKS 
       610        620        630        640        650
AEVVPKKKIK KEQVETAPQA SLKTGLQKGS ADRGAQGSVR FSDSSVSAAK 

EETVD

Show »

Length:655

Mass (Da):73,380

Checksum:ⁱ358CBD840A5A47C6

GO

Isoform 3 (identifier: Q6P2L6-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-941: Missing.
     973-975: Missing.
     1199-1209: Missing.

« Hide

        10         20         30         40         50
MPEGCWNCND CKAGKKLHYK QIVWVKLGNY RWWPAEICSP RSVPLNIQGL 
        60         70         80         90        100
KHDLGDFPVF FFGSHDYYWV HQGRVFPYVE GDKHFAEGQT SINKTFKKAL 
       110        120        130        140        150
EEAAKRFQEL KAQRESKEAL EMERTSRKPP PYKHIKANKV IGKVQVQVAD 
       160        170        180        190        200
LSEIPRCNCK PGDENPCGLE SQCLNRMSQY ECHPQVCPAG DRCQNQCFTK 
       210        220        230        240        250
RLYPDAEVIK TERRGWGLRT KRSIKKGEFV NEYVGELIDE EECRLRIKRA 
       260        270        280        290        300
HENSDRIIDA GPKGNYSRFM NHSCNPNCET QKWTVNGDVR VGLFALCDIP 
       310        320        330        340        350
AGMELTFNYN LDCLGNGRTV CHCGADNCSG FLGVRPKSAC TSAVDEKTKN 
       360        370        380        390        400
AKLKKRRKVK AEAKPIHEDY CFQCGDGGEL VMCDKKDCPK AYHLLCLNLT 
       410        420        430        440        450
QPPHGKWECP WHRCDECGSV AVSFCEFCPH SFCKAHGKGA LVPSALEGRL 
       460        470        480 
CCSSHDPASP VSPEYWSKIR CKWESQDSGE EVKE

Show »

Length:484

Mass (Da):54,977

Checksum:ⁱ562572C7B3100FD4

GO

Isoform 4 (identifier: Q6P2L6-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
     135-135: P → PLPPPPPPPPP
     603-611: QVETAPQAS → QVGFLHVES
     612-1439: Missing.

« Hide

        10         20         30         40         50
MDFSFSFMQG IMGNTIQQPP QLIDSANIRQ EDAFDNHSDI VEDGGPTPFE 
        60         70         80         90        100
ATLQQGFQYP PTTEDLPPLT NGYPPSISLY ETQTKYPPYN QYPNGSANGF 
       110        120        130        140        150
GAVRNFSPTD YYHSEIPNTR PHEILEKPSP PQPPPLPPPP PPPPPPPSVP 
       160        170        180        190        200
QTVIPKKTGS PEIKLKITKT IQNGRELFES SLCGDLLNEV QASEHTKSKH 
       210        220        230        240        250
ESRKEKRKKS NRHESSRSEE RRSHKIPKLE PEGQNRPNER VDTAPEKPRE 
       260        270        280        290        300
EPVLKEAIPV QPILSSVPTT ETSTGVKFQV GDLVWSKVGT YPWWPCMVSS 
       310        320        330        340        350
DPQLEVHSKI NTRGAREYHV QFFSNQPERA WVHEKRVREY KGHEQYEELL 
       360        370        380        390        400
AEAAKQASNH SEKQKIRKPR PQRERAQWDI GIAHAEKALK MTREERVEQY 
       410        420        430        440        450
TFIYIDKQPE EASSQAKKNV TSKTEVKKPR RPRSVLNSQP EQTNAGEVAS 
       460        470        480        490        500
SQSSTDLRRQ SQRRHTSLEE EEPPPVKIAW KTAAARKSLP ASITMHKGSL 
       510        520        530        540        550
DLQKCNMSPV VKIEQVFALQ NATGDGKFID QFVYSTKGIG NKTEISVRGQ 
       560        570        580        590        600
DRLIISSPSQ RSEKPAQSAS SPEATSGSAG PVEKKQQRRS IRTRSESEKS 
       610        620 
AEVVPKKKIK KEQVGFLHVE S

Show »

Length:621

Mass (Da):70,000

Checksum:ⁱ5553A83F48BF9B16

GO

Computationally mapped potential isoform sequencesⁱ

There are 3 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

D3Z357	D3Z357_MOUSE	Histone-lysine N-methyltransferase ... Histone-lysine N-methyltransferase NSD3	Nsd3 Whsc1l1	1,446	Annotation score:
D3Z197	D3Z197_MOUSE	Histone-lysine N-methyltransferase ... Histone-lysine N-methyltransferase NSD3	Nsd3 Whsc1l1	1,397	Annotation score:
A0A1B0GSG3	A0A1B0GSG3_MOUSE	Histone-lysine N-methyltransferase ... Histone-lysine N-methyltransferase NSD3	Nsd3 Whsc1l1	542	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	122	H → R in BAE33834 (PubMed:16141072).Curated	1
Sequence conflictⁱ	950	N → K in BAE21322 (PubMed:16141072).Curated	1
Sequence conflictⁱ	1149	D → N in BAE21322 (PubMed:16141072).Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_021431	1 – 941	Missing in isoform 3. 1 PublicationAdd BLAST	941
Alternative sequenceⁱVSP_021432	135	P → PLPPPPPPPPP in isoform 2 and isoform 4. 2 Publications	1
Alternative sequenceⁱVSP_021433	603 – 611	QVETAPQAS → QVGFLHVES in isoform 4. 1 Publication	9
Alternative sequenceⁱVSP_021434	612 – 1439	Missing in isoform 4. 1 PublicationAdd BLAST	828
Alternative sequenceⁱVSP_021435	620 – 645	ASEIS…ETASC → SADRGAQGSVRFSDSSVSAA KEETVD in isoform 2. 2 PublicationsAdd BLAST	26
Alternative sequenceⁱVSP_021436	646 – 1439	Missing in isoform 2. 2 PublicationsAdd BLAST	794
Alternative sequenceⁱVSP_021437	973 – 975	Missing in isoform 3. 1 Publication	3
Alternative sequenceⁱVSP_021438	1199 – 1209	Missing in isoform 3. 1 PublicationAdd BLAST	11

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK079952 mRNA Translation: BAC37792.1 AK132725 mRNA Translation: BAE21322.1 AK156746 mRNA Translation: BAE33834.1 AK170040 mRNA Translation: BAE41526.1 AC156990 Genomic DNA No translation available. AC162367 Genomic DNA No translation available. BC064447 mRNA Translation: AAH64447.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS40305.1 [Q6P2L6-2] CCDS85518.1 [Q6P2L6-4]
NCBI Reference Sequences More...RefSeqⁱ	NP_001001735.1, NM_001001735.2 [Q6P2L6-2] NP_001295410.1, NM_001308481.1 [Q6P2L6-2] NP_001295411.1, NM_001308482.1 [Q6P2L6-4]

Genome annotation databases

Ensemblⁱ	ENSMUST00000136107; ENSMUSP00000147840; ENSMUSG00000054823 [Q6P2L6-4] ENSMUST00000146919; ENSMUSP00000115470; ENSMUSG00000054823 [Q6P2L6-2] ENSMUST00000153597; ENSMUSP00000123028; ENSMUSG00000054823 [Q6P2L6-3] ENSMUST00000155861; ENSMUSP00000117596; ENSMUSG00000054823 [Q6P2L6-2]
GeneIDⁱ	234135
KEGGⁱ	mmu:234135
UCSC genome browser More...UCSCⁱ	uc009lgk.1, mouse [Q6P2L6-4] uc009lgm.1, mouse [Q6P2L6-2] uc009lgp.1, mouse [Q6P2L6-3]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q6P2L6	Nuclear receptor-binding SET domain protein 3		RAT		1443	UniRef90_Q6P2L6
histone-lysine N-methyltransferase NSD3 isoform X1		PERMB		1438
histone-lysine N-methyltransferase NSD3 isoform X2		MESAU		1425
Histone-lysine N-methyltransferase NSD3		MOUSE		1397
histone-lysine N-methyltransferase NSD3 isoform X2		PERMB		1427
+26
Q6P2L6-2	Isoform 3 of Histone-lysine N-methyltransferase NSD3		HUMAN		645	UniRef90_Q9BZ95-3
PWWP domain-containing protein		MARMO		648
PWWP domain-containing protein		CAPHI		646
PWWP domain-containing protein		ICTTR		645
Histone-lysine N-methyltransferase NSD3		CASCN		645
+87
Q6P2L6-4	LOW QUALITY PROTEIN: histone-lysine N-methyltransferase NSD3-like		PERMB		715	UniRef90_Q6P2L6-4
histone-lysine N-methyltransferase NSD3 isoform X4		PERMB		647
histone-lysine N-methyltransferase NSD3 isoform X5		PERMB		606
+9

Protein	Similar proteins		Species	Score	Length	Source
Q6P2L6	Nuclear receptor-binding SET domain protein 3		RAT		1443	UniRef50_Q6P2L6
histone-lysine N-methyltransferase NSD3 isoform X1		PERMB		1438
histone-lysine N-methyltransferase NSD3 isoform X2		MESAU		1425
Histone-lysine N-methyltransferase NSD3		MOUSE		1397
histone-lysine N-methyltransferase NSD3 isoform X6		ACIJB		1381
+649
Q6P2L6-2	Isoform 3 of Histone-lysine N-methyltransferase NSD3		HUMAN		645	UniRef50_Q9BZ95-3
PWWP domain-containing protein		MARMO		648
PWWP domain-containing protein		CAPHI		646
histone-lysine N-methyltransferase NSD3 isoform X4		Enhydra lutris kenyoni		645
histone-lysine N-methyltransferase NSD3 isoform X4		LEPWE		645
+96
Q6P2L6-3	Nuclear receptor binding SET domain protein 1b		ASTMX		602	UniRef50_Q6P2L6-3
Uncharacterized protein		LITCT		595
Histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific		MYOBR		309
+2
Q6P2L6-4	LOW QUALITY PROTEIN: histone-lysine N-methyltransferase NSD3-like		PERMB		715	UniRef50_Q6P2L6-4
PWWP domain-containing protein		XENTR		582
PWWP domain-containing protein		MYOLU		643
PWWP domain-containing protein		BOBOX		646
histone-lysine N-methyltransferase NSD3 isoform X4		Odocoileus virginianus texanus		646
+58

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK079952 mRNA Translation: BAC37792.1 AK132725 mRNA Translation: BAE21322.1 AK156746 mRNA Translation: BAE33834.1 AK170040 mRNA Translation: BAE41526.1 AC156990 Genomic DNA No translation available. AC162367 Genomic DNA No translation available. BC064447 mRNA Translation: AAH64447.1
CCDSⁱ	CCDS40305.1 [Q6P2L6-2] CCDS85518.1 [Q6P2L6-4]
RefSeqⁱ	NP_001001735.1, NM_001001735.2 [Q6P2L6-2] NP_001295410.1, NM_001308481.1 [Q6P2L6-2] NP_001295411.1, NM_001308482.1 [Q6P2L6-4]

3D structure databases

SMRⁱ	Q6P2L6
ModBaseⁱ	Search...

Protein-protein interaction databases

BioGRIDⁱ	231497, 1 interactor
IntActⁱ	Q6P2L6, 2 interactors
MINTⁱ	Q6P2L6
STRINGⁱ	10090.ENSMUSP00000115470

PTM databases

iPTMnetⁱ	Q6P2L6
PhosphoSitePlusⁱ	Q6P2L6

Proteomic databases

jPOSTⁱ	Q6P2L6
PaxDbⁱ	Q6P2L6
PeptideAtlasⁱ	Q6P2L6
PRIDEⁱ	Q6P2L6

Protocols and materials databases

ABCDⁱ	Q6P2L6, 1 sequenced antibody
Antibodypediaⁱ	984, 334 antibodies
The DNASU plasmid repository More...DNASUⁱ	234135

Genome annotation databases

Ensemblⁱ	ENSMUST00000136107; ENSMUSP00000147840; ENSMUSG00000054823 [Q6P2L6-4] ENSMUST00000146919; ENSMUSP00000115470; ENSMUSG00000054823 [Q6P2L6-2] ENSMUST00000153597; ENSMUSP00000123028; ENSMUSG00000054823 [Q6P2L6-3] ENSMUST00000155861; ENSMUSP00000117596; ENSMUSG00000054823 [Q6P2L6-2]
GeneIDⁱ	234135
KEGGⁱ	mmu:234135
UCSCⁱ	uc009lgk.1, mouse [Q6P2L6-4] uc009lgm.1, mouse [Q6P2L6-2] uc009lgp.1, mouse [Q6P2L6-3]

Organism-specific databases

CTDⁱ	54904
MGIⁱ	MGI:2142581, Nsd3

Phylogenomic databases

eggNOGⁱ	KOG1081, Eukaryota
GeneTreeⁱ	ENSGT00940000155355
HOGENOMⁱ	CLU_488831_0_0_1
InParanoidⁱ	Q6P2L6
KOⁱ	K11425

Enzyme and pathway databases

Reactomeⁱ	R-MMU-3214841, PKMTs methylate histone lysines

Reactomeⁱ

R-MMU-3214841, PKMTs methylate histone lysines

Miscellaneous databases

BioGRID-ORCSⁱ	234135, 5 hits in 22 CRISPR screens
ChiTaRSⁱ	Whsc1l1, mouse
Protein Ontology More...PROⁱ	PR:Q6P2L6
RNActⁱ	Q6P2L6, protein
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000054823, Expressed in bone marrow and 267 other tissues
ExpressionAtlasⁱ	Q6P2L6, baseline and differential
Genevisibleⁱ	Q6P2L6, MM

Family and domain databases

Gene3Dⁱ	3.30.40.10, 4 hits
InterProⁱ	View protein in InterPro IPR006560, AWS_dom IPR041306, C5HCH IPR003616, Post-SET_dom IPR000313, PWWP_dom IPR001214, SET_dom IPR019786, Zinc_finger_PHD-type_CS IPR011011, Znf_FYVE_PHD IPR001965, Znf_PHD IPR019787, Znf_PHD-finger IPR013083, Znf_RING/FYVE/PHD
Pfamⁱ	View protein in Pfam PF17907, AWS, 1 hit PF17982, C5HCH, 1 hit PF00855, PWWP, 2 hits PF00856, SET, 1 hit
SMARTⁱ	View protein in SMART SM00570, AWS, 1 hit SM00249, PHD, 5 hits SM00508, PostSET, 1 hit SM00293, PWWP, 2 hits SM00317, SET, 1 hit
SUPFAMⁱ	SSF57903, SSF57903, 3 hits
PROSITEⁱ	View protein in PROSITE PS51215, AWS, 1 hit PS50868, POST_SET, 1 hit PS50812, PWWP, 2 hits PS50280, SET, 1 hit PS01359, ZF_PHD_1, 1 hit PS50016, ZF_PHD_2, 2 hits
ProtoNetⁱ	Search...
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NSD3_MOUSE
Accessionⁱ	Q6P2L6Primary (citable) accession number: Q6P2L6 Secondary accession number(s): Q3TDS4 , Q3U0L8, Q3V131, Q8BJT3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
	Last sequence update:	October 31, 2006
	Last modified:	October 7, 2020
	This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Direct protein sequencing, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
SIMILARITY comments
Index of protein domains and families

UniProtKB

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Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q6P2L6 (NSD3_MOUSE)

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Histone-lysine N-methyltransferase NSD3

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Coiled coil

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Catalytic activityⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ