UniProtKB - Q6P1B1 (XPP1_MOUSE)
Protein
Xaa-Pro aminopeptidase 1
Gene
Xpnpep1
Organism
Mus musculus (Mouse)
Status
Functioni
Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro (By similarity).By similarity
Catalytic activityi
- Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.By similarity EC:3.4.11.9
Cofactori
Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 77 | SubstrateBy similarity | 1 | |
Binding sitei | 395 | Substrate; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 415 | Manganese 1By similarity | 1 | |
Metal bindingi | 426 | Manganese 1By similarity | 1 | |
Metal bindingi | 426 | Manganese 2By similarity | 1 | |
Metal bindingi | 489 | Manganese 2; via tele nitrogenBy similarity | 1 | |
Binding sitei | 489 | Substrate; via tele nitrogenBy similarity | 1 | |
Binding sitei | 498 | Substrate; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 523 | Manganese 2By similarity | 1 | |
Binding sitei | 523 | SubstrateBy similarity | 1 | |
Metal bindingi | 537 | Manganese 1By similarity | 1 | |
Metal bindingi | 537 | Manganese 2By similarity | 1 |
GO - Molecular functioni
- aminopeptidase activity Source: UniProtKB
- manganese ion binding Source: UniProtKB
- metalloaminopeptidase activity Source: UniProtKB
- protein homodimerization activity Source: MGI
GO - Biological processi
- bradykinin catabolic process Source: UniProtKB
- proteolysis Source: UniProtKB
Keywordsi
Molecular function | Aminopeptidase, Hydrolase, Metalloprotease, Protease |
Ligand | Manganese, Metal-binding |
Protein family/group databases
MEROPSi | M24.009 |
Names & Taxonomyi
Protein namesi | Recommended name: Xaa-Pro aminopeptidase 1 (EC:3.4.11.9)Alternative name(s): Aminoacylproline aminopeptidase Cytosolic aminopeptidase P Soluble aminopeptidase P Short name: sAmp X-Pro aminopeptidase 1 X-prolyl aminopeptidase 1, soluble |
Gene namesi | Name:Xpnpep1Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2180003, Xpnpep1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000185084 | 1 – 623 | Xaa-Pro aminopeptidase 1Add BLAST | 623 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 304 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q6P1B1 |
jPOSTi | Q6P1B1 |
MaxQBi | Q6P1B1 |
PaxDbi | Q6P1B1 |
PRIDEi | Q6P1B1 |
PTM databases
iPTMneti | Q6P1B1 |
PhosphoSitePlusi | Q6P1B1 |
SwissPalmi | Q6P1B1 |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 228414, 14 interactors |
IntActi | Q6P1B1, 4 interactors |
MINTi | Q6P1B1 |
STRINGi | 10090.ENSMUSP00000138250 |
Miscellaneous databases
RNActi | Q6P1B1, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase M24B family.Sequence analysis
Phylogenomic databases
eggNOGi | KOG2413, Eukaryota |
GeneTreei | ENSGT00940000157716 |
InParanoidi | Q6P1B1 |
OrthoDBi | 417805at2759 |
PhylomeDBi | Q6P1B1 |
TreeFami | TF314183 |
Family and domain databases
CDDi | cd01085, APP, 1 hit |
Gene3Di | 3.40.350.10, 2 hits 3.90.230.10, 1 hit |
InterProi | View protein in InterPro IPR029149, Creatin/AminoP/Spt16_NTD IPR036005, Creatinase/aminopeptidase-like IPR000587, Creatinase_N IPR000994, Pept_M24 IPR033740, Pept_M24B IPR032416, Peptidase_M24_C IPR001131, Peptidase_M24B_aminopep-P_CS |
Pfami | View protein in Pfam PF01321, Creatinase_N, 1 hit PF00557, Peptidase_M24, 1 hit PF16188, Peptidase_M24_C, 1 hit |
SUPFAMi | SSF53092, SSF53092, 1 hit SSF55920, SSF55920, 1 hit |
PROSITEi | View protein in PROSITE PS00491, PROLINE_PEPTIDASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
Q6P1B1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPKVTSELL RQLRQAMRNS EYVAEPIQAY IIPSGDAHQS EYIAPCDCRR
60 70 80 90 100
AFVSGFDGSA GTAIITEEHA AMWTDGRYFL QAAKQMDNNW TLMKMGLKDT
110 120 130 140 150
PTQEDWLVSV LPEGSRVGVD PLIIPTDYWK KMAKVLRSAG HHLVPVKENL
160 170 180 190 200
VDKIWTDRPE RPCKPLLTLG LDYTGISWKE KVADLRLKMA ERSIAWFVVT
210 220 230 240 250
ALDEIAWLFN LRGSDVEHNP VFFSYAIVGL ETIMLFIDGD RVDAPGVKQH
260 270 280 290 300
LLLDLGLEAE YRIQVLPYKS ILSELKALCA DLSPREKVWV SDKASYAVSE
310 320 330 340 350
AIPKDHRCCM PYTPICIAKA VKNSAESDGM RRAHIKDAVA LCELFNWLEQ
360 370 380 390 400
EVPKGGVTEI SAADKAEEFR RQQADFVDLS FPTISSTGPN GAIIHYAPVP
410 420 430 440 450
ETNRTLSLDE VYLIDSGAQY KDGTTDVTRT MHFGTPTAYE KECFTYVLKG
460 470 480 490 500
HIAVSAAVFP TGTKGHLLDS FARSALWDSG LDYLHGTGHG VGSFLNVHEG
510 520 530 540 550
PCGISYKTFS DEPLEAGMIV TDEPGYYEDG AFGIRIENVV LVVPAKTKYN
560 570 580 590 600
FNNRGSLTFE PLTLVPIQTK MIDVNALTDK ECDWLNSYHQ TCRDVVGKEL
610 620
QSQGRQEALE WLIRETEPVS RQH
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3UE92 | Q3UE92_MOUSE | Xaa-Pro aminopeptidase 1 | Xpnpep1 | 666 | Annotation score: | ||
S4R1I3 | S4R1I3_MOUSE | Xaa-Pro aminopeptidase 1 | Xpnpep1 | 635 | Annotation score: | ||
A0A494BBG8 | A0A494BBG8_MOUSE | Xaa-Pro aminopeptidase 1 | Xpnpep1 | 587 | Annotation score: | ||
S4R228 | S4R228_MOUSE | Xaa-Pro aminopeptidase 1 | Xpnpep1 | 119 | Annotation score: | ||
S4R167 | S4R167_MOUSE | Xaa-Pro aminopeptidase 1 | Xpnpep1 | 59 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 347 | W → R in AAK48945 (Ref. 1) Curated | 1 | |
Sequence conflicti | 457 | A → T in AAK48945 (Ref. 1) Curated | 1 | |
Sequence conflicti | 544 | P → A in AAK48945 (Ref. 1) Curated | 1 | |
Sequence conflicti | 552 | N → T in AAK48945 (Ref. 1) Curated | 1 | |
Sequence conflicti | 556 | S → T in AAK48945 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF363970 mRNA Translation: AAK48945.1 BC065174 mRNA Translation: AAH65174.1 |
CCDSi | CCDS89415.1 |
RefSeqi | NP_573479.3, NM_133216.3 XP_011245461.1, XM_011247159.2 XP_011245462.1, XM_011247160.1 |
Genome annotation databases
Ensembli | ENSMUST00000236058; ENSMUSP00000157689; ENSMUSG00000025027 ENSMUST00000237294; ENSMUSP00000158390; ENSMUSG00000025027 |
GeneIDi | 170750 |
KEGGi | mmu:170750 |
UCSCi | uc008hwf.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF363970 mRNA Translation: AAK48945.1 BC065174 mRNA Translation: AAH65174.1 |
CCDSi | CCDS89415.1 |
RefSeqi | NP_573479.3, NM_133216.3 XP_011245461.1, XM_011247159.2 XP_011245462.1, XM_011247160.1 |
3D structure databases
SMRi | Q6P1B1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 228414, 14 interactors |
IntActi | Q6P1B1, 4 interactors |
MINTi | Q6P1B1 |
STRINGi | 10090.ENSMUSP00000138250 |
Protein family/group databases
MEROPSi | M24.009 |
PTM databases
iPTMneti | Q6P1B1 |
PhosphoSitePlusi | Q6P1B1 |
SwissPalmi | Q6P1B1 |
Proteomic databases
EPDi | Q6P1B1 |
jPOSTi | Q6P1B1 |
MaxQBi | Q6P1B1 |
PaxDbi | Q6P1B1 |
PRIDEi | Q6P1B1 |
Protocols and materials databases
Antibodypediai | 31645, 185 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000236058; ENSMUSP00000157689; ENSMUSG00000025027 ENSMUST00000237294; ENSMUSP00000158390; ENSMUSG00000025027 |
GeneIDi | 170750 |
KEGGi | mmu:170750 |
UCSCi | uc008hwf.2, mouse |
Organism-specific databases
CTDi | 7511 |
MGIi | MGI:2180003, Xpnpep1 |
Phylogenomic databases
eggNOGi | KOG2413, Eukaryota |
GeneTreei | ENSGT00940000157716 |
InParanoidi | Q6P1B1 |
OrthoDBi | 417805at2759 |
PhylomeDBi | Q6P1B1 |
TreeFami | TF314183 |
Miscellaneous databases
BioGRID-ORCSi | 170750, 1 hit in 16 CRISPR screens |
ChiTaRSi | Xpnpep1, mouse |
PROi | PR:Q6P1B1 |
RNActi | Q6P1B1, protein |
SOURCEi | Search... |
Gene expression databases
ExpressionAtlasi | Q6P1B1, baseline and differential |
Family and domain databases
CDDi | cd01085, APP, 1 hit |
Gene3Di | 3.40.350.10, 2 hits 3.90.230.10, 1 hit |
InterProi | View protein in InterPro IPR029149, Creatin/AminoP/Spt16_NTD IPR036005, Creatinase/aminopeptidase-like IPR000587, Creatinase_N IPR000994, Pept_M24 IPR033740, Pept_M24B IPR032416, Peptidase_M24_C IPR001131, Peptidase_M24B_aminopep-P_CS |
Pfami | View protein in Pfam PF01321, Creatinase_N, 1 hit PF00557, Peptidase_M24, 1 hit PF16188, Peptidase_M24_C, 1 hit |
SUPFAMi | SSF53092, SSF53092, 1 hit SSF55920, SSF55920, 1 hit |
PROSITEi | View protein in PROSITE PS00491, PROLINE_PEPTIDASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | XPP1_MOUSE | |
Accessioni | Q6P1B1Primary (citable) accession number: Q6P1B1 Secondary accession number(s): Q91Y37 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2005 |
Last sequence update: | July 5, 2004 | |
Last modified: | February 10, 2021 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families