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Protein

Mis18-binding protein 1

Gene

MIS18BP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-606279 Deposition of new CENPA-containing nucleosomes at the centromere

Names & Taxonomyi

Protein namesi
Recommended name:
Mis18-binding protein 1
Alternative name(s):
Kinetochore-associated protein KNL-2 homolog
Short name:
HsKNL-2
P243
Gene namesi
Name:MIS18BP1
Synonyms:C14orf106, KIAA1903, KNL2, M18BP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000129534.13
HGNCiHGNC:20190 MIS18BP1
neXtProtiNX_Q6P0N0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000129534
PharmGKBiPA134927557

Polymorphism and mutation databases

BioMutaiMIS18BP1
DMDMi68052324

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001971401 – 1132Mis18-binding protein 1Add BLAST1132

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki7Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei9PhosphoserineCombined sources1
Cross-linki65Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei110PhosphoserineCombined sources1
Modified residuei131PhosphoserineCombined sources1
Modified residuei135PhosphoserineCombined sources1
Modified residuei172PhosphoserineCombined sources1
Modified residuei192PhosphoserineCombined sources1
Cross-linki211Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki262Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei299PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Cross-linki534Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki612Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki639Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki647Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei653PhosphothreonineCombined sources1
Cross-linki727Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki742Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei772PhosphoserineCombined sources1
Modified residuei773PhosphoserineCombined sources1
Modified residuei821PhosphothreonineCombined sources1
Modified residuei824PhosphoserineCombined sources1
Cross-linki840Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei860PhosphoserineCombined sources1
Cross-linki899Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki956Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki964Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki983Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei1008PhosphoserineCombined sources1
Cross-linki1079Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei1086PhosphoserineCombined sources1
Modified residuei1087PhosphothreonineCombined sources1
Modified residuei1089PhosphothreonineCombined sources1
Modified residuei1104PhosphoserineCombined sources1
Modified residuei1116PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ6P0N0
MaxQBiQ6P0N0
PaxDbiQ6P0N0
PeptideAtlasiQ6P0N0
PRIDEiQ6P0N0
ProteomicsDBi66813
66814 [Q6P0N0-2]

PTM databases

iPTMnetiQ6P0N0
PhosphoSitePlusiQ6P0N0

Expressioni

Gene expression databases

BgeeiENSG00000129534 Expressed in 182 organ(s), highest expression level in intestine
CleanExiHS_C14orf106
ExpressionAtlasiQ6P0N0 baseline and differential
GenevisibleiQ6P0N0 HS

Organism-specific databases

HPAiHPA006504

Interactioni

Subunit structurei

Interacts (via N-terminus) with FLNA (via N-terminus) (By similarity). Interacts with SP1 (Probable). Interacts with MIS18A. Identified in a complex containing MIS18A, OIP5/MIS18B, MIS18BP1, RBBP7 and RBBP4.By similarityCurated2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
OIP5O434823EBI-9870821,EBI-536879

Protein-protein interaction databases

BioGridi120601, 22 interactors
ComplexPortaliCPX-3272 Mis18 complex
CORUMiQ6P0N0
IntActiQ6P0N0, 8 interactors
STRINGi9606.ENSP00000309790

Structurei

Secondary structure

11132
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ6P0N0
SMRiQ6P0N0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6P0N0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini383 – 469SANTAAdd BLAST87
Domaini875 – 930SANTPROSITE-ProRule annotationAdd BLAST56

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1010 – 1013Poly-Asp4

Phylogenomic databases

eggNOGiENOG410II9U Eukaryota
ENOG41120B5 LUCA
GeneTreeiENSGT00390000007395
HOGENOMiHOG000113431
InParanoidiQ6P0N0
OMAiNRNDCDK
OrthoDBiEOG091G050V
PhylomeDBiQ6P0N0
TreeFamiTF106401

Family and domain databases

CDDicd00167 SANT, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR039110 KNL2-like
IPR001005 SANT/Myb
IPR017884 SANT_dom
IPR015216 SANTA
PANTHERiPTHR16124 PTHR16124, 1 hit
PfamiView protein in Pfam
PF00249 Myb_DNA-binding, 1 hit
PF09133 SANTA, 1 hit
SMARTiView protein in SMART
SM00717 SANT, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS51293 SANT, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q6P0N0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MIATPLKHSR IYLPPEASSQ RRNLPMDAIF FDSIPSGTLT PVKDLVKYQN
60 70 80 90 100
SSLKLNDHKK NQFLKMTTFN NKNIFQSTML TEATTSNSSL DISAIKPNKD
110 120 130 140 150
GLKNKANYES PGKIFLRMKE KVLRDKQEQP SRNSSLLEPQ KSGNNETFTP
160 170 180 190 200
NRVEKKKLQH TYLCEEKENN KSFQSDDSSL RASVQGVPLE SSNNDIFLPV
210 220 230 240 250
KQKIQCQQEK KAPLHNLTYE LPTLNQEQEN FLAVEARNKT LTRAQLAKQI
260 270 280 290 300
FHSKESIVAT TKSKKDTFVL ESVDSADEQF QNTNAETLST NCIPIKNGSL
310 320 330 340 350
LMVSDSERTT EGTSQQKVKE GNGKTVPGET GLPGSMKDTC KIVLATPRLH
360 370 380 390 400
ITIPRRSKRN ISKLSPPRIF QTVTNGLKKN QVVQLQEWMI KSINNNTAIC
410 420 430 440 450
VEGKLIDVTN IYWHSNVIIE RIEHNKLRTI SGNVYILKGM IDQISMKEAG
460 470 480 490 500
YPNYLIRKFM FGFPENWKEH IDNFLEQLRA GEKNREKTKQ KQKTGRSVRD
510 520 530 540 550
IRKSMKNDAR ENQTDTAQRA TTTYDFDCDN LELKSNKHSE SPGATELNMC
560 570 580 590 600
HSNCQNKPTL RFPDDQVNNT IQNGGGDDLS NQELIGKKEY KMSSKKLKIG
610 620 630 640 650
ERTNERIIKS QKQETTEELD VSIDILTSRE QFFSDEERKY MAINQKKAYI
660 670 680 690 700
LVTPLKSRKV IEQRCMRYNL SAGTIKAVTD FVIPECQKKS PISKSMGTLE
710 720 730 740 750
NTFEGHKSKN KEDCDERDLL TVNRKIKISN LEKEQMLTSD FKKNTRLLPK
760 770 780 790 800
LKKIENQVAM SFYKHQSSPD LSSEESETEK EIKRKAEVKK TKAGNTKEAV
810 820 830 840 850
VHLRKSTRNT SNIPVILEPE TEESENEFYI KQKKARPSVK ETLQKSGVRK
860 870 880 890 900
EFPITEAVGS DKTNRHPLEC LPGLIQDKEW NEKELQKLHC AFASLPKHKP
910 920 930 940 950
GFWSEVAAAV GSRSPEECQR KYMENPRGKG SQKHVTKKKP ANSKGQNGKR
960 970 980 990 1000
GDADQKQTIK ITAKVGTLKR KQQMREFLEQ LPKDDHDDFF STTPLQHQRI
1010 1020 1030 1040 1050
LLPSFQDSED DDDILPNMDK NPTTPSSVIF PLVKTPQCQH VSPGMLGSIN
1060 1070 1080 1090 1100
RNDCDKYVFR MQKYHKSNGG IVWGNIKKKL VETDFSTPTP RRKTPFNTDL
1110 1120 1130
GENSGIGKLF TNAVESLDEE EKDYYFSNSD SA
Length:1,132
Mass (Da):129,085
Last modified:July 5, 2004 - v1
Checksum:i92F2787CD4687059
GO
Isoform 2 (identifier: Q6P0N0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     391-1132: Missing.

Note: No experimental confirmation available.
Show »
Length:390
Mass (Da):43,994
Checksum:iB4B1B945CE91C8A8
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G5E9K5G5E9K5_HUMAN
Chromosome 14 open reading frame 10...
MIS18BP1 C14orf106, hCG_1812025
496Annotation score:
H7C1S6H7C1S6_HUMAN
Mis18-binding protein 1
MIS18BP1
195Annotation score:
C9J2Q8C9J2Q8_HUMAN
Mis18-binding protein 1
MIS18BP1
155Annotation score:
H0YJM1H0YJM1_HUMAN
Mis18-binding protein 1
MIS18BP1
78Annotation score:

Sequence cautioni

The sequence AK002048 differs from that shown. Reason: Frameshift at position 157.Curated
The sequence BAB67796 differs from that shown. Reason: Frameshift at position 154.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti510R → Q in BAB67796 (PubMed:11572484).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050184164C → R. Corresponds to variant dbSNP:rs1269008Ensembl.1
Natural variantiVAR_050185347P → R. Corresponds to variant dbSNP:rs34168608Ensembl.1
Natural variantiVAR_050186583E → D. Corresponds to variant dbSNP:rs34101857Ensembl.1
Natural variantiVAR_050187851E → Q. Corresponds to variant dbSNP:rs34402741Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014105391 – 1132Missing in isoform 2. 1 PublicationAdd BLAST742

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB067490 mRNA Translation: BAB67796.1 Frameshift.
AK002048 mRNA No translation available.
AL121809 Genomic DNA No translation available.
CH471078 Genomic DNA Translation: EAW65778.1
CH471078 Genomic DNA Translation: EAW65779.1
CH471078 Genomic DNA Translation: EAW65780.1
BC051885 mRNA Translation: AAH51885.1
BC065544 mRNA Translation: AAH65544.1
AJ242977 mRNA Translation: CAB45134.1
CCDSiCCDS9684.1 [Q6P0N0-1]
RefSeqiNP_060823.3, NM_018353.4 [Q6P0N0-1]
XP_005267890.1, XM_005267833.4 [Q6P0N0-1]
XP_016876914.1, XM_017021425.1 [Q6P0N0-1]
UniGeneiHs.732769

Genome annotation databases

EnsembliENST00000310806; ENSP00000309790; ENSG00000129534 [Q6P0N0-1]
GeneIDi55320
KEGGihsa:55320
UCSCiuc001wwf.3 human [Q6P0N0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB067490 mRNA Translation: BAB67796.1 Frameshift.
AK002048 mRNA No translation available.
AL121809 Genomic DNA No translation available.
CH471078 Genomic DNA Translation: EAW65778.1
CH471078 Genomic DNA Translation: EAW65779.1
CH471078 Genomic DNA Translation: EAW65780.1
BC051885 mRNA Translation: AAH51885.1
BC065544 mRNA Translation: AAH65544.1
AJ242977 mRNA Translation: CAB45134.1
CCDSiCCDS9684.1 [Q6P0N0-1]
RefSeqiNP_060823.3, NM_018353.4 [Q6P0N0-1]
XP_005267890.1, XM_005267833.4 [Q6P0N0-1]
XP_016876914.1, XM_017021425.1 [Q6P0N0-1]
UniGeneiHs.732769

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGXNMR-A877-936[»]
ProteinModelPortaliQ6P0N0
SMRiQ6P0N0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120601, 22 interactors
ComplexPortaliCPX-3272 Mis18 complex
CORUMiQ6P0N0
IntActiQ6P0N0, 8 interactors
STRINGi9606.ENSP00000309790

PTM databases

iPTMnetiQ6P0N0
PhosphoSitePlusiQ6P0N0

Polymorphism and mutation databases

BioMutaiMIS18BP1
DMDMi68052324

Proteomic databases

EPDiQ6P0N0
MaxQBiQ6P0N0
PaxDbiQ6P0N0
PeptideAtlasiQ6P0N0
PRIDEiQ6P0N0
ProteomicsDBi66813
66814 [Q6P0N0-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310806; ENSP00000309790; ENSG00000129534 [Q6P0N0-1]
GeneIDi55320
KEGGihsa:55320
UCSCiuc001wwf.3 human [Q6P0N0-1]

Organism-specific databases

CTDi55320
EuPathDBiHostDB:ENSG00000129534.13
GeneCardsiMIS18BP1
HGNCiHGNC:20190 MIS18BP1
HPAiHPA006504
neXtProtiNX_Q6P0N0
OpenTargetsiENSG00000129534
PharmGKBiPA134927557
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410II9U Eukaryota
ENOG41120B5 LUCA
GeneTreeiENSGT00390000007395
HOGENOMiHOG000113431
InParanoidiQ6P0N0
OMAiNRNDCDK
OrthoDBiEOG091G050V
PhylomeDBiQ6P0N0
TreeFamiTF106401

Enzyme and pathway databases

ReactomeiR-HSA-606279 Deposition of new CENPA-containing nucleosomes at the centromere

Miscellaneous databases

ChiTaRSiMIS18BP1 human
EvolutionaryTraceiQ6P0N0
GeneWikiiC14orf106
GenomeRNAii55320
PROiPR:Q6P0N0

Gene expression databases

BgeeiENSG00000129534 Expressed in 182 organ(s), highest expression level in intestine
CleanExiHS_C14orf106
ExpressionAtlasiQ6P0N0 baseline and differential
GenevisibleiQ6P0N0 HS

Family and domain databases

CDDicd00167 SANT, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR039110 KNL2-like
IPR001005 SANT/Myb
IPR017884 SANT_dom
IPR015216 SANTA
PANTHERiPTHR16124 PTHR16124, 1 hit
PfamiView protein in Pfam
PF00249 Myb_DNA-binding, 1 hit
PF09133 SANTA, 1 hit
SMARTiView protein in SMART
SM00717 SANT, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS51293 SANT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiM18BP_HUMAN
AccessioniPrimary (citable) accession number: Q6P0N0
Secondary accession number(s): D3DSA7
, Q86V14, Q96PY4, Q9NUR5, Q9Y4X9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
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