UniProtKB - Q6NZP1 (ZRAB3_MOUSE)
DNA annealing helicase and endonuclease ZRANB3
Zranb3
Functioni
DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks.
By similarityMiscellaneous
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 59 – 66 | ATPPROSITE-ProRule annotation | 8 | |
Zinc fingeri | 617 – 646 | RanBP2-typePROSITE-ProRule annotationAdd BLAST | 30 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP-dependent DNA/DNA annealing activity Source: UniProtKB
- endodeoxyribonuclease activity Source: UniProtKB
- helicase activity Source: UniProtKB-KW
- K63-linked polyubiquitin modification-dependent protein binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cellular response to DNA damage stimulus Source: UniProtKB
- DNA repair Source: UniProtKB
- DNA rewinding Source: UniProtKB
- negative regulation of DNA recombination Source: UniProtKB
- replication fork processing Source: UniProtKB
- replication fork protection Source: UniProtKB
- response to UV Source: UniProtKB
Keywordsi
Molecular function | Endonuclease, Helicase, Hydrolase, Multifunctional enzyme, Nuclease |
Biological process | DNA damage, DNA repair |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Zranb3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1918362, Zranb3 |
VEuPathDBi | HostDB:ENSMUSG00000036086 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Chromosome By similarity
Note: Following DNA damage, recruited to sites of DNA damage and stalled replication forks by polyubiquitinated PCNA.By similarity
Nucleus
- nuclear replication fork Source: UniProtKB
- nucleoplasm Source: MGI
Keywords - Cellular componenti
Chromosome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000278183 | 1 – 1069 | DNA annealing helicase and endonuclease ZRANB3Add BLAST | 1069 |
Proteomic databases
EPDi | Q6NZP1 |
MaxQBi | Q6NZP1 |
PaxDbi | Q6NZP1 |
PeptideAtlasi | Q6NZP1 |
PRIDEi | Q6NZP1 |
ProteomicsDBi | 275160 |
PTM databases
iPTMneti | Q6NZP1 |
PhosphoSitePlusi | Q6NZP1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000036086, Expressed in dorsal pancreas and 205 other tissues |
Genevisiblei | Q6NZP1, MM |
Interactioni
Subunit structurei
Interacts (via PIP-box and RanBP2-type zinc finger) with PCNA (when PCNA is polyubiquitinated via 'Lys-63'-linked polyubiquitin).
By similarityGO - Molecular functioni
- K63-linked polyubiquitin modification-dependent protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 230505, 2 interactors |
STRINGi | 10090.ENSMUSP00000083806 |
Miscellaneous databases
RNActi | Q6NZP1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 46 – 208 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 163 | |
Domaini | 325 – 485 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 161 | |
Domaini | 1001 – 1041 | HNHAdd BLAST | 41 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 46 – 481 | DNA annealing helicase activityBy similarityAdd BLAST | 436 | |
Regioni | 648 – 720 | DisorderedSequence analysisAdd BLAST | 73 | |
Regioni | 1001 – 1069 | Endonuclease activityBy similarityAdd BLAST | 69 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 157 – 160 | DEAH box | 4 | |
Motifi | 518 – 525 | PIP-box | 8 | |
Motifi | 1064 – 1068 | APIM motif | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 651 – 667 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 677 – 703 | Basic and acidic residuesSequence analysisAdd BLAST | 27 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 617 – 646 | RanBP2-typePROSITE-ProRule annotationAdd BLAST | 30 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG1000, Eukaryota |
GeneTreei | ENSGT00940000158559 |
HOGENOMi | CLU_004251_0_0_1 |
InParanoidi | Q6NZP1 |
OMAi | PGFSEWF |
OrthoDBi | 1082831at2759 |
PhylomeDBi | Q6NZP1 |
TreeFami | TF354227 |
Family and domain databases
CDDi | cd00085, HNHc, 1 hit |
Gene3Di | 3.40.50.10810, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR002711, HNH IPR003615, HNH_nuc IPR027417, P-loop_NTPase IPR038718, SNF2-like_sf IPR000330, SNF2_N IPR001876, Znf_RanBP2 IPR036443, Znf_RanBP2_sf |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF01844, HNH, 1 hit PF00176, SNF2-rel_dom, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00507, HNHc, 1 hit SM00547, ZnF_RBZ, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF90209, SSF90209, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS01358, ZF_RANBP2_1, 1 hit PS50199, ZF_RANBP2_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MPTAGSKKKA PTPQISCLTS ESYTQLDFLP DKLRTKLLPF QKDGIVFALR
60 70 80 90 100
RDGRCMVADE MGLGKTIQAI AIAYFYKEEW PLLIVVPSSL RYPWIEELEK
110 120 130 140 150
WIPELEPEEI NVVMNKTDIG RIPGSRVTVL GYGLLTTDAE TLLDALNTQN
160 170 180 190 200
FRVVIVDESH YMKSRTAARS KILLPMVQKA RRAILLTGTP ALGRPEELFM
210 220 230 240 250
QIEALFPQKF GTWIEYAKRY CNAHVRYFGK RRQWDCRGAS NLSELHQLLN
260 270 280 290 300
DIMIRRLKSE VLSQLPPKVR QRIPFDLPPA AVKELNASFE EWQKLMRAPN
310 320 330 340 350
SGAMETVMGL ITRMFKQTAI AKAGAVKDYI KMLLQNDSLK FLVFAHHLSM
360 370 380 390 400
LQACTEAVIE SKSRYIRIDG SVPSSERIHL VNQFQKDPDT RVAILSIQAA
410 420 430 440 450
GQGLTFTAAS HVVFAELYWD PGHIKQAEDR AHRIGQCSSV NIHYLIANGT
460 470 480 490 500
LDSLMWAMLN RKAQVTGSTL NGRKEKLQAT EDDKEKWGFL QFAEAWTPSD
510 520 530 540 550
SFEELKDSVF THFEKEKQHD IRSFFLPKLK KRQLETTCDD PEAFKEKITV
560 570 580 590 600
ASDPRKMATS DSTADKNGCE PEAKRLKSLS TEDHSSALEE GPSLQARATS
610 620 630 640 650
MEVVHEVKPP LASPALPEKG WQCGFCTFLN NPGLPYCEMC ENPRSRAAGR
660 670 680 690 700
NHLQDNNKND EDAAQESTSK SDQAGLECER QCPERLEAEQ SANSKEEALE
710 720 730 740 750
GGGEDRLPSQ PEIGQLNNSG TLPVRETFMF CASRNTDRIH LYTKDGKPMN
760 770 780 790 800
CNFIPLDIKL DLWEDLPATF QLKQNRSLIL RFVREWSSLT AMKQRVLRKS
810 820 830 840 850
GQLFCSPLLA SEEITKQQAK ENNTRRYITK EDVAKASMNK VKSDGGHIRL
860 870 880 890 900
ITKESMTQDS SLKKIDSACV PSLNPCPADL TVEPSPSKGY IQAVDKEGRP
910 920 930 940 950
LCLRCQHPTC QPEQTAKASA WDSRFCSLKC QEEFWIRSNN SYLRAQVFAT
960 970 980 990 1000
EHGVCQHCGV DAQELFLRMR DAPKSHRKSL LNAAWTAKLP LEQLNEMLRN
1010 1020 1030 1040 1050
PGEGHFWQVD HIRPVYEGGG QCSLDNLQTL CTVCHKERTA QQAKERSQVR
1060
RLSLATKHGS DITRFLVKK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 311 | I → M in BAC34042 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 435 | G → R in BAC38978 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 865 | I → L in AAI17923 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 865 | I → L in AAI17922 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 887 | S → T in AAI17923 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 887 | S → T in AAI17922 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 957 | H → R in AAI17923 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 957 | H → R in AAI17922 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 968 | R → W in AAI17923 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 968 | R → W in AAI17922 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK050039 mRNA Translation: BAC34042.1 AK083641 mRNA Translation: BAC38978.1 BC066035 mRNA Translation: AAH66035.1 BC117921 mRNA Translation: AAI17922.1 BC117922 mRNA Translation: AAI17923.1 |
CCDSi | CCDS35697.1 |
RefSeqi | NP_001272874.1, NM_001285945.1 NP_081954.1, NM_027678.3 XP_006529475.1, XM_006529412.2 |
Genome annotation databases
Ensembli | ENSMUST00000086614; ENSMUSP00000083806; ENSMUSG00000036086 ENSMUST00000112538; ENSMUSP00000108157; ENSMUSG00000036086 |
GeneIDi | 226409 |
KEGGi | mmu:226409 |
UCSCi | uc007cld.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK050039 mRNA Translation: BAC34042.1 AK083641 mRNA Translation: BAC38978.1 BC066035 mRNA Translation: AAH66035.1 BC117921 mRNA Translation: AAI17922.1 BC117922 mRNA Translation: AAI17923.1 |
CCDSi | CCDS35697.1 |
RefSeqi | NP_001272874.1, NM_001285945.1 NP_081954.1, NM_027678.3 XP_006529475.1, XM_006529412.2 |
3D structure databases
SMRi | Q6NZP1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230505, 2 interactors |
STRINGi | 10090.ENSMUSP00000083806 |
PTM databases
iPTMneti | Q6NZP1 |
PhosphoSitePlusi | Q6NZP1 |
Proteomic databases
EPDi | Q6NZP1 |
MaxQBi | Q6NZP1 |
PaxDbi | Q6NZP1 |
PeptideAtlasi | Q6NZP1 |
PRIDEi | Q6NZP1 |
ProteomicsDBi | 275160 |
Protocols and materials databases
Antibodypediai | 56175, 78 antibodies from 18 providers |
Genome annotation databases
Ensembli | ENSMUST00000086614; ENSMUSP00000083806; ENSMUSG00000036086 ENSMUST00000112538; ENSMUSP00000108157; ENSMUSG00000036086 |
GeneIDi | 226409 |
KEGGi | mmu:226409 |
UCSCi | uc007cld.2, mouse |
Organism-specific databases
CTDi | 84083 |
MGIi | MGI:1918362, Zranb3 |
VEuPathDBi | HostDB:ENSMUSG00000036086 |
Phylogenomic databases
eggNOGi | KOG1000, Eukaryota |
GeneTreei | ENSGT00940000158559 |
HOGENOMi | CLU_004251_0_0_1 |
InParanoidi | Q6NZP1 |
OMAi | PGFSEWF |
OrthoDBi | 1082831at2759 |
PhylomeDBi | Q6NZP1 |
TreeFami | TF354227 |
Miscellaneous databases
BioGRID-ORCSi | 226409, 3 hits in 100 CRISPR screens |
ChiTaRSi | Zranb3, mouse |
PROi | PR:Q6NZP1 |
RNActi | Q6NZP1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036086, Expressed in dorsal pancreas and 205 other tissues |
Genevisiblei | Q6NZP1, MM |
Family and domain databases
CDDi | cd00085, HNHc, 1 hit |
Gene3Di | 3.40.50.10810, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR002711, HNH IPR003615, HNH_nuc IPR027417, P-loop_NTPase IPR038718, SNF2-like_sf IPR000330, SNF2_N IPR001876, Znf_RanBP2 IPR036443, Znf_RanBP2_sf |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF01844, HNH, 1 hit PF00176, SNF2-rel_dom, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00507, HNHc, 1 hit SM00547, ZnF_RBZ, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF90209, SSF90209, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS01358, ZF_RANBP2_1, 1 hit PS50199, ZF_RANBP2_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ZRAB3_MOUSE | |
Accessioni | Q6NZP1Primary (citable) accession number: Q6NZP1 Secondary accession number(s): Q148X9, Q8BJJ3, Q8BWT6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 20, 2007 |
Last sequence update: | July 5, 2004 | |
Last modified: | February 23, 2022 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families