UniProtKB - Q6NZL6 (TONSL_MOUSE)
Protein
Tonsoku-like protein
Gene
Tonsl
Organism
Mus musculus (Mouse)
Status
Functioni
Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold (By similarity).By similarity
GO - Molecular functioni
- histone binding Source: UniProtKB
GO - Biological processi
- double-strand break repair via homologous recombination Source: UniProtKB
- replication fork processing Source: UniProtKB
Keywordsi
Biological process | DNA damage, DNA repair |
Names & Taxonomyi
Protein namesi | Recommended name: Tonsoku-like proteinAlternative name(s): Inhibitor of kappa B-related protein Short name: I-kappa-B-related protein Short name: IkappaBR NF-kappa-B inhibitor-like protein 2 Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2 |
Gene namesi | Name:Tonsl Synonyms:Ikbr, Nfkbil2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919999, Tonsl |
Subcellular locationi
Nucleus
- nuclear body Source: MGI
- nuclear replication fork Source: UniProtKB
- nucleoplasm Source: MGI
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 924 | R → W: Homozygous mice are not viable. Embryos show early lethality along with fetal growth restriction and lack of visible blood vessels in yolk sacs. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000326635 | 1 – 1363 | Tonsoku-like proteinAdd BLAST | 1363 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 796 | Omega-N-methylarginineCombined sources | 1 |
Keywords - PTMi
MethylationProteomic databases
EPDi | Q6NZL6 |
MaxQBi | Q6NZL6 |
PaxDbi | Q6NZL6 |
PeptideAtlasi | Q6NZL6 |
PRIDEi | Q6NZL6 |
PTM databases
iPTMneti | Q6NZL6 |
PhosphoSitePlusi | Q6NZL6 |
Interactioni
Subunit structurei
Component of the MMS22L-TONSL complex, a complex at least composed of MMS22L and TONSL/NFKBIL2.
Interacts with the MCM complex, the FACT complex and the RPA complex. Binds histones (By similarity).
By similarityGO - Molecular functioni
- histone binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000129597 |
Miscellaneous databases
RNActi | Q6NZL6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 27 – 60 | TPR 1Add BLAST | 34 | |
Repeati | 67 – 100 | TPR 2Add BLAST | 34 | |
Repeati | 107 – 147 | TPR 3Add BLAST | 41 | |
Repeati | 162 – 195 | TPR 4Add BLAST | 34 | |
Repeati | 202 – 235 | TPR 5Add BLAST | 34 | |
Repeati | 242 – 275 | TPR 6Add BLAST | 34 | |
Repeati | 311 – 344 | TPR 7Add BLAST | 34 | |
Repeati | 352 – 385 | TPR 8Add BLAST | 34 | |
Repeati | 528 – 557 | ANK 1Add BLAST | 30 | |
Repeati | 561 – 590 | ANK 2Add BLAST | 30 | |
Repeati | 597 – 626 | ANK 3Add BLAST | 30 | |
Repeati | 1060 – 1081 | LRR 1Add BLAST | 22 | |
Repeati | 1088 – 1108 | LRR 2Add BLAST | 21 | |
Repeati | 1119 – 1140 | LRR 3Add BLAST | 22 | |
Repeati | 1147 – 1168 | LRR 4Add BLAST | 22 | |
Repeati | 1179 – 1199 | LRR 5Add BLAST | 21 | |
Repeati | 1206 – 1214 | LRR 6 | 9 | |
Repeati | 1238 – 1261 | LRR 7Add BLAST | 24 | |
Repeati | 1266 – 1287 | LRR 8Add BLAST | 22 | |
Repeati | 1296 – 1317 | LRR 9Add BLAST | 22 | |
Repeati | 1322 – 1343 | LRR 10Add BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 466 – 509 | Glu-richAdd BLAST | 44 |
Domaini
The ANK repeats mediate the interaction with the MCM complex and histones, while the LRR repeats mediate the interaction with MMS22L.By similarity
Sequence similaritiesi
Belongs to the Tonsoku family.Curated
Keywords - Domaini
ANK repeat, Leucine-rich repeat, Repeat, TPR repeatPhylogenomic databases
eggNOGi | KOG0504, Eukaryota KOG4308, Eukaryota |
InParanoidi | Q6NZL6 |
OrthoDBi | 56880at2759 |
PhylomeDBi | Q6NZL6 |
Family and domain databases
Gene3Di | 1.25.40.10, 2 hits 1.25.40.20, 1 hit 3.80.10.10, 2 hits |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR001611, Leu-rich_rpt IPR032675, LRR_dom_sf IPR013026, TPR-contain_dom IPR011990, TPR-like_helical_dom_sf IPR019734, TPR_repeat |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF13516, LRR_6, 2 hits PF13176, TPR_7, 1 hit |
PRINTSi | PR01415, ANKYRIN |
SMARTi | View protein in SMART SM00248, ANK, 3 hits SM00028, TPR, 5 hits |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF48452, SSF48452, 3 hits |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 3 hits PS50293, TPR_REGION, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q6NZL6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTLEQELRQL SKAKTRAQRN GQLREEAAYC HQLGELLASH GRFKDALEEH
60 70 80 90 100
QQELHLLESV QDTLGCAVAH RKIGERLAEM ENYSAALKHQ HLYLDLAGSL
110 120 130 140 150
SNHTELQRAW ATIGRTHLDI YDHCQSRDSL LQAQAAFEKS LAIVDEKLEG
160 170 180 190 200
MLTQRELSEM RTRLYLNLGL TCESLQQTAL CNNYFKKSIF LAEQNHLYED
210 220 230 240 250
LFRARYNLGA IHWRGGQHSQ AMRCLEGARE CARAMKMRFM ESECCVLVSQ
260 270 280 290 300
VLQDLGDFLA AKRALKKAYR LGSQKPNQRV TVCQSLKYVL AVIQLQQQLE
310 320 330 340 350
EAEGNDLQGA MAICEQLGDL FSKAGDFPKA AEAYQKQLHL AELLNRPDLE
360 370 380 390 400
LAVIHVSLAT TLGDMKDHRK AVHHYEEELR LRKGNALEEA KTWFNIALSR
410 420 430 440 450
EEAGDAYELL APCFQKAFCC AQQAQRFQLQ RQILQHLYTV QLKLQPQEAR
460 470 480 490 500
DTEIRLQELS MAKDTEEEEE EEEEEEEEAS EAPETSELEL SESEDDADGL
510 520 530 540 550
SQQLEEDEEL QGCVGRRKVN KWNRRNDMGE TLLHRACIEG QLRRVQDLVK
560 570 580 590 600
QGHPLNPRDY CGWTPLHEAC NYGHLEIVRF LLDHGAAVDD PGGQGCDGIT
610 620 630 640 650
PLHDALNCGH FEVAELLIER GASVTLRTRK GLSPLETLQQ WVKLYFRDLD
660 670 680 690 700
LETRQKAATM EERLQMASSG QASRSSPALQ TIPSNHLFDP ETSPPSSPCP
710 720 730 740 750
EPSSYTPRPP EASPAPAKVF LEETVSAVSR PRKTRHRPTS SSSSSEDEDN
760 770 780 790 800
PSPCRPSQKR LRHTTQQGEV KIPDPPKSRE TATSSACRAA YQAAIRGVGS
810 820 830 840 850
AQSRRLVPSL PRGSEEVPAP KTALIPEEEY LAGEWLEVDT PLTRSGRPST
860 870 880 890 900
SVSDYERCPA RPRTRVKQSR LTSLDGWCAR TQAGDGSLNA EPAENPSVPR
910 920 930 940 950
TSGPNKENYA AGQPLLLVQP PPIRVRVQIQ DNLFLIPVPQ SDIRPVAWLT
960 970 980 990 1000
EQAAQRYFQT CGLLPRLTLR KDGALLAPQD PIPDVLQSND EVLAEVTSWD
1010 1020 1030 1040 1050
LPPLKDRYRR ACLSLGQGEH QQVLHAMDHQ SSSPSFSACS LALCQAQLTP
1060 1070 1080 1090 1100
LLRALKLHTA LRELRLAGNR LGDACATELL ATLGTTPNLV LLDLSSNHLG
1110 1120 1130 1140 1150
QEGLRQLVEG SSGQAALQNL EELDLSMNPL GDGCGQALAS LLRACPMLST
1160 1170 1180 1190 1200
LRLQACGFSS SFFLSHQAAL GGAFQDAVHL KTLSLSYNLL GAPALARVLQ
1210 1220 1230 1240 1250
TLPACTLKRL DLSSVAASKS NSGIIEPVIK YLTKEGCALA HLTLSANCLG
1260 1270 1280 1290 1300
DKAVRELSRC LPCCPSLTSL DLSANPEVSC ASLEELLSAL QERSQGLSFL
1310 1320 1330 1340 1350
GLSGCSIQGP LNSDLWDKIF VQLQELQLCT KDLSTKDRDS VCQRLPEGAC
1360
TMDQSSKLFF KCL
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3UW83 | G3UW83_MOUSE | NF-kappa-B inhibitor-like protein 2 | Tonsl | 1,363 | Annotation score: | ||
E9Q2M2 | E9Q2M2_MOUSE | Tonsoku-like protein | Tonsl | 417 | Annotation score: | ||
F7A0T4 | F7A0T4_MOUSE | Tonsoku-like protein | Tonsl | 86 | Annotation score: | ||
E9PZL7 | E9PZL7_MOUSE | Tonsoku-like protein | Tonsl | 64 | Annotation score: |
Sequence cautioni
The sequence AAH66068 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAC25399 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence CAC08218 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 293 – 294 | IQ → NR in CAC08218 (Ref. 3) Curated | 2 | |
Sequence conflicti | 1106 – 1107 | QL → HV in BAC25399 (PubMed:16141072).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC156550 Genomic DNA No translation available. AC157566 Genomic DNA No translation available. BC066068 mRNA Translation: AAH66068.1 Different initiation. AJ294539 Genomic DNA Translation: CAC08218.1 Sequence problems. AK013272 mRNA Translation: BAC25399.1 Different initiation. |
CCDSi | CCDS27580.2 |
RefSeqi | NP_898914.3, NM_183091.3 |
Genome annotation databases
GeneIDi | 72749 |
KEGGi | mmu:72749 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC156550 Genomic DNA No translation available. AC157566 Genomic DNA No translation available. BC066068 mRNA Translation: AAH66068.1 Different initiation. AJ294539 Genomic DNA Translation: CAC08218.1 Sequence problems. AK013272 mRNA Translation: BAC25399.1 Different initiation. |
CCDSi | CCDS27580.2 |
RefSeqi | NP_898914.3, NM_183091.3 |
3D structure databases
SMRi | Q6NZL6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000129597 |
PTM databases
iPTMneti | Q6NZL6 |
PhosphoSitePlusi | Q6NZL6 |
Proteomic databases
EPDi | Q6NZL6 |
MaxQBi | Q6NZL6 |
PaxDbi | Q6NZL6 |
PeptideAtlasi | Q6NZL6 |
PRIDEi | Q6NZL6 |
Genome annotation databases
GeneIDi | 72749 |
KEGGi | mmu:72749 |
Organism-specific databases
CTDi | 4796 |
MGIi | MGI:1919999, Tonsl |
Phylogenomic databases
eggNOGi | KOG0504, Eukaryota KOG4308, Eukaryota |
InParanoidi | Q6NZL6 |
OrthoDBi | 56880at2759 |
PhylomeDBi | Q6NZL6 |
Miscellaneous databases
BioGRID-ORCSi | 72749, 8 hits in 17 CRISPR screens |
PROi | PR:Q6NZL6 |
RNActi | Q6NZL6, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 1.25.40.10, 2 hits 1.25.40.20, 1 hit 3.80.10.10, 2 hits |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR001611, Leu-rich_rpt IPR032675, LRR_dom_sf IPR013026, TPR-contain_dom IPR011990, TPR-like_helical_dom_sf IPR019734, TPR_repeat |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF13516, LRR_6, 2 hits PF13176, TPR_7, 1 hit |
PRINTSi | PR01415, ANKYRIN |
SMARTi | View protein in SMART SM00248, ANK, 3 hits SM00028, TPR, 5 hits |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF48452, SSF48452, 3 hits |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 3 hits PS50293, TPR_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TONSL_MOUSE | |
Accessioni | Q6NZL6Primary (citable) accession number: Q6NZL6 Secondary accession number(s): Q8BT74, Q9ER45 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 8, 2008 |
Last sequence update: | April 8, 2008 | |
Last modified: | December 2, 2020 | |
This is version 139 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families