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Protein

Replication protein A 70 kDa DNA-binding subunit

Gene

rpa1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi184 – 268OBAdd BLAST85

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA replication
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DRE-110312 Translesion synthesis by REV1
R-DRE-110314 Recognition of DNA damage by PCNA-containing replication complex
R-DRE-110320 Translesion Synthesis by POLH
R-DRE-174437 Removal of the Flap Intermediate from the C-strand
R-DRE-176187 Activation of ATR in response to replication stress
R-DRE-3108214 SUMOylation of DNA damage response and repair proteins
R-DRE-3371453 Regulation of HSF1-mediated heat shock response
R-DRE-5358565 Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha)
R-DRE-5358606 Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta)
R-DRE-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-DRE-5655862 Translesion synthesis by POLK
R-DRE-5656121 Translesion synthesis by POLI
R-DRE-5656169 Termination of translesion DNA synthesis
R-DRE-5685938 HDR through Single Strand Annealing (SSA)
R-DRE-5685942 HDR through Homologous Recombination (HRR)
R-DRE-5693607 Processing of DNA double-strand break ends
R-DRE-5696395 Formation of Incision Complex in GG-NER
R-DRE-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-DRE-5696400 Dual Incision in GG-NER
R-DRE-6782135 Dual incision in TC-NER
R-DRE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-DRE-6783310 Fanconi Anemia Pathway
R-DRE-6804756 Regulation of TP53 Activity through Phosphorylation
R-DRE-68962 Activation of the pre-replicative complex
R-DRE-69166 Removal of the Flap Intermediate
R-DRE-69473 G2/M DNA damage checkpoint

Names & Taxonomyi

Protein namesi
Recommended name:
Replication protein A 70 kDa DNA-binding subunit
Short name:
RP-A p70
Alternative name(s):
Replication factor A protein 1
Short name:
RF-A protein 1
Gene namesi
Name:rpa1
ORF Names:wu:fi14b08
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 15

Organism-specific databases

ZFINiZDB-GENE-030912-3 rpa1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000972651 – 601Replication protein A 70 kDa DNA-binding subunitAdd BLAST601

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei370Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6NY74
PRIDEiQ6NY74

PTM databases

iPTMnetiQ6NY74

Expressioni

Gene expression databases

BgeeiENSDARG00000003938 Expressed in 31 organ(s), highest expression level in testis

Interactioni

Subunit structurei

Component of the heterotrimeric canonical replication protein A complex (RPA).By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000029067

Structurei

3D structure databases

ProteinModelPortaliQ6NY74
SMRiQ6NY74
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0851 Eukaryota
COG1599 LUCA
GeneTreeiENSGT00390000012403
HOGENOMiHOG000162322
HOVERGENiHBG010502
InParanoidiQ6NY74
KOiK07466
OMAiNIYLMDT
OrthoDBiEOG091G02VJ
PhylomeDBiQ6NY74
TreeFamiTF105241

Family and domain databases

CDDicd04476 RPA1_DBD_C, 1 hit
cd04477 RPA1N, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
IPR013955 Rep_factor-A_C
IPR007199 Rep_factor-A_N
IPR031657 REPA_OB_2
IPR004591 Rfa1
PfamiView protein in Pfam
PF04057 Rep-A_N, 1 hit
PF08646 Rep_fac-A_C, 1 hit
PF16900 REPA_OB_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
SUPFAMiSSF50249 SSF50249, 4 hits
TIGRFAMsiTIGR00617 rpa1, 1 hit

Sequencei

Sequence statusi: Complete.

Q6NY74-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTVRLSEGAI ESLSKGTEVN NPILQCVNIR KIDGGNGVSR FRVMMSDGLH
60 70 80 90 100
TMSSFMLSTQ LNPMAEQNQL ATNCVCVLKR SVTNVLKDGR RVVVILDIEV
110 120 130 140 150
LKSADQMPGK IGDPTPYVEG QSKAPSTAPA PTARPLQPQN GSDGSTYRPS
160 170 180 190 200
AQSFGKKPMA APNTPGGSSK VVPIASLNPY QSKWTIRARV TNKSAIRTWS
210 220 230 240 250
NSRGDGKLFS MELVDESGEI RATGFNNEVD KFFSLIEQGK VFYISKGTLK
260 270 280 290 300
IANKQFSSLK NDYEMTLNGE TSIIPCEDSN DVPMLQCDFV SIADLESREK
310 320 330 340 350
DTILDVIGVC KNAEDVARIM TKNSREVSKR NIQLIDMSGR VIQLTMWGSD
360 370 380 390 400
AETFDGSGQP ILAIKGARLS DFGGRSLSTL YSSTVMINPD IPEAYKLRGW
410 420 430 440 450
YDKEGHALDG QSMTELRGPG GGGNTNWKTL AEVKNEHLGH GDKADYFSCI
460 470 480 490 500
ATIVYIRKEN CLYQACPSKD CNKKVVDQQN GMFRCEKCDK EFPDFKYRLM
510 520 530 540 550
LSANIADFGD NQWVTCFQDT AETLLGQNSS YLGQLKDTNE AAFDEVFQHA
560 570 580 590 600
NFNTFVFRNR VKLETYNDES RIKVTVVDAK PVDHREYSKR LIINIRKLAA

Q
Length:601
Mass (Da):66,631
Last modified:July 5, 2004 - v1
Checksum:i420CA417C5E3981B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY648787 mRNA Translation: AAT68105.1
BC066711 mRNA Translation: AAH66711.1
RefSeqiNP_956105.2, NM_199811.2
UniGeneiDr.76790

Genome annotation databases

EnsembliENSDART00000032880; ENSDARP00000029067; ENSDARG00000003938
GeneIDi327491
KEGGidre:327491

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY648787 mRNA Translation: AAT68105.1
BC066711 mRNA Translation: AAH66711.1
RefSeqiNP_956105.2, NM_199811.2
UniGeneiDr.76790

3D structure databases

ProteinModelPortaliQ6NY74
SMRiQ6NY74
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000029067

PTM databases

iPTMnetiQ6NY74

Proteomic databases

PaxDbiQ6NY74
PRIDEiQ6NY74

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000032880; ENSDARP00000029067; ENSDARG00000003938
GeneIDi327491
KEGGidre:327491

Organism-specific databases

CTDi6117
ZFINiZDB-GENE-030912-3 rpa1

Phylogenomic databases

eggNOGiKOG0851 Eukaryota
COG1599 LUCA
GeneTreeiENSGT00390000012403
HOGENOMiHOG000162322
HOVERGENiHBG010502
InParanoidiQ6NY74
KOiK07466
OMAiNIYLMDT
OrthoDBiEOG091G02VJ
PhylomeDBiQ6NY74
TreeFamiTF105241

Enzyme and pathway databases

ReactomeiR-DRE-110312 Translesion synthesis by REV1
R-DRE-110314 Recognition of DNA damage by PCNA-containing replication complex
R-DRE-110320 Translesion Synthesis by POLH
R-DRE-174437 Removal of the Flap Intermediate from the C-strand
R-DRE-176187 Activation of ATR in response to replication stress
R-DRE-3108214 SUMOylation of DNA damage response and repair proteins
R-DRE-3371453 Regulation of HSF1-mediated heat shock response
R-DRE-5358565 Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha)
R-DRE-5358606 Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta)
R-DRE-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-DRE-5655862 Translesion synthesis by POLK
R-DRE-5656121 Translesion synthesis by POLI
R-DRE-5656169 Termination of translesion DNA synthesis
R-DRE-5685938 HDR through Single Strand Annealing (SSA)
R-DRE-5685942 HDR through Homologous Recombination (HRR)
R-DRE-5693607 Processing of DNA double-strand break ends
R-DRE-5696395 Formation of Incision Complex in GG-NER
R-DRE-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-DRE-5696400 Dual Incision in GG-NER
R-DRE-6782135 Dual incision in TC-NER
R-DRE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-DRE-6783310 Fanconi Anemia Pathway
R-DRE-6804756 Regulation of TP53 Activity through Phosphorylation
R-DRE-68962 Activation of the pre-replicative complex
R-DRE-69166 Removal of the Flap Intermediate
R-DRE-69473 G2/M DNA damage checkpoint

Miscellaneous databases

PROiPR:Q6NY74

Gene expression databases

BgeeiENSDARG00000003938 Expressed in 31 organ(s), highest expression level in testis

Family and domain databases

CDDicd04476 RPA1_DBD_C, 1 hit
cd04477 RPA1N, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
IPR013955 Rep_factor-A_C
IPR007199 Rep_factor-A_N
IPR031657 REPA_OB_2
IPR004591 Rfa1
PfamiView protein in Pfam
PF04057 Rep-A_N, 1 hit
PF08646 Rep_fac-A_C, 1 hit
PF16900 REPA_OB_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
SUPFAMiSSF50249 SSF50249, 4 hits
TIGRFAMsiTIGR00617 rpa1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRFA1_DANRE
AccessioniPrimary (citable) accession number: Q6NY74
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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