UniProtKB - Q6NXW6 (RAD17_MOUSE)
Protein
Cell cycle checkpoint protein RAD17
Gene
Rad17
Organism
Mus musculus (Mouse)
Status
Functioni
Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination (By similarity). Essential for embryonic development. May be involved in homologous recombination.By similarity1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 136 – 143 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- chromatin binding Source: GO_Central
- DNA clamp loader activity Source: InterPro
GO - Biological processi
- cellular response to DNA damage stimulus Source: MGI
- DNA damage checkpoint Source: MGI
- DNA repair Source: GO_Central
- mitotic DNA replication checkpoint Source: GO_Central
- multicellular organism development Source: UniProtKB-KW
- negative regulation of DNA replication Source: MGI
- regulation of phosphorylation Source: MGI
Keywordsi
Molecular function | Developmental protein |
Biological process | Cell cycle, DNA damage |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-176187, Activation of ATR in response to replication stress R-MMU-5685938, HDR through Single Strand Annealing (SSA) R-MMU-5693607, Processing of DNA double-strand break ends R-MMU-5693616, Presynaptic phase of homologous DNA pairing and strand exchange R-MMU-6804756, Regulation of TP53 Activity through Phosphorylation R-MMU-69473, G2/M DNA damage checkpoint |
Names & Taxonomyi
Protein namesi | Recommended name: Cell cycle checkpoint protein RAD17 |
Gene namesi | Name:Rad17 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1333807, Rad17 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Note: Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.By similarity
Nucleus
- nuclear chromatin Source: GO_Central
- nucleolus Source: MGI
- nucleoplasm Source: MGI
Other locations
- chromosome, telomeric region Source: Ensembl
- Rad17 RFC-like complex Source: InterPro
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Mice show numerous defects in embryonic development, starting at E8.5.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209949 | 1 – 688 | Cell cycle checkpoint protein RAD17Add BLAST | 688 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 55 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 85 | PhosphoserineBy similarity | 1 | |
Modified residuei | 358 | PhosphoserineBy similarity | 1 | |
Modified residuei | 647 | Phosphoserine; by ATR3 Publications | 1 | |
Modified residuei | 657 | Phosphoserine; by ATR2 Publications | 1 |
Post-translational modificationi
Phosphorylated. Phosphorylation on Ser-647 and Ser-657 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling.3 Publications
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q6NXW6 |
MaxQBi | Q6NXW6 |
PaxDbi | Q6NXW6 |
PRIDEi | Q6NXW6 |
PTM databases
iPTMneti | Q6NXW6 |
PhosphoSitePlusi | Q6NXW6 |
Expressioni
Tissue specificityi
Ubiquitous at low levels. Highly expressed in testis, where it is expressed in spermatogonia, spermatocytes and spermatids, but absent in mature spermatozoa (at protein level).3 Publications
Gene expression databases
Bgeei | ENSMUSG00000021635, Expressed in cleaving embryo and 295 other tissues |
Genevisiblei | Q6NXW6, MM |
Interactioni
Subunit structurei
Part of a DNA-binding complex containing RFC2, RFC3, RFC4 and RFC5.
Interacts with RAD1 and RAD9 within the RAD1-RAD9-HUS1 complex.
Interacts with RAD9B, POLE, SNU13 and MCM7. DNA damage promotes interaction with ATR or ATM and disrupts interaction with the RAD1-RAD9-HUS1 complex (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 202559, 1 interactor |
DIPi | DIP-59315N |
IntActi | Q6NXW6, 2 interactors |
STRINGi | 10090.ENSMUSP00000022136 |
Miscellaneous databases
RNActi | Q6NXW6, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 431 – 688 | Interaction with MCM7By similarityAdd BLAST | 258 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 669 – 676 | Poly-Glu | 8 |
Sequence similaritiesi
Belongs to the rad17/RAD24 family.Curated
Phylogenomic databases
eggNOGi | KOG1970, Eukaryota |
GeneTreei | ENSGT00440000039046 |
HOGENOMi | CLU_018598_0_0_1 |
InParanoidi | Q6NXW6 |
OMAi | EPWVDRY |
OrthoDBi | 674169at2759 |
PhylomeDBi | Q6NXW6 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR004582, Checkpoint_prot_Rad17_Rad24 IPR027417, P-loop_NTPase IPR018324, Rad17/Rad24_fun/met |
PANTHERi | PTHR12172, PTHR12172, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00602, rad24, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q6NXW6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSETFLRPKV SSTKVTDWVA PAFDDFEANT AITTITASSL TFSNSSHRRK
60 70 80 90 100
YLPSTLESNR LSARKRGRLS LEQTHGLETS RERLSDNEPW VDKYKPETQH
110 120 130 140 150
ELAVHKKKIE EVETWLKAQV LEVKPKQGGS VLLITGPPGC GKTTTIKILS
160 170 180 190 200
KELGIQVQEW VNPILPDFQK DDYKELLSLE SNFSVVPYQS QIAVFNDFLL
210 220 230 240 250
RATKYSKLQM LGDDLTTDKK IILVEELPNQ FYRDPNALHE ILRKHVQIGR
260 270 280 290 300
CPLVFIVSDS VSGDNNQRLL FPRNIQEECS VSNISFNPVA PTIMMKFLNR
310 320 330 340 350
IVTIEASKNG EKIIVPNKTS LELLCQGCSG DIRSAINSLQ FSSSKGENSS
360 370 380 390 400
WSKKKRMSLK SDAAISKSKQ KKKHNSTLEN QEIQAIGGKD VSLFLFRALG
410 420 430 440 450
KILYCKRAPL TELDSPRLPA HLSEHDRDTL LVQPEEIVEM SHMPGDFFNL
460 470 480 490 500
YLHQNYIDFF AEVDDLVPAS EFLSFADILG GDWNTRSLLR EYSTSVATRG
510 520 530 540 550
VMHSNKARGF AHCQGGSSFR PLHKPQWFLI QKKYRENCLA AKALFVDFCL
560 570 580 590 600
PALCLQTQLL PYLALLTIPM RNKAQISFIQ DVGRLPLKRS FGRLKMEALT
610 620 630 640 650
DRELGLIDPD SGDESPHSGG QPAQEAPGEP AQAAQNADPE TWSLPLSQNS
660 670 680
GSDLPASQPQ PFSSKVDMEE EEEEEEDIII EDYDSEET
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A286YCW1 | A0A286YCW1_MOUSE | Cell cycle checkpoint protein RAD17 | Rad17 | 229 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 16 | T → E in CAA09868 (PubMed:10480350).Curated | 1 | |
Sequence conflicti | 86 | D → N in AAH66855 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 214 | D → V in AAH66855 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 397 | R → K in AAH66855 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 444 | P → S in AAH66855 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 580 | Q → P in CAA09868 (PubMed:10480350).Curated | 1 | |
Sequence conflicti | 616 – 619 | PHSG → AQR in CAA09868 (PubMed:10480350).Curated | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF085737 mRNA Translation: AAC36335.1 AJ011923 mRNA Translation: CAA09868.1 BC066855 mRNA Translation: AAH66855.1 |
CCDSi | CCDS26733.1 |
RefSeqi | NP_001037836.1, NM_001044371.2 NP_001269940.1, NM_001283011.1 NP_035363.2, NM_011233.3 |
Genome annotation databases
Ensembli | ENSMUST00000022136; ENSMUSP00000022136; ENSMUSG00000021635 ENSMUST00000177848; ENSMUSP00000136292; ENSMUSG00000021635 |
GeneIDi | 19356 |
KEGGi | mmu:19356 |
UCSCi | uc007rrc.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF085737 mRNA Translation: AAC36335.1 AJ011923 mRNA Translation: CAA09868.1 BC066855 mRNA Translation: AAH66855.1 |
CCDSi | CCDS26733.1 |
RefSeqi | NP_001037836.1, NM_001044371.2 NP_001269940.1, NM_001283011.1 NP_035363.2, NM_011233.3 |
3D structure databases
SMRi | Q6NXW6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202559, 1 interactor |
DIPi | DIP-59315N |
IntActi | Q6NXW6, 2 interactors |
STRINGi | 10090.ENSMUSP00000022136 |
PTM databases
iPTMneti | Q6NXW6 |
PhosphoSitePlusi | Q6NXW6 |
Proteomic databases
EPDi | Q6NXW6 |
MaxQBi | Q6NXW6 |
PaxDbi | Q6NXW6 |
PRIDEi | Q6NXW6 |
Protocols and materials databases
Antibodypediai | 1389, 621 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000022136; ENSMUSP00000022136; ENSMUSG00000021635 ENSMUST00000177848; ENSMUSP00000136292; ENSMUSG00000021635 |
GeneIDi | 19356 |
KEGGi | mmu:19356 |
UCSCi | uc007rrc.2, mouse |
Organism-specific databases
CTDi | 5884 |
MGIi | MGI:1333807, Rad17 |
Phylogenomic databases
eggNOGi | KOG1970, Eukaryota |
GeneTreei | ENSGT00440000039046 |
HOGENOMi | CLU_018598_0_0_1 |
InParanoidi | Q6NXW6 |
OMAi | EPWVDRY |
OrthoDBi | 674169at2759 |
PhylomeDBi | Q6NXW6 |
Enzyme and pathway databases
Reactomei | R-MMU-176187, Activation of ATR in response to replication stress R-MMU-5685938, HDR through Single Strand Annealing (SSA) R-MMU-5693607, Processing of DNA double-strand break ends R-MMU-5693616, Presynaptic phase of homologous DNA pairing and strand exchange R-MMU-6804756, Regulation of TP53 Activity through Phosphorylation R-MMU-69473, G2/M DNA damage checkpoint |
Miscellaneous databases
BioGRID-ORCSi | 19356, 9 hits in 19 CRISPR screens |
ChiTaRSi | Rad17, mouse |
PROi | PR:Q6NXW6 |
RNActi | Q6NXW6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021635, Expressed in cleaving embryo and 295 other tissues |
Genevisiblei | Q6NXW6, MM |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR004582, Checkpoint_prot_Rad17_Rad24 IPR027417, P-loop_NTPase IPR018324, Rad17/Rad24_fun/met |
PANTHERi | PTHR12172, PTHR12172, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00602, rad24, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAD17_MOUSE | |
Accessioni | Q6NXW6Primary (citable) accession number: Q6NXW6 Secondary accession number(s): O88934, O89024 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2005 |
Last sequence update: | March 29, 2005 | |
Last modified: | February 10, 2021 | |
This is version 135 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families