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Protein

Inositol polyphosphate 5-phosphatase OCRL-1

Gene

Ocrl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate. Also converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate. May function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes. Involved in primary cilia assembly (By similarity).By similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.

GO - Molecular functioni

GO - Biological processi

  • cilium assembly Source: MGI
  • in utero embryonic development Source: MGI
  • phosphatidylinositol dephosphorylation Source: MGI
  • regulation of GTPase activity Source: MGI
  • signal transduction Source: InterPro

Keywordsi

Molecular functionHydrolase
Biological processCilium biogenesis/degradation

Enzyme and pathway databases

ReactomeiR-MMU-1660499 Synthesis of PIPs at the plasma membrane
R-MMU-1660514 Synthesis of PIPs at the Golgi membrane
R-MMU-1855183 Synthesis of IP2, IP, and Ins in the cytosol
R-MMU-1855204 Synthesis of IP3 and IP4 in the cytosol
R-MMU-194840 Rho GTPase cycle
R-MMU-432722 Golgi Associated Vesicle Biogenesis
R-MMU-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol polyphosphate 5-phosphatase OCRL-1 (EC:3.1.3.36)
Gene namesi
Name:Ocrl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:109589 Ocrl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Coated pit, Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004215641 – 900Inositol polyphosphate 5-phosphatase OCRL-1Add BLAST900

Proteomic databases

PaxDbiQ6NVF0
PeptideAtlasiQ6NVF0
PRIDEiQ6NVF0

PTM databases

iPTMnetiQ6NVF0
PhosphoSitePlusiQ6NVF0

Expressioni

Gene expression databases

BgeeiENSMUSG00000001173
ExpressionAtlasiQ6NVF0 baseline and differential
GenevisibleiQ6NVF0 MM

Interactioni

Subunit structurei

Interacts with APPL1, PHETA1/SES1 and PHETA2/SES2; APPL1-binding and PHETA1-binding are mutually exclusive (By similarity). Interacts with clathrin heavy chain. Interacts with several Rab GTPases, at least RAB1B, RAB5A, RAB6A, RAB8A and RAB31; these interactions may play a dual role in targeting OCRL to the specific membranes and stimulating the phosphatase activitye (PubMed:25869668). Interaction with RAB8A modulates OCRL recruitment to cilia. Interacts with RAB31 (By similarity). Interacts with INPP5F (PubMed:25869668).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

CORUMiQ6NVF0
STRINGi10090.ENSMUSP00000001202

Structurei

3D structure databases

ProteinModelPortaliQ6NVF0
SMRiQ6NVF0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 119PHAdd BLAST119
Domaini720 – 900Rho-GAPPROSITE-ProRule annotationAdd BLAST181

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni236 – 5625-phosphataseBy similarityAdd BLAST327
Regioni563 – 677ASHBy similarityAdd BLAST115

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi73 – 77Clathrin box 15
Motifi701 – 705Clathrin box 25

Domaini

The ASH (ASPM-SPD2-Hydin) and RhoGAP (Rho GTPase activating) domains form a single folding module. The ASH domain has an immunoglobulin-like fold, the Rho-GAP domain lacks the catalytic arginine and is catalytically inactive. The ASH-RhoGAP module regulates the majority of the protein-protein interactions currently described. The ASH domain mediates association with membrane-targeting Rab GTPases. The Rho-GAP domain interacts with the endocytic adapter APPL1, which is then displaced by PHETA1 and PHETA2 as endosomes mature (By similarity).By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0565 Eukaryota
COG5411 LUCA
GeneTreeiENSGT00760000119075
HOGENOMiHOG000008071
HOVERGENiHBG000070
InParanoidiQ6NVF0
KOiK01099
OMAiASNSGCK
OrthoDBiEOG091G02KE
PhylomeDBiQ6NVF0
TreeFamiTF317034

Family and domain databases

CDDicd09093 INPP5c_INPP5B, 1 hit
cd13382 PH_OCRL1, 1 hit
Gene3Di1.10.555.10, 1 hit
2.60.40.10, 1 hit
3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
IPR013783 Ig-like_fold
IPR000300 IPPc
IPR037793 OCRL1/INPP5B_INPP5c
IPR037787 OCRL1_PH
IPR031995 OCRL_clath-bd
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
PF16726 OCRL_clath_bd, 1 hit
PF00620 RhoGAP, 1 hit
SMARTiView protein in SMART
SM00128 IPPc, 1 hit
SM00324 RhoGAP, 1 hit
SUPFAMiSSF48350 SSF48350, 2 hits
SSF56219 SSF56219, 1 hit
PROSITEiView protein in PROSITE
PS50238 RHOGAP, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6NVF0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPRLPIGAQ PLAMVAGLEM KGPLREPCVL TLARRNGQYE LIIQLHGKEQ
60 70 80 90 100
HVQDIIPINS HFRCVQEAEE TLLIDIASNS GCKIRVQGDW TRERHFEIPD
110 120 130 140 150
EERCLKFLSE VLEAQEAQSQ LLVPEQKDSS SWYQKLDTMD KPAYSGLLGF
160 170 180 190 200
EDNFSSLDLD KKMNTQNPRS GSHREPPPPP SSSTRMLSRE KEASNKEQPK
210 220 230 240 250
VTNTMRKLFV PNTQTGQREG LIKHILTKRE KEYVNIQSFR FFVGTWNVNG
260 270 280 290 300
QSPDSSLEPW LDCDPNPPDI YCIGFQELDL STEAFFYFES VKEQEWSLAV
310 320 330 340 350
ERGLPSKAKY RKVQLVRLVG MMLLIFARKD QCQYIRDVAT ETTGTGIMGK
360 370 380 390 400
MGNKGGVAVR FVFHNTTFCI VNSHLAAHVE EFERRNQDYK DICARMTFSV
410 420 430 440 450
PNQTVPQVNI MKHDVVIWLG DLNYRLCMPD ASEVKSLINK NELHKLLKFD
460 470 480 490 500
QLNIQRTQKK AFADFNEGEI NFVPTYKYDS KTDRWDSSGK CRVPAWCDRI
510 520 530 540 550
LWRGINVNQL HYRSHMELKT SDHKPVSALF HIGVKVVDER RYRKVFEDIV
560 570 580 590 600
RIMDRMENDF LPSLELSRRE FFFENVKFRQ LQKEKFQISN NGQVPCHFSF
610 620 630 640 650
IPKLNDSQYC KPWLRAEPFE GYLEPNETLD ISLDVYVSKD SVTILNSGED
660 670 680 690 700
KIEDILVLHL DRGKDYFLTI GGNYLPSCFG TSLEALCRMK RPIREVPVTK
710 720 730 740 750
LIDLEEDSYL EKEKSLLQMV PLDEGTSERP LQVPKEIWLL VDHLFKYACH
760 770 780 790 800
QEDLFQTPGM QEELQQIIDC LDTSIPETIP GNNHSVAEAL LIFLEALPEP
810 820 830 840 850
VICYELYQRC LDSAHDPRIC KQVISQLPRC HRNVFRYLMA FLRELLKFSD
860 870 880 890 900
YNNINTNMIA TLFSSLLLRP PPNLMTRQTP NDRQHAIQFL LVFLLGNEED
Length:900
Mass (Da):104,285
Last modified:July 5, 2004 - v1
Checksum:iF8C0985161F60696
GO
Isoform 2 (identifier: Q6NVF0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     713-741: EKSLLQMVPLDEGTSERPLQVPKEIWLLV → VIQSIGGNEGFLVLD
     742-900: Missing.

Note: No experimental confirmation available.
Show »
Length:727
Mass (Da):84,012
Checksum:i8E7C1377CE09A9C8
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_045749713 – 741EKSLL…IWLLV → VIQSIGGNEGFLVLD in isoform 2. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_045750742 – 900Missing in isoform 2. 1 PublicationAdd BLAST159

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK047906 mRNA Translation: BAC33188.1
AK041963 mRNA Translation: BAE20614.1
AL714010 Genomic DNA No translation available.
BC068146 mRNA Translation: AAH68146.1
CCDSiCCDS40957.1 [Q6NVF0-1]
RefSeqiNP_796189.2, NM_177215.3 [Q6NVF0-1]
UniGeneiMm.210343

Genome annotation databases

EnsembliENSMUST00000001202; ENSMUSP00000001202; ENSMUSG00000001173 [Q6NVF0-1]
ENSMUST00000115020; ENSMUSP00000110672; ENSMUSG00000001173 [Q6NVF0-2]
GeneIDi320634
KEGGimmu:320634
UCSCiuc009tbr.1 mouse [Q6NVF0-1]
uc009tbs.1 mouse [Q6NVF0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiOCRL_MOUSE
AccessioniPrimary (citable) accession number: Q6NVF0
Secondary accession number(s): Q8BXC9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: July 5, 2004
Last modified: June 20, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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