UniProtKB - Q6NU94 (TRMB_XENLA)
Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
mettl1
Organism
Xenopus laevis (African clawed frog)
Status
Functioni
Methyltransferase that mediates the formation of N7-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA. Also acts as a methyltransferase for a subset of internal N7-methylguanine in mRNAs. Internal N7-methylguanine methylation of mRNAs regulates translation. Also methylates a specific subset of miRNAs.UniRule annotation
Catalytic activityi
- guanosine46 in tRNA + S-adenosyl-L-methionine = N7-methylguanosine46 in tRNA + S-adenosyl-L-homocysteineUniRule annotationEC:2.1.1.33UniRule annotation
- a guanosine in mRNA + S-adenosyl-L-methionine = an N7-methylguanosine in mRNA + S-adenosyl-L-homocysteineUniRule annotationThis reaction proceeds in the forwardUniRule annotation direction.
- a guanosine in miRNA + S-adenosyl-L-methionine = an N7-methylguanosine in miRNA + S-adenosyl-L-homocysteineUniRule annotationThis reaction proceeds in the forwardUniRule annotation direction.
: N(7)-methylguanine-tRNA biosynthesis Pathwayi
This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotationView all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 86 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 162 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 | |
Active sitei | 165 | UniRule annotation | 1 |
GO - Molecular functioni
- tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB
- tRNA binding Source: UniProtKB-UniRule
GO - Biological processi
- tRNA modification Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase, tRNA-binding |
Biological process | tRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
UniPathwayi | UPA00989 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)Alternative name(s): Methyltransferase-like protein 1UniRule annotation mRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.-UniRule annotation) miRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.-UniRule annotation) tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation tRNA(m7G46)-methyltransferaseUniRule annotation |
Gene namesi | Name:mettl1 |
Organismi | Xenopus laevis (African clawed frog) |
Taxonomic identifieri | 8355 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Organism-specific databases
Xenbasei | XB-GENE-6252283, mettl1.L |
Subcellular locationi
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000370559 | 1 – 273 | tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST | 273 |
Interactioni
Subunit structurei
Forms a complex with wdr4.
UniRule annotationFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 109 – 110 | S-adenosyl-L-methionine bindingUniRule annotation | 2 | |
Regioni | 142 – 143 | S-adenosyl-L-methionine bindingUniRule annotation | 2 | |
Regioni | 240 – 242 | S-adenosyl-L-methionine bindingUniRule annotation | 3 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation
Phylogenomic databases
OMAi | KWRIINQ |
Family and domain databases
HAMAPi | MF_03055, tRNA_methyltr_TrmB_euk, 1 hit |
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR025763, Trm8_euk IPR003358, tRNA_(Gua-N-7)_MeTrfase_Trmb |
PANTHERi | PTHR23417, PTHR23417, 1 hit |
Pfami | View protein in Pfam PF02390, Methyltransf_4, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00091, TIGR00091, 1 hit |
PROSITEi | View protein in PROSITE PS51625, SAM_MT_TRMB, 1 hit |
i Sequence
Sequence statusi: Complete.
Q6NU94-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADTQAMASD RGVAVTLPQK RYYRQRAHSN PMADHTFQYP VKPEVMDWSE
60 70 80 90 100
YYPEFFKPLV PDCAHDDAKD LQERKEQHQV EFADVGCGYG GLLVALSPLF
110 120 130 140 150
PNTLMLGLEI RVKVSDYVQD RIKSLRASHL GQYQNIACIR SNAMKYLPNF
160 170 180 190 200
FKKGQLSKMF FLFPDPHFKK TKHKWRIISP TLLAEYSYAL RVGGMVYTIT
210 220 230 240 250
DVEEVHEWMV KHFTEHPLFE RVEKEELVSD IIVDKLGTST EEGKKVQRNK
260 270
GQNFLAVFRR IENRTFIQRD SQQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC068703 mRNA Translation: AAH68703.1 |
RefSeqi | NP_001084693.1, NM_001091224.1 |
Genome annotation databases
GeneIDi | 414654 |
KEGGi | xla:414654 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC068703 mRNA Translation: AAH68703.1 |
RefSeqi | NP_001084693.1, NM_001091224.1 |
3D structure databases
SMRi | Q6NU94 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 414654 |
KEGGi | xla:414654 |
Organism-specific databases
CTDi | 414654 |
Xenbasei | XB-GENE-6252283, mettl1.L |
Phylogenomic databases
OMAi | KWRIINQ |
Enzyme and pathway databases
UniPathwayi | UPA00989 |
Family and domain databases
HAMAPi | MF_03055, tRNA_methyltr_TrmB_euk, 1 hit |
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR025763, Trm8_euk IPR003358, tRNA_(Gua-N-7)_MeTrfase_Trmb |
PANTHERi | PTHR23417, PTHR23417, 1 hit |
Pfami | View protein in Pfam PF02390, Methyltransf_4, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00091, TIGR00091, 1 hit |
PROSITEi | View protein in PROSITE PS51625, SAM_MT_TRMB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRMB_XENLA | |
Accessioni | Q6NU94Primary (citable) accession number: Q6NU94 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 5, 2009 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 87 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families