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Protein

E3 ubiquitin-protein ligase RNFT1

Gene

rnft1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation. Protects cells from ER stress-induced apoptosis.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri356 – 394RING-typePROSITE-ProRule annotationAdd BLAST39

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNFT1Curated (EC:2.3.2.27By similarity)
Alternative name(s):
RING finger and transmembrane domain-containing protein 1
Gene namesi
Name:rnft1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-984040 rnft1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei146 – 166HelicalSequence analysisAdd BLAST21
Transmembranei184 – 204HelicalSequence analysisAdd BLAST21
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
Transmembranei237 – 257HelicalSequence analysisAdd BLAST21
Transmembranei265 – 287HelicalSequence analysisAdd BLAST23
Transmembranei302 – 322HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003206381 – 416E3 ubiquitin-protein ligase RNFT1Add BLAST416

Proteomic databases

PRIDEiQ6NTV1

Structurei

3D structure databases

ProteinModelPortaliQ6NTV1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni349 – 400Required for ubiquitin ligase activity and for protection against ER stress-induced cell deathBy similarityAdd BLAST52

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri356 – 394RING-typePROSITE-ProRule annotationAdd BLAST39

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

HOVERGENiHBG056546
KOiK22379

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF13639 zf-RING_2, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q6NTV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHRPVHERQ SSTESKNLKE TTQLIMQSSS DHTHHQLGSN DSPSACMSLP
60 70 80 90 100
VPQLSAEGSC TVGDVTVDLS SQDSQHVARS NSRRVRPSTH GRSPSRHGHT
110 120 130 140 150
HSHDASGPED ANDDSREQSN SISEVFHLYK WLEKSFPYIL IFSAKLVVQH
160 170 180 190 200
ITGISVGIGL LTTFLYANKC IVNQVFLRDK CSKLQCLWVL VFLLFSSFLL
210 220 230 240 250
YYTFSSQALY YSLVFMNPSL GPLHFFDALW VVGITDFIGK FFFMGLKCII
260 270 280 290 300
LLVPSFVMSH KSKGYWYMAL EELAQCYCTL VSTPVWFRYL IDYGNLDSGA
310 320 330 340 350
EWHFGILLAL LYLILKILII FGQRKTSSDC LRLFLSQPNY GTTATKRQCS
360 370 380 390 400
EADGMCAICQ AEFTKPIALI CQHVFCEECI SSWFNKEKTC PLCRTLISNH
410
SHKWKDGATS LQLRIF
Length:416
Mass (Da):47,122
Last modified:February 26, 2008 - v2
Checksum:i299BE3822FFBAB17
GO

Sequence cautioni

The sequence AAH77617 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86R → G in AAH68853 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC068853 mRNA Translation: AAH68853.1
BC077617 mRNA Translation: AAH77617.1 Different initiation.
RefSeqiNP_001084648.1, NM_001091179.1
XP_018100454.1, XM_018244965.1
UniGeneiXl.34003

Genome annotation databases

GeneIDi414607
KEGGixla:414607

Similar proteinsi

Entry informationi

Entry nameiRNFT1_XENLA
AccessioniPrimary (citable) accession number: Q6NTV1
Secondary accession number(s): Q6DDF3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: May 23, 2018
This is version 60 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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