UniProtKB - Q6NPT7 (HRD1B_ARATH)
Protein
ERAD-associated E3 ubiquitin-protein ligase HRD1B
Gene
HRD1B
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Probable component of the HRD1 ubiquitin ligase complex that mediates the rapid degradation of misfolded endoplasmic reticulum (ER) proteins, a process called ER-associated degradation (ERAD). Targets the misfolded LRR receptor kinase BRI1. Functions redundantly with HRD3A.1 Publication
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.Curated EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.CuratedView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 292 – 330 | RING-type; atypicalPROSITE-ProRule annotationAdd BLAST | 39 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin protein ligase activity Source: GO_Central
GO - Biological processi
- ubiquitin-dependent ERAD pathway Source: TAIR
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Protein family/group databases
TCDBi | 3.A.16.1.5, the endoplasmic reticular retrotranslocon (er-rt) family |
Names & Taxonomyi
Protein namesi | Recommended name: ERAD-associated E3 ubiquitin-protein ligase HRD1BCurated (EC:2.3.2.27Curated)Short name: AtHrd1B1 Publication Alternative name(s): RING-type E3 ubiquitin transferase HRD1BCurated |
Gene namesi | Name:HRD1B1 Publication Ordered Locus Names:At1g65040Imported ORF Names:F16G16.4Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G65040 |
TAIRi | locus:2014993, AT1G65040 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane Curated; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 3 | CytoplasmicCurated | 3 | |
Transmembranei | 4 – 24 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 25 – 40 | LumenalCuratedAdd BLAST | 16 | |
Transmembranei | 41 – 61 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 62 – 98 | CytoplasmicCuratedAdd BLAST | 37 | |
Transmembranei | 99 – 119 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 120 – 140 | LumenalCuratedAdd BLAST | 21 | |
Transmembranei | 141 – 161 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 162 – 170 | CytoplasmicCurated | 9 | |
Transmembranei | 171 – 191 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 192 – 225 | LumenalCuratedAdd BLAST | 34 | |
Transmembranei | 226 – 246 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 247 – 460 | CytoplasmicCuratedAdd BLAST | 214 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000431271 | 1 – 460 | ERAD-associated E3 ubiquitin-protein ligase HRD1BAdd BLAST | 460 |
Proteomic databases
PaxDbi | Q6NPT7 |
PRIDEi | Q6NPT7 |
ProteomicsDBi | 232175 [Q6NPT7-1] |
Expressioni
Gene expression databases
ExpressionAtlasi | Q6NPT7, baseline and differential |
Genevisiblei | Q6NPT7, AT |
Family & Domainsi
Sequence similaritiesi
Belongs to the HRD1 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 292 – 330 | RING-type; atypicalPROSITE-ProRule annotationAdd BLAST | 39 |
Keywords - Domaini
Transmembrane, Transmembrane helix, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0802, Eukaryota |
HOGENOMi | CLU_009169_4_2_1 |
InParanoidi | Q6NPT7 |
OMAi | EHECVIC |
OrthoDBi | 897451at2759 |
PhylomeDBi | Q6NPT7 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q6NPT7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MIQLKVYAGL STLATLVVIY HAFSSRGQFY PATVYLSTSK INLVVLLNMG
60 70 80 90 100
LVLMLSLWNL VKIVFLGSLR EAEVERLNEQ AWRELMEILF AITIFRQDFS
110 120 130 140 150
VGFISLVVTL LLIKGLHWMA QKRVEYIETT PSVTLLSHVR IVSFMVFLLI
160 170 180 190 200
LDCLLTYSSI QQLIQSRKAS MSVFFTFEYM ILATTTVSII VKYAFYVTDM
210 220 230 240 250
LKEGQWEGKP VYTFYLELVR DLLHLSMYLC FFLMIFMNYG LPLHLIRELY
260 270 280 290 300
ETFRNFKIRV TDYLRYRKIT SNMNDRFPDA TPEELSSNDA TCIICREEMT
310 320 330 340 350
SAKKLVCGHL FHVHCLRSWL ERQNTCPTCR ALVVPAENAT STASGNRGPH
360 370 380 390 400
QESLQQGTGT SSSDGQGSSV SAAASENMSR HEARFQAAAS AASIYGRSIV
410 420 430 440 450
YPSSANTLVW SQGNSLLPQT EVEAQRRFLE SQIEVLTNQL RLLEKPTTVD
460
TKGKSVADTA
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB3H675 | B3H675_ARATH | RING/U-box superfamily protein | Hrd1B AtHrd1B, homolog of yeast Hrd1, At1g65040, F16G16.3, F16G16_3 | 389 | Annotation score: | ||
Q27GM7 | Q27GM7_ARATH | RING/U-box superfamily protein | Hrd1B AtHrd1B, homolog of yeast Hrd1, At1g65040, F16G16.3, F16G16_3 | 281 | Annotation score: | ||
A0A1P8ASL9 | A0A1P8ASL9_ARATH | RING/U-box superfamily protein | Hrd1B AtHrd1B, homolog of yeast Hrd1, At1g65040, F16G16.3, F16G16_3 | 319 | Annotation score: | ||
A0A1P8ASM3 | A0A1P8ASM3_ARATH | RING/U-box superfamily protein | Hrd1B AtHrd1B, homolog of yeast Hrd1, At1g65040, F16G16.3, F16G16_3 | 437 | Annotation score: | ||
A0A1P8ASN3 | A0A1P8ASN3_ARATH | RING/U-box superfamily protein | Hrd1B AtHrd1B, homolog of yeast Hrd1, At1g65040, F16G16.3, F16G16_3 | 259 | Annotation score: |
Sequence cautioni
The sequence AAF06038 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB189470 mRNA Translation: BAD42325.1 AC009360 Genomic DNA Translation: AAF06038.1 Sequence problems. CP002684 Genomic DNA Translation: AEE34319.1 BT010730 mRNA Translation: AAR23700.1 AK228264 mRNA Translation: BAF00212.1 |
PIRi | B96674 |
RefSeqi | NP_849843.3, NM_179512.4 [Q6NPT7-1] |
Genome annotation databases
EnsemblPlantsi | AT1G65040.2; AT1G65040.2; AT1G65040 [Q6NPT7-1] |
GeneIDi | 842812 |
Gramenei | AT1G65040.2; AT1G65040.2; AT1G65040 [Q6NPT7-1] |
KEGGi | ath:AT1G65040 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB189470 mRNA Translation: BAD42325.1 AC009360 Genomic DNA Translation: AAF06038.1 Sequence problems. CP002684 Genomic DNA Translation: AEE34319.1 BT010730 mRNA Translation: AAR23700.1 AK228264 mRNA Translation: BAF00212.1 |
PIRi | B96674 |
RefSeqi | NP_849843.3, NM_179512.4 [Q6NPT7-1] |
3D structure databases
SMRi | Q6NPT7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT1G65040.2 |
Protein family/group databases
TCDBi | 3.A.16.1.5, the endoplasmic reticular retrotranslocon (er-rt) family |
Proteomic databases
PaxDbi | Q6NPT7 |
PRIDEi | Q6NPT7 |
ProteomicsDBi | 232175 [Q6NPT7-1] |
Genome annotation databases
EnsemblPlantsi | AT1G65040.2; AT1G65040.2; AT1G65040 [Q6NPT7-1] |
GeneIDi | 842812 |
Gramenei | AT1G65040.2; AT1G65040.2; AT1G65040 [Q6NPT7-1] |
KEGGi | ath:AT1G65040 |
Organism-specific databases
Araporti | AT1G65040 |
TAIRi | locus:2014993, AT1G65040 |
Phylogenomic databases
eggNOGi | KOG0802, Eukaryota |
HOGENOMi | CLU_009169_4_2_1 |
InParanoidi | Q6NPT7 |
OMAi | EHECVIC |
OrthoDBi | 897451at2759 |
PhylomeDBi | Q6NPT7 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
PROi | PR:Q6NPT7 |
Gene expression databases
ExpressionAtlasi | Q6NPT7, baseline and differential |
Genevisiblei | Q6NPT7, AT |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HRD1B_ARATH | |
Accessioni | Q6NPT7Primary (citable) accession number: Q6NPT7 Secondary accession number(s): Q3ECI3, Q9SS55 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 26, 2014 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families