We will be switching to the new UniProt website on Tuesday, June 28. Please explore and share your feedback.
Take me to UniProt BETA
UniProtKB - Q6NPM8 (HIS7_ARATH)
Protein
Bifunctional phosphatase IMPL2, chloroplastic
Gene
HISN7
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Phosphatase required for histidine production. Acts also on L-galactose 1-phosphate (L-Gal 1-P), D-myoinositol 3-phosphate (D-Ins 3-P) and D-myoinositol 1-phosphate (D-Ins 1-P).
3 PublicationsMiscellaneous
No redundancy with IMPL1 or VTC4.
Catalytic activityi
Cofactori
Mg2+By similarity
: L-histidine biosynthesis Pathwayi
This protein is involved in step 8 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate. This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.
Pathwayi: myo-inositol biosynthesis
This protein is involved in step 2 of the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate. This subpathway is part of the pathway myo-inositol biosynthesis, which is itself part of Polyol metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate, the pathway myo-inositol biosynthesis and in Polyol metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 147 | Magnesium 1By similarity | 1 | |
Binding sitei | 147 | SubstrateBy similarity | 1 | |
Metal bindingi | 165 | Magnesium 1By similarity | 1 | |
Metal bindingi | 165 | Magnesium 2By similarity | 1 | |
Metal bindingi | 168 | Magnesium 2By similarity | 1 | |
Binding sitei | 282 | SubstrateBy similarity | 1 | |
Metal bindingi | 289 | Magnesium 2By similarity | 1 | |
Binding sitei | 289 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- histidinol-phosphatase activity Source: UniProtKB
- inositol monophosphate 1-phosphatase activity Source: UniProtKB-EC
- inositol monophosphate 3-phosphatase activity Source: UniProtKB-EC
- inositol monophosphate 4-phosphatase activity Source: UniProtKB-EC
- inositol monophosphate phosphatase activity Source: UniProtKB
- L-galactose-1-phosphate phosphatase activity Source: RHEA
- metal ion binding Source: UniProtKB-KW
- phosphoric ester hydrolase activity Source: GO_Central
GO - Biological processi
- histidine biosynthetic process Source: UniProtKB
- inositol biosynthetic process Source: UniProtKB-UniPathway
- inositol phosphate dephosphorylation Source: InterPro
Keywordsi
Molecular function | Hydrolase |
Biological process | Amino-acid biosynthesis, Histidine biosynthesis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | ARA:AT4G39120-MONOMER |
BRENDAi | 3.1.3.15, 399 3.1.3.25, 399 |
UniPathwayi | UPA00031;UER00013 UPA00823;UER00788 |
Names & Taxonomyi
Protein namesi | Recommended name: Bifunctional phosphatase IMPL2, chloroplasticAlternative name(s): |
Gene namesi | Name:HISN7 Synonyms:IMPL2 Ordered Locus Names:At4g39120 ORF Names:T22F8.20 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G39120 |
Subcellular locationi
Chloroplast
- chloroplast 2 Publications
Chloroplast
- chloroplast Source: UniProtKB
Keywords - Cellular componenti
Chloroplast, PlastidPathology & Biotechi
Disruption phenotypei
Embryo lethality.3 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 195 | G → R: Loss of histidinol-phosphatase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 61 | ChloroplastSequence analysisAdd BLAST | 61 | |
ChainiPRO_0000383678 | 62 – 346 | Bifunctional phosphatase IMPL2, chloroplasticAdd BLAST | 285 |
Proteomic databases
PaxDbi | Q6NPM8 |
PRIDEi | Q6NPM8 |
ProteomicsDBi | 230246 |
Expressioni
Tissue specificityi
Ubiquitous. High expression in roots. Expressed in pistil and seed endosperm.2 Publications
Developmental stagei
Expressed at all developmental stages. Detected in globular to heart stage embryos.2 Publications
Gene expression databases
ExpressionAtlasi | Q6NPM8, baseline and differential |
Genevisiblei | Q6NPM8, AT |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 167 – 170 | Substrate bindingBy similarity | 4 | |
Regioni | 263 – 265 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the inositol monophosphatase superfamily.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2951, Eukaryota |
HOGENOMi | CLU_044118_4_1_1 |
InParanoidi | Q6NPM8 |
OMAi | WRTRAYG |
OrthoDBi | 1096950at2759 |
PhylomeDBi | Q6NPM8 |
Family and domain databases
InterProi | View protein in InterPro IPR011809, His_9_proposed IPR020583, Inositol_monoP_metal-BS IPR000760, Inositol_monophosphatase-like |
Pfami | View protein in Pfam PF00459, Inositol_P, 1 hit |
PRINTSi | PR00377, IMPHPHTASES |
TIGRFAMsi | TIGR02067, his_9_HisN, 1 hit |
PROSITEi | View protein in PROSITE PS00629, IMP_1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q6NPM8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLAQSHFFSK SFDLIPPQSP ALRSANPSLR ISSSYSNSRL SFLSSSAIAV
60 70 80 90 100
PVSRRRFCLT MASNSKRPNI SNESPSELSD TELDRFAAVG NALADASGEV
110 120 130 140 150
IRKYFRKKFD IVDKDDMSPV TIADQMAEEA MVSIIFQNLP SHAIYGEEKG
160 170 180 190 200
WRCKEESADY VWVLDPIDGT KSFITGKPVF GTLIALLYKG KPILGLIDQP
210 220 230 240 250
ILKERWIGMN GRRTKLNGED ISTRSCPKLS QAYLYTTSPH LFSEEAEKAY
260 270 280 290 300
SRVRDKVKVP LYGCDCYAYA LLASGFVDLV IESGLKPYDF LALVPVIEGA
310 320 330 340
GGTITDWTGK RFLWEASSSA VATSFNVVAA GDSDIHQQAL ESLEWH
Sequence cautioni
The sequence ANM67312 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence BAC43548 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence CAB43627 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence CAB80575 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL050351 Genomic DNA Translation: CAB43627.1 Different initiation. AL161594 Genomic DNA Translation: CAB80575.1 Different initiation. CP002687 Genomic DNA Translation: AEE87019.2 CP002687 Genomic DNA Translation: ANM67312.1 Different initiation. BT010891 mRNA Translation: AAR24669.1 AK175533 mRNA Translation: BAD43296.1 AK176630 mRNA Translation: BAD44393.1 AK220687 mRNA Translation: BAD93756.1 AK118971 mRNA Translation: BAC43548.1 Different initiation. |
PIRi | T08560 |
RefSeqi | NP_001320164.1, NM_001342523.1 NP_195623.3, NM_120072.5 |
Genome annotation databases
EnsemblPlantsi | AT4G39120.1; AT4G39120.1; AT4G39120 |
GeneIDi | 830067 |
Gramenei | AT4G39120.1; AT4G39120.1; AT4G39120 |
KEGGi | ath:AT4G39120 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL050351 Genomic DNA Translation: CAB43627.1 Different initiation. AL161594 Genomic DNA Translation: CAB80575.1 Different initiation. CP002687 Genomic DNA Translation: AEE87019.2 CP002687 Genomic DNA Translation: ANM67312.1 Different initiation. BT010891 mRNA Translation: AAR24669.1 AK175533 mRNA Translation: BAD43296.1 AK176630 mRNA Translation: BAD44393.1 AK220687 mRNA Translation: BAD93756.1 AK118971 mRNA Translation: BAC43548.1 Different initiation. |
PIRi | T08560 |
RefSeqi | NP_001320164.1, NM_001342523.1 NP_195623.3, NM_120072.5 |
3D structure databases
AlphaFoldDBi | Q6NPM8 |
SMRi | Q6NPM8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT4G39120.1 |
Proteomic databases
PaxDbi | Q6NPM8 |
PRIDEi | Q6NPM8 |
ProteomicsDBi | 230246 |
Genome annotation databases
EnsemblPlantsi | AT4G39120.1; AT4G39120.1; AT4G39120 |
GeneIDi | 830067 |
Gramenei | AT4G39120.1; AT4G39120.1; AT4G39120 |
KEGGi | ath:AT4G39120 |
Organism-specific databases
Araporti | AT4G39120 |
Phylogenomic databases
eggNOGi | KOG2951, Eukaryota |
HOGENOMi | CLU_044118_4_1_1 |
InParanoidi | Q6NPM8 |
OMAi | WRTRAYG |
OrthoDBi | 1096950at2759 |
PhylomeDBi | Q6NPM8 |
Enzyme and pathway databases
UniPathwayi | UPA00031;UER00013 UPA00823;UER00788 |
BioCyci | ARA:AT4G39120-MONOMER |
BRENDAi | 3.1.3.15, 399 3.1.3.25, 399 |
Miscellaneous databases
PROi | PR:Q6NPM8 |
Gene expression databases
ExpressionAtlasi | Q6NPM8, baseline and differential |
Genevisiblei | Q6NPM8, AT |
Family and domain databases
InterProi | View protein in InterPro IPR011809, His_9_proposed IPR020583, Inositol_monoP_metal-BS IPR000760, Inositol_monophosphatase-like |
Pfami | View protein in Pfam PF00459, Inositol_P, 1 hit |
PRINTSi | PR00377, IMPHPHTASES |
TIGRFAMsi | TIGR02067, his_9_HisN, 1 hit |
PROSITEi | View protein in PROSITE PS00629, IMP_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HIS7_ARATH | |
Accessioni | Q6NPM8Primary (citable) accession number: Q6NPM8 Secondary accession number(s): A0A1P8B6U8 Q9T021 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 22, 2009 |
Last sequence update: | July 5, 2004 | |
Last modified: | May 25, 2022 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families