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UniProtKB - Q6NH63 (ACNA_CORDI)
Protein
Aconitate hydratase A
Gene
acn
Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Functioni
Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and probably via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. Could catalyze the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate. The apo form of AcnA functions as a RNA-binding regulatory protein.
By similarityCatalytic activityi
Cofactori
[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity
: tricarboxylic acid cycle Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.By similarity This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.
Pathwayi: propanoate degradation
This protein is involved in the pathway propanoate degradation, which is part of Organic acid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway propanoate degradation and in Organic acid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 473 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 539 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 542 | Iron-sulfur (4Fe-4S)By similarity | 1 |
GO - Molecular functioni
- 2-methylisocitrate dehydratase activity Source: UniProtKB
- 4 iron, 4 sulfur cluster binding Source: UniProtKB
- aconitate hydratase activity Source: UniProtKB
- citrate dehydratase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- mRNA 3'-UTR binding Source: UniProtKB
- mRNA binding Source: UniProtKB
GO - Biological processi
- propionate metabolic process, methylcitrate cycle Source: UniProtKB
- tricarboxylic acid cycle Source: UniProtKB
Keywordsi
Molecular function | Lyase, RNA-binding |
Biological process | Tricarboxylic acid cycle |
Ligand | Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | CDIP257309:G1GT3-1300-MONOMER |
UniPathwayi | UPA00223;UER00718 UPA00946 |
Names & Taxonomyi
Protein namesi | Recommended name: Aconitate hydratase ABy similarity (EC:4.2.1.3By similarity)Short name: ACNBy similarity Short name: AconitaseBy similarity Alternative name(s): (2R,3S)-2-methylisocitrate dehydrataseBy similarity (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydrataseBy similarity Iron-responsive protein-likeBy similarity Short name: IRP-likeBy similarity Probable 2-methyl-cis-aconitate hydrataseBy similarity (EC:4.2.1.99By similarity) RNA-binding proteinBy similarity |
Gene namesi | Name:acn Synonyms:acnA Ordered Locus Names:DIP1283 |
Organismi | Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis) |
Taxonomic identifieri | 257309 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Corynebacteriaceae › Corynebacterium › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076658 | 1 – 934 | Aconitate hydratase AAdd BLAST | 934 |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 257309.DIP1283 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 398 – 454 | DisorderedSequence analysisAdd BLAST | 57 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 403 – 417 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Belongs to the aconitase/IPM isomerase family.Curated
Phylogenomic databases
HOGENOMi | CLU_013476_2_1_11 |
OMAi | NGGIMQY |
OrthoDBi | 363064at2 |
Family and domain databases
CDDi | cd01580, AcnA_IRP_Swivel, 1 hit |
Gene3Di | 3.20.19.10, 1 hit 3.30.499.10, 2 hits |
InterProi | View protein in InterPro IPR044137, AcnA_IRP_Swivel IPR015931, Acnase/IPM_dHydase_lsu_aba_1/3 IPR001030, Acoase/IPM_deHydtase_lsu_aba IPR015928, Aconitase/3IPM_dehydase_swvl IPR006249, Aconitase/IRP2 IPR018136, Aconitase_4Fe-4S_BS IPR036008, Aconitase_4Fe-4S_dom IPR000573, AconitaseA/IPMdHydase_ssu_swvl |
PANTHERi | PTHR11670, PTHR11670, 1 hit |
Pfami | View protein in Pfam PF00330, Aconitase, 1 hit PF00694, Aconitase_C, 1 hit |
PRINTSi | PR00415, ACONITASE |
SUPFAMi | SSF53732, SSF53732, 1 hit |
TIGRFAMsi | TIGR01341, aconitase_1, 1 hit |
PROSITEi | View protein in PROSITE PS00450, ACONITASE_1, 1 hit PS01244, ACONITASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q6NH63-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTESKNSFNA KQTLEVGDKS YDYFALSAVK GMEKLPYSLK VLGENLLRTE
60 70 80 90 100
DGANITADHI NAIANWDPSA EPSIEIQFTP ARVLMQDFTG VPCVVDLATM
110 120 130 140 150
REAVKTLGGD PDKVNPLNPA EMVIDHSVII EAFGSTLALA KNVEIEYERN
160 170 180 190 200
EERYQFLRWG SKAFSNFRVV PPGTGIVHQV NIENLARVVF DNNGLAYPDT
210 220 230 240 250
CIGTDSHTTM ENGLGILGWG VGGIEAEAAM LGQPVSMLIP RVVGFKLTGE
260 270 280 290 300
IPAGVTATDV VLTITEMLRE HGVVQKFVEF YGNGVKSIPL ANRATIGNMS
310 320 330 340 350
PEFGSTCAIF PIDEETVKYM HLTGRSEEQV ALVEAYAKAQ GMWLEQDAPE
360 370 380 390 400
AEYSEYLELD LSTVVPSIAG PKRPQDRILL TEAKEQFRKD LPDYCSAEPV
410 420 430 440 450
DESLPAKRMD SEGAVQKEGE DVAGYNSSRA GHGESAAEGA AGRQSNPVVV
460 470 480 490 500
SSPNGGEYTL DHGMVAIASI TSCTNTSNPS VMIGAGLIAR KAAAKGLKAK
510 520 530 540 550
PWVKTICAPG SQVVDGYYKR ADLWKDLEAL GFYLSGFGCT TCIGNSGPLP
560 570 580 590 600
EEISAAINEN DLTATAVLSG NRNFEGRISP DVKMNYLVSP IMVIAYAIAG
610 620 630 640 650
TMDFDFETQP LGQDIDGNDV FLKDIWPSTE EIEETIAGAI SRELYEADYA
660 670 680 690 700
DVFKGDEQWQ NLPTPEGKTF DWDEKSTYIR KAPYFDGMTM EPAPVSDIKG
710 720 730 740 750
ARVLAKLGDS VTTDHISPAS SIKPGTPAAQ YLDENGVARN DYNSLGSRRG
760 770 780 790 800
NHEVMMRGTF ANIRLQNQLV DIAGGYTRDF TKNGEQAFIF DACQNYKAAG
810 820 830 840 850
IPLVVIAGKE YGTGSSRDWA AKGTNLLGVK AVITESFERI HRSNLIGMGV
860 870 880 890 900
IPLQFPAGES HASLGLDGTE TFDIEGIEEL NNGVTPKTVH VTATKESGDQ
910 920 930
VEFDAVVRID TPGEADYYRN GGILQYVLRN MINA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX248357 Genomic DNA Translation: CAE49810.1 |
RefSeqi | WP_041627895.1, NC_002935.2 |
Genome annotation databases
EnsemblBacteriai | CAE49810; CAE49810; DIP1283 |
KEGGi | cdi:DIP1283 |
PATRICi | fig|257309.4.peg.1262 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX248357 Genomic DNA Translation: CAE49810.1 |
RefSeqi | WP_041627895.1, NC_002935.2 |
3D structure databases
AlphaFoldDBi | Q6NH63 |
SMRi | Q6NH63 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 257309.DIP1283 |
Genome annotation databases
EnsemblBacteriai | CAE49810; CAE49810; DIP1283 |
KEGGi | cdi:DIP1283 |
PATRICi | fig|257309.4.peg.1262 |
Phylogenomic databases
HOGENOMi | CLU_013476_2_1_11 |
OMAi | NGGIMQY |
OrthoDBi | 363064at2 |
Enzyme and pathway databases
UniPathwayi | UPA00223;UER00718 UPA00946 |
BioCyci | CDIP257309:G1GT3-1300-MONOMER |
Family and domain databases
CDDi | cd01580, AcnA_IRP_Swivel, 1 hit |
Gene3Di | 3.20.19.10, 1 hit 3.30.499.10, 2 hits |
InterProi | View protein in InterPro IPR044137, AcnA_IRP_Swivel IPR015931, Acnase/IPM_dHydase_lsu_aba_1/3 IPR001030, Acoase/IPM_deHydtase_lsu_aba IPR015928, Aconitase/3IPM_dehydase_swvl IPR006249, Aconitase/IRP2 IPR018136, Aconitase_4Fe-4S_BS IPR036008, Aconitase_4Fe-4S_dom IPR000573, AconitaseA/IPMdHydase_ssu_swvl |
PANTHERi | PTHR11670, PTHR11670, 1 hit |
Pfami | View protein in Pfam PF00330, Aconitase, 1 hit PF00694, Aconitase_C, 1 hit |
PRINTSi | PR00415, ACONITASE |
SUPFAMi | SSF53732, SSF53732, 1 hit |
TIGRFAMsi | TIGR01341, aconitase_1, 1 hit |
PROSITEi | View protein in PROSITE PS00450, ACONITASE_1, 1 hit PS01244, ACONITASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACNA_CORDI | |
Accessioni | Q6NH63Primary (citable) accession number: Q6NH63 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 7, 2006 |
Last sequence update: | July 5, 2004 | |
Last modified: | May 25, 2022 | |
This is version 106 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families