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Protein

5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase

Gene

purP

Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates.UniRule annotation

Catalytic activityi

ATP + formate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = ADP + phosphate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase (purP)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei27SubstrateUniRule annotation1
Binding sitei94SubstrateUniRule annotation1
Binding sitei230ATPUniRule annotation1
Binding sitei258SubstrateUniRule annotation1
Metal bindingi297Magnesium or manganeseUniRule annotation1
Metal bindingi310Magnesium or manganeseUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi146 – 208ATPUniRule annotationAdd BLAST63

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMAR267377:MMP_RS07080-MONOMER
UniPathwayi
UPA00074;UER00134

Names & Taxonomyi

Protein namesi
Recommended name:
5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetaseUniRule annotation (EC:6.3.4.23UniRule annotation)
Alternative name(s):
5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligaseUniRule annotation
Gene namesi
Name:purPUniRule annotation
Ordered Locus Names:MMP1373
OrganismiMethanococcus maripaludis (strain S2 / LL)
Taxonomic identifieri267377 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomada groupMethanococciMethanococcalesMethanococcaceaeMethanococcus
Proteomesi
  • UP000000590 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001480261 – 3615-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetaseAdd BLAST361

Interactioni

Protein-protein interaction databases

STRINGi267377.MMP1373

Structurei

3D structure databases

ProteinModelPortaliQ6LXH8
SMRiQ6LXH8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini116 – 348ATP-graspUniRule annotationAdd BLAST233

Sequence similaritiesi

Belongs to the phosphohexose mutase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04346 Archaea
COG1759 LUCA
HOGENOMiHOG000228474
KOiK06863
OMAiKFPGARG
OrthoDBiPOG093Z04BP

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_01163 IMP_biosynth_PurP, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR023656 IMP_biosynth_PurP
IPR009720 IMP_biosynth_PurP_C
IPR010672 IMP_biosynth_PurP_N
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR38147 PTHR38147, 1 hit
PfamiView protein in Pfam
PF06849 DUF1246, 1 hit
PF06973 DUF1297, 1 hit
PIRSFiPIRSF004602 ATPgrasp_PurP, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit

Sequencei

Sequence statusi: Complete.

Q6LXH8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIPKEEIMGI FEKYNKDEVT IVTVGSHTSL HILKGAKLEG FSTAVITTKD
60 70 80 90 100
RAIPYKRFGV ADKFIYVDQF SDISKEEIQQ QLRDMNAIIV PHGSFIAYCG
110 120 130 140 150
LDNVEDTFKV PMFGNRAILR WEAERDLEGQ LLGGSGLRIP KKYGGPDEID
160 170 180 190 200
GPVMVKFPGA RGGRGYFPCS SVEEFWRKID EFKAKGVLTE DDVSKAHIEE
210 220 230 240 250
YVVGANYCIH YFYSPLKDQV ELMGIDRRYE SSIDGLVRVP AKDQLELDID
260 270 280 290 300
PSYVITGNFP VVIRESLLPQ VFDMGDKLVA KAKEEVNPGM LGPFCLQSLC
310 320 330 340 350
NENLELVVFE MSARVDGGTN TFMNGSPYSC LYTGEPLSMG QRIAKEIKLA
360
LELDMIDKVI S
Length:361
Mass (Da):40,413
Last modified:July 5, 2004 - v1
Checksum:iD83E36F00F7AFCE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950229 Genomic DNA Translation: CAF30929.1
RefSeqiWP_011171317.1, NC_005791.1

Genome annotation databases

EnsemblBacteriaiCAF30929; CAF30929; MMP1373
GeneIDi2762237
KEGGimmp:MMP1373
PATRICifig|267377.15.peg.1408

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950229 Genomic DNA Translation: CAF30929.1
RefSeqiWP_011171317.1, NC_005791.1

3D structure databases

ProteinModelPortaliQ6LXH8
SMRiQ6LXH8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267377.MMP1373

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAF30929; CAF30929; MMP1373
GeneIDi2762237
KEGGimmp:MMP1373
PATRICifig|267377.15.peg.1408

Phylogenomic databases

eggNOGiarCOG04346 Archaea
COG1759 LUCA
HOGENOMiHOG000228474
KOiK06863
OMAiKFPGARG
OrthoDBiPOG093Z04BP

Enzyme and pathway databases

UniPathwayi
UPA00074;UER00134

BioCyciMMAR267377:MMP_RS07080-MONOMER

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_01163 IMP_biosynth_PurP, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR023656 IMP_biosynth_PurP
IPR009720 IMP_biosynth_PurP_C
IPR010672 IMP_biosynth_PurP_N
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR38147 PTHR38147, 1 hit
PfamiView protein in Pfam
PF06849 DUF1246, 1 hit
PF06973 DUF1297, 1 hit
PIRSFiPIRSF004602 ATPgrasp_PurP, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPURP_METMP
AccessioniPrimary (citable) accession number: Q6LXH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 5, 2004
Last modified: October 10, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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