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Protein

Alanine dehydrogenase

Gene

ald

Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible reductive amination of pyruvate to L-alanine. This enzyme is a key factor in the assimilation of L-alanine as an energy source through the tricarboxylic acid cycle.1 Publication

Catalytic activityi

L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH.

Pathwayi: L-alanine degradation via dehydrogenase pathway

This protein is involved in step 1 of the subpathway that synthesizes NH(3) and pyruvate from L-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine dehydrogenase (ald)
This subpathway is part of the pathway L-alanine degradation via dehydrogenase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NH(3) and pyruvate from L-alanine, the pathway L-alanine degradation via dehydrogenase pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15SubstrateBy similarity1
Binding sitei75SubstrateBy similarity1
Active sitei96Proton donor/acceptorSequence analysis1
Binding sitei134NADBy similarity1
Binding sitei198NADBy similarity1
Binding sitei220NADBy similarity1
Active sitei270Proton donor/acceptorBy similarity1
Binding sitei280NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 179NADBy similarity2
Nucleotide bindingi239 – 240NADBy similarity2
Nucleotide bindingi267 – 270NADBy similarity4
Nucleotide bindingi299 – 302NADBy similarity4

GO - Molecular functioni

  • alanine dehydrogenase activity Source: UniProtKB
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMAR267377:MMP_RS07775-MONOMER
UniPathwayi
UPA00527;UER00585

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine dehydrogenase (EC:1.4.1.1)
Gene namesi
Name:ald
Ordered Locus Names:MMP1513
OrganismiMethanococcus maripaludis (strain S2 / LL)
Taxonomic identifieri267377 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomada groupMethanococciMethanococcalesMethanococcaceaeMethanococcus
Proteomesi
  • UP000000590 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No growth on either stereoisoform of alanine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004154431 – 373Alanine dehydrogenaseAdd BLAST373

Interactioni

Subunit structurei

Homohexamer. Trimer of dimer (By similarity).By similarity

Protein-protein interaction databases

STRINGi267377.MMP1513

Structurei

3D structure databases

ProteinModelPortaliQ6LX40
SMRiQ6LX40
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AlaDH/PNT family.Curated

Phylogenomic databases

eggNOGiarCOG06678 Archaea
COG0686 LUCA
HOGENOMiHOG000022120
KOiK00259
OMAiCFETSKA
OrthoDBiPOG093Z04PA

Family and domain databases

CDDicd05305 L-AlaDH, 1 hit
InterProiView protein in InterPro
IPR008141 Ala_DH
IPR008143 Ala_DH/PNT_CS2
IPR007886 AlaDH/PNT_N
IPR007698 AlaDH/PNT_NAD(H)-bd
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR42795 PTHR42795, 1 hit
PfamiView protein in Pfam
PF01262 AlaDh_PNT_C, 1 hit
PF05222 AlaDh_PNT_N, 1 hit
PIRSFiPIRSF000183 Alanine_dh, 1 hit
SMARTiView protein in SMART
SM01002 AlaDh_PNT_C, 1 hit
SM01003 AlaDh_PNT_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00518 alaDH, 1 hit
PROSITEiView protein in PROSITE
PS00837 ALADH_PNT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q6LX40-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLIGVPKEIK NNENRVAITP AGVEAFVLAG HKVYIEKGAG LGSAITDEEY
60 70 80 90 100
VAAGAEILAT AKEVFDTAEM IIKVKEPLKS EFELFKEGQI LFTYLHLAPE
110 120 130 140 150
PQLTKALLDK KITGIAYETV QLADRSLPLL TPMSEIAGRM SIQLGAQYLE
160 170 180 190 200
KQEGGKGVLL GGVPGVEPAE VVIVGGGIVG TNAAKIAVGM GAKVTILDVN
210 220 230 240 250
TRRLAYLDDI FGNKVTTLMS NSFNIAKAVK KADLLVGCVL IPGAKAPKLV
260 270 280 290 300
SEEMVKSMQP GSVIVDVAID QGGSIETIDR ITTHDNPTYE KFGVIHYAVA
310 320 330 340 350
NIPGAVARTS TYALTNDTLP YALQIANKGY KQAVIENPAL LKGLNTLNGK
360 370
VTYEAVAKAH DLPYTPAEKA LNE
Length:373
Mass (Da):39,654
Last modified:July 5, 2004 - v1
Checksum:i6B0C51C099774302
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950229 Genomic DNA Translation: CAF31069.1
RefSeqiWP_011171457.1, NC_005791.1

Genome annotation databases

EnsemblBacteriaiCAF31069; CAF31069; MMP1513
GeneIDi2762318
KEGGimmp:MMP1513
PATRICifig|267377.15.peg.1550

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950229 Genomic DNA Translation: CAF31069.1
RefSeqiWP_011171457.1, NC_005791.1

3D structure databases

ProteinModelPortaliQ6LX40
SMRiQ6LX40
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267377.MMP1513

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAF31069; CAF31069; MMP1513
GeneIDi2762318
KEGGimmp:MMP1513
PATRICifig|267377.15.peg.1550

Phylogenomic databases

eggNOGiarCOG06678 Archaea
COG0686 LUCA
HOGENOMiHOG000022120
KOiK00259
OMAiCFETSKA
OrthoDBiPOG093Z04PA

Enzyme and pathway databases

UniPathwayi
UPA00527;UER00585

BioCyciMMAR267377:MMP_RS07775-MONOMER

Family and domain databases

CDDicd05305 L-AlaDH, 1 hit
InterProiView protein in InterPro
IPR008141 Ala_DH
IPR008143 Ala_DH/PNT_CS2
IPR007886 AlaDH/PNT_N
IPR007698 AlaDH/PNT_NAD(H)-bd
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR42795 PTHR42795, 1 hit
PfamiView protein in Pfam
PF01262 AlaDh_PNT_C, 1 hit
PF05222 AlaDh_PNT_N, 1 hit
PIRSFiPIRSF000183 Alanine_dh, 1 hit
SMARTiView protein in SMART
SM01002 AlaDh_PNT_C, 1 hit
SM01003 AlaDh_PNT_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00518 alaDH, 1 hit
PROSITEiView protein in PROSITE
PS00837 ALADH_PNT_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDHA_METMP
AccessioniPrimary (citable) accession number: Q6LX40
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: July 5, 2004
Last modified: October 10, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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