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Protein

Endoglycoceramidase

Gene
N/A
Organism
Hydra vulgaris (Hydra) (Hydra attenuata)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolysis of the glycosidic linkage between oligosaccharides and ceramides of glycosphingolipids, optimal substrates appear to be the glycosphingolipids with a gangliotetraose structure.1 Publication

Catalytic activityi

Oligoglycosylglucosyl-(1<->1)-ceramide + H2O = ceramide + oligoglycosylglucose.1 Publication

Enzyme regulationi

Cu2+, zinc, manganese, calcium, magnesium and EDTA have no significant effects on enzyme activity. Enzyme requires presence of detergents such as Triton X-100 and Lubrol PX for the hydrolysis of glycosphingolipids. Taurodeoxycholate strongly inhibits the enzyme activity.1 Publication

pH dependencei

Optimum pH is 3.0-4.0. Highly stable at acidic pH.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei230Proton donorSequence analysis1

GO - Molecular functioni

  • endoglycosylceramidase activity Source: UniProtKB

GO - Biological processi

  • cellulose catabolic process Source: UniProtKB-KW
  • glycosphingolipid catabolic process Source: UniProtKB

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18387

Protein family/group databases

CAZyiGH5 Glycoside Hydrolase Family 5

Names & Taxonomyi

Protein namesi
Recommended name:
EndoglycoceramidaseImported (EC:3.2.1.123)
Short name:
EGCase1 Publication
Alternative name(s):
Glycosphingolipid-specific enzyme1 Publication
Short name:
GSL-specific enzyme1 Publication
OrganismiHydra vulgaris (Hydra) (Hydra attenuata)
Taxonomic identifieri6087 [NCBI]
Taxonomic lineageiEukaryotaMetazoaCnidariaHydrozoaHydroidolinaAnthoathecataAplanulataHydridaeHydra

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000039038218 – 517EndoglycoceramidaseSequence analysisAdd BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi99N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi298N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi380N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi393N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed uniformly in digestive cells, tentacles and peduncle regions suggesting expression in the endoderm throughout the whole body (at protein level).1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ6L6S1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 5 (cellulase A) family.Sequence analysis

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.1180, 2 hits
InterProiView protein in InterPro
IPR001547 Glyco_hydro_5
IPR018087 Glyco_hydro_5_CS
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00150 Cellulase, 1 hit
SUPFAMiSSF51445 SSF51445, 2 hits
PROSITEiView protein in PROSITE
PS00659 GLYCOSYL_HYDROL_F5, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6L6S1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISVALIILF LAKVISGKSD DFISVNPETN MLIDGYGRER FFHGTNVVVK
60 70 80 90 100
HFPFHPETTG FNKDTFSEDD MKILQKFGLN SIRLGMMLPG YVPKREEYNE
110 120 130 140 150
TYIKVIQSIV TTAAKYGIYT LLDMHQDVFS PKFCVEGMPD WIVNTQGAKD
160 170 180 190 200
FPMPLHKPFN LDPKTGYPYP EDCAKFSWAD YYFTEAAGQA FQNLYDNVDG
210 220 230 240 250
LRDEWAQFWK KTADVFKEEP SVIGYELINE PFCGNVFKHP TLLIPGVADY
260 270 280 290 300
LNLQPTYDAL QKAIRQVDEE HNIFFEGVTW DFFEVGFTEV PGGKQYQNRS
310 320 330 340 350
VLSYHYYEPP DFSKKLNFEA RLLDLKRLKC GGFLTEMFTV GTDFNSMFEM
360 370 380 390 400
FDLCDKFKQS WHGWMYKSYG CIEQNLGCLN MSSPGKESIQ IANTSRTYPQ
410 420 430 440 450
AVAGRTQSYA FDIKTKVFTL VYETVGSCKS GRTIVYFNKN LHYPNGYRYE
460 470 480 490 500
INPNFKVTPS ENEYFLYLDE VNKVPNTVVT FKLFPLSFTD SEDIHPVTVM
510
GDKHLSENHN ENEKKKK
Length:517
Mass (Da):59,685
Last modified:July 5, 2004 - v1
Checksum:i9042532367D263F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB179748 mRNA Translation: BAD20464.1

Similar proteinsi

Entry informationi

Entry nameiEGCSE_HYDVU
AccessioniPrimary (citable) accession number: Q6L6S1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: July 5, 2004
Last modified: February 28, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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