UniProtKB - Q6J5K9 (ARMI_DROME)
Probable RNA helicase armi
armi
Functioni
Probable RNA helicase required for axial polarization of the oocyte during early and mid oogenesis. Plays a central role in RNA interference (RNAi) process, a process that mediates mRNA destruction of translational repression. Required for the assembly of the RISC complex, a complex required for target RNA destruction or repression. May be required in the RISC assembly to unwind miRNAs, in the production of single-stranded miRNA from the double-stranded miRNA, a key step in RISC formation. Required both for the translational control of oskar (osk) mRNA and cytoskeletal polarization in the oocyte. Required for somatic primary piRNA biogenesis.
3 PublicationsCatalytic activityi
- EC:3.6.4.13
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 723 – 730 | ATPSequence analysis | 8 |
GO - Molecular functioni
- 5'-3' RNA helicase activity Source: FlyBase
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: RHEA
- RNA binding Source: FlyBase
- RNA helicase activity Source: UniProtKB
GO - Biological processi
- dorsal appendage formation Source: FlyBase
- embryonic axis specification Source: UniProtKB
- germarium-derived egg chamber formation Source: FlyBase
- long-term memory Source: FlyBase
- microtubule organizing center organization Source: FlyBase
- negative regulation of DNA damage checkpoint Source: FlyBase
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
- oogenesis Source: FlyBase
- piRNA biosynthetic process Source: FlyBase
- pole plasm protein localization Source: FlyBase
- post-transcriptional gene silencing by RNA Source: GO_Central
- RNA interference Source: UniProtKB
- small RNA loading onto RISC Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, Helicase, Hydrolase |
Biological process | RNA-mediated gene silencing |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:armi ORF Names:CG11513 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0041164, armi |
VEuPathDBi | VectorBase:FBgn0041164 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Note: Component of Yb bodies, an electron dense structure containing Yb protein found in somatic cells of ovary and testis.
Cytosol
- cytosol Source: FlyBase
Mitochondrion
- mitochondrion Source: FlyBase
Other locations
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080708 | 1 – 1188 | Probable RNA helicase armiAdd BLAST | 1188 |
Proteomic databases
PaxDbi | Q6J5K9 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | FBgn0041164, Expressed in female gonad and 33 other tissues |
ExpressionAtlasi | Q6J5K9, baseline and differential |
Genevisiblei | Q6J5K9, DM |
Interactioni
Subunit structurei
Forms a complex with piwi and fs1Yb; this interaction is required for proper piRNA loading and nuclear localization of piwi. The interaction of piwi and fs1Yb is likely to occur via armi.
Binary interactionsi
Protein-protein interaction databases
BioGRIDi | 63920, 16 interactors |
IntActi | Q6J5K9, 7 interactors |
MINTi | Q6J5K9 |
STRINGi | 7227.FBpp0100102 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 862 – 865 | DEAG box | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1804, Eukaryota |
GeneTreei | ENSGT00940000165903 |
InParanoidi | Q6J5K9 |
OrthoDBi | 286011at2759 |
PhylomeDBi | Q6J5K9 |
Family and domain databases
CDDi | cd18808, SF1_C_Upf1, 1 hit |
Gene3Di | 3.40.50.300, 3 hits |
InterProi | View protein in InterPro IPR045055, DNA2/NAM7-like IPR041679, DNA2/NAM7-like_C IPR041677, DNA2/NAM7_AAA_11 IPR026122, MOV-10 IPR027417, P-loop_NTPase |
PANTHERi | PTHR10887, PTHR10887, 1 hit PTHR10887:SF419, PTHR10887:SF419, 1 hit |
Pfami | View protein in Pfam PF13086, AAA_11, 2 hits PF13087, AAA_12, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MFTYVSKFFT NPDRNREDIL ESLDRENSFL DQKLMEEKMD QQLKANPNEI
60 70 80 90 100
NGVLSNKIAE LTHGLSEMDV SKESSCTARK GVITSLDGDR GVIDKDVLFE
110 120 130 140 150
TKVAEDIILD LHVGCVVEYL TFTTGEAMRV VKVKSILEHS WEDTSQKEIE
160 170 180 190 200
KAVDNLKNEK PTFFNTETRS VLGLISQRLA SSIDVETEYG QLTVELDNIE
210 220 230 240 250
MNFIPTNGDR VRLECNIQLD DGFVDKQGEI LEVTKLFPTR IQEGEKCIVE
260 270 280 290 300
RVYVHMVVLG PETYILKTDL PTGTDLHLGD IVLADLIECQ YSKFTRRAIK
310 320 330 340 350
ITPLEKNFGA TKLTQQSSMG SSGSGKAVTV TGVNRFITAE LWQKESVSLK
360 370 380 390 400
LTNNLNRTLR LESITVCNDS ESQLSVVSPL ESKEISSGSE ITVTFEIHTQ
410 420 430 440 450
FLGEAIEKYV LNFDLLKVRR VFTVIVCKTK EEVAEAEKRM IAAEALMAPG
460 470 480 490 500
RNSQERSRFY ANQVWCNKVD VIPGQQIVTK RRFVALRLGC FEVPKELRQI
510 520 530 540 550
CLTSERRQEM IKAIEQHYSF LTEPLSIKTY MHRFRLLLHL EEIECFVNFR
560 570 580 590 600
NYDRDRAHFL RDGEFLTLQI ENLAERRPSL VIGDTLRVIN PWSDPDSQTT
610 620 630 640 650
KSYEGIIHKV LFDRILLKFH SSFQEKYNGE DYRLEFYFSR YSFRKQHHAI
660 670 680 690 700
SKIVGVMGED FLFPSKVTKR ENPQLDVYMK DDDMYLYDSK LEWYNQSLNS
710 720 730 740 750
IQKRAVFNIL RGEAENIPYV LFGPPGSGKT MTLIETLLQL VRNLPGARIL
760 770 780 790 800
VGTPSNSSAD LVTKRLIDSK ALLQGDFIRL VSYNQVEKDL IPPEIMSYCA
810 820 830 840 850
TSDVGAVGSC EDKMMVTESG LKLRCQAKFI GTHRITISTC TTLGNFLQLG
860 870 880 890 900
FPAGHFTHVL FDEAGQCTEP ETMVPIVMLT KKRSQVVLSG DPRQLQSIVT
910 920 930 940 950
SRIASKMGFS ISFLERLLER SPYRKDLQRF PESSGYNPLV LTKLLYNYRA
960 970 980 990 1000
LPSIMSIYSR LFYDDELIPV LSEKDSRESR LLSKLRCVFE SEKDIPQAHG
1010 1020 1030 1040 1050
TFFYGIIGEN RQNNDSPSWF NPQEVREVFL MTIALYRANV TADQIGIITP
1060 1070 1080 1090 1100
YQKQVKMLRS MFIGTDVVMP KIGSVEEFQG QERDIILIST VRSSEEILRM
1110 1120 1130 1140 1150
DARFSLGFVR CSKRLNVAVS RARAMMIIFG NPHLLAVDEC WRQLILFCVK
1160 1170 1180
NNAYFGCDLP QMVINQKDEV PVCLETFVPS LNTTDDLN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM9NDG7 | M9NDG7_DROME | RNA helicase | armi APC16, ARMI, Armi, Dmel\CG11513, CG11513 | 1,154 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 522 | T → K in AAT12000 (PubMed:15035984).Curated | 1 | |
Sequence conflicti | 527 | I → V in AAT12000 (PubMed:15035984).Curated | 1 | |
Sequence conflicti | 905 | S → L in AAT12000 (PubMed:15035984).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY598469 mRNA Translation: AAT12000.1 AE014296 Genomic DNA Translation: AAF47775.2 AE014296 Genomic DNA Translation: AAX52729.1 |
RefSeqi | NP_001014556.1, NM_001014556.3 NP_647816.2, NM_139559.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0100641; FBpp0100102; FBgn0041164 FBtr0309841; FBpp0301575; FBgn0041164 |
GeneIDi | 38427 |
KEGGi | dme:Dmel_CG11513 |
UCSCi | CG11513-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY598469 mRNA Translation: AAT12000.1 AE014296 Genomic DNA Translation: AAF47775.2 AE014296 Genomic DNA Translation: AAX52729.1 |
RefSeqi | NP_001014556.1, NM_001014556.3 NP_647816.2, NM_139559.4 |
3D structure databases
SMRi | Q6J5K9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 63920, 16 interactors |
IntActi | Q6J5K9, 7 interactors |
MINTi | Q6J5K9 |
STRINGi | 7227.FBpp0100102 |
Proteomic databases
PaxDbi | Q6J5K9 |
Genome annotation databases
EnsemblMetazoai | FBtr0100641; FBpp0100102; FBgn0041164 FBtr0309841; FBpp0301575; FBgn0041164 |
GeneIDi | 38427 |
KEGGi | dme:Dmel_CG11513 |
UCSCi | CG11513-RA, d. melanogaster |
Organism-specific databases
CTDi | 38427 |
FlyBasei | FBgn0041164, armi |
VEuPathDBi | VectorBase:FBgn0041164 |
Phylogenomic databases
eggNOGi | KOG1804, Eukaryota |
GeneTreei | ENSGT00940000165903 |
InParanoidi | Q6J5K9 |
OrthoDBi | 286011at2759 |
PhylomeDBi | Q6J5K9 |
Miscellaneous databases
BioGRID-ORCSi | 38427, 0 hits in 3 CRISPR screens |
GenomeRNAii | 38427 |
PROi | PR:Q6J5K9 |
Gene expression databases
Bgeei | FBgn0041164, Expressed in female gonad and 33 other tissues |
ExpressionAtlasi | Q6J5K9, baseline and differential |
Genevisiblei | Q6J5K9, DM |
Family and domain databases
CDDi | cd18808, SF1_C_Upf1, 1 hit |
Gene3Di | 3.40.50.300, 3 hits |
InterProi | View protein in InterPro IPR045055, DNA2/NAM7-like IPR041679, DNA2/NAM7-like_C IPR041677, DNA2/NAM7_AAA_11 IPR026122, MOV-10 IPR027417, P-loop_NTPase |
PANTHERi | PTHR10887, PTHR10887, 1 hit PTHR10887:SF419, PTHR10887:SF419, 1 hit |
Pfami | View protein in Pfam PF13086, AAA_11, 2 hits PF13087, AAA_12, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ARMI_DROME | |
Accessioni | Q6J5K9Primary (citable) accession number: Q6J5K9 Secondary accession number(s): Q59E56, Q9VZP4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | July 9, 2014 | |
Last modified: | February 23, 2022 | |
This is version 137 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families