UniProtKB - Q6ITZ3 (ML4_VISAL)
Protein
Beta-galactoside-specific lectin 4
Gene
N/A
Organism
Viscum album (European mistletoe)
Status
Functioni
The A chain is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits by removing adenine from position 4,324 of 28S rRNA. The B chain binds to cell receptors and probably facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (lectin activity). Inhibits growth of the human tumor cell line Molt4.By similarity2 Publications
Miscellaneous
Several isoforms exist.1 Publication
Catalytic activityi
- Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.By similarity EC:3.2.2.22
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 159 | 1 Publication | 1 |
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
- rRNA N-glycosylase activity Source: UniProtKB-EC
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- defense response Source: UniProtKB-KW
- negative regulation of translation Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protein synthesis inhibitor, Toxin |
Biological process | Plant defense |
Ligand | Lectin |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 |
UniLectini | Q6ITZ3 |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-galactoside-specific lectin 4Alternative name(s): Beta-galactoside-specific lectin IV Cleaved into the following 2 chains: Alternative name(s): Beta-galactoside-specific lectin IV chain A ML-4 A ML-IV A rRNA N-glycosidase Alternative name(s): Beta-galactoside-specific lectin IV chain B ML-4B ML-IV B |
Organismi | Viscum album (European mistletoe) |
Taxonomic identifieri | 3972 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › Santalales › Viscaceae › Viscum |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_5000093497 | 1 – 240 | Beta-galactoside-specific lectin 4 chain AAdd BLAST | 240 | |
PropeptideiPRO_0000284730 | 241 – 265 | Connecting peptideCuratedAdd BLAST | 25 | |
ChainiPRO_5000093498 | 266 – 520 | Beta-galactoside-specific lectin 4 chain BAdd BLAST | 255 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 107 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 240 ↔ 266 | Interchain (between A and B chains)PROSITE-ProRule annotation2 Publications | ||
Glycosylationi | 322 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 325 ↔ 342 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 357 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 397 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 413 ↔ 426 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 451 ↔ 467 | PROSITE-ProRule annotation1 Publication |
Keywords - PTMi
Disulfide bond, GlycoproteinPTM databases
iPTMneti | Q6ITZ3 |
Interactioni
Subunit structurei
Disulfide-linked dimer of A and B chains.
2 PublicationsStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q6ITZ3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q6ITZ3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 269 – 396 | Ricin B-type lectin 1PROSITE-ProRule annotationAdd BLAST | 128 | |
Domaini | 400 – 520 | Ricin B-type lectin 2PROSITE-ProRule annotationAdd BLAST | 121 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 284 – 286 | Galactose bindingBy similarity | 3 | |
Regioni | 494 – 496 | Galactose bindingBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
RepeatFamily and domain databases
CDDi | cd00161, RICIN, 2 hits |
Gene3Di | 3.40.420.10, 1 hit 4.10.470.10, 1 hit |
InterProi | View protein in InterPro IPR036041, Ribosome-inact_prot_sf IPR017989, Ribosome_inactivat_1/2 IPR001574, Ribosome_inactivat_prot IPR016138, Ribosome_inactivat_prot_sub1 IPR016139, Ribosome_inactivat_prot_sub2 IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
PANTHERi | PTHR33453, PTHR33453, 1 hit |
Pfami | View protein in Pfam PF00652, Ricin_B_lectin, 2 hits PF00161, RIP, 1 hit |
PRINTSi | PR00396, SHIGARICIN |
SMARTi | View protein in SMART SM00458, RICIN, 2 hits |
SUPFAMi | SSF50370, SSF50370, 2 hits SSF56371, SSF56371, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q6ITZ3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
YERLDLDVTS QTTGEEYFRF ITLLRDYVSS GSFSNEIPLL RQSGGGVEAA
60 70 80 90 100
RFVLVELTNE GGDSITAAID VTNLYVVAYQ AGSQSYFLSG PGTHLFTGTT
110 120 130 140 150
RSSLPFNGSY PDLEQYAGHR KQIPLGIDQL IQSVTALRFP GNTRTQARSI
160 170 180 190 200
LILIQMISEA ARFNPILWRA RQYINSGASF LPDVYMLELE TSWGQQSTQV
210 220 230 240 250
QQSTEGVFNN PIRLAIPGNF VTLTNVRDVI ASLAIMLFVC GERPSSSDVR
260 270 280 290 300
YWPLVIRPVI ADDVTCSASE PTVRIVGRNG MNVDVRDDDF HDGNQIQLWP
310 320 330 340 350
SKSNNDPNQL WTIKRDGTIR SNGSCLTTYG YTAGVYVMIF DCNTAVREAT
360 370 380 390 400
IWQIWGNGTI INPRSNLALA ASSGIKGTTL TVQTLDYTLG QGWLAGNDTA
410 420 430 440 450
PREVTIYGFN DLCMESNGGS VWVETCVSQQ NDRWALYGDG SIRPEQNQDQ
460 470 480 490 500
CLTSGRDSVA GINIVSCSGG SSGQRWVFTN EGAILNLKNG LAMDVANPGL
510 520
GQIIIYPATG KPNQMWLPVP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 5 | D → R AA sequence (PubMed:15001393).Curated | 1 | |
Sequence conflicti | 5 | D → R AA sequence (PubMed:1450445).Curated | 1 | |
Sequence conflicti | 7 | D → R AA sequence (PubMed:15001393).Curated | 1 | |
Sequence conflicti | 7 | D → R AA sequence (PubMed:1450445).Curated | 1 | |
Sequence conflicti | 10 | S → H AA sequence (PubMed:15001393).Curated | 1 | |
Sequence conflicti | 10 | S → H AA sequence (PubMed:1450445).Curated | 1 | |
Sequence conflicti | 29 | S → H AA sequence (PubMed:1450445).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY625281 mRNA Translation: AAT37532.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY625281 mRNA Translation: AAT37532.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1CE7 | X-ray | 2.70 | A | 1-240 | [»] | |
B | 266-519 | [»] | ||||
1PC8 | X-ray | 3.80 | A | 1-240 | [»] | |
B | 266-520 | [»] | ||||
1TFM | X-ray | 2.80 | A | 1-240 | [»] | |
B | 266-520 | [»] | ||||
1YF8 | X-ray | 2.80 | A | 1-240 | [»] | |
B | 266-520 | [»] | ||||
2MLL | X-ray | 2.70 | A | 1-240 | [»] | |
B | 266-519 | [»] | ||||
SMRi | Q6ITZ3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 |
UniLectini | Q6ITZ3 |
PTM databases
iPTMneti | Q6ITZ3 |
Miscellaneous databases
EvolutionaryTracei | Q6ITZ3 |
Family and domain databases
CDDi | cd00161, RICIN, 2 hits |
Gene3Di | 3.40.420.10, 1 hit 4.10.470.10, 1 hit |
InterProi | View protein in InterPro IPR036041, Ribosome-inact_prot_sf IPR017989, Ribosome_inactivat_1/2 IPR001574, Ribosome_inactivat_prot IPR016138, Ribosome_inactivat_prot_sub1 IPR016139, Ribosome_inactivat_prot_sub2 IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
PANTHERi | PTHR33453, PTHR33453, 1 hit |
Pfami | View protein in Pfam PF00652, Ricin_B_lectin, 2 hits PF00161, RIP, 1 hit |
PRINTSi | PR00396, SHIGARICIN |
SMARTi | View protein in SMART SM00458, RICIN, 2 hits |
SUPFAMi | SSF50370, SSF50370, 2 hits SSF56371, SSF56371, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ML4_VISAL | |
Accessioni | Q6ITZ3Primary (citable) accession number: Q6ITZ3 Secondary accession number(s): Q9S7D0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 17, 2007 |
Last sequence update: | July 5, 2004 | |
Last modified: | April 7, 2021 | |
This is version 71 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families