UniProtKB - Q6ITZ3 (ML4_VISAL)

BLAST

>sp|Q6ITZ3|ML4_VISAL Beta-galactoside-specific lectin 4 OS=Viscum album OX=3972 PE=1 SV=1
YERLDLDVTSQTTGEEYFRFITLLRDYVSSGSFSNEIPLLRQSGGGVEAARFVLVELTNE
GGDSITAAIDVTNLYVVAYQAGSQSYFLSGPGTHLFTGTTRSSLPFNGSYPDLEQYAGHR
KQIPLGIDQLIQSVTALRFPGNTRTQARSILILIQMISEAARFNPILWRARQYINSGASF
LPDVYMLELETSWGQQSTQVQQSTEGVFNNPIRLAIPGNFVTLTNVRDVIASLAIMLFVC
GERPSSSDVRYWPLVIRPVIADDVTCSASEPTVRIVGRNGMNVDVRDDDFHDGNQIQLWP
SKSNNDPNQLWTIKRDGTIRSNGSCLTTYGYTAGVYVMIFDCNTAVREATIWQIWGNGTI
INPRSNLALAASSGIKGTTLTVQTLDYTLGQGWLAGNDTAPREVTIYGFNDLCMESNGGS
VWVETCVSQQNDRWALYGDGSIRPEQNQDQCLTSGRDSVAGINIVSCSGGSSGQRWVFTN
EGAILNLKNGLAMDVANPGLGQIIIYPATGKPNQMWLPVP

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History
Entry version 64 (18 Jul 2018)
Sequence version 1 (05 Jul 2004)
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Protein

Beta-galactoside-specific lectin 4

Gene

N/A

Organism

Viscum album (European mistletoe)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

The A chain is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits by removing adenine from position 4,324 of 28S rRNA. The B chain binds to cell receptors and probably facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (lectin activity). Inhibits growth of the human tumor cell line Molt4.By similarity2 Publications

Miscellaneous

Several isoforms exist.1 Publication

Catalytic activityⁱ

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	159	1 Publication		1

GO - Molecular functionⁱ

carbohydrate binding Source: UniProtKB-KW
rRNA N-glycosylase activity Source: UniProtKB-EC
toxin activity Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

defense response Source: UniProtKB-KW
negative regulation of translation Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Protein synthesis inhibitor, Toxin
Biological process	Plant defense
Ligand	Lectin

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	CBM13 Carbohydrate-Binding Module Family 13
UniLectinⁱ	Q6ITZ3

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Beta-galactoside-specific lectin 4 Alternative name(s): Beta-galactoside-specific lectin IV Cleaved into the following 2 chains: Beta-galactoside-specific lectin 4 chain A (EC:3.2.2.22) Alternative name(s): Beta-galactoside-specific lectin IV chain A ML-4 A ML-IV A rRNA N-glycosidase Beta-galactoside-specific lectin 4 chain B Alternative name(s): Beta-galactoside-specific lectin IV chain B ML-4B ML-IV B
Organismⁱ	Viscum album (European mistletoe)
Taxonomic identifierⁱ	3972 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › Santalales › Viscaceae › Viscum

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
ChainⁱPRO_5000093497	1 – 240	Beta-galactoside-specific lectin 4 chain AAdd BLAST	240
PropeptideⁱPRO_0000284730	241 – 265	Connecting peptideCuratedAdd BLAST	25
ChainⁱPRO_5000093498	266 – 520	Beta-galactoside-specific lectin 4 chain BAdd BLAST	255

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	107	N-linked (GlcNAc...) asparagine1 Publication	1
Disulfide bondⁱ	240 ↔ 266	Interchain (between A and B chains)PROSITE-ProRule annotation2 Publications
Glycosylationⁱ	322	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	325 ↔ 342	PROSITE-ProRule annotation1 Publication
Glycosylationⁱ	357	N-linked (GlcNAc...) asparagine1 Publication	1
Glycosylationⁱ	397	N-linked (GlcNAc...) asparagine1 Publication	1
Disulfide bondⁱ	413 ↔ 426	PROSITE-ProRule annotation1 Publication
Disulfide bondⁱ	451 ↔ 467	PROSITE-ProRule annotation1 Publication

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

PTM databases

iPTMnetⁱ	Q6ITZ3

iPTMnetⁱ

Q6ITZ3

Interactionⁱ

Subunit structureⁱ

Disulfide-linked dimer of A and B chains.2 Publications

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	3 – 11	Combined sources	9
Helixⁱ	14 – 28	Combined sources	15
Beta strandⁱ	29 – 34	Combined sources	6
Beta strandⁱ	37 – 40	Combined sources	4
Beta strandⁱ	43 – 45	Combined sources	3
Beta strandⁱ	50 – 59	Combined sources	10
Beta strandⁱ	64 – 70	Combined sources	7
Turnⁱ	71 – 73	Combined sources	3
Beta strandⁱ	76 – 80	Combined sources	5
Beta strandⁱ	82 – 87	Combined sources	6
Beta strandⁱ	98 – 101	Combined sources	4
Helixⁱ	110 – 117	Combined sources	8
Helixⁱ	120 – 122	Combined sources	3
Beta strandⁱ	125 – 127	Combined sources	3
Helixⁱ	128 – 138	Combined sources	11
Helixⁱ	144 – 156	Combined sources	13
Helixⁱ	159 – 162	Combined sources	4
Helixⁱ	164 – 176	Combined sources	13
Helixⁱ	184 – 190	Combined sources	7
Helixⁱ	193 – 202	Combined sources	10
Beta strandⁱ	204 – 214	Combined sources	11
Beta strandⁱ	217 – 219	Combined sources	3
Beta strandⁱ	221 – 225	Combined sources	5
Helixⁱ	226 – 229	Combined sources	4
Turnⁱ	230 – 232	Combined sources	3
Beta strandⁱ	275 – 277	Combined sources	3
Helixⁱ	278 – 280	Combined sources	3
Beta strandⁱ	281 – 285	Combined sources	5
Helixⁱ	286 – 288	Combined sources	3
Beta strandⁱ	295 – 299	Combined sources	5
Helixⁱ	307 – 309	Combined sources	3
Beta strandⁱ	311 – 313	Combined sources	3
Beta strandⁱ	315 – 317	Combined sources	3
Beta strandⁱ	319 – 321	Combined sources	3
Beta strandⁱ	324 – 329	Combined sources	6
Beta strandⁱ	336 – 340	Combined sources	5
Turnⁱ	342 – 344	Combined sources	3
Helixⁱ	347 – 350	Combined sources	4
Beta strandⁱ	360 – 362	Combined sources	3
Turnⁱ	363 – 366	Combined sources	4
Beta strandⁱ	367 – 370	Combined sources	4
Beta strandⁱ	381 – 383	Combined sources	3
Helixⁱ	389 – 391	Combined sources	3
Beta strandⁱ	394 – 397	Combined sources	4
Beta strandⁱ	402 – 408	Combined sources	7
Helixⁱ	409 – 411	Combined sources	3
Beta strandⁱ	412 – 417	Combined sources	6
Beta strandⁱ	420 – 425	Combined sources	6
Beta strandⁱ	428 – 430	Combined sources	3
Beta strandⁱ	434 – 437	Combined sources	4
Turnⁱ	438 – 440	Combined sources	3
Beta strandⁱ	441 – 446	Combined sources	6
Beta strandⁱ	450 – 453	Combined sources	4
Beta strandⁱ	455 – 460	Combined sources	6
Beta strandⁱ	463 – 467	Combined sources	5
Helixⁱ	472 – 474	Combined sources	3
Beta strandⁱ	476 – 478	Combined sources	3
Beta strandⁱ	484 – 486	Combined sources	3
Turnⁱ	487 – 489	Combined sources	3
Beta strandⁱ	492 – 495	Combined sources	4
Helixⁱ	498 – 500	Combined sources	3
Beta strandⁱ	503 – 506	Combined sources	4
Helixⁱ	512 – 514	Combined sources	3
Beta strandⁱ	516 – 519	Combined sources	4

Show more details

3D structure databases

ProteinModelPortalⁱ	Q6ITZ3
SMRⁱ	Q6ITZ3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q6ITZ3

EvolutionaryTraceⁱ

Q6ITZ3

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	269 – 396	Ricin B-type lectin 1PROSITE-ProRule annotationAdd BLAST		128
Domainⁱ	400 – 520	Ricin B-type lectin 2PROSITE-ProRule annotationAdd BLAST		121

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	284 – 286	Galactose bindingBy similarity		3
Regionⁱ	494 – 496	Galactose bindingBy similarity		3

Sequence similaritiesⁱ

Belongs to the ribosome-inactivating protein family. Type 2 RIP subfamily.Sequence analysis

Keywords - Domainⁱ

Repeat

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00161 RICIN, 2 hits
Gene3Dⁱ	3.40.420.10, 1 hit 4.10.470.10, 1 hit
InterProⁱ	View protein in InterPro IPR036041 Ribosome-inact_prot_sf IPR017989 Ribosome_inactivat_1/2 IPR001574 Ribosome_inactivat_prot IPR016138 Ribosome_inactivat_prot_sub1 IPR016139 Ribosome_inactivat_prot_sub2 IPR035992 Ricin_B-like_lectins IPR000772 Ricin_B_lectin
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00652 Ricin_B_lectin, 2 hits PF00161 RIP, 1 hit
PRINTSⁱ	PR00396 SHIGARICIN
SMARTⁱ	View protein in SMART SM00458 RICIN, 2 hits
SUPFAMⁱ	SSF50370 SSF50370, 2 hits SSF56371 SSF56371, 1 hit
PROSITEⁱ	View protein in PROSITE PS50231 RICIN_B_LECTIN, 2 hits

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q6ITZ3-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
YERLDLDVTS QTTGEEYFRF ITLLRDYVSS GSFSNEIPLL RQSGGGVEAA 
        60         70         80         90        100
RFVLVELTNE GGDSITAAID VTNLYVVAYQ AGSQSYFLSG PGTHLFTGTT 
       110        120        130        140        150
RSSLPFNGSY PDLEQYAGHR KQIPLGIDQL IQSVTALRFP GNTRTQARSI 
       160        170        180        190        200
LILIQMISEA ARFNPILWRA RQYINSGASF LPDVYMLELE TSWGQQSTQV 
       210        220        230        240        250
QQSTEGVFNN PIRLAIPGNF VTLTNVRDVI ASLAIMLFVC GERPSSSDVR 
       260        270        280        290        300
YWPLVIRPVI ADDVTCSASE PTVRIVGRNG MNVDVRDDDF HDGNQIQLWP 
       310        320        330        340        350
SKSNNDPNQL WTIKRDGTIR SNGSCLTTYG YTAGVYVMIF DCNTAVREAT 
       360        370        380        390        400
IWQIWGNGTI INPRSNLALA ASSGIKGTTL TVQTLDYTLG QGWLAGNDTA 
       410        420        430        440        450
PREVTIYGFN DLCMESNGGS VWVETCVSQQ NDRWALYGDG SIRPEQNQDQ 
       460        470        480        490        500
CLTSGRDSVA GINIVSCSGG SSGQRWVFTN EGAILNLKNG LAMDVANPGL 
       510        520
GQIIIYPATG KPNQMWLPVP

Length:520

Mass (Da):56,957

Last modified:July 5, 2004 - v1

Checksum:ⁱ4F9561A0BD92A915

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	5	D → R AA sequence (PubMed:15001393).Curated	1
Sequence conflictⁱ	5	D → R AA sequence (PubMed:1450445).Curated	1
Sequence conflictⁱ	7	D → R AA sequence (PubMed:15001393).Curated	1
Sequence conflictⁱ	7	D → R AA sequence (PubMed:1450445).Curated	1
Sequence conflictⁱ	10	S → H AA sequence (PubMed:15001393).Curated	1
Sequence conflictⁱ	10	S → H AA sequence (PubMed:1450445).Curated	1
Sequence conflictⁱ	29	S → H AA sequence (PubMed:1450445).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY625281 mRNA Translation: AAT37532.1

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

AY625281 mRNA Translation: AAT37532.1

Protein	Similar proteins		Species	Score	Length	Source
Q6ITZ3	Beta-galactoside-specific lectin 1	)	VISAL		564	UniRef90_P81446
UPI00003B0EC6		VISAL		255
UPI00003B0EC5		VISAL		240

Protein	Similar proteins		Species	Score	Length	Source
Q6ITZ3	Beta-galactoside-specific lectin 1	)	VISAL		564	UniRef50_P81446
Beta-galactoside-specific lectin 2		VISAL		567
UPI00003B0EC6		VISAL		255
UPI00003B0EC5		VISAL		240

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY625281 mRNA Translation: AAT37532.1

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

AY625281 mRNA Translation: AAT37532.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1CE7	X-ray	2.70	A	1-240	[»]
				B	266-519	[»]
	1PC8	X-ray	3.80	A	1-240	[»]
				B	266-520	[»]
	1TFM	X-ray	2.80	A	1-240	[»]
				B	266-520	[»]
	1YF8	X-ray	2.80	A	1-240	[»]
				B	266-520	[»]
	2MLL	X-ray	2.70	A	1-240	[»]
				B	266-519	[»]
ProteinModelPortalⁱ	Q6ITZ3
SMRⁱ	Q6ITZ3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein family/group databases

CAZyⁱ	CBM13 Carbohydrate-Binding Module Family 13
UniLectinⁱ	Q6ITZ3

PTM databases

iPTMnetⁱ	Q6ITZ3

iPTMnetⁱ

Q6ITZ3

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q6ITZ3

EvolutionaryTraceⁱ

Q6ITZ3

Family and domain databases

CDDⁱ	cd00161 RICIN, 2 hits
Gene3Dⁱ	3.40.420.10, 1 hit 4.10.470.10, 1 hit
InterProⁱ	View protein in InterPro IPR036041 Ribosome-inact_prot_sf IPR017989 Ribosome_inactivat_1/2 IPR001574 Ribosome_inactivat_prot IPR016138 Ribosome_inactivat_prot_sub1 IPR016139 Ribosome_inactivat_prot_sub2 IPR035992 Ricin_B-like_lectins IPR000772 Ricin_B_lectin
Pfamⁱ	View protein in Pfam PF00652 Ricin_B_lectin, 2 hits PF00161 RIP, 1 hit
PRINTSⁱ	PR00396 SHIGARICIN
SMARTⁱ	View protein in SMART SM00458 RICIN, 2 hits
SUPFAMⁱ	SSF50370 SSF50370, 2 hits SSF56371 SSF56371, 1 hit
PROSITEⁱ	View protein in PROSITE PS50231 RICIN_B_LECTIN, 2 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ML4_VISAL
Accessionⁱ	Q6ITZ3Primary (citable) accession number: Q6ITZ3 Secondary accession number(s): Q9S7D0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
	Last sequence update:	July 5, 2004
	Last modified:	July 18, 2018
	This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

UniProtKB

UniParc

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Annotation systems

Supporting data

UniProtKB - Q6ITZ3 (ML4_VISAL)

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Beta-galactoside-specific lectin 4

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Family and domain databases

Experimental Info

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein family/group databases

PTM databases

Protocols and materials databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ