UniProtKB - Q6IQ49 (SDE2_HUMAN)
Replication stress response regulator SDE2
SDE2
Functioni
Involved in both DNA replication and cell cycle control (PubMed:27906959).
Unprocessed SDE2 interacts with PCNA via its PIP-box. The interaction with PCNA prevents monoubiquitination of the latter thereby inhibiting translesion DNA synthesis. The binding of SDE2 to PCNA also leads to processing of SDE2 by an unidentified deubiquitinating enzyme, cleaving off the N-terminal ubiquitin-like domain. The resulting mature SDE2 is degraded by the DCX(DTL) complex in a cell cycle- and DNA damage dependent manner (PubMed:27906959).
Binding of SDE2 to PCNA is necessary to counteract damage due to ultraviolet light induced replication stress. The complete degradation of SDE2 is necessary to allow S-phase progression (PubMed:27906959).
1 PublicationGO - Molecular functioni
- damaged DNA binding Source: UniProtKB
GO - Biological processi
- cell division Source: UniProtKB-KW
- cellular response to UV Source: UniProtKB
- DNA replication Source: UniProtKB-KW
- mitotic G1 DNA damage checkpoint signaling Source: UniProtKB
- protein processing Source: UniProtKB
- protein ubiquitination Source: UniProtKB
Keywordsi
Molecular function | DNA-binding |
Biological process | Cell cycle, Cell division, DNA replication, Mitosis |
Enzyme and pathway databases
PathwayCommonsi | Q6IQ49 |
SignaLinki | Q6IQ49 |
Names & Taxonomyi
Protein namesi | Recommended name: Replication stress response regulator SDE2Curated |
Gene namesi | Name:SDE2 Synonyms:C1orf55 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:26643, SDE2 |
neXtProti | NX_Q6IQ49 |
VEuPathDBi | HostDB:ENSG00000143751 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytosol
- cytosol Source: HPA
Golgi apparatus
- Golgi apparatus Source: HPA
Nucleus
- nuclear speck Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: HPA
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 47 – 48 | FF → AA: No binding to PCNA; no cleavage at Gly-77. 1 Publication | 2 | |
Mutagenesisi | 76 – 77 | GG → AA: No cleavage at Gly-77. 1 Publication | 2 |
Organism-specific databases
OpenTargetsi | ENSG00000143751 |
PharmGKBi | PA142672506 |
Miscellaneous databases
Pharosi | Q6IQ49, Tbio |
Genetic variation databases
BioMutai | SDE2 |
DMDMi | 74748828 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000442521 | 1 – 77 | UBL1 PublicationAdd BLAST | 77 | |
ChainiPRO_0000286084 | 78 – 451 | Replication stress response regulator SDE2Add BLAST | 374 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 266 | PhosphoserineBy similarity | 1 | |
Modified residuei | 274 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 278 | PhosphoserineCombined sources | 1 | |
Modified residuei | 319 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 326 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 77 – 78 | Cleavage1 Publication | 2 |
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q6IQ49 |
jPOSTi | Q6IQ49 |
MassIVEi | Q6IQ49 |
MaxQBi | Q6IQ49 |
PaxDbi | Q6IQ49 |
PeptideAtlasi | Q6IQ49 |
PRIDEi | Q6IQ49 |
ProteomicsDBi | 66484 [Q6IQ49-1] 66485 [Q6IQ49-2] 66486 [Q6IQ49-3] |
PTM databases
GlyGeni | Q6IQ49, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q6IQ49 |
MetOSitei | Q6IQ49 |
PhosphoSitePlusi | Q6IQ49 |
SwissPalmi | Q6IQ49 |
Expressioni
Gene expression databases
Bgeei | ENSG00000143751, Expressed in secondary oocyte and 221 other tissues |
Genevisiblei | Q6IQ49, HS |
Organism-specific databases
HPAi | ENSG00000143751, Low tissue specificity |
Interactioni
Binary interactionsi
Q6IQ49
With | #Exp. | IntAct |
---|---|---|
HSPBAP1 [Q96EW2] | 2 | EBI-2362709,EBI-720457 |
Protein-protein interaction databases
BioGRIDi | 127881, 23 interactors |
IntActi | Q6IQ49, 10 interactors |
MINTi | Q6IQ49 |
STRINGi | 9606.ENSP00000272091 |
Miscellaneous databases
RNActi | Q6IQ49, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 396 – 430 | SAP1 PublicationAdd BLAST | 35 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 182 – 208 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 222 – 362 | DisorderedSequence analysisAdd BLAST | 141 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 109 – 149 | Sequence analysisAdd BLAST | 41 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 39 – 52 | PIP-box1 PublicationAdd BLAST | 14 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 267 – 285 | Polar residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 293 – 362 | Basic and acidic residuesSequence analysisAdd BLAST | 70 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG2827, Eukaryota |
GeneTreei | ENSGT00530000063402 |
HOGENOMi | CLU_042333_0_0_1 |
InParanoidi | Q6IQ49 |
OMAi | FTNPDYQ |
OrthoDBi | 1446978at2759 |
PhylomeDBi | Q6IQ49 |
TreeFami | TF314323 |
Family and domain databases
InterProi | View protein in InterPro IPR024974, Sde2_N |
Pfami | View protein in Pfam PF13019, Sde2_N_Ubi, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAEAAALVWI RGPGFGCKAV RCASGRCTVR DFIHRHCQDQ NVPVENFFVK
60 70 80 90 100
CNGALINTSD TVQHGAVYSL EPRLCGGKGG FGSMLRALGA QIEKTTNREA
110 120 130 140 150
CRDLSGRRLR DVNHEKAMAE WVKQQAEREA EKEQKRLERL QRKLVEPKHC
160 170 180 190 200
FTSPDYQQQC HEMAERLEDS VLKGMQAASS KMVSAEISEN RKRQWPTKSQ
210 220 230 240 250
TDRGASAGKR RCFWLGMEGL ETAEGSNSES SDDDSEEAPS TSGMGFHAPK
260 270 280 290 300
IGSNGVEMAA KFPSGSQRAR VVNTDHGSPE QLQIPVTDSG RHILEDSCAE
310 320 330 340 350
LGESKEHMES RMVTETEETQ EKKAESKEPI EEEPTGAGLN KDKETEERTD
360 370 380 390 400
GERVAEVAPE ERENVAVAKL QESQPGNAVI DKETIDLLAF TSVAELELLG
410 420 430 440 450
LEKLKCELMA LGLKCGGTLQ ERAARLFSVR GLAKEQIDPA LFAKPLKGKK
K
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 162 | E → G in BAC87130 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 229 | E → D in BAC03952 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_032068 | 312 | M → I. Corresponds to variant dbSNP:rs34348128Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_024981 | 1 – 142 | MAEAA…ERLQR → MEHSLTPVTQCSMELFIVWN PDFAVEKEVLDLCSEHLVLR LRRQPIE in isoform 3. 1 PublicationAdd BLAST | 142 | |
Alternative sequenceiVSP_024982 | 53 – 64 | Missing in isoform 2. 1 PublicationAdd BLAST | 12 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK092701 mRNA Translation: BAC03952.1 AK127780 mRNA Translation: BAC87130.1 AK291008 mRNA Translation: BAF83697.1 AL133288 Genomic DNA No translation available. CH471098 Genomic DNA Translation: EAW69769.1 BC071563 mRNA Translation: AAH71563.1 |
CCDSi | CCDS41473.1 [Q6IQ49-1] |
RefSeqi | NP_689821.3, NM_152608.3 [Q6IQ49-1] |
Genome annotation databases
Ensembli | ENST00000272091; ENSP00000272091; ENSG00000143751 |
GeneIDi | 163859 |
KEGGi | hsa:163859 |
MANE-Selecti | ENST00000272091.8; ENSP00000272091.7; NM_152608.4; NP_689821.3 |
UCSCi | uc001hpu.5, human [Q6IQ49-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK092701 mRNA Translation: BAC03952.1 AK127780 mRNA Translation: BAC87130.1 AK291008 mRNA Translation: BAF83697.1 AL133288 Genomic DNA No translation available. CH471098 Genomic DNA Translation: EAW69769.1 BC071563 mRNA Translation: AAH71563.1 |
CCDSi | CCDS41473.1 [Q6IQ49-1] |
RefSeqi | NP_689821.3, NM_152608.3 [Q6IQ49-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6QDV | electron microscopy | 3.30 | z | 93-126 | [»] | |
SMRi | Q6IQ49 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 127881, 23 interactors |
IntActi | Q6IQ49, 10 interactors |
MINTi | Q6IQ49 |
STRINGi | 9606.ENSP00000272091 |
PTM databases
GlyGeni | Q6IQ49, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q6IQ49 |
MetOSitei | Q6IQ49 |
PhosphoSitePlusi | Q6IQ49 |
SwissPalmi | Q6IQ49 |
Genetic variation databases
BioMutai | SDE2 |
DMDMi | 74748828 |
Proteomic databases
EPDi | Q6IQ49 |
jPOSTi | Q6IQ49 |
MassIVEi | Q6IQ49 |
MaxQBi | Q6IQ49 |
PaxDbi | Q6IQ49 |
PeptideAtlasi | Q6IQ49 |
PRIDEi | Q6IQ49 |
ProteomicsDBi | 66484 [Q6IQ49-1] 66485 [Q6IQ49-2] 66486 [Q6IQ49-3] |
Protocols and materials databases
Antibodypediai | 34648, 98 antibodies from 17 providers |
DNASUi | 163859 |
Genome annotation databases
Ensembli | ENST00000272091; ENSP00000272091; ENSG00000143751 |
GeneIDi | 163859 |
KEGGi | hsa:163859 |
MANE-Selecti | ENST00000272091.8; ENSP00000272091.7; NM_152608.4; NP_689821.3 |
UCSCi | uc001hpu.5, human [Q6IQ49-1] |
Organism-specific databases
CTDi | 163859 |
GeneCardsi | SDE2 |
HGNCi | HGNC:26643, SDE2 |
HPAi | ENSG00000143751, Low tissue specificity |
neXtProti | NX_Q6IQ49 |
OpenTargetsi | ENSG00000143751 |
PharmGKBi | PA142672506 |
VEuPathDBi | HostDB:ENSG00000143751 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2827, Eukaryota |
GeneTreei | ENSGT00530000063402 |
HOGENOMi | CLU_042333_0_0_1 |
InParanoidi | Q6IQ49 |
OMAi | FTNPDYQ |
OrthoDBi | 1446978at2759 |
PhylomeDBi | Q6IQ49 |
TreeFami | TF314323 |
Enzyme and pathway databases
PathwayCommonsi | Q6IQ49 |
SignaLinki | Q6IQ49 |
Miscellaneous databases
BioGRID-ORCSi | 163859, 764 hits in 1047 CRISPR screens |
ChiTaRSi | SDE2, human |
GenomeRNAii | 163859 |
Pharosi | Q6IQ49, Tbio |
PROi | PR:Q6IQ49 |
RNActi | Q6IQ49, protein |
Gene expression databases
Bgeei | ENSG00000143751, Expressed in secondary oocyte and 221 other tissues |
Genevisiblei | Q6IQ49, HS |
Family and domain databases
InterProi | View protein in InterPro IPR024974, Sde2_N |
Pfami | View protein in Pfam PF13019, Sde2_N_Ubi, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SDE2_HUMAN | |
Accessioni | Q6IQ49Primary (citable) accession number: Q6IQ49 Secondary accession number(s): A8K4P3 Q8NAG7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | July 5, 2004 | |
Last modified: | February 23, 2022 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families