UniProtKB - Q6IMZ0 (NFIL3_RAT)
Nuclear factor interleukin-3-regulated protein
Nfil3
Functioni
Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B-site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death (By similarity).
Represses the transcription of CYP2A5 (By similarity).
Positively regulates the expression and activity of CES2 by antagonizing the repressive action of NR1D1 on CES2 (By similarity).
Required for the development of natural killer cell precursors (By similarity).
By similarityGO - Molecular functioni
- DNA-binding transcription factor activity Source: RGD
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: RGD
- identical protein binding Source: RGD
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: RGD
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: RGD
GO - Biological processi
- cellular response to interleukin-4 Source: RGD
- circadian rhythm Source: GO_Central
- immune response Source: InterPro
- natural killer cell differentiation Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription, DNA-templated Source: GO_Central
- transcription by RNA polymerase II Source: InterPro
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Biological rhythms, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear factor interleukin-3-regulated proteinAlternative name(s): E4 promoter-binding protein 4 |
Gene namesi | Name:Nfil3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620972, Nfil3 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Nucleus
- nucleus Source: GO_Central
- RNA polymerase II transcription regulator complex Source: RGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000292669 | 1 – 462 | Nuclear factor interleukin-3-regulated proteinAdd BLAST | 462 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 24 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 214 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 219 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 219 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 301 | PhosphoserineBy similarity | 1 | |
Cross-linki | 314 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 326 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 332 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 337 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 350 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 353 | PhosphoserineBy similarity | 1 | |
Cross-linki | 360 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 401 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 406 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 412 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 419 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 424 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 434 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 448 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | Q6IMZ0 |
PTM databases
iPTMneti | Q6IMZ0 |
PhosphoSitePlusi | Q6IMZ0 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSRNOG00000011668, Expressed in liver and 20 other tissues |
Genevisiblei | Q6IMZ0, RN |
Interactioni
Subunit structurei
Homodimer (By similarity). Binds DNA as a dimer (By similarity).
Interacts with CRY2, DR1 and PER2 (By similarity).
Interacts with NR0B2 (By similarity).
Interacts with MYSM1 (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000015525 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 73 – 136 | bZIPPROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 22 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 79 – 95 | Basic motifPROSITE-ProRule annotationAdd BLAST | 17 | |
Regioni | 99 – 106 | Leucine-zipperPROSITE-ProRule annotation | 8 | |
Regioni | 188 – 214 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 259 – 298 | DisorderedSequence analysisAdd BLAST | 40 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 188 – 207 | Polar residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 259 – 276 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 280 – 294 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3119, Eukaryota |
GeneTreei | ENSGT00940000160540 |
HOGENOMi | CLU_052045_0_0_1 |
InParanoidi | Q6IMZ0 |
OMAi | KNVHATV |
OrthoDBi | 1450431at2759 |
PhylomeDBi | Q6IMZ0 |
TreeFami | TF328374 |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR046347, bZIP_sf IPR016743, NFIL3/E4BP4 IPR010533, Vert_IL3-reg_TF |
PANTHERi | PTHR15284:SF1, PTHR15284:SF1, 1 hit |
Pfami | View protein in Pfam PF07716, bZIP_2, 1 hit PF06529, Vert_IL3-reg_TF, 1 hit |
PIRSFi | PIRSF019029, bZIP_E4BP4, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
SUPFAMi | SSF57959, SSF57959, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit PS00036, BZIP_BASIC, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MQLRKMQAIK KEPASLDPTG SSDKMLLLNS ALAEVAEDLA SGEDLLLNEG
60 70 80 90 100
SMGKNKSSAC RRKREFIPDE KKDAMYWEKR RKNNEAAKRS REKRRLNDLV
110 120 130 140 150
LENKLIALGE ENATLKAELL SLKLKFGLIS STVYAQEIQK LSNSTAVYFQ
160 170 180 190 200
DYQTSKAAVS SYVDEHEPAM VAGSCISVIK HSPQSSLSDV SEVSSVEHTQ
210 220 230 240 250
ESPAQGGCRS PENKFPVIKQ EPVELESFAR ESREERGAYS ASIYQSYMGS
260 270 280 290 300
SFSTYSHSPP LLQVHGSTSN SPRTSEADEG VVGKSSDGED EQQVPKGPIH
310 320 330 340 350
SPVELQRVHA TVVKVPEVNP SALPHKLRIK AKAMQVKVEA LDSEFEGMQK
360 370 380 390 400
LSSPADALAK RHFDLEKHGA SGTAHSSLPP FSVQVTNIQD WSLRSEHWHH
410 420 430 440 450
KELGGKTQGT FKTGVVEVKD SGYKVSEAEN LYLKQGVANL SAEVVSLKRF
460
IATQPISASD SR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 45 | L → P in AAF86615 (PubMed:11262393).Curated | 1 | |
Sequence conflicti | 144 | S → P in AAF86615 (PubMed:11262393).Curated | 1 | |
Sequence conflicti | 270 | N → S in AAF86615 (PubMed:11262393).Curated | 1 | |
Sequence conflicti | 355 | A → V in AAF86615 (PubMed:11262393).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY004663 mRNA Translation: AAF86615.1 BC072527 mRNA Translation: AAH72527.1 |
RefSeqi | NP_446179.2, NM_053727.2 XP_006253729.1, XM_006253667.3 XP_006253731.1, XM_006253669.2 XP_006253732.1, XM_006253670.3 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY004663 mRNA Translation: AAF86615.1 BC072527 mRNA Translation: AAH72527.1 |
RefSeqi | NP_446179.2, NM_053727.2 XP_006253729.1, XM_006253667.3 XP_006253731.1, XM_006253669.2 XP_006253732.1, XM_006253670.3 |
3D structure databases
AlphaFoldDBi | Q6IMZ0 |
SMRi | Q6IMZ0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000015525 |
PTM databases
iPTMneti | Q6IMZ0 |
PhosphoSitePlusi | Q6IMZ0 |
Proteomic databases
PaxDbi | Q6IMZ0 |
Genome annotation databases
Organism-specific databases
CTDi | 4783 |
RGDi | 620972, Nfil3 |
Phylogenomic databases
eggNOGi | KOG3119, Eukaryota |
GeneTreei | ENSGT00940000160540 |
HOGENOMi | CLU_052045_0_0_1 |
InParanoidi | Q6IMZ0 |
OMAi | KNVHATV |
OrthoDBi | 1450431at2759 |
PhylomeDBi | Q6IMZ0 |
TreeFami | TF328374 |
Miscellaneous databases
PROi | PR:Q6IMZ0 |
Gene expression databases
Bgeei | ENSRNOG00000011668, Expressed in liver and 20 other tissues |
Genevisiblei | Q6IMZ0, RN |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR046347, bZIP_sf IPR016743, NFIL3/E4BP4 IPR010533, Vert_IL3-reg_TF |
PANTHERi | PTHR15284:SF1, PTHR15284:SF1, 1 hit |
Pfami | View protein in Pfam PF07716, bZIP_2, 1 hit PF06529, Vert_IL3-reg_TF, 1 hit |
PIRSFi | PIRSF019029, bZIP_E4BP4, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
SUPFAMi | SSF57959, SSF57959, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit PS00036, BZIP_BASIC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NFIL3_RAT | |
Accessioni | Q6IMZ0Primary (citable) accession number: Q6IMZ0 Secondary accession number(s): Q923M2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 26, 2007 |
Last sequence update: | July 5, 2004 | |
Last modified: | May 25, 2022 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families