Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nuclear factor interleukin-3-regulated protein

Gene

Nfil3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B-site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processBiological rhythms, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor interleukin-3-regulated protein
Alternative name(s):
E4 promoter-binding protein 4
Gene namesi
Name:Nfil3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi620972 Nfil3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002926691 – 462Nuclear factor interleukin-3-regulated proteinAdd BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki24Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki214Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki219Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki219Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei301PhosphoserineBy similarity1
Cross-linki314Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki326Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki332Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki337Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki350Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei353PhosphoserineBy similarity1
Cross-linki360Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki401Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki406Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki412Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki419Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki424Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki434Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki448Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6IMZ0
PRIDEiQ6IMZ0

PTM databases

iPTMnetiQ6IMZ0
PhosphoSitePlusiQ6IMZ0

Expressioni

Inductioni

Up-regulated by dexamethasone and thapsigargin.1 Publication

Gene expression databases

BgeeiENSRNOG00000011668 Expressed in 10 organ(s), highest expression level in skeletal muscle tissue
GenevisibleiQ6IMZ0 RN

Interactioni

Subunit structurei

Homodimer. Binds DNA as a dimer. Interacts with CRY2, DR1 and PER2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015525

Structurei

3D structure databases

ProteinModelPortaliQ6IMZ0
SMRiQ6IMZ0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 136bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 95Basic motifPROSITE-ProRule annotationAdd BLAST17
Regioni99 – 106Leucine-zipperPROSITE-ProRule annotation8

Sequence similaritiesi

Belongs to the bZIP family. NFIL3 subfamily.Curated

Phylogenomic databases

eggNOGiENOG410ISXX Eukaryota
ENOG410Z2VS LUCA
GeneTreeiENSGT00530000064087
HOGENOMiHOG000059596
HOVERGENiHBG105717
InParanoidiQ6IMZ0
KOiK09059
OMAiKVEAFDH
OrthoDBiEOG091G06SA
PhylomeDBiQ6IMZ0
TreeFamiTF328374

Family and domain databases

InterProiView protein in InterPro
IPR004827 bZIP
IPR016743 NFIL3/E4BP4
IPR010533 Vert_IL3-reg_TF
PANTHERiPTHR15284:SF1 PTHR15284:SF1, 1 hit
PfamiView protein in Pfam
PF07716 bZIP_2, 1 hit
PF06529 Vert_IL3-reg_TF, 1 hit
PIRSFiPIRSF019029 bZIP_E4BP4, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
PS00036 BZIP_BASIC, 1 hit

Sequencei

Sequence statusi: Complete.

Q6IMZ0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQLRKMQAIK KEPASLDPTG SSDKMLLLNS ALAEVAEDLA SGEDLLLNEG
60 70 80 90 100
SMGKNKSSAC RRKREFIPDE KKDAMYWEKR RKNNEAAKRS REKRRLNDLV
110 120 130 140 150
LENKLIALGE ENATLKAELL SLKLKFGLIS STVYAQEIQK LSNSTAVYFQ
160 170 180 190 200
DYQTSKAAVS SYVDEHEPAM VAGSCISVIK HSPQSSLSDV SEVSSVEHTQ
210 220 230 240 250
ESPAQGGCRS PENKFPVIKQ EPVELESFAR ESREERGAYS ASIYQSYMGS
260 270 280 290 300
SFSTYSHSPP LLQVHGSTSN SPRTSEADEG VVGKSSDGED EQQVPKGPIH
310 320 330 340 350
SPVELQRVHA TVVKVPEVNP SALPHKLRIK AKAMQVKVEA LDSEFEGMQK
360 370 380 390 400
LSSPADALAK RHFDLEKHGA SGTAHSSLPP FSVQVTNIQD WSLRSEHWHH
410 420 430 440 450
KELGGKTQGT FKTGVVEVKD SGYKVSEAEN LYLKQGVANL SAEVVSLKRF
460
IATQPISASD SR
Length:462
Mass (Da):50,781
Last modified:July 5, 2004 - v1
Checksum:i1D7365C2F4B017EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45L → P in AAF86615 (PubMed:11262393).Curated1
Sequence conflicti144S → P in AAF86615 (PubMed:11262393).Curated1
Sequence conflicti270N → S in AAF86615 (PubMed:11262393).Curated1
Sequence conflicti355A → V in AAF86615 (PubMed:11262393).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY004663 mRNA Translation: AAF86615.1
BC072527 mRNA Translation: AAH72527.1
RefSeqiNP_446179.2, NM_053727.2
XP_006253729.1, XM_006253667.3
XP_006253731.1, XM_006253669.2
XP_006253732.1, XM_006253670.3
UniGeneiRn.54147

Genome annotation databases

EnsembliENSRNOT00000015525; ENSRNOP00000015525; ENSRNOG00000011668
GeneIDi114519
KEGGirno:114519

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY004663 mRNA Translation: AAF86615.1
BC072527 mRNA Translation: AAH72527.1
RefSeqiNP_446179.2, NM_053727.2
XP_006253729.1, XM_006253667.3
XP_006253731.1, XM_006253669.2
XP_006253732.1, XM_006253670.3
UniGeneiRn.54147

3D structure databases

ProteinModelPortaliQ6IMZ0
SMRiQ6IMZ0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015525

PTM databases

iPTMnetiQ6IMZ0
PhosphoSitePlusiQ6IMZ0

Proteomic databases

PaxDbiQ6IMZ0
PRIDEiQ6IMZ0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015525; ENSRNOP00000015525; ENSRNOG00000011668
GeneIDi114519
KEGGirno:114519

Organism-specific databases

CTDi4783
RGDi620972 Nfil3

Phylogenomic databases

eggNOGiENOG410ISXX Eukaryota
ENOG410Z2VS LUCA
GeneTreeiENSGT00530000064087
HOGENOMiHOG000059596
HOVERGENiHBG105717
InParanoidiQ6IMZ0
KOiK09059
OMAiKVEAFDH
OrthoDBiEOG091G06SA
PhylomeDBiQ6IMZ0
TreeFamiTF328374

Miscellaneous databases

PROiPR:Q6IMZ0

Gene expression databases

BgeeiENSRNOG00000011668 Expressed in 10 organ(s), highest expression level in skeletal muscle tissue
GenevisibleiQ6IMZ0 RN

Family and domain databases

InterProiView protein in InterPro
IPR004827 bZIP
IPR016743 NFIL3/E4BP4
IPR010533 Vert_IL3-reg_TF
PANTHERiPTHR15284:SF1 PTHR15284:SF1, 1 hit
PfamiView protein in Pfam
PF07716 bZIP_2, 1 hit
PF06529 Vert_IL3-reg_TF, 1 hit
PIRSFiPIRSF019029 bZIP_E4BP4, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
PS00036 BZIP_BASIC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNFIL3_RAT
AccessioniPrimary (citable) accession number: Q6IMZ0
Secondary accession number(s): Q923M2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again