Nfil3

Functionⁱ

Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B-site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death (By similarity).By similarity

GO - Molecular functionⁱ

DNA binding transcription factor activity
Source: RGD
Inferred from direct assayⁱ
- 11262393
identical protein binding Source: Ensembl
RNA polymerase II proximal promoter sequence-specific DNA binding Source: Ensembl
transcriptional repressor activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: GO_Central

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular response to interleukin-4 Source: Ensembl
circadian rhythm Source: GO_Central
immune response Source: InterPro
positive regulation of gene expression Source: Ensembl
transcription by RNA polymerase II Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding, Repressor
Biological process	Biological rhythms, Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Nuclear factor interleukin-3-regulated protein Alternative name(s): E4 promoter-binding protein 4
Gene namesⁱ	Name:Nfil3
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Chromosome 17

Organism-specific databases

Rat genome database More...RGDⁱ	620972 Nfil3

RGDⁱ

620972 Nfil3

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000292669	1 – 462	Nuclear factor interleukin-3-regulated proteinAdd BLAST		462

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Cross-linkⁱ	24	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	214	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	219	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linkⁱ	219	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residueⁱ	301	PhosphoserineBy similarity	1
Cross-linkⁱ	314	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	326	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	332	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	337	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	350	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	353	PhosphoserineBy similarity	1
Cross-linkⁱ	360	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	401	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	406	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	412	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	419	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	424	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	434	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	448	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbⁱ	Q6IMZ0
PRIDEⁱ	Q6IMZ0

PTM databases

iPTMnetⁱ	Q6IMZ0
PhosphoSitePlusⁱ	Q6IMZ0

Expressionⁱ

Inductionⁱ

Up-regulated by dexamethasone and thapsigargin.1 Publication

Gene expression databases

Bgeeⁱ	ENSRNOG00000011668
Genevisibleⁱ	Q6IMZ0 RN

Interactionⁱ

Subunit structureⁱ

Homodimer. Binds DNA as a dimer. Interacts with CRY2, DR1 and PER2 (By similarity).By similarity

GO - Molecular functionⁱ

identical protein binding Source: Ensembl

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	10116.ENSRNOP00000015525

STRINGⁱ

10116.ENSRNOP00000015525

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q6IMZ0
SMRⁱ	Q6IMZ0
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	73 – 136	bZIPPROSITE-ProRule annotationAdd BLAST		64

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	79 – 95	Basic motifPROSITE-ProRule annotationAdd BLAST		17
Regionⁱ	99 – 106	Leucine-zipperPROSITE-ProRule annotation		8

Sequence similaritiesⁱ

Belongs to the bZIP family. NFIL3 subfamily.Curated

Phylogenomic databases

eggNOGⁱ	ENOG410ISXX Eukaryota ENOG410Z2VS LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000064087
HOGENOMⁱ	HOG000059596
HOVERGENⁱ	HBG105717
InParanoidⁱ	Q6IMZ0
KEGG Orthology (KO) More...KOⁱ	K09059
OMAⁱ	AREPRDD
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G06SA
PhylomeDBⁱ	Q6IMZ0
TreeFamⁱ	TF328374

Family and domain databases

InterProⁱ	View protein in InterPro IPR004827 bZIP IPR016743 NFIL3/E4BP4 IPR010533 Vert_IL3-reg_TF
PANTHERⁱ	PTHR15284:SF1 PTHR15284:SF1, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07716 bZIP_2, 1 hit PF06529 Vert_IL3-reg_TF, 1 hit
PIRSFⁱ	PIRSF019029 bZIP_E4BP4, 1 hit
SMARTⁱ	View protein in SMART SM00338 BRLZ, 1 hit
PROSITEⁱ	View protein in PROSITE PS50217 BZIP, 1 hit PS00036 BZIP_BASIC, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q6IMZ0-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MQLRKMQAIK KEPASLDPTG SSDKMLLLNS ALAEVAEDLA SGEDLLLNEG 
        60         70         80         90        100
SMGKNKSSAC RRKREFIPDE KKDAMYWEKR RKNNEAAKRS REKRRLNDLV 
       110        120        130        140        150
LENKLIALGE ENATLKAELL SLKLKFGLIS STVYAQEIQK LSNSTAVYFQ 
       160        170        180        190        200
DYQTSKAAVS SYVDEHEPAM VAGSCISVIK HSPQSSLSDV SEVSSVEHTQ 
       210        220        230        240        250
ESPAQGGCRS PENKFPVIKQ EPVELESFAR ESREERGAYS ASIYQSYMGS 
       260        270        280        290        300
SFSTYSHSPP LLQVHGSTSN SPRTSEADEG VVGKSSDGED EQQVPKGPIH 
       310        320        330        340        350
SPVELQRVHA TVVKVPEVNP SALPHKLRIK AKAMQVKVEA LDSEFEGMQK 
       360        370        380        390        400
LSSPADALAK RHFDLEKHGA SGTAHSSLPP FSVQVTNIQD WSLRSEHWHH 
       410        420        430        440        450
KELGGKTQGT FKTGVVEVKD SGYKVSEAEN LYLKQGVANL SAEVVSLKRF 
       460 
IATQPISASD SR

Length:462

Mass (Da):50,781

Last modified:July 5, 2004 - v1

Checksum:ⁱ1D7365C2F4B017EF

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	45	L → P in AAF86615 (PubMed:11262393).Curated	1
Sequence conflictⁱ	144	S → P in AAF86615 (PubMed:11262393).Curated	1
Sequence conflictⁱ	270	N → S in AAF86615 (PubMed:11262393).Curated	1
Sequence conflictⁱ	355	A → V in AAF86615 (PubMed:11262393).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY004663 mRNA Translation: AAF86615.1 BC072527 mRNA Translation: AAH72527.1
NCBI Reference Sequences More...RefSeqⁱ	NP_446179.2, NM_053727.2 XP_006253729.1, XM_006253667.3 XP_006253731.1, XM_006253669.2 XP_006253732.1, XM_006253670.3
UniGeneⁱ	Rn.54147

Genome annotation databases

Ensemblⁱ	ENSRNOT00000015525; ENSRNOP00000015525; ENSRNOG00000011668
GeneIDⁱ	114519
KEGGⁱ	rno:114519

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
Q6IMZ0	Nuclear factor interleukin-3-regulated protein		MOUSE		462
Nuclear factor interleukin-3-regulated protein		MESAU		462
UPI00042AF3B2		PERMB		462
UPI0004ED59E7		NANGA		462
UPI000A30BB3B		MUSPA		462
+5

Protein	Similar proteins		Species	Score	Length
Q6IMZ0	Nuclear factor interleukin-3-regulated protein		MOUSE		462
UPI0004ED59E7		NANGA		462
UPI000A30BB3B		MUSPA		462
UPI00042AF3B2		PERMB		462
Nuclear factor interleukin-3-regulated protein		MESAU		462
+104

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY004663 mRNA Translation: AAF86615.1 BC072527 mRNA Translation: AAH72527.1
NCBI Reference Sequences More...RefSeqⁱ	NP_446179.2, NM_053727.2 XP_006253729.1, XM_006253667.3 XP_006253731.1, XM_006253669.2 XP_006253732.1, XM_006253670.3
UniGeneⁱ	Rn.54147

3D structure databases

ProteinModelPortalⁱ	Q6IMZ0
SMRⁱ	Q6IMZ0
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10116.ENSRNOP00000015525

STRINGⁱ

10116.ENSRNOP00000015525

PTM databases

iPTMnetⁱ	Q6IMZ0
PhosphoSitePlusⁱ	Q6IMZ0

Proteomic databases

PaxDbⁱ	Q6IMZ0
PRIDEⁱ	Q6IMZ0

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSRNOT00000015525; ENSRNOP00000015525; ENSRNOG00000011668
GeneIDⁱ	114519
KEGGⁱ	rno:114519

Organism-specific databases

CTDⁱ	4783
Rat genome database More...RGDⁱ	620972 Nfil3

Phylogenomic databases

eggNOGⁱ	ENOG410ISXX Eukaryota ENOG410Z2VS LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000064087
HOGENOMⁱ	HOG000059596
HOVERGENⁱ	HBG105717
InParanoidⁱ	Q6IMZ0
KEGG Orthology (KO) More...KOⁱ	K09059
OMAⁱ	AREPRDD
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G06SA
PhylomeDBⁱ	Q6IMZ0
TreeFamⁱ	TF328374

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q6IMZ0

PROⁱ

PR:Q6IMZ0

Gene expression databases

Bgeeⁱ	ENSRNOG00000011668
Genevisibleⁱ	Q6IMZ0 RN

Family and domain databases

InterProⁱ	View protein in InterPro IPR004827 bZIP IPR016743 NFIL3/E4BP4 IPR010533 Vert_IL3-reg_TF
PANTHERⁱ	PTHR15284:SF1 PTHR15284:SF1, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07716 bZIP_2, 1 hit PF06529 Vert_IL3-reg_TF, 1 hit
PIRSFⁱ	PIRSF019029 bZIP_E4BP4, 1 hit
SMARTⁱ	View protein in SMART SM00338 BRLZ, 1 hit
PROSITEⁱ	View protein in PROSITE PS50217 BZIP, 1 hit PS00036 BZIP_BASIC, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NFIL3_RAT
Accessionⁱ	Q6IMZ0Primary (citable) accession number: Q6IMZ0 Secondary accession number(s): Q923M2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
	Last sequence update:	July 5, 2004
	Last modified:	July 18, 2018
	This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

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UniProtKB - Q6IMZ0 (NFIL3_RAT)

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Nuclear factor interleukin-3-regulated protein

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

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Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ